3.1.26.8 5S precursor rRNA + H2O - Bacillus subtilis mature 5S rRNA - ? 370828 3.1.26.8 5S precursor rRNA + H2O - Geobacillus stearothermophilus mature 5S rRNA - ? 370828 3.1.26.8 5S precursor rRNA + H2O enzyme is responsible for maturation of 5S rRNA Bacillus subtilis mature 5S rRNA - ? 370828 3.1.26.8 5S precursor rRNA + H2O in contrast to the Escherichia coli enzyme, the Bacillus subtilis enzyme cleaves the 5S rRNA in a double-stranded region that extends from the L5 arm in one step Bacillus subtilis mature 5S rRNA - ? 370828 3.1.26.8 5S precursor rRNA + H2O 5S rRNA maturation Geobacillus stearothermophilus mature 5S rRNA - ? 370828 3.1.26.8 5S rRNA precursor + H2O - Bacillus sp. (in: Bacteria) mature 5S rRNA + oligonucleotides - ? 93123 3.1.26.8 5S rRNA precursor + H2O the enzyme cleaves 21 and 42 nucleotides respectively from the 5'-terminus and the 3'-terminus to yield the mature 116 nucleotide 5S rRNA Bacillus subtilis mature 5S rRNA + oligonucleotides the enzyme cleaves 21 and 42 nucleotides respectively from the 5'-terminus and the 3'-terminus to yield the mature 116 nucleotide 5S rRNA ? 93123 3.1.26.8 5S rRNA precursor + H2O symmetrically arranged sequences are not used in the RNase M5 interaction Bacillus subtilis mature 5S rRNA + oligonucleotides - ? 93123 3.1.26.8 5S rRNA precursor + H2O electrostatic interactions contribute about 65% to the overall binding energy Bacillus subtilis mature 5S rRNA + oligonucleotides - ? 93123 3.1.26.8 5S rRNA precursor + H2O recognition elements in potential RNase M5 substrates mainly reside in the mature moiety of the precursor. The enzyme recognizes the overall conformation of potential substrates Bacillus subtilis mature 5S rRNA + oligonucleotides - ? 93123