3.4.22.B75	(di-SUMO-2)-Ran GTPase-activating protein 1 conjugate + H2O	SENP7 displays isopeptidase activity against di-SUMO-2- and SUMO-2-modified Ran GTPase-activating protein 1 but has limited activity against SUMO-1-modified Ran GTPase-activating protein 1	Homo sapiens	?	-	?	415914	
3.4.22.B75	(K335, K372, K382)-triSUMOyl-cyclic GMP-AMP synthase + 3 H2O	SUMO is conjugated onto the lysine residues 335, 372 and 382. SENP7 reverses this inhibition via catalyzing the deSUMOylation	Homo sapiens	3 SUMO + cyclic GMP-AMP synthase	-	?	449115	
3.4.22.B75	(SUMO-2)-Ran GTPase-activating protein 1 conjugate + H2O	SENP7 displays isopeptidase activity against di-SUMO-2- and SUMO-2-modified Ran GTPase-activating protein 1 but has limited activity against SUMO-1-modified Ran GTPase-activating protein 1	Homo sapiens	?	-	?	415965	
3.4.22.B75	(SUMO-3)-Ran GTPase-activating protein 1 conjugate + H2O	SENP6 and SENP7 prefer SUMO2 or SUMO3 in deconjugation reactions with rates comparable with those catalyzed by SENP2. In contrast to SENP2, SENP6 and SENP7 are less able to deconjugate SUMO1-RanGAP1, and products are only detected at enzyme concentrations 100 x higher than that observed for reactions containing SENP2	Homo sapiens	SUMO-3 + Ran GTPase-activating protein 1	-	?	415971	
3.4.22.B75	acetyl-LRGG-7-amido-4-trifluoromethylcoumarin + H2O	SENP7 prefers the LRGG sequence, residues typical of Nedd8 or ubiquitin in the P3 and P4 positions	Homo sapiens	acetyl-LRGG + 7-amino-4-trifluoromethylcoumarin	-	?	416497	
3.4.22.B75	acetyl-QTGG-7-amido-4-trifluoromethylcoumarin + H2O	very low activity	Homo sapiens	acetyl-QTGG + 7-amino-4-trifluoromethylcoumarin	-	?	416498	
3.4.22.B75	axin1 + H2O	-	Homo sapiens	?	-	?	449625	
3.4.22.B75	di-SUMO-2 + H2O	-	Homo sapiens	2 SUMO-2	-	?	416897	
3.4.22.B75	di-SUMO-3 + H2O	-	Homo sapiens	2 SUMO-3	-	?	416898	
3.4.22.B75	additional information	SENP7 has a restricted substrate specificity, being unable to process SUMO precursors and displaying paralogue-specific isopeptidase activity	Homo sapiens	?	-	?	89