6.3.2.43	ATP + an [amino group carrier protein]-C-terminal-L-glutamate + L-2-aminoadipate	-	Thermococcus kodakarensis	ADP + phosphate + an [amino group carrier protein]-C-terminal-N-(1,5-dicarboxypentan-1-yl)-L-glutamine	-	?	458515	
6.3.2.43	ATP + an [amino group carrier protein]-C-terminal-L-glutamate + L-2-aminoadipate	the amino-group carrier protein is LysW. Determination of the crystal structure of the LysX family protein from Thermococcus kodakarensis, which catalyzes the conjugation of LysW with either alpha-aminoadipate (AAA) or glutamate, in a complex with LysW-gamma-AAA. Substrate binding structure, overview. Residues Thr196, Asn250, and Ala251 are involved in the recognition of the delta-carboxyl group of AAA	Thermococcus kodakarensis	ADP + phosphate + an [amino group carrier protein]-C-terminal-N-(1,5-dicarboxypentan-1-yl)-L-glutamine	-	?	458515	
6.3.2.43	ATP + [lysine-biosynthesis-protein LysW]-C-terminal-L-glutamate + L-2-aminoadipate	-	Thermococcus kodakarensis	ADP + phosphate + [lysine-biosynthesis-protein LysW]-C-terminal-gamma-(L-2-aminoadip-2-yl)-L-glutamate	-	?	443214	
6.3.2.43	ATP + [lysine-biosynthesis-protein LysW]-C-terminal-L-glutamate + L-2-aminoadipate	-	Thermococcus kodakarensis JCM 12380	ADP + phosphate + [lysine-biosynthesis-protein LysW]-C-terminal-gamma-(L-2-aminoadip-2-yl)-L-glutamate	-	?	443214	
6.3.2.43	ATP + [lysine-biosynthesis-protein LysW]-C-terminal-L-glutamate + L-2-aminoadipate	-	Thermococcus kodakarensis ATCC BAA-918	ADP + phosphate + [lysine-biosynthesis-protein LysW]-C-terminal-gamma-(L-2-aminoadip-2-yl)-L-glutamate	-	?	443214	
6.3.2.43	ATP + [lysine-biosynthesis-protein LysW]-C-terminal-L-glutamate + L-glutamate	-	Thermococcus kodakarensis	ADP + phosphate + [lysine-biosynthesis-protein LysW]-C-terminal-gamma-(L-glutam-2-yl)-L-glutamate	-	?	443215	
6.3.2.43	ATP + [LysW mutant E42R]-C-terminal-L-glutamate + L-2-aminoadipate	-	Thermococcus kodakarensis	ADP + phosphate + [LysW mutant E42R]-C-terminal-N-(1,5-dicarboxypentan-1-yl)-L-glutamine	-	?	459487	
6.3.2.43	ATP + [LysW]-C-terminal-L-glutamate + L-2-aminoadipate	lysine biosynthesis. Protection of the amino group of L-2-aminoadipate by conjugation to the carrier protein LysW	Sulfolobus acidocaldarius	ADP + phosphate + [LysW]-gamma-(L-2-aminoadip-2-yl)-L-glutamate	-	?	424678	
6.3.2.43	ATP + [LysW]-C-terminal-L-glutamate + L-2-aminoadipate	lysine biosynthesis. Protection of the amino group of L-2-aminoadipate by conjugation to the carrier protein LysW	Sulfolobus acidocaldarius DSM 639	ADP + phosphate + [LysW]-gamma-(L-2-aminoadip-2-yl)-L-glutamate	-	?	424678	
6.3.2.43	ATP + [LysW]-C-terminal-L-glutamate + L-2-aminoadipate	mutant enzyme N264G/T265T shows a substrate preference for glutamate over L-2-aminoadipate contrasting distinctly with the substrate specificity of wild-type enzyme, which shows a distinct preference for L-2-aminoadipate over glutamate	Sulfolobus acidocaldarius	ADP + phosphate + [LysW]-C-terminal-gamma-(L-2-aminoadip-2-yl)-L-glutamate	-	?	425989	
6.3.2.43	ATP + [LysW]-C-terminal-L-glutamate + L-2-aminoadipate	mutant enzyme N264G/T265T shows a substrate preference for glutamate over L-2-aminoadipate contrasting distinctly with the substrate specificity of wild-type enzyme, which shows a distinct preference for L-2-aminoadipate over glutamate	Sulfolobus acidocaldarius DSM 639	ADP + phosphate + [LysW]-C-terminal-gamma-(L-2-aminoadip-2-yl)-L-glutamate	-	?	425989	
6.3.2.43	ATP + [LysW]-C-terminal-L-glutamate + L-2-aminoadipate	-	Thermococcus kodakarensis	ADP + phosphate + [LysW]-C-terminal-N-(1,4-dicarboxybutan-1-yl)-L-glutamine	-	?	459488	
6.3.2.43	ATP + [LysW]-C-terminal-L-glutamate54 + L-2-aminoadipate	the enzyme is involved in biosynthesis of lysine. LysX activates the gamma-carboxyl group of the C-terminal Glu54 of the 2-L-aminoaadipate carrier-protein LysW by phosphorylation, with concomitant conversion of ATP to ADP. LysX recognizes not only the C-terminal glutamate residue of LysW but also other portions, possibly the globular domain of LysW specifically	Thermus thermophilus	ADP + phosphate + [LysW]-C-terminal-gamma-(L-2-aminoadip-2-yl)-L-glutamate54	-	?	424680	
6.3.2.43	ATP + [LysW]-C-terminal-L-glutamate54 + L-2-aminoadipate	LysX activates the gamma-carboxyl group of the C-terminal Glu54 of the 2-L-aminoaadipate carrier-protein LysW by phosphorylation, with concomitant conversion of ATP to ADP. LysX recognizes not only the C-terminal glutamate residue of LysW but also other portions, possibly the globular domain of LysW specifically	Thermus thermophilus	ADP + phosphate + [LysW]-C-terminal-gamma-(L-2-aminoadip-2-yl)-L-glutamate54	-	?	424680	
6.3.2.43	additional information	purified recombinant TK0278 recognizes alpha-aminoadipate (AAA) and glutamate as substrates and ligated both compounds with TK0279, an amino group carrier protein LysW in this microorganism, in an ATP-dependent manner	Thermococcus kodakarensis	?	-	-	89	
6.3.2.43	additional information	purified recombinant TK0278 recognizes alpha-aminoadipate (AAA) and glutamate as substrates and ligated both compounds with TK0279, an amino group carrier protein LysW in this microorganism, in an ATP-dependent manner	Thermococcus kodakarensis JCM 12380	?	-	-	89	
6.3.2.43	additional information	purified recombinant TK0278 recognizes alpha-aminoadipate (AAA) and glutamate as substrates and ligated both compounds with TK0279, an amino group carrier protein LysW in this microorganism, in an ATP-dependent manner	Thermococcus kodakarensis ATCC BAA-918	?	-	-	89