2.4.1.141 heterodimer 1 * 22600 + 1 * 27000, predicted molecular weights of subunits Alg13p and Alg14p, Alg13p is the soluble, cytosolic subunit that contains the catalytic domain, Alg14p is the membrane-spanning subunit that is required to recruit Alg13p to the cytosolic face of the endoplasmic reticulum 2.4.1.141 heterodimer 1 * 23000 + 1 * 30000, molecular weights of subunits Alg13p and Alg14p, gel filtration 2.4.1.141 additional information Alg 14 protein functions as a membrane anchor that recruits Alg 13 protein to the cytosolic face of the ER, where catalysis of GlcNAc2-PP-dol occurs. Alg 13 protein and Alg 14 protein physically interact and under normal conditions, are associated with the membrane of the endoplasmic reticulum 2.4.1.141 additional information Alg13p associates with Alg14p to a complex forming the active transferase catalyzing the biosynthesis of GlcNAc2-PP-Dol 2.4.1.141 additional information solution structure of Alg13