6.3.4.24	ATP + tyramine + L-glutamate	the enzyme participates in the biosynthesis of methanofuran, a coenzymes involved in the reduction of carbon dioxide to methane	Methanocaldococcus fervens	ADP + phosphate + gamma-glutamyltyramine	-	?	429199	
6.3.4.24	ATP + tyramine + L-glutamate	maximum activity with ATP (100%). Activities with UTP (<0.2%), CTP (<0.08%), and GTP (<0.04%) are much lower. Reactions using tyrosine or phenylalanine instead of tyramine show none of the expected products. Enzymatic reactions including gamma-glutamylglutamate, pyroglutamate, or N-acetyl-glutamate instead of L-glutamate show no expected products. No activity with furyltyramine as substrate. Arg251 is an important residue for both catalysis and glutamate binding	Methanocaldococcus fervens	ADP + phosphate + gamma-glutamyltyramine	-	?	429199	
6.3.4.24	ATP + tyramine + L-glutamate	the enzyme participates in the biosynthesis of methanofuran, a coenzymes involved in the reduction of carbon dioxide to methane	Methanocaldococcus fervens DSM 4213	ADP + phosphate + gamma-glutamyltyramine	-	?	429199	
6.3.4.24	ATP + tyramine + L-glutamate	maximum activity with ATP (100%). Activities with UTP (<0.2%), CTP (<0.08%), and GTP (<0.04%) are much lower. Reactions using tyrosine or phenylalanine instead of tyramine show none of the expected products. Enzymatic reactions including gamma-glutamylglutamate, pyroglutamate, or N-acetyl-glutamate instead of L-glutamate show no expected products. No activity with furyltyramine as substrate. Arg251 is an important residue for both catalysis and glutamate binding	Methanocaldococcus fervens DSM 4213	ADP + phosphate + gamma-glutamyltyramine	-	?	429199