2.5.1.27 (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate + AMP HMBPP, the enzyme uses the hydroxylated side chain precursor to form trans-zeatin-5'-monophosphate in the reaction with AMP, reaction rate is 0.5% of the rate with dimethylallyl díphosphate Nostoc sp. diphosphate + trans-zeatin riboside 5'phosphate - ? 436641 2.5.1.27 (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate + AMP HMBPP, the enzyme uses the hydroxylated side chain precursor to form trans-zeatin-5'-monophosphate in the reaction with AMP, reaction rate is 0.5% of the rate with dimethylallyl díphosphate Nostoc sp. PCC 7120 diphosphate + trans-zeatin riboside 5'phosphate - ? 436641 2.5.1.27 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate + AMP - Agrobacterium tumefaciens diphosphate + N6-(4-hydroxy-3-methyl-2-(E)-butyl)adenosine 5'-phosphate - ? 374969 2.5.1.27 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate + AMP 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate is an intermediate in the methylerythritol phosphate pathway. Agrobacterium tumefaciens modifies cytokinin biosynthesis by sending a key enzyme into plastids of the host plant to promote tumorigenesis Agrobacterium tumefaciens diphosphate + N6-(4-hydroxy-3-methyl-2-(E)-butyl)adenosine 5'-phosphate - ? 374969 2.5.1.27 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ADP 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate is a very poor substrate Morus alba diphosphate + N6-(4-hydroxy-3-methyl-2-(E)-butenyl)ADP - ? 387460 2.5.1.27 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + AMP - Arabidopsis thaliana diphosphate + N6-(4-hydroxy-3-methyl-2-(E)-butyl)adenosine 5'-phosphate - ? 375883 2.5.1.27 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ATP 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate is a very poor substrate Morus alba trans-zeatin riboside 5'-triphosphate + diphosphate - ? 387461 2.5.1.27 dimethylallyl diphosphate + ADP the enzyme prefers ADP and ATP rather than AMP Arabidopsis thaliana ? - ? 376710 2.5.1.27 dimethylallyl diphosphate + ADP the enzyme prefers ADP and ATP rather than AMP Humulus lupulus ? - ? 376710 2.5.1.27 dimethylallyl diphosphate + ADP the enzyme preferrs ADP and ATP rather than AMP Arabidopsis thaliana dimethylallyl-ADP + diphosphate - ? 376711