6.5.1.2	ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m	NAD+-dependent DNA ligase LigN is non-essential for cell viability. Haloferax volcanii also encodes the ATP-dependent DNA ligase LigA. As with LigN, LigA is also non-essential for cell viability. Simultaneous inactivation of both proteins is lethal, however, indicating that they share an essential function	Haloferax volcanii	AMP + diphosphate + (deoxyribonucleotide)n+m	-	?	804	
6.5.1.2	ATP + (dT)10	-	Thermus thermophilus	?	-	?	453788	
6.5.1.2	ATP + 5'-phosphate terminus of deoxyribonucleotides	-	Salmonella enterica subsp. enterica serovar Typhimurium	?	-	?	453790	
6.5.1.2	ATP + 5'-phosphate terminus of deoxyribonucleotides	-	Bacillus subtilis	?	-	?	453790	
6.5.1.2	ATP + 5'-phosphate terminus of deoxyribonucleotides	-	Thermus thermophilus	?	-	?	453790	
6.5.1.2	ATP + 5'-phosphate terminus of deoxyribonucleotides	-	Escherichia coli	?	-	?	453790	
6.5.1.2	ATP + 5'-phosphoryl termini of poly(dA-dT)	-	Thermus thermophilus	?	-	?	453789	
6.5.1.2	ATP + acridinium-ester labelled DNA	-	Staphylococcus aureus	?	-	?	453786	
6.5.1.2	ATP + DNA	-	Melanoplus sanguinipes	?	-	?	447022	
6.5.1.2	ATP + nicked DNA	-	Escherichia coli	AMP + diphosphate + ?	-	?	453785	
6.5.1.2	ATP + nicked DNA	-	Thermus scotoductus	AMP + diphosphate + ?	-	?	453785	
6.5.1.2	ATP + nicked DNA	-	Aquifex aeolicus	AMP + diphosphate + ?	-	?	453785	
6.5.1.2	ATP + nicked DNA	-	Pseudoalteromonas haloplanktis	AMP + diphosphate + ?	-	?	453785	
6.5.1.2	ATP + nicked DNA	-	Escherichia coli	?	-	?	453787	
6.5.1.2	ATP + nicked DNA	-	Thermus scotoductus	?	-	?	453787	
6.5.1.2	ATP + nicked DNA	-	Deinococcus radiodurans	?	-	?	453787	
6.5.1.2	ATP + nicked DNA	-	Aquifex aeolicus	?	-	?	453787	
6.5.1.2	ATP + nicked DNA	-	Pseudoalteromonas haloplanktis	?	-	?	453787	
6.5.1.2	additional information	NAD+/nicotinamide nucleotide exchange reaction	Escherichia coli	?	-	?	89	
6.5.1.2	additional information	no activity with NADH, NADP, NADPH, ATP and AMP	Escherichia coli	?	-	?	89	
6.5.1.2	additional information	purified DNA ligase exhibits activity on DNA fragments with cohesive termini, and no activity is detected on blunt-end DNA. DNA ligase promotes the amplification of long DNA fragments from the genomic DNA of Thermotoga maritima	Thermotoga maritima	?	-	?	89	
6.5.1.2	additional information	the enzyme is relatively tolerant of 5'-phosphate base mispairs and 5' N:abasic lesions	Escherichia coli	?	-	?	89	
6.5.1.2	additional information	purified DNA ligase exhibits activity on DNA fragments with cohesive termini, and no activity is detected on blunt-end DNA. DNA ligase promotes the amplification of long DNA fragments from the genomic DNA of Thermotoga maritima	Thermotoga maritima ATCC 43589	?	-	?	89	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Salmonella enterica subsp. enterica serovar Typhimurium	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Staphylococcus aureus	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Haemophilus influenzae	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Thermus aquaticus	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Bacillus subtilis	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Thermus thermophilus	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Escherichia coli	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Geobacillus stearothermophilus	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Streptococcus pneumoniae	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Mycobacterium tuberculosis	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Streptomyces coelicolor	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Helicobacter pylori	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Thermus scotoductus	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Thermus sp.	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Mycoplasma pneumoniae	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Deinococcus radiodurans	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Thermus filiformis	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Aquifex aeolicus	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Melanoplus sanguinipes	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Aquifex pyrophilus	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Amsacta moorei entomopoxvirus	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Enterococcus faecalis	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Pseudoalteromonas haloplanktis	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	joining of the 5'-phosphoryl of a DNA chain to the 3'-hydroxyl of an RNA molecule in two ways: 1. Oligo(A) is joined to the 5'-phosphoryl of oligo(dA) on a poly(dT) template strand. 2. the enzyme can circularize poly[d(A-T)]pU in which UMP occupies the 3'-hydroxyl terminal position	Escherichia coli	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	(dT)8, (dT)10, (dT)12-18	Thermus thermophilus	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	joining of a single-strand-scission in double-stranded DNA	Bacillus amyloliquefaciens	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	blunt-end ligation, intermolecular joining to yield linear oligomers, but no circular DNA forms in presence of high concentrations of polyethylene glycols	Thermus thermophilus	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	blunt-end ligation in presence of macromolecular solutes, inactive in absence	Escherichia coli	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	oligonucleotides as short as six or seven in length can be joined if annealed to long complementary deoxyribonucleotides	Escherichia coli	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	joining of 5'-phosphoryl terminus of DNA chain to the 3'-hydroxyl terminus of RNA	Escherichia coli	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	DNA ligase requires two DNA termini, the 5'-terminus, carrying a phosphate group, and the 3'-terminus, a hydroxyl group. These termini must reside on a double-stranded molecule, DNA:DNA or DNA:RNA. Both strands of the duplex may terminate, in the form of a staggered end or a blunt end, and the ligase then requires a second similar double-stranded terminus to join the two in an intermolecular reaction. Alternatively, the two termini may be provided by a nick in just one strand of a duplex, which the enzyme will then seal	Salmonella enterica subsp. enterica serovar Typhimurium	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	DNA ligase requires two DNA termini, the 5'-terminus, carrying a phosphate group, and the 3'-terminus, a hydroxyl group. These termini must reside on a double-stranded molecule, DNA:DNA or DNA:RNA. Both strands of the duplex may terminate, in the form of a staggered end or a blunt end, and the ligase then requires a second similar double-stranded terminus to join the two in an intermolecular reaction. Alternatively, the two termini may be provided by a nick in just one strand of a duplex, which the enzyme will then seal	Bacillus subtilis	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	DNA ligase requires two DNA termini, the 5'-terminus, carrying a phosphate group, and the 3'-terminus, a hydroxyl group. These termini must reside on a double-stranded molecule, DNA:DNA or DNA:RNA. Both strands of the duplex may terminate, in the form of a staggered end or a blunt end, and the ligase then requires a second similar double-stranded terminus to join the two in an intermolecular reaction. Alternatively, the two termini may be provided by a nick in just one strand of a duplex, which the enzyme will then seal	Thermus thermophilus	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	DNA ligase requires two DNA termini, the 5'-terminus, carrying a phosphate group, and the 3'-terminus, a hydroxyl group. These termini must reside on a double-stranded molecule, DNA:DNA or DNA:RNA. Both strands of the duplex may terminate, in the form of a staggered end or a blunt end, and the ligase then requires a second similar double-stranded terminus to join the two in an intermolecular reaction. Alternatively, the two termini may be provided by a nick in just one strand of a duplex, which the enzyme will then seal	Escherichia coli	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	cohesive-end DNA restriction fragments	Thermus thermophilus	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	bacteriophage T5 DNA, which contains single strand interruptions at defined positions in one of the complementary strands	Bacillus amyloliquefaciens	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	formation of linear oligomers by intermolecular cohesive-end ligation	Escherichia coli	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	the self-complementary polymer, poly(dA-dT), forms a looped-back structure that DNA ligase can join to yield a circular molecule	Escherichia coli	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	joins oligo(dT) that is base-paired to poly(dA) or oligo(dA) base-paired to poly(dT)	Escherichia coli	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	PvuII endonuclease-digested pBR322 DNA, formation of linear oligomers ranging from dimer through pentamer	Escherichia coli	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	formation of linear oligomers by intermolecular blunt-end ligation in presence of high concentrations of polymer polyethylene glycol	Escherichia coli	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	nick-closing activity	Thermus thermophilus	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	nick-closing activity	Thermus scotoductus	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	nick-closing activity	Rhodothermus marinus	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	catalyzes the joining of polynucleotide strands provided they have juxtaposed 3'-hydroxyl and 5'-phosphoryl end groups aligned in a duplex structure: e.g. annealed ends of lamdda DNA, endogenous nicks in T5 DNA, interruptions created by the action of pancreatic DNAse, annealed fragments generated by the staggered cutting action of some restriction endonucleases	Escherichia coli	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	no ligation of blunt-ended or flush-ended DNAs	Escherichia coli	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	activity depends on the base pairs on both sides of the nick, T/A<G/C<C/G<A/T, no ligation of gapped substrates	Aquifex aeolicus	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA	Staphylococcus aureus	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA	Thermus aquaticus	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA	Bacillus subtilis	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA	Escherichia coli	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA	Geobacillus stearothermophilus	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA	Mycobacterium tuberculosis	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA	Streptomyces coelicolor	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA	Thermus scotoductus	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA	Thermus filiformis	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA	Aquifex aeolicus	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA	Melanoplus sanguinipes	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA	Aquifex pyrophilus	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA	Amsacta moorei entomopoxvirus	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA	Pseudoalteromonas haloplanktis	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	LigA is essential for growth of Mycobacterium tuberculosis	Mycobacterium tuberculosis	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	acridinium-ester labelled DNA	Staphylococcus aureus	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	cohesive ends of restriction endonuclease digested lambda-DNA	Thermus thermophilus HB8	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	the enzyme seals sticky ends efficiently, but has little activity on 1-nucleotide gap or blunt-ended DNA substrates even in presence of polyethylene glycol. MsEPV DNA ligase readily joins DNA nicks with mismatches at either side of the nick junction, except for mismatches at the nick junction containing an A base in the template strand (A/A, G/A, and C/A). The enzyme can join DNA probes on RNA templates. MsEPV DNA ligase joins mismatches at the 3' side of the nick more frequently than at the 5' side of the nick on an RNA template. No activity with ATP	Melanoplus sanguinipes	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	although NAD+-dependent DNA ligase is essential for mycobacterial viability, only low levels of protein are required for growth	Mycolicibacterium smegmatis	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	in addition to the unique N-terminal domain Ia that stimulates ligase-AMP formation, there are three C-terminal domains that extend from the OB domain: a small zinc-binding (Zn) domain, a helix-hairpin-helix domain, and a BRCA1 C-terminal domain	Escherichia coli	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	in addition to the unique N-terminal domain Ia that stimulates ligase-AMP formation, there are three C-terminal domains that extend from the OB domain: a small zinc-binding (Zn) domain, a helix-hairpin-helix domain, and a BRCA1 C-terminal domain	Enterococcus faecalis	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	in addition to the unique N-terminal domain Ia that stimulates ligase-AMP formation, there are three C-terminal domains that extend from the OB domain: a small zinc-binding (Zn) domain, a helix-hairpin-helix domain, and a BRCA1 C-terminal domain	Thermus filiformis	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	Hind III digested plasmid	Thermotoga maritima	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Streptomyces coelicolor A3(2)	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA	Streptomyces coelicolor A3(2)	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Mycobacterium tuberculosis H37Rv	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA	Mycobacterium tuberculosis H37Rv	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Mycoplasma pneumoniae M29	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Escherichia coli MG1655	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Haemophilus influenzae KW20	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Thermus sp. TAK16D	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	Hind III digested plasmid	Thermotoga maritima ATCC 43589	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Staphylococcus aureus RN4220	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	809	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Salmonella enterica subsp. enterica serovar Typhimurium	?	-	?	369238	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Bacillus subtilis	?	-	?	369238	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	DNA ligase plays the key role in DNA replication of joining the nascent small pieces of DNA at the replication fork. The enzyme participates in the synthesis and repair of DNA	Escherichia coli	?	-	?	369238	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	the enzyme is indispensable for normal cell growth and inviability of mutants seems to be primarily the result of an inability to seal Okazaki fragments	Escherichia coli	?	-	?	369238	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	joining of short DNA fragments formed during DNA replication and so enabling DNA synthesis to progress in an overall 3'-5' direction on the antiparallel strand of the double helix, while continual 5'-3' synthesis proceeds on the other strand. Plays a role during genetic recombination and in the repair of UV-damaged DNA	Salmonella enterica subsp. enterica serovar Typhimurium	?	-	?	369238	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	joining of short DNA fragments formed during DNA replication and so enabling DNA synthesis to progress in an overall 3'-5' direction on the antiparallel strand of the double helix, while continual 5'-3' synthesis proceeds on the other strand. Plays a role during genetic recombination and in the repair of UV-damaged DNA	Bacillus subtilis	?	-	?	369238	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	joining of short DNA fragments formed during DNA replication and so enabling DNA synthesis to progress in an overall 3'-5' direction on the antiparallel strand of the double helix, while continual 5'-3' synthesis proceeds on the other strand. Plays a role during genetic recombination and in the repair of UV-damaged DNA	Thermus thermophilus	?	-	?	369238	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	joining of short DNA fragments formed during DNA replication and so enabling DNA synthesis to progress in an overall 3'-5' direction on the antiparallel strand of the double helix, while continual 5'-3' synthesis proceeds on the other strand. Plays a role during genetic recombination and in the repair of UV-damaged DNA	Escherichia coli	?	-	?	369238	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	the enzyme plays a pivotal role in DNA replication, repair and recombination	Thermus thermophilus	?	-	?	369238	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Thermus aquaticus	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Thermus thermophilus	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Escherichia coli	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Tequatrovirus T4	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Brachyspira hyodysenteriae	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Amsacta moorei entomopoxvirus	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Acanthamoeba polyphaga Mimivirus	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Aquifex aeolicus VF5	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Enterococcus faecalis	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Sorangium cellulosum	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Acidithiobacillus ferrooxidans ATCC 23270	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Melanoplus sanguinipes entomopoxvirus	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Invertebrate iridescent virus 6	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Aedes taeniorhynchus iridescent virus	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Ralstonia phage phiRSL1	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Lentisphaera araneosa HTCC2155	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Marinobacter sp. ELB17	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Desulfatibacillum alkenivorans AK-01	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Desulfuromonas acetoxidans DSM 684	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Natronomonas pharaonis DSM 2160	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Haloquadratum walsbyi DSM 16790	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Ostreococcus lucimarinus CCE9901	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Rickettsia felis URRWXCal2	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Methylacidiphilum infernorum V4	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Nitratiruptor sp. SB155-2	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Candidatus Blochmannia floridanus	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Leptotrichia buccalis C-1013-b	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Dictyostelium discoideum AX4	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Borrelia hermsii HS1	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Bacillus thuringiensis Bt407	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Clostridium carboxidivorans P7	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Plesiocystis pacifica SIR-1	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Staphylococcus aureus	AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m	-	?	410916	
6.5.1.2	NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m	-	Staphylococcus aureus	(deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide	-	?	444375	
6.5.1.2	NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m	-	Haemophilus influenzae	(deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide	-	?	444375	
6.5.1.2	NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m	-	Escherichia coli	(deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide	-	?	444375	
6.5.1.2	NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m	-	Haloferax volcanii	(deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide	-	?	444375	
6.5.1.2	NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m	-	Streptococcus pneumoniae	(deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide	-	?	444375	
6.5.1.2	NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m	-	Mycobacterium tuberculosis	(deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide	-	?	444375	
6.5.1.2	NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m	-	Gluconobacter oxydans	(deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide	-	?	444375	
6.5.1.2	NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m	-	Desulfovibrio vulgaris	(deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide	-	?	444375	
6.5.1.2	NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m	-	Thermotoga maritima	(deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide	-	?	444375	
6.5.1.2	NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m	-	Mycoplasma pneumoniae	(deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide	-	?	444375	
6.5.1.2	NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m	-	Syntrophotalea carbinolica	(deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide	-	?	444375	
6.5.1.2	NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m	-	Oleidesulfovibrio alaskensis	(deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide	-	?	444375	
6.5.1.2	NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m	-	Synechococcus elongatus	(deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide	-	?	444375	
6.5.1.2	NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m	-	Wolbachia sp. subsp. Brugia malayi	(deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide	-	?	444375	
6.5.1.2	NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m	-	Lawsonia intracellularis	(deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide	-	?	444375	
6.5.1.2	NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m	-	Haemophilus influenzae ARM158	(deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide	-	?	444375	
6.5.1.2	NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m	-	Escherichia coli ARC524	(deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide	-	?	444375	
6.5.1.2	NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m	-	Streptococcus pneumoniae ATCC 10813	(deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide	-	?	444375	
6.5.1.2	NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m	-	Mycobacterium tuberculosis H37Rv	(deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide	-	?	444375	
6.5.1.2	NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m	-	Wolbachia sp. subsp. Brugia malayi TRS	(deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide	-	?	444375	
6.5.1.2	NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m	-	Streptococcus pneumoniae ARC548	(deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide	-	?	444375	
6.5.1.2	NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m	-	Staphylococcus aureus ATCC 29213	(deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide	-	?	444375	
6.5.1.2	NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m	-	Staphylococcus aureus ARC561	(deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide	-	?	444375	
6.5.1.2	NAD+ + 5'-d(ACCATTCCTGATTCTAAGTG)-3' + 5'-d(CTCAGGTCGACAGTCTGCGG)-3'	100% ligation efficiency	Thermus aquaticus	?	-	?	412579	
6.5.1.2	NAD+ + 5'-d(ACCATTCCTGATTCTAAGTG)-3' + 5'-d(CTCAGGTCGACAGTCTGCGG)-3'	100% ligation efficiency	Tequatrovirus T4	?	-	?	412579	
6.5.1.2	NAD+ + 5'-d(GCCATTCCTGATTCTAAGTG)-3' + 5'-d(CTCAGGTCGACAGTCTGCGG)-3'	100% ligation efficiency	Thermus aquaticus	?	-	?	412580	
6.5.1.2	NAD+ + 5'-d(GCCATTCCTGATTCTAAGTG)-3' + 5'-d(CTCAGGTCGACAGTCTGCGG)-3'	100% ligation efficiency	Tequatrovirus T4	?	-	?	412580	
6.5.1.2	NAD+ + deoxyribonucleotide	-	Streptococcus pneumoniae	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	427493	
6.5.1.2	NAD+ + deoxyribonucleotide	-	Wolbachia endosymbiont of Brugia malayi	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?	427493	
6.5.1.2	NADH + (deoxyribonucleotide)n + (deoxyribonucleotide)m	NADH has a significantly higher Km as NAD+	Escherichia coli	?	-	?	810	
6.5.1.2	Thionicotinamide derivative of NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	significantly higher Km as NAD+	Escherichia coli	?	-	?	811