2.4.1.288	dTDP-alpha-D-galactofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl	-	Mycobacterium tuberculosis	UDP + ?	-	?	420639	
2.4.1.288	GDP-alpha-D-glucopyranose + methyl alpha-D-mannopyrannoside	-	Leishmania major	UDP + ?	-	?	457983	
2.4.1.288	GDP-alpha-D-mannopyranose + methyl alpha-D-mannopyrannoside	-	Leishmania major	UDP + ?	-	?	457984	
2.4.1.288	additional information	GlfT2 has one active site pocket capable of catalyzing both beta-(1->5) and beta-(1->6) galactofuranosyl transfer reactions	Mycobacterium tuberculosis	?	-	?	89	
2.4.1.288	additional information	GlfT2 mediates synthesis of the galactan polymer, which contains between 20 and 40 D-galactofuranose residues connected by alternating beta-D-galactofuranosyl-(1->5)-D-galactofuranose and beta-D-galactofuranosyl-(1->6)-D-galactofuranose linkages. Elongation occurs by addition of D-galactofuranose residues from UDP-alpha-D-galactofuranose to the nonreducing end of a lipid-linked acceptor	Mycobacterium tuberculosis	?	-	?	89	
2.4.1.288	additional information	no activity with 4-phenoxy-but-2-enyl beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranoside	Mycobacterium tuberculosis	?	-	?	89	
2.4.1.288	additional information	octyl alpha-L-rhamnopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-glucopyranoside and octyl alpha-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-glucopyranoside are no effective substrates	Mycolicibacterium smegmatis	?	-	?	89	
2.4.1.288	additional information	GlfT2 is promiscuous in its substrate preferences. It preferentially acts on substrates in which the lipid-bearing Gal residue is connected to the sequence by a (1->)-beta-glycosidic linkage. The preferential formation of alternating (1->6)-beta-Galf and (1->5)-beta-Galf residues arises not from substrate binding but during catalytic processive polymerization	Mycobacterium tuberculosis	?	-	-	89	
2.4.1.288	additional information	among hexoses, monosaccharides, 4-nitrophenyl-furanoses, 4-nitorphenyl-hexoses and disaccharides tested, only methyl alpha-D-mannopyrannoside efficiently reacts as an acceptor. No donor: UDP-alpha-D-glucuronic acid	Leishmania major	?	-	-	89	
2.4.1.288	additional information	among hexoses, monosaccharides, 4-nitrophenyl-furanoses, 4-nitorphenyl-hexoses and disaccharides tested, only methyl alpha-D-mannopyrannoside efficiently reacts as an acceptor. No donors: UDP-alpha-D-glucopyranose, UDP-alpha-D-glucuronic acid, GDP-alpha-D-glucopyranose, GDP-alpha-D-mannopyranose	Leishmania major	?	-	-	89