2.4.2.29 2-amino-5-(fluoromethyl)pyrrolo[2,3-d]pyrimidin-4(3H)-one + tRNAguanine 2-amino-5-(fluoromethyl)pyrrolo[2,3-d]pyrimidin-4(3H)-one appears to partition between: 1. normal turnover, 2. inactivation, 3. an alternative processing to an unidentrified fluoride-released product Escherichia coli guanine + tRNA2-amino-5-(fluoromethyl)pyrrolo[2,3-d]pyrimidin-4(3H)-one - ? 359614 2.4.2.29 7-(cyano)-7-deazaguanine + tRNAguanine - Escherichia coli guanine + tRNA(7-(cyano)-7-deazaguanine) inserted 7-(cyano)-7-deazaguanine is located in the first position of the anticodon ? 359606 2.4.2.29 7-aminomethyl-7-carbaguanine + [RNAECYMA]-2'-deoxyguanine 34 TGT-RNA covalent intermediate Escherichia coli guanine + [RNAECYMA]-2'-deoxy-pre Q1 - ? 408227 2.4.2.29 7-aminomethyl-7-carbaguanine + [RNAECYMA]-guanine hairpin RNA corresponding to the tRNAtyr anticodon stem-loop motif, TGT-RNA covalent intermediate Escherichia coli guanine + [RNAECYMA]-pre Q1 - ? 408228 2.4.2.29 7-aminomethyl-7-carbaguanine + [tRNA]-guanine the TGT-RNA covalent complex is kinetically capable of occurring on the TGT reaction pathway. Dissociation of product RNA from the enzyme is overall rate-limiting in the steady state. Role for the 2'-hydroxyl group of the ribose in the TGT reaction Escherichia coli guanine + [tRNA]-pre Q1 - ? 408229 2.4.2.29 7-aminomethyl-7-carbaguanine + [tRNA]-guanine Tyr106 in the active site is hydrogen bonded in the ligand-free structure Zymomonas mobilis guanine + [tRNA]-pre Q1 - ? 408229 2.4.2.29 guanine + tRNAguanine - Escherichia coli tRNAguanine + guanine - r 359610 2.4.2.29 guanine34 in tRNA + 7-aminomethyl-7-carbaguanine - Escherichia coli 7-aminomethyl-7-carbaguanine34 in tRNA + guanine - ? 359605 2.4.2.29 guanine34 in tRNA + 7-aminomethyl-7-carbaguanine - Plasmodium falciparum 7-aminomethyl-7-carbaguanine34 in tRNA + guanine - ? 359605 2.4.2.29 guanine34 in tRNA + 7-aminomethyl-7-carbaguanine - Zymomonas mobilis 7-aminomethyl-7-carbaguanine34 in tRNA + guanine - ? 359605