6.5.1.2 NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m - Staphylococcus aureus (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide - ? 444375 6.5.1.2 NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m - Haemophilus influenzae (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide - ? 444375 6.5.1.2 NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m - Escherichia coli (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide - ? 444375 6.5.1.2 NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m - Haloferax volcanii (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide - ? 444375 6.5.1.2 NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m - Streptococcus pneumoniae (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide - ? 444375 6.5.1.2 NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m - Mycobacterium tuberculosis (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide - ? 444375 6.5.1.2 NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m - Gluconobacter oxydans (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide - ? 444375 6.5.1.2 NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m - Desulfovibrio vulgaris (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide - ? 444375 6.5.1.2 NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m - Thermotoga maritima (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide - ? 444375 6.5.1.2 NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m - Mycoplasma pneumoniae (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide - ? 444375 6.5.1.2 NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m - Syntrophotalea carbinolica (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide - ? 444375 6.5.1.2 NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m - Oleidesulfovibrio alaskensis (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide - ? 444375 6.5.1.2 NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m - Synechococcus elongatus (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide - ? 444375 6.5.1.2 NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m - Wolbachia sp. subsp. Brugia malayi (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide - ? 444375 6.5.1.2 NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m - Lawsonia intracellularis (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide - ? 444375 6.5.1.2 NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m - Haemophilus influenzae ARM158 (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide - ? 444375 6.5.1.2 NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m - Escherichia coli ARC524 (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide - ? 444375 6.5.1.2 NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m - Streptococcus pneumoniae ATCC 10813 (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide - ? 444375 6.5.1.2 NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m - Mycobacterium tuberculosis H37Rv (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide - ? 444375 6.5.1.2 NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m - Wolbachia sp. subsp. Brugia malayi TRS (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide - ? 444375 6.5.1.2 NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m - Streptococcus pneumoniae ARC548 (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide - ? 444375 6.5.1.2 NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m - Staphylococcus aureus ATCC 29213 (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide - ? 444375 6.5.1.2 NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m - Staphylococcus aureus ARC561 (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide - ? 444375 6.5.1.2 ATP + (dT)10 - Thermus thermophilus ? - ? 453788 6.5.1.2 ATP + 5'-phosphate terminus of deoxyribonucleotides - Salmonella enterica subsp. enterica serovar Typhimurium ? - ? 453790 6.5.1.2 ATP + 5'-phosphate terminus of deoxyribonucleotides - Bacillus subtilis ? - ? 453790 6.5.1.2 ATP + 5'-phosphate terminus of deoxyribonucleotides - Thermus thermophilus ? - ? 453790 6.5.1.2 ATP + 5'-phosphate terminus of deoxyribonucleotides - Escherichia coli ? - ? 453790 6.5.1.2 ATP + 5'-phosphoryl termini of poly(dA-dT) - Thermus thermophilus ? - ? 453789 6.5.1.2 ATP + acridinium-ester labelled DNA - Staphylococcus aureus ? - ? 453786 6.5.1.2 ATP + DNA - Melanoplus sanguinipes ? - ? 447022 6.5.1.2 ATP + nicked DNA - Escherichia coli ? - ? 453787 6.5.1.2 ATP + nicked DNA - Thermus scotoductus ? - ? 453787 6.5.1.2 ATP + nicked DNA - Deinococcus radiodurans ? - ? 453787 6.5.1.2 ATP + nicked DNA - Aquifex aeolicus ? - ? 453787 6.5.1.2 ATP + nicked DNA - Pseudoalteromonas haloplanktis ? - ? 453787 6.5.1.2 additional information NAD+/nicotinamide nucleotide exchange reaction Escherichia coli ? - ? 89 6.5.1.2 additional information no activity with NADH, NADP, NADPH, ATP and AMP Escherichia coli ? - ? 89 6.5.1.2 additional information purified DNA ligase exhibits activity on DNA fragments with cohesive termini, and no activity is detected on blunt-end DNA. DNA ligase promotes the amplification of long DNA fragments from the genomic DNA of Thermotoga maritima Thermotoga maritima ? - ? 89 6.5.1.2 additional information the enzyme is relatively tolerant of 5'-phosphate base mispairs and 5' N:abasic lesions Escherichia coli ? - ? 89 6.5.1.2 additional information purified DNA ligase exhibits activity on DNA fragments with cohesive termini, and no activity is detected on blunt-end DNA. DNA ligase promotes the amplification of long DNA fragments from the genomic DNA of Thermotoga maritima Thermotoga maritima ATCC 43589 ? - ? 89 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Salmonella enterica subsp. enterica serovar Typhimurium ? - ? 369238 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Bacillus subtilis ? - ? 369238 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA ligase plays the key role in DNA replication of joining the nascent small pieces of DNA at the replication fork. The enzyme participates in the synthesis and repair of DNA Escherichia coli ? - ? 369238 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m the enzyme is indispensable for normal cell growth and inviability of mutants seems to be primarily the result of an inability to seal Okazaki fragments Escherichia coli ? - ? 369238 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m joining of short DNA fragments formed during DNA replication and so enabling DNA synthesis to progress in an overall 3'-5' direction on the antiparallel strand of the double helix, while continual 5'-3' synthesis proceeds on the other strand. Plays a role during genetic recombination and in the repair of UV-damaged DNA Salmonella enterica subsp. enterica serovar Typhimurium ? - ? 369238 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m joining of short DNA fragments formed during DNA replication and so enabling DNA synthesis to progress in an overall 3'-5' direction on the antiparallel strand of the double helix, while continual 5'-3' synthesis proceeds on the other strand. Plays a role during genetic recombination and in the repair of UV-damaged DNA Bacillus subtilis ? - ? 369238 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m joining of short DNA fragments formed during DNA replication and so enabling DNA synthesis to progress in an overall 3'-5' direction on the antiparallel strand of the double helix, while continual 5'-3' synthesis proceeds on the other strand. Plays a role during genetic recombination and in the repair of UV-damaged DNA Thermus thermophilus ? - ? 369238 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m joining of short DNA fragments formed during DNA replication and so enabling DNA synthesis to progress in an overall 3'-5' direction on the antiparallel strand of the double helix, while continual 5'-3' synthesis proceeds on the other strand. Plays a role during genetic recombination and in the repair of UV-damaged DNA Escherichia coli ? - ? 369238 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m the enzyme plays a pivotal role in DNA replication, repair and recombination Thermus thermophilus ? - ? 369238 6.5.1.2 NAD+ + 5'-d(ACCATTCCTGATTCTAAGTG)-3' + 5'-d(CTCAGGTCGACAGTCTGCGG)-3' 100% ligation efficiency Thermus aquaticus ? - ? 412579 6.5.1.2 NAD+ + 5'-d(ACCATTCCTGATTCTAAGTG)-3' + 5'-d(CTCAGGTCGACAGTCTGCGG)-3' 100% ligation efficiency Tequatrovirus T4 ? - ? 412579 6.5.1.2 NAD+ + 5'-d(GCCATTCCTGATTCTAAGTG)-3' + 5'-d(CTCAGGTCGACAGTCTGCGG)-3' 100% ligation efficiency Thermus aquaticus ? - ? 412580 6.5.1.2 NAD+ + 5'-d(GCCATTCCTGATTCTAAGTG)-3' + 5'-d(CTCAGGTCGACAGTCTGCGG)-3' 100% ligation efficiency Tequatrovirus T4 ? - ? 412580 6.5.1.2 NADH + (deoxyribonucleotide)n + (deoxyribonucleotide)m NADH has a significantly higher Km as NAD+ Escherichia coli ? - ? 810 6.5.1.2 Thionicotinamide derivative of NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m significantly higher Km as NAD+ Escherichia coli ? - ? 811 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Thermus aquaticus AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Thermus thermophilus AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Escherichia coli AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Tequatrovirus T4 AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Brachyspira hyodysenteriae AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Amsacta moorei entomopoxvirus AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Acanthamoeba polyphaga Mimivirus AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Aquifex aeolicus VF5 AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Enterococcus faecalis AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Sorangium cellulosum AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Acidithiobacillus ferrooxidans ATCC 23270 AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Melanoplus sanguinipes entomopoxvirus AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Invertebrate iridescent virus 6 AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Aedes taeniorhynchus iridescent virus AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Ralstonia phage phiRSL1 AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Lentisphaera araneosa HTCC2155 AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Marinobacter sp. ELB17 AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Desulfatibacillum alkenivorans AK-01 AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Desulfuromonas acetoxidans DSM 684 AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Natronomonas pharaonis DSM 2160 AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Haloquadratum walsbyi DSM 16790 AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Ostreococcus lucimarinus CCE9901 AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Rickettsia felis URRWXCal2 AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Methylacidiphilum infernorum V4 AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Nitratiruptor sp. SB155-2 AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Candidatus Blochmannia floridanus AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Leptotrichia buccalis C-1013-b AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Dictyostelium discoideum AX4 AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Borrelia hermsii HS1 AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Bacillus thuringiensis Bt407 AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Clostridium carboxidivorans P7 AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Plesiocystis pacifica SIR-1 AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Staphylococcus aureus AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m - ? 410916 6.5.1.2 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m NAD+-dependent DNA ligase LigN is non-essential for cell viability. Haloferax volcanii also encodes the ATP-dependent DNA ligase LigA. As with LigN, LigA is also non-essential for cell viability. Simultaneous inactivation of both proteins is lethal, however, indicating that they share an essential function Haloferax volcanii AMP + diphosphate + (deoxyribonucleotide)n+m - ? 804 6.5.1.2 ATP + nicked DNA - Escherichia coli AMP + diphosphate + ? - ? 453785 6.5.1.2 ATP + nicked DNA - Thermus scotoductus AMP + diphosphate + ? - ? 453785 6.5.1.2 ATP + nicked DNA - Aquifex aeolicus AMP + diphosphate + ? - ? 453785 6.5.1.2 ATP + nicked DNA - Pseudoalteromonas haloplanktis AMP + diphosphate + ? - ? 453785 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Salmonella enterica subsp. enterica serovar Typhimurium AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Staphylococcus aureus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Haemophilus influenzae AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Thermus aquaticus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Bacillus subtilis AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Thermus thermophilus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Escherichia coli AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Geobacillus stearothermophilus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Streptococcus pneumoniae AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Mycobacterium tuberculosis AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Streptomyces coelicolor AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Helicobacter pylori AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Thermus scotoductus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Thermus sp. AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Mycoplasma pneumoniae AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Deinococcus radiodurans AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Thermus filiformis AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Aquifex aeolicus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Melanoplus sanguinipes AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Aquifex pyrophilus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Amsacta moorei entomopoxvirus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Enterococcus faecalis AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m - Pseudoalteromonas haloplanktis AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m joining of the 5'-phosphoryl of a DNA chain to the 3'-hydroxyl of an RNA molecule in two ways: 1. Oligo(A) is joined to the 5'-phosphoryl of oligo(dA) on a poly(dT) template strand. 2. the enzyme can circularize poly[d(A-T)]pU in which UMP occupies the 3'-hydroxyl terminal position Escherichia coli AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m (dT)8, (dT)10, (dT)12-18 Thermus thermophilus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m joining of a single-strand-scission in double-stranded DNA Bacillus amyloliquefaciens AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m blunt-end ligation, intermolecular joining to yield linear oligomers, but no circular DNA forms in presence of high concentrations of polyethylene glycols Thermus thermophilus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m blunt-end ligation in presence of macromolecular solutes, inactive in absence Escherichia coli AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m oligonucleotides as short as six or seven in length can be joined if annealed to long complementary deoxyribonucleotides Escherichia coli AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m joining of 5'-phosphoryl terminus of DNA chain to the 3'-hydroxyl terminus of RNA Escherichia coli AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA ligase requires two DNA termini, the 5'-terminus, carrying a phosphate group, and the 3'-terminus, a hydroxyl group. These termini must reside on a double-stranded molecule, DNA:DNA or DNA:RNA. Both strands of the duplex may terminate, in the form of a staggered end or a blunt end, and the ligase then requires a second similar double-stranded terminus to join the two in an intermolecular reaction. Alternatively, the two termini may be provided by a nick in just one strand of a duplex, which the enzyme will then seal Salmonella enterica subsp. enterica serovar Typhimurium AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA ligase requires two DNA termini, the 5'-terminus, carrying a phosphate group, and the 3'-terminus, a hydroxyl group. These termini must reside on a double-stranded molecule, DNA:DNA or DNA:RNA. Both strands of the duplex may terminate, in the form of a staggered end or a blunt end, and the ligase then requires a second similar double-stranded terminus to join the two in an intermolecular reaction. Alternatively, the two termini may be provided by a nick in just one strand of a duplex, which the enzyme will then seal Bacillus subtilis AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA ligase requires two DNA termini, the 5'-terminus, carrying a phosphate group, and the 3'-terminus, a hydroxyl group. These termini must reside on a double-stranded molecule, DNA:DNA or DNA:RNA. Both strands of the duplex may terminate, in the form of a staggered end or a blunt end, and the ligase then requires a second similar double-stranded terminus to join the two in an intermolecular reaction. Alternatively, the two termini may be provided by a nick in just one strand of a duplex, which the enzyme will then seal Thermus thermophilus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA ligase requires two DNA termini, the 5'-terminus, carrying a phosphate group, and the 3'-terminus, a hydroxyl group. These termini must reside on a double-stranded molecule, DNA:DNA or DNA:RNA. Both strands of the duplex may terminate, in the form of a staggered end or a blunt end, and the ligase then requires a second similar double-stranded terminus to join the two in an intermolecular reaction. Alternatively, the two termini may be provided by a nick in just one strand of a duplex, which the enzyme will then seal Escherichia coli AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m cohesive-end DNA restriction fragments Thermus thermophilus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m bacteriophage T5 DNA, which contains single strand interruptions at defined positions in one of the complementary strands Bacillus amyloliquefaciens AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m formation of linear oligomers by intermolecular cohesive-end ligation Escherichia coli AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m the self-complementary polymer, poly(dA-dT), forms a looped-back structure that DNA ligase can join to yield a circular molecule Escherichia coli AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m joins oligo(dT) that is base-paired to poly(dA) or oligo(dA) base-paired to poly(dT) Escherichia coli AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m PvuII endonuclease-digested pBR322 DNA, formation of linear oligomers ranging from dimer through pentamer Escherichia coli AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m formation of linear oligomers by intermolecular blunt-end ligation in presence of high concentrations of polymer polyethylene glycol Escherichia coli AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m nick-closing activity Thermus thermophilus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m nick-closing activity Thermus scotoductus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m nick-closing activity Rhodothermus marinus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m catalyzes the joining of polynucleotide strands provided they have juxtaposed 3'-hydroxyl and 5'-phosphoryl end groups aligned in a duplex structure: e.g. annealed ends of lamdda DNA, endogenous nicks in T5 DNA, interruptions created by the action of pancreatic DNAse, annealed fragments generated by the staggered cutting action of some restriction endonucleases Escherichia coli AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m no ligation of blunt-ended or flush-ended DNAs Escherichia coli AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m - ? 809 6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m activity depends on the base pairs on both sides of the nick, T/A