2.8.1.8	biotin-PEG-Glu-Ser-Val-([8,8,8-2H3]-N6-octanoyl)Lys-Ala-Ala-Ser-As + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] ferredoxin	-	Thermus thermophilus	biotin-PEG-Glu-Ser-Val-([8,8,8-2H3]-N6-lipoyl)Lys-Ala-Ala-Ser-As + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] ferredoxin	-	?	437226	
2.8.1.8	biotin-PEG-Glu-Ser-Val-([8,8,8-2H3]-N6-octanoyl)Lys-Ala-Ala-Ser-As + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] ferredoxin	-	Escherichia coli	biotin-PEG-Glu-Ser-Val-([8,8,8-2H3]-N6-lipoyl)Lys-Ala-Ala-Ser-As + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] ferredoxin	-	?	437226	
2.8.1.8	Glu-Ser-Val-(N6-octanoyl)Lys-Ala-Ala-Ser-Asp + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] dithionite	-	Thermus thermophilus	Glu-Ser-Val-(N6-lipoyl)Lys-Ala-Ala-Ser-Asp + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] dithionite	-	?	437575	
2.8.1.8	Glu-Ser-Val-(N6-octanoyl)Lys-Ala-Ala-Ser-Asp + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] dithionite	-	Escherichia coli	Glu-Ser-Val-(N6-lipoyl)Lys-Ala-Ala-Ser-Asp + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] dithionite	-	?	437575	
2.8.1.8	H protein N6-(octanoyl)lysine + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [2Fe-2S] dithionite	-	Mycobacterium tuberculosis	H protein N6-(lipoyl)lysine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [2Fe-2S] dithionite	-	?	437598	
2.8.1.8	H protein N6-(octanoyl)lysine + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [2Fe-2S] flavodoxin	-	Mycobacterium tuberculosis	H protein N6-(lipoyl)lysine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [2Fe-2S] flavodoxin	-	?	437599	
2.8.1.8	additional information	final step in de novo biosynthesis of lipoyl cofactor. The lipoyl cofactor is essential for the function of pyruvate dehydrogenase (E2 domain)	Escherichia coli	?	-	?	89	
2.8.1.8	additional information	final step in de novo biosynthesis of lipoyl cofactor. The lipoyl cofactor is essential for the function of pyruvate dehydrogenase (E2 domain)	Neurospora crassa	?	-	?	89	
2.8.1.8	additional information	final step in de novo biosynthesis of lipoyl cofactor. The lipoyl cofactor is essential for the function of pyruvate dehydrogenase (E2 domain)	Pisum sativum	?	-	?	89	
2.8.1.8	additional information	insertion of sulfur into octanoyl groups is first at C6 to form an enzyme bound intermediate, and in a subsequent step a second sulfur is inserted at C8	Saccharolobus solfataricus	?	-	?	89