1.1.99.22 expression in Escherichia coli BL21 as His-tag fusion protein 1.1.99.22 expression in Escherichia coli BL21(DE3)pLysS as His-tag fusion protein 1.1.99.22 gene sldBA, DNA and amino acid sequence determination and analysis 1.1.99.22 genes sldB and sldA encoding the small and the large subunit of the enzyme