5.4.99.19 16S rRNA uridine516 in vitro, the enzyme does not modify free 16S rRNA. The preferred substrate is a 5'-terminal fragment of 16S rRNA complexed with 30S ribosomal proteins, suggesting that pseudouridylation occurs at an intermediate stage of 30S assembly in vivo Escherichia coli 16S rRNA pseudouridine516 - ? 406993 5.4.99.19 16S rRNA uridine516 the enzyme is specific for uridine516 in 16S rRNA. In vitro, the enzyme does not modify free 16S rRNA. The preferred substrate is a 5'-terminal fragment of 16S rRNA complexed with 30S ribosomal proteins Escherichia coli 16S rRNA pseudouridine516 - ? 406993