4.6.1.13	1-phosphatidyl-1D-myo-inositol	-	Bacillus cereus	1D-myo-inositol 1,2-cyclic phosphate + diacylglycerol	-	?	368567	
4.6.1.13	1-phosphatidyl-1D-myo-inositol	natural substrate	Bacillus cereus	1D-myo-inositol 1,2-cyclic phosphate + diacylglycerol	-	?	368567	
4.6.1.13	1-phosphatidyl-1D-myo-inositol	a catalytic diad at the active site composed of Asp-274 and His-32 is involved in substrate-assisted catalysis, its function is to hydrogen-bond with the 2-OH of phosphatidylinositol to form a catalytic triad, catalytic mechanism	Bacillus cereus	1D-myo-inositol 1,2-cyclic phosphate + diacylglycerol	PI-PLC catalyzes in a second step the slow hydrolysis of 1D-myo-inositol 1,2-cyclic phosphate to form myo-inositol 1-phosphate	?	368567	
4.6.1.13	1-phosphatidyl-1D-myo-inositol	aggregated substrate is preferred over monomeric substrate	Bacillus thuringiensis	1D-myo-inositol 1,2-cyclic phosphate + diacylglycerol	a cyclic phosphodiesterase activity of PI-PLC converts 1D-myo-inositol 1,2-cyclic phosphate to inositol 1-phosphate	?	368567	
4.6.1.13	1-phosphatidyl-1D-myo-inositol	catalytic mechanism, role of Arg-69, the bidentate nature of Arg-69 is the origin of the large thio effects and stereoselectivity in PI-PLC, its function is to bring the phosphate group and the 2-OH group of inositol into proximity and to induce proper alignment for nucleophilic attack, and possibly to lower the pKa of the 2-OH	Bacillus sp. (in: Bacteria)	1D-myo-inositol 1,2-cyclic phosphate + diacylglycerol	-	?	368567	
4.6.1.13	1-phosphatidyl-1D-myo-inositol	catalyzes the cleavage of the phosphorus-oxygen bond in phosphatidylinositol, catalytic role of aspartate in a short strong hydrogen bond of the Asp274-His32 catalytic dyad, catalytic mechanism, active site structure	Bacillus thuringiensis	1D-myo-inositol 1,2-cyclic phosphate + diacylglycerol	-	?	368567	
4.6.1.13	1-phosphatidyl-1D-myo-inositol	cleaves phosphatidylinositol in a rapid intramolecular transphosphorylation reaction forming the products, in a second reaction the cyclic phosphorylase activity of PI-PLC catalyzes the slow hydrolysis of 1D-myo-inositol 1,2-cyclic phosphate to D-myo-inositol 1-phosphate, utilizes His-32 and His-82 in a general acid catalysis mechanism	Bacillus cereus	1D-myo-inositol 1,2-cyclic phosphate + diacylglycerol	-	?	368567	
4.6.1.13	1-phosphatidyl-1D-myo-inositol	general acid/general base mechanism, enhanced activity when phosphatidylinositol is present in an interface compared to monomeric substrate	Bacillus thuringiensis	1D-myo-inositol 1,2-cyclic phosphate + diacylglycerol	PI-PLC catalyzes the hydrolysis of myo-inositol 1,2-cyclic phosphate to myo-inositol 1-phosphate	?	368567	
4.6.1.13	1-phosphatidyl-1D-myo-inositol	PLC accepts only nonphosphorylated phosphatidylinositol substrates and produces cyclic inositol phosphate as final product, which is hydrolyzed at a 1000fold lower rate, catalytic mechanism, uses a guanidinium group of Arg-69 during catalysis	Bacillus sp. (in: Bacteria)	1D-myo-inositol 1,2-cyclic phosphate + diacylglycerol	-	?	368567	
4.6.1.13	1-phosphatidyl-1D-myo-inositol	the active site is located at the C-terminal side	Bacillus thuringiensis	1D-myo-inositol 1,2-cyclic phosphate + diacylglycerol	-	?	368567