5.3.3.2 (Z)-3-(difluoromethyl)-2-buten-1-yl diphosphate about 2% of the activity with isopenteyl diphosphate Thermus thermophilus ? - ? 399239 5.3.3.2 (Z)-3-(fluoromethyl)-2-buten-1-yl diphosphate about 2% of the activity with isopenteyl diphosphate Thermus thermophilus ? - ? 399240 5.3.3.2 3-(fluoromethyl)-3-buten-1-yl diphosphate competition between isomerization and alkylation of the flavin cofactor Thermus thermophilus ? - ? 399522 5.3.3.2 3-cyclopropyl-3-buten-1-yl diphosphate - Staphylococcus aureus (2Z)-3-cyclopropylbut-2-en-1-yl diphosphate - r 444688 5.3.3.2 3-Ethylbut-3-enyl diphosphate ir Sus scrofa trans-3-Methylpent-3-enyl diphosphate - ? 1089 5.3.3.2 3-methylene-4-penten-1-yl diphosphate competition between isomerization and alkylation of the flavin cofactor Thermus thermophilus ? - ? 399547 5.3.3.2 isopentenyl diphosphate - Gallus gallus dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Staphylococcus aureus dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Staphylococcus aureus dimethylallyl diphosphate - r 1088 5.3.3.2 isopentenyl diphosphate - Bacillus subtilis dimethylallyl diphosphate - r 1088 5.3.3.2 isopentenyl diphosphate - Thermus thermophilus dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Thermus thermophilus dimethylallyl diphosphate - r 1088 5.3.3.2 isopentenyl diphosphate - Escherichia coli dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Escherichia coli dimethylallyl diphosphate - r 1088 5.3.3.2 isopentenyl diphosphate - Homo sapiens dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Rattus norvegicus dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Sus scrofa dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Saccharomyces cerevisiae dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Bombyx mori dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Methanothermobacter thermautotrophicus dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Pinus radiata dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Streptococcus pneumoniae dimethylallyl diphosphate - r 1088 5.3.3.2 isopentenyl diphosphate - Schizosaccharomyces pombe dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Ricinus communis dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Aedes aegypti dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Solanum lycopersicum dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Solanum lycopersicum dimethylallyl diphosphate - r 1088 5.3.3.2 isopentenyl diphosphate - Capsicum annuum dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Cucurbita sp. dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Citrus sp. dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Acholeplasma laidlawii dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Haematococcus lacustris dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Claviceps purpurea dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Claviceps sp. dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Narcissus pseudonarcissus dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Salvia officinalis dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Phaffia rhodozyma dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Saccharolobus shibatae dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Methanocaldococcus jannaschii dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Synechocystis sp. dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Catharanthus roseus dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Manduca sexta dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Thermococcus kodakarensis dimethylallyl diphosphate - r 1088 5.3.3.2 isopentenyl diphosphate - Choristoneura fumiferana dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Populus trichocarpa dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Mesocricetus auratus dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Jatropha curcas dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Lycium chinense dimethylallyl diphosphate - r 1088 5.3.3.2 isopentenyl diphosphate - Salvia miltiorrhiza dimethylallyl diphosphate - r 1088 5.3.3.2 isopentenyl diphosphate r Saccharomyces cerevisiae dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate r Cinchona robusta dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate the enzyme catalyses the interconversion of isopentenyl diphosphate and dimethylallyl diphosphate with a rather low degree of stereochemical fidelity. At least three kinetically distinguishable exchange processes are detected Saccharomyces cerevisiae dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate the enzyme catalyzes a crucial activation step in the isoprenoid biosynthesis pathway Escherichia coli dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate the enzyme catalyzes the interconversion of the fundamental five carbon homoallylic and allylic diphosphate building blocks required for biosynthesis of isoprenoid compounds Methanothermobacter thermautotrophicus dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate accumulation of a reduced neutral dihydroflavin intermediate upon incubation of the reduced enzyme with isopentenyl diphosphate or dimethylallyl diphosphate. The intermediate forms and decays at kinetically competent rates in the pre-steady-state Staphylococcus aureus dimethylallyl diphosphate - r 1088 5.3.3.2 isopentenyl diphosphate a mechanism is proposed where the reduced flavin cofactor acts as a general acid/base catalyst and helps stabilize the carbocationic intermediate formed by protonation Saccharolobus shibatae dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate steady-state equilibrium ratio of isopentenyl diphosphate/dimethylallyl diphosphate of approximately 1:7 Artemisia annua dimethylallyl diphosphate - r 1088 5.3.3.2 isopentenyl diphosphate - Claviceps sp. SD58 dimethylallyl diphosphate - ? 1088 5.3.3.2 isopentenyl diphosphate - Streptococcus pneumoniae CGSP14 dimethylallyl diphosphate - r 1088 5.3.3.2 isopentenyl diphosphate - Staphylococcus aureus IDI-2 dimethylallyl diphosphate - ? 1088 5.3.3.2 Isopentenyl diphosphate - Saccharomyces cerevisiae ? - ? 369363 5.3.3.2 Isopentenyl diphosphate - Schizosaccharomyces pombe ? - ? 369363 5.3.3.2 Isopentenyl diphosphate essential activation step in isoprenoid biosynthetic pathway Homo sapiens ? - ? 369363 5.3.3.2 additional information the enzyme also has NADH dehydrogenase activity although it catalyzes the isomerase reaction without consuming any detectable amount of NADH. O2 acts as electron acceptor in the NADH dehydrogenase reaction Saccharolobus shibatae ? - ? 89 5.3.3.2 additional information zinc is located in an unusual six-coordinate pocket and may facilitate protonation of the unactivated carbon-carbon double bond in isopentenyl diphosphate. The sulfhydryl moiety C67, perhaps in the thiolate form, is in position to remove a proton from the resulting tertiary carbocation to complete the reaction Escherichia coli ? - ? 89 5.3.3.2 additional information enzyme of the isoprenoid pathway Ricinus communis ? - ? 89 5.3.3.2 additional information enzyme in the pathway of conversion of isopentenyl diphosphate to phytoene Solanum lycopersicum ? - ? 89 5.3.3.2 additional information enzyme in the pathway of conversion of isopentenyl diphosphate to phytoene Narcissus pseudonarcissus ? - ? 89 5.3.3.2 additional information induced after addition of a homogenate of the phytopathogenic fungus Phytophthora cinnamomi. Enzyme may be involved in the biosynthesis of anthraquinone phytoalexins that accumulate rapidly after elicitation of Cinchona cells Cinchona robusta ? - ? 89 5.3.3.2 additional information key enzyme in biosynthesis of isoprenoids Escherichia coli ? - ? 89 5.3.3.2 additional information substrate binds to the inactive oxidized and active reduced form of enzyme with similar affinities. Substrate-dependent accumulation of the neutral flavin semiquinone during both the flavoenzyme reduction and reoxidation processes Thermus thermophilus ? - ? 89 5.3.3.2 additional information 3-butyn-1-yl diphosphate and 2,3-butadien-1-yl diphosphate are no substrates for IDI-1 Escherichia coli ? - ? 89 5.3.3.2 additional information 3-butyn-1-yl diphosphate and 2,3-butadien-1-yl diphosphate are no substrates for IDI-2 Thermus thermophilus ? - ? 89 5.3.3.2 additional information ìsoform IDI-2 catalyzes exchange of the vinyl hydrogens in 1-OPP without concomitant isomerization Thermus thermophilus ? - ? 89 5.3.3.2 pyruvate - Bacillus subtilis lactate - ? 380235 5.3.3.2 trans-3-Methylpent-3-enyl diphosphate r Sus scrofa trans-3-Methylpent-2-enyl diphosphate - ? 1090