5.4.99.33 (3S)-2,3-epoxy-2,3-dihydrosqualene - Cucurbita pepo 444088 5.4.99.33 (3S)-2,3-epoxy-2,3-dihydrosqualene - Siraitia grosvenorii 444088 5.4.99.33 (3S)-2,3-epoxy-2,3-dihydrosqualene - Citrullus colocynthis 444088 5.4.99.33 (S)-squalene-2,3-epoxide - Cucurbita pepo 415958 5.4.99.33 additional information GC-MS metabolite identification and analysis, extracts from Saccharomyces cerevisiae transformants expressing enzyme SgCbQ produce only one new peak, that is identified as cucurbitadienol Siraitia grosvenorii 89 5.4.99.33 additional information 11-oxo-24,25-epoxy cucurbitadienol might be a derivative of 24,25-epoxy cucurbitadienol, which is assumed to be formed through cyclization of 2,3-(S)-22,23-(S)-dioxidosqualene in yeast cells by recombinant SgCbQ, and then sequentially oxidized by recombinant CYP87D18 (EC 1.14.14.71) at the C-11 position in BY4741-Z5 as the oxidation process of cucurbitadienol. Metabolite identification by TLC, metabolite structures are confirmed by liquid chromatography-solid-phase extraction-nuclear magnetic resonance-mass spectrometry coupling (LC-SPE-NMR-MS) Siraitia grosvenorii 89