2.6.1.76 cloned into the expression vector pQE30 with a 6His-tag and expressed in Escherichia coli JM109 M15 cells 2.6.1.76 Escherichia coli clone carrying the dat homolog of H. influenzae 2.6.1.76 gene ectB, genetic structure, recombinant overexpression of codon-optimized version of (Pl)ectB as N-terminally Strep II-tagged protein from modified plasmid pLC52 in Escherichia coli strain BL21 and TOP 10 cells 2.6.1.76 homologs of dat detected by PCR amplification 2.6.1.76 identification and analysis of the dat gene encoding L-2,4-diaminobutyrate:2-ketoglutarate 4-aminotransferase with Escherichia coli transformants