4.2.1.34 ? enzyme consists of 2 components, neither component alone possesses hydrolase activity, MW of component I: 46000 Da, gel filtration, MW of component I: 310000 Da, gel filtration, 8 * 37000, SDS-PAGE 5668 4.2.1.34 ? the enzyme consists of two components: component I has MW 45000 and component II has MW 310000, determined by gel filtration. A combination of both components gives the active enzyme. At pH 7.5 or below component II partially dissociates into species with molecular weights of about 165000, 82000, and 37000. At pH 9.0 these species reassociate to form active component II of about the original MW. The observed species may represent a monomer, dimer, tetramer and octamer of a single subunit 5666