2.1.1.270	additional information	in vivo reaction is 4'-O-methylation of isoflavones, in vitro reaction is 7-O-methylation	Medicago sativa	?	-	?	89	
2.1.1.270	additional information	in Pisum sativum, there are two highly similar hydroxymaackiain 3-O-methyltransferases HMM, with HMM1 prefering 2,7,4'-trihydroxyisoflavanone as a substrate and HMM2 being the specific O-methyltransferase that methylates (+)-6a-hydroxymaackiain	Pisum sativum	?	-	?	89	
2.1.1.270	S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain	-	Medicago truncatula	S-adenosyl-L-homocysteine + (+)-pisatin	-	?	289639	
2.1.1.270	S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain	-	Pisum sativum	S-adenosyl-L-homocysteine + (+)-pisatin	-	?	289639	
2.1.1.270	S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain	at 49% of the activity with daidzein, the enzyme also catalyzes the reaction of EC 2.1.1.150	Medicago sativa	S-adenosyl-L-homocysteine + (+)-pisatin	-	?	289639	
2.1.1.270	S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain	isoform HMM1 shows efficiencies for the methylation of 2,7,4'-trihydroxyisoflavanone 4times higher than for the methylation of (+)-6a-hydroxymaackiain, substrate of 6a-hydroxymaackiain-3-O-methyltransferase. Isoform HMM2 has a higher Vmax and lower Km on (+)-6a-hydroxymaackiain, and has a 67fold higher efficiency for the methylation of (+)-6a-hydroxymaackiain than that for 2,7,4'-trihydroxyisoflavanone	Pisum sativum	S-adenosyl-L-homocysteine + (+)-pisatin	-	?	289639	
2.1.1.270	S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain	isoform HMM2, 6a-hydroxymaackiain is the preferred substrate	Pisum sativum	S-adenosyl-L-homocysteine + (+)-pisatin	-	?	289639	
2.1.1.270	S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain	HMM2 is involved primarily in the biosynthesis of (+)-pisatin	Pisum sativum	S-adenosyl-L-homocysteine + (+)-pisatin	-	?	289639	
2.1.1.270	S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain	HMM2 shows efficiencies (relative Vmax/Km) for the 3-O-methylation of (+)-6a-hydroxymaackiain 67 times higher than for the 4'-O-methylation of 2,7,4'-trihydroxyisoflavanone	Pisum sativum	S-adenosyl-L-homocysteine + (+)-pisatin	-	?	289639	
2.1.1.270	S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain	the bifunctional enzyme also catalyzes the 4'-O-methylation of 2,7,4'-trihydroxyisoflavanonewith slightly higher catalytic efficiency	Medicago truncatula	S-adenosyl-L-homocysteine + (+)-pisatin	-	?	289639