6.3.4.10 ATP + biotin + apo-beta-methylcrotonyl-CoA carboxylase - Homo sapiens AMP + diphosphate + holo-beta-methylcrotonyl-CoA carboxylase - ? 372442 6.3.4.10 ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - Gallus gallus AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 665 6.3.4.10 ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - eukaryota AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 665 6.3.4.10 ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - Homo sapiens AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 665 6.3.4.10 ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - Rattus norvegicus AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 665 6.3.4.10 ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - Saccharomyces cerevisiae AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 665 6.3.4.10 ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - Bos taurus AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 665 6.3.4.10 ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - Oryctolagus cuniculus AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 665 6.3.4.10 ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - Pisum sativum AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 665 6.3.4.10 ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - Arabidopsis thaliana AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 665 6.3.4.10 ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] biotinyl-AMP is an intermediate Oryctolagus cuniculus AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 665 6.3.4.10 ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] biotinylates apopropionyl-CoA carboxylase from patients with holocarboxylase synthetase deficiency Bos taurus AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 665 6.3.4.10 ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] the alpha-subunit of propionyl-CoA carboxylase and its mature counterpart are competent for biotinylation Rattus norvegicus AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 665 6.3.4.10 ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - Oryctolagus cuniculus ? - ? 369342 6.3.4.10 ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] plays an essential role in biotin utilization eukaryota ? - ? 369342 6.3.4.10 ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] its deficiency causes biotin-responsive multiple carboxylase deficiency in humans Homo sapiens ? - ? 369342 6.3.4.10 ATP + biotin + apo-[propionyl CoA carboxylase] - Homo sapiens AMP + diphosphate + propionyl CoA carboxylase - ? 407124 6.3.4.10 ATP + biotin + apo-[propionyl-CoA carboxylase] - Homo sapiens AMP + diphosphate + propionyl-CoA carboxylase - ? 407125 6.3.4.10 ATP + biotin + apo-[propionyl-CoA carboxylase] substrate is p67, the carboxyl-terminal fragment of the propionyl-CoA-carboxylase alpha subunit, the substrate is biotinylated at Lys67 Homo sapiens AMP + diphosphate + propionyl-CoA carboxylase - ? 407125 6.3.4.10 ATP + biotin + apo-[propionyl-CoA carboxylase] substrate is p67, the carboxyl-terminal fragment of the propionyl-CoA-carboxylase alpha subunit, the substrate is biotinylated at Lys67. Determination of amino acid of HCS involved in the interaction, docking of p67 near Arg508 and Ser515 within the central domain of HCS, overview Homo sapiens AMP + diphosphate + propionyl-CoA carboxylase - ? 407125 6.3.4.10 ATP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)] HCS catalyzes the incorporation of biotin into carboxylases, holo-PCC is formed from the apo-PCC Homo sapiens AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 372443 6.3.4.10 ATP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)] HCS catalyzes the incorporation of biotin into carboxylases, holo-PCC is formed from the apo-PCC from biotin-deficient rat liver or human lymphoblast in the presence of d-biotin and ATP, much higher activity with apo-PCC from rat livers than from human lymphoblasts Bos taurus AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 372443 6.3.4.10 ATP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)] - Homo sapiens AMP + diphosphate + [propionyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 427372 6.3.4.10 ATP + biotin + apoacetyl-CoA carboxylase biotinylation of cytosolic ACC Homo sapiens AMP + diphosphate + holoacetyl-CoA carboxylase - ? 372444 6.3.4.10 ATP + biotin + apocarboxyl carrier protein HCS catalyzes the incorporation of biotin into carboxylases, recombinant apo-CCP from Escherichia coli Bos taurus AMP + diphosphate + holocarboxyl carrier protein - ? 372445 6.3.4.10 ATP + biotin + apocarboxylase - Homo sapiens AMP + diphosphate + holocarboxylase - ? 407126 6.3.4.10 ATP + biotin + apopropionyl-CoA carboxylase biotinylation of mitochondrial PCC Homo sapiens AMP + diphosphate + holopropionyl-CoA carboxylase - ? 372446 6.3.4.10 ATP + biotin + apopyruvate carboxylase - Homo sapiens AMP + diphosphate + holopyruvate carboxylase - ? 372447 6.3.4.10 ATP + biotin + BCCP87 substrate is the biotinoyl domain, BCCP87, of Escherichia coli biotin carboxyl carrier protein, BCCP, binding pattern, overview Homo sapiens AMP + diphosphate + biotin-BCCP87 - ? 408473 6.3.4.10 ATP + biotin + histone H2A pattern of biotin attachment and comparison to nonenzymatic biotinylation of histone H2A, none of the lysine sites within histone H2A resembles the biotin attachment consensus sequence seen in carboxylases, mechanism, overview. H2A is a poor substrate for biotin attachment compared to the apocarboxylase biotin-attachment domain Homo sapiens AMP + diphosphate + biotin-histone H2A - ? 408474 6.3.4.10 ATP + biotin + histone H3.2 - Homo sapiens AMP + diphosphate + biotin-histone H3.2 - ? 416610 6.3.4.10 ATP + biotin + histone H3.2 - Homo sapiens AMP + diphosphate + biotin-histone H3.2 biotinylation of residues K9 and K18 in histone H3 ? 416610 6.3.4.10 ATP + biotin + histone H3.2 N-terminal amino acids 1-25 - Homo sapiens AMP + diphosphate + biotin-histone H3.2 N-terminal amino acids 1-25 biotinylation of residues K9 and K18 ? 416609 6.3.4.10 ATP + biotin + histone H4 biotinylation at Lys12 especially in telomeric repeats, overview Homo sapiens AMP + diphosphate + biotin-histone H4 - ? 407127 6.3.4.10 ATP + biotin + p67 comprises the 67 C-terminal amino acids in human propionyl-CoA carboxylase, including the biotin-binding site lysine-669 Homo sapiens AMP + diphosphate + biotinyl-p67 - ? 416611 6.3.4.10 ATP + biotin + protein Syn67 mutated Syn67, both N- and C-termini of HCS interact with Syn67, a polypeptide with a basic patch of lysines and arginines, and the Syn67 docking site is located near the active pocket of HCS. The target lysine in Syn67 is biotinylated by HCS only after arginine-to-glycine substitutions in Syn67 produce a histone-like peptide, binding structure, modelling, overview Homo sapiens AMP + diphosphate + protein Syn67-biotin - ? 408475 6.3.4.10 biotinyl-5'-AMP + apocarboxylase - Oryctolagus cuniculus AMP + holocarboxylase - ? 453766 6.3.4.10 CTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - Oryctolagus cuniculus CMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 667 6.3.4.10 CTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] as active as ATP Bos taurus CMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 667 6.3.4.10 CTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] 19% of the activity relative to ATP Oryctolagus cuniculus CMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 667 6.3.4.10 CTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] 117% of the activity relative to ATP Bos taurus CMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 667 6.3.4.10 CTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] 28% of the activity relative to ATP Gallus gallus CMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 667 6.3.4.10 CTP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)] CTP can totally replace ATP Bos taurus CMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 372780 6.3.4.10 GTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - Gallus gallus GMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 668 6.3.4.10 GTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - Oryctolagus cuniculus GMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 668 6.3.4.10 GTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] 50% of the activity relative to ATP Bos taurus GMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 668 6.3.4.10 GTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] 11% of the activity relative to ATP Oryctolagus cuniculus GMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 668 6.3.4.10 GTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] 46% of the activity relative to ATP Bos taurus GMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 668 6.3.4.10 GTP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)] GTP is approximately half as active as ATP Bos taurus GMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 373347 6.3.4.10 ITP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - Gallus gallus IMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 669 6.3.4.10 ITP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] 50% of the activity relative to ATP Bos taurus IMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 669 6.3.4.10 ITP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] 47% of the activity relative to ATP Bos taurus IMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 669 6.3.4.10 ITP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)] ITP is approximately half as active as ATP Bos taurus IMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 373506 6.3.4.10 additional information HCS catalyzes the ATP-dependent biotinylation of the four biotin-dependent carboxylases found in humans Homo sapiens ? - ? 89 6.3.4.10 additional information the enzyme is critical to utilization of exogenous or dietary biotin and responds to biotin deprivation by reduction in the steady state level of their mRNAs. The free biotin is directed to brain because the reduction in holocarboxylase synthetase, sodium-dependent multivitamin-transporter and carboxylase mRNAs in liver reduces their capacity to utilize biotin while leaving free biotin in circulation Rattus norvegicus ? - ? 89 6.3.4.10 additional information although mammals have multiple biotin-dependent enzymes there is only a single gene encoding holocarboxylase synthetase, HCS, responsible for all cellular biotinylation. The enzyme catalyzes the reactions of EC 6.3.4.10, EC 6.3.4.11, and EC 6.3.4.15, overview Homo sapiens ? - ? 89 6.3.4.10 additional information holocarboxylase synthetase catalyzes the attachment of biotin to the epsilon-amino group of a specific lysine residue in apocarboxylases, biotinylation of biotin-dependent carboxylases requires ATP and proceeds in the following two steps Homo sapiens ? - ? 89 6.3.4.10 additional information holocarboxylase synthetase, HCS, catalyzes the binding of biotin to lysines in carboxylases and histones in two steps. First, HCS catalyzes the synthesis of biotinyl-5'-AMP, and second, the biotinyl moiety is ligated to lysine residues, binding structure, modelling, overview Homo sapiens ? - ? 89 6.3.4.10 additional information the enzyme catalyzes the reactions of EC 6.3.4.10, EC 6.3.4.11, and EC 6.3.4.15, overview Homo sapiens ? - ? 89 6.3.4.10 additional information the 4'-hydroxyazobenzene-2-carboxylic acid dye assay can be used to quantify biotin Homo sapiens ? - ? 89 6.3.4.10 additional information the N-terminal domain of hHCS recognizes the charged region of biotin acceptor protein, distinctly from the recognition by the catalytic domain Homo sapiens ? - ? 89 6.3.4.10 UTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] 24% of the activity relative to ATP Oryctolagus cuniculus UMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 666 6.3.4.10 UTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] 95% of the activity relative to ATP Gallus gallus UMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] - ? 666