1.14.13.83 additional information biosynthesis of vitamin B12 Pseudomonas denitrificans (nom. rej.) ? - ? 89 1.14.13.83 additional information the enzyme is involved in the mechanism of the ring contraction process during vitamin B12 biosynthesis, comparison to the ring contraction process reaction pathway under anaerobic conditions in Propionibacterium freudenreichii ssp. shermanii, overview Pseudomonas denitrificans (nom. rej.) ? - ? 89 1.14.13.83 precorrin-3A + NADH + H+ + O2 - Pseudomonas denitrificans (nom. rej.) precorrin-3B + NAD+ + H2O - ? 363718 1.14.13.83 precorrin-3A + NADH + H+ + O2 first step in the aerobic pathway of viamin B12 biosynthesis Pseudomonas denitrificans (nom. rej.) precorrin-3B + NAD+ + H2O - ? 363718 1.14.13.83 precorrin-3A + NADH + H+ + O2 the Brucella melitensis enzyme is fully active in vitro (20 mM Tris-HCl buffer, pH 8.0, with 100 mM NaCl, S-adenosyl-L-methionine (SAM), 5-aminolevulinic acid (ALA) and NADPH, aerobic, dark, 4°C) and the mononuclear non-heme iron is reducible by dithionite and then is able to react with NO as oxygen analogue in the presence of the substrate Brucella melitensis precorrin-3B + NAD+ + H2O - ? 363718 1.14.13.83 precorrin-3A + NADH + H+ + O2 the Pseudomonas denitrificans enzyme is active in vivo but inactive in vitro (20 mM Tris-HCl buffer, pH 8.0, with 100 mM NaCl, S-adenosyl-L-methionine (SAM), 5-aminolevulinic acid (ALA) and NADPH, aerobic, dark, 4°C), the mononuclear non-heme iron is not reducible and probably rapidly inactivated outside the bacterium Pseudomonas denitrificans (nom. rej.) precorrin-3B + NAD+ + H2O - ? 363718 1.14.13.83 precorrin-3A + NADH + H+ + O2 the Brucella melitensis enzyme is fully active in vitro (20 mM Tris-HCl buffer, pH 8.0, with 100 mM NaCl, S-adenosyl-L-methionine (SAM), 5-aminolevulinic acid (ALA) and NADPH, aerobic, dark, 4°C) and the mononuclear non-heme iron is reducible by dithionite and then is able to react with NO as oxygen analogue in the presence of the substrate Brucella melitensis 16M precorrin-3B + NAD+ + H2O - ? 363718 1.14.13.83 precorrin-3A + NADH + O2 - Pseudomonas denitrificans (nom. rej.) precorrin-3B + NAD+ + H2O - ? 359083 1.14.13.83 precorrin-3A + NADH + O2 the enzyme is involved in ring contraction, whereby an integral carbon atom of the tetrapyrrole-derived macrocycle is removed, and cobalt chelation during aerobic synthesis of vitamin B12, the catalytic cycle of CobZ, overview Rhodobacter capsulatus precorrin-3B + NAD+ + H2O - ? 359083 1.14.13.83 precorrin-3A + NADH + O2 the enzyme generates a hydroxy lactone intermediate Rhodobacter capsulatus precorrin-3B + NAD+ + H2O - ? 359083