1.3.99.30 1-hydroxylycopene + acceptor - Neurospora crassa 1-hydroxy-3',4'-didehydrolycopene + reduced acceptor - ? 413813 1.3.99.30 1-hydroxyneurosporene + acceptor - Neurospora crassa 1-hydroxylycopene + reduced acceptor - ? 413816 1.3.99.30 15-cis-phytoene + 5 acceptor - Neurospora crassa all-trans-3,4-didehydrolycopene + 5 reduced acceptor - ? 413171 1.3.99.30 15-cis-phytoene + 5 acceptor - Phaffia rhodozyma all-trans-3,4-didehydrolycopene + 5 reduced acceptor - ? 413171 1.3.99.30 15-cis-phytoene + 5 acceptor a decisive reaction for the formation of monocyclic or bicyclic products is the desaturation sequence to lycopene and further on to 3,4-didehydrolycopene. In the nontransformed strain, cyclization of lycopene, which directs the metabolic flux towards astaxanthin, is the dominating reaction. When the gene encoding phytoene desaturase is overexpressed, the five-step desaturation to 3,4-didehydrolycopene is intensified, resulting in an accumulation of torulene and 3-hydroxy-3'-4'-didehydro-beta-psi-caroten-4-one as subsequent products Phaffia rhodozyma all-trans-3,4-didehydrolycopene + 5 reduced acceptor - ? 413171 1.3.99.30 15-cis-phytoene + 5 acceptor the enzyme is involved in the carotenoid biosynthesis pathway to beta-carotene and torulene Neurospora crassa all-trans-3,4-didehydrolycopene + 5 reduced acceptor - ? 413171 1.3.99.30 15-cis-phytoene + 5 acceptor - Phaffia rhodozyma CBS 6938 all-trans-3,4-didehydrolycopene + 5 reduced acceptor - ? 413171 1.3.99.30 15-cis-phytoene + 5 acceptor a decisive reaction for the formation of monocyclic or bicyclic products is the desaturation sequence to lycopene and further on to 3,4-didehydrolycopene. In the nontransformed strain, cyclization of lycopene, which directs the metabolic flux towards astaxanthin, is the dominating reaction. When the gene encoding phytoene desaturase is overexpressed, the five-step desaturation to 3,4-didehydrolycopene is intensified, resulting in an accumulation of torulene and 3-hydroxy-3'-4'-didehydro-beta-psi-caroten-4-one as subsequent products Phaffia rhodozyma CBS 6938 all-trans-3,4-didehydrolycopene + 5 reduced acceptor - ? 413171 1.3.99.30 15-cis-phytoene + acceptor - Neurospora crassa all-trans-phytofluene + reduced acceptor - ? 413856 1.3.99.30 15-cis-phytoene + acceptor - Blakeslea trispora all-trans-phytofluene + reduced acceptor - ? 413856