1.14.14.B11	(R)-nicotine + O2 + [reduced NADPH-hemoprotein reductase] + O2	isoforms CYP82E4, CYP82E5v2, and CYP82E10 demethylate (R)-nicotine 3, 10, and 10fold faster than (S)-nicotine, respectively	Nicotiana tabacum	(R)-nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O	-	?	427254	
1.14.14.B11	(S)-nicotine + [reduced NADPH-hemoprotein reductase] + O2	-	Nicotiana alata	(S)-nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O	-	?	427259	
1.14.14.B11	(S)-nicotine + [reduced NADPH-hemoprotein reductase] + O2	-	Nicotiana langsdorffii	(S)-nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O	-	?	427259	
1.14.14.B11	(S)-nicotine + [reduced NADPH-hemoprotein reductase] + O2	CYP82E4 selectively demethylates (S)-nicotine	Nicotiana tabacum	(S)-nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O	-	?	427259	
1.14.14.B11	(S)-nicotine + [reduced NADPH-hemoprotein reductase] + O2	only isoform CYP82E4 can significantly convert (S)-nicotine to (S)-nornicotine	Nicotiana tabacum	(S)-nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O	-	?	427259	
1.14.14.B11	additional information	isoforms CYP2A6 and CYP2B6 significantly contribute to the nicotine N-demethylation at low and high substrate concentrations, respectively	Homo sapiens	?	-	?	89	
1.14.14.B11	nicotine + NADPH + H+	-	Nicotiana tabacum	nornicotine + NADP+	-	?	442496	
1.14.14.B11	nicotine + NADPH + O2 + H+	-	Nicotiana tabacum	nornicotine + formaldehyde + NADP+ + H2O	-	?	402793	
1.14.14.B11	nicotine + NADPH + O2 + H+	demethylates nicotine to form nornicotine, a precursor to the nitrosamine N'-nitrosonornicotine	Nicotiana tabacum	nornicotine + formaldehyde + NADP+ + H2O	-	?	402793	
1.14.14.B11	nicotine + NADPH + O2 + H+	six putative substrate recognition sites	Nicotiana tabacum	nornicotine + formaldehyde + NADP+ + H2O	-	?	402793