1.2.99.8 acetaldehyde + 2,6-dichlorophenolindophenol + H2O - Sulfurisphaera tokodaii acetate + reduced 2,6-dichlorophenolindophenol - ? 420180 1.2.99.8 acetaldehyde + 2,6-dichlorophenolindophenol + H2O none of the tested electron acceptors (Sulfolobus ferredoxin, cytochrome c, NAD+, NADP+, benzoquinones, naphthoquinones) supports aldehyde oxidation as efficiently as 2,6-dichlorophenolindophenol. At pH 7.5, the enzyme exhibited activity preferentially towards the aliphatic aldehydes formaldehyde, acetaldehyde and propionaldehyde. At pH 6.7, supposed to be close to the intracellular pH of Sulfolobus, glyceraldehyde is the predominant substrate Sulfolobus acidocaldarius acetate + reduced 2,6-dichlorophenolindophenol - ? 420180 1.2.99.8 acetaldehyde + 2,6-dichlorophenolindophenol + H2O none of the tested electron acceptors (Sulfolobus ferredoxin, cytochrome c, NAD+, NADP+, benzoquinones, naphthoquinones) supports aldehyde oxidation as efficiently as 2,6-dichlorophenolindophenol. At pH 7.5, the enzyme exhibited activity preferentially towards the aliphatic aldehydes formaldehyde, acetaldehyde and propionaldehyde. At pH 6.7, supposed to be close to the intracellular pH of Sulfolobus, glyceraldehyde is the predominant substrate Sulfolobus acidocaldarius DSM 639 acetate + reduced 2,6-dichlorophenolindophenol - ? 420180 1.2.99.8 acetaldehyde + 2,6-dichlorophenolindophenol + H2O - Sulfurisphaera tokodaii DSM 16993 acetate + reduced 2,6-dichlorophenolindophenol - ? 420180 1.2.99.8 D-glyceraldehyde + H2O + 2,6-dichlorophenolindophenol - Sulfurisphaera tokodaii D-glycerate + reduced 2,6-dichlorophenolindophenol - ? 439429 1.2.99.8 D-glyceraldehyde + H2O + 2,6-dichlorophenolindophenol - Sulfurisphaera tokodaii DSM 16993 D-glycerate + reduced 2,6-dichlorophenolindophenol - ? 439429 1.2.99.8 D-glyceraldehyde + H2O + acceptor function as a glyceraldehyde oxidoreductase in the course of the nonphosphorylative Entner-Doudoroff pathway Sulfolobus acidocaldarius D-glycerate + reduced acceptor - ? 419404 1.2.99.8 D-glyceraldehyde + H2O + acceptor function as a glyceraldehyde oxidoreductase in the course of the nonphosphorylative Entner-Doudoroff pathway Sulfolobus acidocaldarius DSM 639 D-glycerate + reduced acceptor - ? 419404 1.2.99.8 D-glyceraldehyde + H2O + methyl viologen - Sulfurisphaera tokodaii D-glycerate + reduced methyl viologen - ? 439430 1.2.99.8 D-glyceraldehyde + H2O + methyl viologen - Sulfurisphaera tokodaii DSM 16993 D-glycerate + reduced methyl viologen - ? 439430