1.14.99.39 ? the membrane-bound, active-site-containing 27000 Da polypeptide of ammonia monooxygenase undergoes an aggregation reaction when cells or membranes are heated in the presence of SDS-PAGE. The aggregated protein can be returned to the monomeric state by incubation at high pH in the presence of SDS. Strongly hydrophobic amino acid sequences present in ammonia monooxygenase are responsible for the aggregation phenomenon 697600 1.14.99.39 ? x * 28000, subunit AmoA, SDS-PAGE -, 744103 1.14.99.39 ? x * 38000 + x * 46000, SDS-PAGE 697922 1.14.99.39 monomer 1 * 31000, SDS-PAGE -, 727350, 744760 1.14.99.39 additional information different from the beta-subunit of membrane-bound ammonia monooxygenase, the beta-subunit of soluble ammonia monooxygenase possesses an N-terminal signal sequence 696547 1.14.99.39 nonamer 3 * 27000 (alpha-subunit, AmoA) + 3 * 42000 (beta-subunit, AmoB) + 3 * 24000 (gamma-subunit, cytochrome c1), soluble enzyme, SDS-PAGE 696547