4.4.1.22	5,6,7,8-tetrahydromethanopterin + formaldehyde	-	424595	
4.4.1.22	glutathione + formaldehyde	-	381969	
4.4.1.22	additional information	the enzyme glutathione-dependent formaldehyde-activating enzyme (GFA) from Paracoccus denitrificans catalyzes S-hydroxymethylglutathione (HMG) formation from glutathione and formaldehyde. Enzyme GFA binds glutathione but does not directly catalyse HMG formation under standard conditions. It is possible that GFA acts as a glutathione carrier that acts to co-localise glutathione and formaldehyde in a cellular context. NMR exchange spectroscopy is used for ligand identification. Glutathione binding structure, overview	89	
4.4.1.22	S-(hydroxymethyl)glutathione	-	371153	
4.4.1.22	S-(hydroxymethyl)glutathione	the enzyme accelerates the spontaneous reaction in which the adduct of formaldehyde and glutathione is formed, i.e. the substrate for EC 1.1.1.284, S-(hydroxymethyl)glutathione dehydrogenase, in the formaldehyde-detoxification pathway	371153