6.2.1.45	ATP + Oregon Green-labeled ubiquitin + [ubiquitin-activating protein E1]-L-cysteine	enzyme efficiently accepts ubiquitin substrate fluorescently labeled by Oregon Green	Homo sapiens	AMP + diphosphate + [ubiquitin-activating protein E1]-S-(Oregon Green-labeled ubiquitinyl)-L-cysteine	-	?	428389	
6.2.1.45	ATP + SUMO2 + [ubiquitin-activating protein UBA5]-L-cysteine	SUMO2, small ubiquitin-like modifier2, an ubiquitin-like protein	Homo sapiens	AMP + diphosphate + [ubiquitin-activating protein UBA5]-S-SUMO2-L-cysteine	enzyme greatly activates SUMO2 in the nucleus or transfers activated SUMO2 to the nucleus after it conjugated SUMO2 in the cytoplasm	?	428399	
6.2.1.45	ATP + ubiquitin + SUMO2	UBE1DC1 greatly activates SUMO2 in the nucleus or transfers activated-SUMO2 to nucleus after conjugation of SUMO2 in the cytoplasm	Homo sapiens	?	-	?	400478	
6.2.1.45	ATP + ubiquitin + ubiquitin carrier protein E2	-	Homo sapiens	AMP + diphosphate + ubiquitin-(ubiquitin carrier protein E2)	-	?	502	
6.2.1.45	ATP + ubiquitin + ubiquitin-fold modifier 1	-	Homo sapiens	?	-	?	400498	
6.2.1.45	ATP + ubiquitin + Ufm1	-	Homo sapiens	?	-	?	400499	
6.2.1.45	ATP + ubiquitin + [6His-ubiquitin-activating enzyme E1]W-8His-Strep-HA	Strep, i.e.WSHPQFEK, HA, i.e. YPYDVPDYAS, under non-reducing conditions, the intermediate complex of the thioester formation is not observed without ATP	Homo sapiens	AMP + diphosphate + [6His-ubiquitin-activating enzyme E1]W-8His-Strep-HA-ubiquitinyl-L-cysteine	-	?	425986	
6.2.1.45	ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine	-	Drosophila melanogaster	AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine	-	?	432709	
6.2.1.45	ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine	-	Mus musculus	AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine	-	?	432709	
6.2.1.45	ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine	-	Homo sapiens	AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine	-	?	432709