2.1.1.173	additional information	the bifunctional methyltransferase YcbY, i.e. RlmKL, adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA, recognition of dual rRNA targets by YcbY	Escherichia coli	?	-	?	89	
2.1.1.173	additional information	helix 80 and the 12 nt ss region are critical sites necessary for m2G2445 and m7G2069 formation. Transcript 7, which lacks helix 80 and the 12 nt single-strand region, is not methylated at either position	Escherichia coli	?	-	?	89	
2.1.1.173	S-adenosyl-L-methionine + guanine2069 in 23S rRNA	-	Escherichia coli	S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA	-	?	419577	
2.1.1.173	S-adenosyl-L-methionine + guanine2069 in 23S rRNA	m7G2069 modification by YcbY is stoichiometric	Escherichia coli	S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA	-	?	419577	
2.1.1.173	S-adenosyl-L-methionine + guanine2445 in 23S rRNA	-	Escherichia coli	S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA	-	?	407348	
2.1.1.173	S-adenosyl-L-methionine + guanine2445 in 23S rRNA	N2-methylguanosine2445 of the 23S rRNA is located in a cluster of modified nucleotides concentrated at the peptidyl transferase center of the ribosome. It is likely that the G2445 modification is necessary for prevention of nonfunctional secondary or tertiary structure formation at the peptidyl transferase center	Escherichia coli	S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA	-	?	407348	
2.1.1.173	S-adenosyl-L-methionine + guanine2445 in 23S rRNA	recombinant YcbY protein is able to methylate 23S rRNA purified from the ycbY knock-out strain in vitro, assembled 50S subunits are not a substrate for the methylase	Escherichia coli	S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA	-	?	407348	
2.1.1.173	S-adenosyl-L-methionine + guanine2445 in 23S rRNA	RlmL possessing an additional RNA-binding domain acts on naked ribosomal RNA or early assembly intermediates in the cell	Escherichia coli	S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA	-	?	407348	
2.1.1.173	S-adenosyl-L-methionine + guanine2445 in 23S rRNA	duplex formation of H74 is not required for the m2G2445 formation. The 29-mer single-stranded transcript 6, which consists of residues C2422 to A2450, can form m2G2445 efficiently	Escherichia coli	S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA	-	?	407348	
2.1.1.173	S-adenosyl-L-methionine + guanine2445 in 23S rRNA	m2G2445 modification by YcbY is stoichiometric	Escherichia coli	S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA	-	?	407348