5.1.3.30 D-fructose - Treponema primitia D-psicose - r 428519 5.1.3.30 D-fructose - Ruminiclostridium cellulolyticum D-psicose - r 428519 5.1.3.30 D-fructose - Dorea sp. CAG:317 D-psicose - r 428519 5.1.3.30 D-fructose the catalytic efficiency for D-psicose is 7.4fold higher than that for D-tagatose. The equilibrium ratio between D-psicose and D-fructose is 28:72. The catalytic efficiency for D-psicose is 258fold higher than that for D-tagatose [Clostridium] scindens D-psicose - r 428519 5.1.3.30 D-fructose the enzyme carries out the epimerization of D-fructose to D-psicose with a conversion yield of 32% under optimal conditions Ruminiclostridium cellulolyticum D-psicose - r 428519 5.1.3.30 D-fructose the equilibrium ratio between D-psicose and D-fructose is 28:72 at 60°C Ruminococcus sp. D-psicose - r 428519 5.1.3.30 D-fructose the equilibrium ratio between D-psicose and D-fructose is 30:70 Desmospora sp. 8437 D-psicose - r 428519 5.1.3.30 D-fructose under the optimal conditions, the equilibrium ratio of D-fructose to D-psicose is 69:31, relative activity with D-fructose is 66.4% of the activity with D-psicose Enterocloster bolteae D-psicose - r 428519 5.1.3.30 D-fructose 59.8% activity compared to the activity with D-psicose Ruminococcus sp. 5_1_39BFAA D-psicose - r 428519 5.1.3.30 D-fructose 66.4% activity compared to the activity with D-psicose Enterocloster bolteae D-psicose - r 428519 5.1.3.30 D-fructose under the optimal conditions, the equilibrium ratio of D-fructose to D-psicose is 69:31, relative activity with D-fructose is 66.4% of the activity with D-psicose Enterocloster bolteae ATCC BAA-613 D-psicose - r 428519 5.1.3.30 D-fructose 66.4% activity compared to the activity with D-psicose Enterocloster bolteae ATCC BAA-613 D-psicose - r 428519 5.1.3.30 D-fructose the enzyme carries out the epimerization of D-fructose to D-psicose with a conversion yield of 32% under optimal conditions Ruminiclostridium cellulolyticum ATCC 35319 D-psicose - r 428519 5.1.3.30 D-fructose - Ruminiclostridium cellulolyticum ATCC 35319 D-psicose - r 428519 5.1.3.30 D-fructose the catalytic efficiency for D-psicose is 7.4fold higher than that for D-tagatose. The equilibrium ratio between D-psicose and D-fructose is 28:72. The catalytic efficiency for D-psicose is 258fold higher than that for D-tagatose [Clostridium] scindens ATCC 35704 D-psicose - r 428519 5.1.3.30 D-fructose - Treponema primitia ZAS-1 D-psicose - r 428519 5.1.3.30 D-psicose - [Clostridium] scindens D-fructose - r 428532 5.1.3.30 D-psicose - Treponema primitia D-fructose - r 428532 5.1.3.30 D-psicose - Enterocloster bolteae D-fructose - r 428532 5.1.3.30 D-psicose - Ruminococcus sp. 5_1_39BFAA D-fructose - r 428532 5.1.3.30 D-psicose - Dorea sp. CAG:317 D-fructose - r 428532 5.1.3.30 D-psicose best substrate Ruminococcus sp. 5_1_39BFAA D-fructose - r 428532 5.1.3.30 D-psicose optimum substrate. Equilibrium ratio between D-psicose and D-fructose is 28:72 at 65°C Clostridium sp. D-fructose - r 428532 5.1.3.30 D-psicose the catalytic efficiency for D-psicose is 7.4fold higher than that for D-tagatose. The equilibrium ratio between D-psicose and D-fructose is 28:72. The catalytic efficiency for D-psicose is 258fold higher than that for D-tagatose [Clostridium] scindens D-fructose - r 428532 5.1.3.30 D-psicose the enzyme carries out the epimerization of D-fructose to D-psicose with a conversion yield of 32% under optimal conditions Ruminiclostridium cellulolyticum D-fructose - r 428532 5.1.3.30 D-psicose the equilibrium ratio between D-psicose and D-fructose is 28:72 at 60°C Ruminococcus sp. D-fructose - r 428532 5.1.3.30 D-psicose the equilibrium ratio between D-psicose and D-fructose is 30:70 Desmospora sp. 8437 D-fructose - r 428532 5.1.3.30 D-psicose under the optimal conditions, the equilibrium ratio of D-fructose to D-psicose is 69:31, relative activity with D-fructose is 66.4% of the activity with D-psicose Enterocloster bolteae D-fructose - r 428532 5.1.3.30 D-psicose i.e. D-allulose Dorea sp. CAG:317 D-fructose - r 428532 5.1.3.30 D-psicose i.e. D-ribo-2-hexulose or D-allulose, best substrate Enterocloster bolteae D-fructose - r 428532 5.1.3.30 D-psicose optimum substrate. Equilibrium ratio between D-psicose and D-fructose is 28:72 at 65°C Clostridium sp. BNL1100 D-fructose - r 428532 5.1.3.30 D-psicose under the optimal conditions, the equilibrium ratio of D-fructose to D-psicose is 69:31, relative activity with D-fructose is 66.4% of the activity with D-psicose Enterocloster bolteae ATCC BAA-613 D-fructose - r 428532 5.1.3.30 D-psicose - Enterocloster bolteae ATCC BAA-613 D-fructose - r 428532 5.1.3.30 D-psicose i.e. D-ribo-2-hexulose or D-allulose, best substrate Enterocloster bolteae ATCC BAA-613 D-fructose - r 428532 5.1.3.30 D-psicose the enzyme carries out the epimerization of D-fructose to D-psicose with a conversion yield of 32% under optimal conditions Ruminiclostridium cellulolyticum ATCC 35319 D-fructose - r 428532 5.1.3.30 D-psicose - [Clostridium] scindens ATCC 35704 D-fructose - r 428532 5.1.3.30 D-psicose the catalytic efficiency for D-psicose is 7.4fold higher than that for D-tagatose. The equilibrium ratio between D-psicose and D-fructose is 28:72. The catalytic efficiency for D-psicose is 258fold higher than that for D-tagatose [Clostridium] scindens ATCC 35704 D-fructose - r 428532 5.1.3.30 D-psicose - Treponema primitia ZAS-1 D-fructose - r 428532 5.1.3.30 D-psicose + NADH + H+ - Escherichia coli allitol + NAD+ - ? 437364 5.1.3.30 D-sorbose 1.2% activity compared to the activity with D-psicose Enterocloster bolteae D-tagatose - r 428537 5.1.3.30 D-sorbose 2.9% activity compared to the activity with D-psicose Ruminococcus sp. 5_1_39BFAA D-tagatose - r 428537 5.1.3.30 D-sorbose 1.2% activity compared to the activity with D-psicose Enterocloster bolteae ATCC BAA-613 D-tagatose - r 428537 5.1.3.30 D-tagatose - Enterocloster bolteae D-sorbose - r 428539 5.1.3.30 D-tagatose 23.2% activity compared to the activity with D-psicose Enterocloster bolteae D-sorbose - r 428539 5.1.3.30 D-tagatose 24.2% activity compared to the activity with D-psicose Ruminococcus sp. 5_1_39BFAA D-sorbose - r 428539 5.1.3.30 D-tagatose - Enterocloster bolteae ATCC BAA-613 D-sorbose - r 428539 5.1.3.30 D-tagatose 23.2% activity compared to the activity with D-psicose Enterocloster bolteae ATCC BAA-613 D-sorbose - r 428539 5.1.3.30 D-xylulose 3.9% activity compared to the activity with D-psicose Ruminococcus sp. 5_1_39BFAA D-ribulose - r 445432 5.1.3.30 additional information catalytic efficiency with D-tagatose is 108fold lower than cataltic efficiency with D-psicose Ruminococcus sp. ? - ? 89 5.1.3.30 additional information no activity with D-fructose 6-phosphate and D-ribulose 5-phosphate. Catalytic efficiency (kcat/KM) of D-tagatose is 127fold lower than catalytic efficiency of D-psicose Enterocloster bolteae ? - ? 89 5.1.3.30 additional information the relative activity with D-tagatose is 4.9% of the activity with D-psicose, the catalytic efficiency for D-tagatose is 249fold lower than the catalytic efficiency for D-fructose, the relative activity with D-sorbose is 0.9% of the activity wth D-psicose. No activity with D-fructose-6-phosphate or D-ribulose-5-phosphate Ruminiclostridium cellulolyticum ? - ? 89 5.1.3.30 additional information substrate specificity, overview. Poor activity with D-galactose, D-glucose, and D-arabinose, no activity with D-mannose Ruminococcus sp. 5_1_39BFAA ? - ? 89 5.1.3.30 additional information the D-psicose/D-fructose equilibrium ratio of Trpr-DPEase is 28:72. Recombinant enzyme produces D-psicose at a yield of 137.5 g/l from 500 g/l D-fructose Treponema primitia ? - ? 89 5.1.3.30 additional information the equilibrium ratio between D-fructose and D-psicose is approximately 30:70 for the recombinant enzyme, elevated temperature does not significantly shift the equilibrium toward D-psicose. The conversion ratio of D-fructose to D-psicose is calculated to be 30.1%, 31.5%, 31.8%, 31.4%, 31.1%, 31.0%, 30.4%, 29.2%, and 28.6% when the reaction temperature is 40°C, 45°C, 50°C, 55°C, 60°C, 65°C, 70°C, 75°C, and 80°C, respectively Dorea sp. CAG:317 ? - ? 89 5.1.3.30 additional information when the reaction equilibrium is reached, the ratio of D-glucose, D-fructose and D-allulose is approximately 6.5:7:3, respectively Dorea sp. CAG:317 ? - ? 89 5.1.3.30 additional information no activity with D-fructose 6-phosphate and D-ribulose 5-phosphate. Catalytic efficiency (kcat/KM) of D-tagatose is 127fold lower than catalytic efficiency of D-psicose Enterocloster bolteae ATCC BAA-613 ? - ? 89 5.1.3.30 additional information the relative activity with D-tagatose is 4.9% of the activity with D-psicose, the catalytic efficiency for D-tagatose is 249fold lower than the catalytic efficiency for D-fructose, the relative activity with D-sorbose is 0.9% of the activity wth D-psicose. No activity with D-fructose-6-phosphate or D-ribulose-5-phosphate Ruminiclostridium cellulolyticum ATCC 35319 ? - ? 89 5.1.3.30 additional information the D-psicose/D-fructose equilibrium ratio of Trpr-DPEase is 28:72. Recombinant enzyme produces D-psicose at a yield of 137.5 g/l from 500 g/l D-fructose Treponema primitia ZAS-1 ? - ? 89