5.4.99.12 Escherichia coli tRNAPhe uridine39 - Escherichia coli Escherichia coli tRNAPhe pseudouridine39 - ? 412033 5.4.99.12 human tRNALeu uridine 38 uridine39 - Mus musculus human tRNALeu pseudouridine 38 pseudouridine39 psuedouridine formation in position 39 is clearly preferred over position 38 ? 412180 5.4.99.12 human tRNALys3 - Mus musculus ? - ? 412181 5.4.99.12 human tRNASer uridine39 - Mus musculus human tRNASer pseudouridine39 - ? 412182 5.4.99.12 additional information presence of a G36 residue modulates modification at position 38. In addition to local sequence effects, steady-state kinetic analyses suggest the existence of other recognition elements distinct from the immediate vicinity of modification Escherichia coli ? - ? 89 5.4.99.12 Salmonella typhimurium HisT- tRNALeu tRNAPhe2 from mutant HisT- strain defective for tRNA pseudouridine synthase I that forms pseudouridine in the 3'-side of the anticodon region of approximately half of the cellular tRNAs Bos taurus ? - ? 412848 5.4.99.12 Salmonella typhimurium HisT- tRNAPhe2 uridine39 tRNAPhe2 from mutant HisT- strain defective for tRNA pseudouridine synthase I that forms pseudouridine in the 3’ side of the anticodon region of approximately half of the cellular tRNAs Bos taurus Salmonella typhimurium HisT- tRNAPhe2 pseudouridine39 specific modification of uridine39 ? 412849 5.4.99.12 Salmonelly typhimurium HisT- tRNATyr tRNAPhe2 from mutant HisT- strain defective for tRNA pseudouridine synthase I that forms pseudouridine in the 3'-side of the anticodon region of approximately half of the cellular tRNAs Bos taurus ? - ? 412850 5.4.99.12 tRNA uridine38 - Saccharomyces cerevisiae tRNA pseudouridine38 - ? 410406 5.4.99.12 tRNA uridine38 tRNALeu3 contains uridine at position 38. Wild-typeTruA pseudouridylates uridines at all three positions (38, 39 and 40) with efficiencies (kcat/KM) differing by less than 10fold, while R58A is inactive toward all three uridines. When flexibility of the anticodon stem loop is increased by mutating the two G:C base pairs in the stem of the anticodon stem loop of tRNALeu3 into A:U pairs, the kcat/KM increased 2fold. When flexibility is decreased by base-pairing the target U38 of tRNALeu3 with A32 instead of with U32, the kcat/KM decreases 10fold Escherichia coli tRNA pseudouridine38 - ? 410406 5.4.99.12 tRNA uridine38 the enzyme modifies the anticodon arm of transfer RNA at positions 38 and 39 by catalyzing the conversion of uridine to pseudouridine Saccharomyces cerevisiae tRNA pseudouridine38 - ? 410406 5.4.99.12 tRNA uridine38-40 - Thermus thermophilus tRNA pseudouridine38-40 - ? 407413 5.4.99.12 tRNA uridine38-40 TruA specifically modifies uridines at positions 38, 39, and/or 40 of tRNAs with highly divergent sequences and structures Escherichia coli tRNA pseudouridine38-40 - ? 407413 5.4.99.12 tRNA uridine38-40 purified tRNA pseudouridine synthase I modifies all of the hisT isoacceptors of tRNAHis, tRNATyr, and tRNALeu to products which are chromatographically indistinguishable from the respective wild-type species. These three groups of isoacceptors contain all the known topological sites for pseudouridine modification of residues 38,39, and 40 Escherichia coli tRNA pseudouridine38-40 - ? 407413 5.4.99.12 tRNA uridine38-40 TruA specifically modifies uridines at positions 38, 39, and/or 40 of tRNAs with highly divergent sequences and structures. The molecular basis for the site and substrate promiscuity is studied by determining the crystal structures of Eschrichia coli TruA in complex with two different leucyl tRNAs in conjunction with functional assays and computer simulation Escherichia coli tRNA pseudouridine38-40 - ? 407413 5.4.99.12 tRNA uridine38-40 - Thermus thermophilus HB8 / ATCC 27634 / DSM 579 tRNA pseudouridine38-40 - ? 407413 5.4.99.12 tRNA uridine39 - Saccharomyces cerevisiae tRNA pseudouridine39 - ? 410407 5.4.99.12 tRNA uridine39 modified tRNALeu3 with uridine at position 39 instead of position 38. Wild-typeTruA pseudouridylates uridines at all three positions (38, 39 and 40) with efficiencies (kcat/KM) differing by less than 10fold, while R58A is inactive toward all three uridines Escherichia coli tRNA pseudouridine39 - ? 410407 5.4.99.12 tRNA uridine39 tRNAPhe from yeast, which contains a single target for tRNA pseudouridine synthase I at U39 Escherichia coli tRNA pseudouridine39 - ? 410407 5.4.99.12 tRNA uridine39 tRNAPhe with uridine at position 39 Escherichia coli tRNA pseudouridine39 - ? 410407 5.4.99.12 tRNA uridine39 the enzyme modifies the anticodon arm of transfer RNA at positions 38 and 39 by catalyzing the conversion of uridine to pseudouridine Saccharomyces cerevisiae tRNA pseudouridine39 - ? 410407 5.4.99.12 tRNA uridine39 with tRNA substrates from both yeast and humans, uridines at position 39 are modified to pseudouridine. In a tRNA substrate with a uridine at position 38 (human tRNALeu), there is very slight formation of pseudouridine at that position after incubation with mPus3p Mus musculus tRNA pseudouridine39 - ? 410407 5.4.99.12 tRNA uridine40 modified tRNALeu3 with uridine at position 40 instead of position 38. Wild-typeTruA pseudouridylates uridines at all three positions (38, 39 and 40) with efficiencies (kcat/KM) differing by less than 10fold, while R58A is inactive toward all three uridines Escherichia coli tRNA pseudouridine40 - ? 410408 5.4.99.12 tRNAHis guanidine36 uridine38 cytidine39 - Escherichia coli RNAHis guanidine36 pseudouridine38 cytidine39 - ? 413006 5.4.99.12 tRNAHis uridine38 cytidine39 - Escherichia coli RNAHis pseudouridine38 cytidine39 - ? 413007 5.4.99.12 tRNAHis uridine38 uridine39 - Escherichia coli tRNAHis pseudouridine38 pseudouridine39 - ? 413008 5.4.99.12 tRNALeu3 - Escherichia coli ? - ? 413012 5.4.99.12 tRNALeu3 carrying uridine at position 38 - Escherichia coli tRNALeu3 carrying pseudouridine at position 38 - ? 413009 5.4.99.12 tRNALeu3 carrying uridine at position 39 - Escherichia coli tRNALeu3 carrying pseudouridine at position 39 - ? 413010 5.4.99.12 tRNALeu3 carrying uridine at position 40 - Escherichia coli tRNALeu3 carrying pseudouridine at position 40 - ? 413011 5.4.99.12 tRNAVal2a cytidine36 uridine38 poor substrate, tRNAVal2a is not a substrate Escherichia coli tRNAVal2a cytidine36 pseudouridine38 - ? 413014 5.4.99.12 yeast tRNAPhe - Mus musculus ? - ? 413080