1.1.3.41 DNA and amino acid sequence determination and analysis, sequence comparison, expression of His6-tagged enzyme in Escherichia coli strain MC1061 1.1.3.41 expressed in Escherichia coli C41 (DE3) cells 1.1.3.41 expressed in Escherichia coli Top10 cells 1.1.3.41 expression in Escherichia coli 1.1.3.41 functional expression of recombinant oxidase-peroxidase fusion mutant enzyme in Escherichia coli strain BL21(DE3) periplasm 1.1.3.41 gene aldO, DNA and amino acid sequence determination and analysis, sequence comparison, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) 1.1.3.41 recombinant enzyme expression in the periplasm or on the cell surface of Escherichia coli cells of different strains. The enzyme is differently tagged, i.e. expressed as Tat-AldO or INP-AldO, and exported to the periplasm or to the cell surface of the transformed cells, overview. AldO is successfully displayed at the surface of Escherichia coli using a truncated INP variant, it contains covalently bound FAD, thus has attained a correctly folded and active conformation