1.1.1.202 a synthetic pathway 1,3-propanediol production pathway is introduced in recombinant Escherichia coli consisting of glycerol dehydratase complex (dhaB123) and glycerol dehydratase reactivation factors (gdrAB) from Klebsiella pneumoniae and 1,3-propanediol oxidoreductase isoenzyme (yqhD) from Escherichia coli 747304 1.1.1.202 all genes of the dha regulon, including the gene for 1,3-propanediol oxidoreductase of Klebsiella pneumoniae mobilized by the plasmid RP4:mini Mu and transferred to Escherichia coli 389493 1.1.1.202 expressed in Escherichia coli BL21 cells 760717 1.1.1.202 expression in Clostridium acetobutylicum mutant strain DG1 from plasmid pSPD5, subcloning in Escherichia coli 667270 1.1.1.202 expression in Escherichia coli 389495, 710747, 721395, 725951 1.1.1.202 gene dhaT, cloning from genomic DNA, DNA sequence determination, inducible high-level expression of the mostly soluble enzyme in Escherichia coli strain BL21(DE3) 670216 1.1.1.202 gene dhaT, DNA and amino acid sequence determination and analysis, sequence comparisons 688171 1.1.1.202 gene dhaT, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain JM109 739886 1.1.1.202 gene dhaT, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in YqhD-deficient Escherichia coli strain BL21(DE3) 740216 1.1.1.202 gene dhaT, expression of the N-terminally His-tagged in Escherichia coli strain BL21(DE3) 684171 1.1.1.202 gene dhaT, high level co-expression of the 1,3-PD oxidoreductase with glycerol dehydratase, encoded by gene dhaB, and glycerol dehydratase reactivating factor, encoded by gene gdrAB, in Escherichia coli strain BL21 (DE3) using two incompatible plasmids, fed-batch fermentation of recombinant bacteria. The NADPH-linked alcohol dehydrogenase, which is encoded by yqhD, a gene from Escherichia coli, can non-specifically catalyze 3-hydroxypropionaldehyde converting to 1,3-propanediol with sufficient activity, overview 675901 1.1.1.202 gene dhaT, part of the dha regulon, genetic structure, overview. Coexpressions of the PDOR and GDHt from gene dhaB in Klebsiella pneumoniae result in an increase of molar yield from 50.6-64.0% of 1,3-propanediol 740214 1.1.1.202 gene dhaT, part of the dha regulon, genetic structure, recombinant expression in Escherichia coli 740214 1.1.1.202 gene dhaT, part of the dha regulon, recombinant expression in Escherichia coli 740214 1.1.1.202 gene dhaT, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) 740927 1.1.1.202 gene TM0920, expression of soluble N-terminally MGSDKIHHHHHH-tagged selenomethionine-labeled enzyme in Escherichia coli methionine auxotrophic strain DL41 657320 1.1.1.202 genes for the production of propane-1,3-diol in Clostridium butyricum, dhaB1 and dhaB2, which encode the vitamin B12-independent glycerol dehydratase DhaB1 and its activating factor, DhaB2, respectively, tandemly arrayed with the Escherichia coli yqhD gene, which encodes the 1,3-propanediol oxidoreductase isoenzyme YqhD. Heterologous expression of the propane-1,3-diol operon under the control of the temperature-sensitive lambda phage PLPR promoter regulated by the cIts857 repressor, from plasmid pDY220, in Escherichia coli K-12 ER2925 695764 1.1.1.202 overexpression in Escherichia coli 389494 1.1.1.202 overexpression of gene dhaT encoding PDOR (from vector pETPkan-dhaT) and transformed into Klebsiella pneumoniae 695716 1.1.1.202 plasmid including Escherichia coli yqhD introduced into Klebsiella pneumoniae AK mutant strain defective in in 1,3-PD oxidoreductase activity (DhaT) 695844 1.1.1.202 plasmid pUC18K-dhaT transformed into Klebsiella pneumoniae KG1 699149 1.1.1.202 plasmids pUC18 K-dhaT and pUC18 K-gdh, carrying the genes dhaT encoding PDOR and gdh encoding glycerol dehydrogenase, respectively, transformed into a propane-1,3-diol native producer Klebsiella pneumoniae KG1 696785 1.1.1.202 recombinant plasmids containing dhaT expressed in Klebsiella pneumoniae Cu, and the mutant strains AK and AR 695842 1.1.1.202 the dhaD gene encoding glycerol dehydrogenase (GDH) and dhaT gene encoding 1,3-propanediol oxidoreductase (PDOR) inserted into pTD plasmid and overexpressed in Klebsiella pneumoniae ACCC 10082 697666 1.1.1.202 the dhaT gene coding for 1,3-PD dehydrogenase inserted into vector pET-YSBLIC and transformed into Escherichia coli BL21(DE3) 698602