2.7.7.B5 ATP + TPHE39A truncated tRNA consisting of 39 nucleotides derived from tRNAPhe, minimal RNA substrate for modification by ThiI. The crystal structure of TPHE39A shows that base pairs in the T-stem are almost completely disrupted, although those in the acceptor-stem are preserved. ThiI can efficiently bind with not only tRNAPhe but also TPHE39A Escherichia coli adenylated TPHE39A + diphosphate - ? 411694 2.7.7.B5 additional information ThiI is involved in the modification of uridine to 4-thiouridine Escherichia coli ? - ? 89 2.7.7.B5 additional information ThiI plays a role in the transfer of sulfur from cysteine to both thiamine and 4-thiouridine Escherichia coli ? - ? 89 2.7.7.B5 tRNA-uridine + ATP - Escherichia coli adenylated-trNA-uridine + diphosphate - ? 402056 2.7.7.B5 tRNA-uridine + ATP based upon sequence alignments, ThiI shares a unique P-loop motif with the PPi synthetase family. ThiI very likely catalyzes the adenylation of a substrate, probably a molecule bearing a carbonyl oxygen Escherichia coli adenylated-trNA-uridine + diphosphate - ? 402056 2.7.7.B5 tRNA-uridine + GTP kcat/Km for GTP is about 6fold lower than the value for ATP Escherichia coli adenylated-trNA-uridine + diphosphate - ? 402057