4.2.1.150	3-hydroxybutyryl-CoA	-	Clostridium acetobutylicum	crotonyl-CoA + H2O	-	?	427302	
4.2.1.150	3-hydroxybutyryl-CoA	-	Clostridium acetobutylicum	crotonyl-CoA + H2O	-	r	427302	
4.2.1.150	3-hydroxybutyryl-CoA	-	Clostridium acetobutylicum ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787	crotonyl-CoA + H2O	-	r	427302	
4.2.1.150	a short-chain trans-2-enoyl-CoA + H2O	the enzyme is specific for short chain fatty acyl-CoA substrates and is sensitive to high concentrations of crotonyl-CoA. It requires a complete coenzyme A thioester substrate for efficient catalysis	Clostridium acetobutylicum	short-chain (3S)-3-hydroxyacyl-CoA	-	?	425860	
4.2.1.150	crotonyl-CoA + H2O	-	Clostridium acetobutylicum	?	-	?	404716	
4.2.1.150	crotonyl-CoA + H2O	-	Metallosphaera sedula	(S)-3-hydroxybutanoyl-CoA	-	?	427416	
4.2.1.150	crotonyl-CoA + H2O	the enzyme is involved in autotrophic carbon fixation	Metallosphaera sedula	(S)-3-hydroxybutanoyl-CoA	-	?	427416	
4.2.1.150	crotonyl-CoA + H2O	the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway	Metallosphaera sedula	(S)-3-hydroxybutanoyl-CoA	-	?	427416	
4.2.1.150	crotonyl-CoA + H2O	bifunctional crotonyl-CoA hydratase/(S)-3-hydroxybutanoyl-CoA dehydrogenase (EC 4.2.1.150/EC 1.1.1.35)	Metallosphaera sedula	(S)-3-hydroxybutanoyl-CoA	-	?	427416	
4.2.1.150	crotonyl-CoA + H2O	-	Metallosphaera sedula DSM 5348	(S)-3-hydroxybutanoyl-CoA	-	?	427416	
4.2.1.150	crotonyl-CoA + H2O	the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway	Metallosphaera sedula DSM 5348	(S)-3-hydroxybutanoyl-CoA	-	?	427416	
4.2.1.150	crotonyl-CoA + H2O	the enzyme is involved in autotrophic carbon fixation	Metallosphaera sedula DSM 5348	(S)-3-hydroxybutanoyl-CoA	-	?	427416	
4.2.1.150	crotonyl-CoA + H2O	bifunctional crotonyl-CoA hydratase/(S)-3-hydroxybutanoyl-CoA dehydrogenase (EC 4.2.1.150/EC 1.1.1.35)	Metallosphaera sedula DSM 5348	(S)-3-hydroxybutanoyl-CoA	-	?	427416	
4.2.1.150	crotonyl-CoA + H2O	-	Clostridium aminobutyricum	3-hydroxybutyryl-CoA	-	?	427417	
4.2.1.150	crotonyl-CoA + H2O	-	Clostridium acetobutylicum	3-hydroxybutyryl-CoA	-	?	427417	
4.2.1.150	crotonyl-CoA + H2O	-	Homo sapiens	3-hydroxybutyryl-CoA	-	r	427417	
4.2.1.150	crotonyl-CoA + H2O	-	Mus musculus	3-hydroxybutyryl-CoA	-	r	427417	
4.2.1.150	crotonyl-CoA + H2O	-	Meiothermus ruber	3-hydroxybutyryl-CoA	-	r	427417	
4.2.1.150	crotonyl-CoA + H2O	-	Mus musculus C57BL/6	3-hydroxybutyryl-CoA	-	r	427417	
4.2.1.150	crotonyl-CoA + H2O	-	Meiothermus ruber DSM 1279	3-hydroxybutyryl-CoA	-	r	427417	
4.2.1.150	hexenoyl-CoA + H2O	-	Clostridium acetobutylicum	?	-	?	405423	
4.2.1.150	hexenoyl-CoA + H2O	higher analogs are not hydrated	Clostridium acetobutylicum	?	-	?	405423	
4.2.1.150	additional information	enzyme residues Ser69 and Ala24 are signature residues of CaCRT, resulting in a distinct ADP binding mode wherein the ADP moiety of acetoacetyl-CoA is bound at a different position compared with other crotonases. The substrate specificity of crotonase enzymes is determined by both the structural feature of the a3 helix region and the residues contributing the enoyl-CoA binding pocket. A tight formed a3 helix and two phenylalanine residues, Phe143 and Phe233, aid CaCRT to accommodate crotonyl-CoA as the substrate. Phe143 and Phe233 are key residues for the constitution of the crotonyl binding pocket to accommodate the four-carbon crotonyl-CoA as a substrate	Clostridium acetobutylicum	?	-	?	89	
4.2.1.150	additional information	enzyme residues Ser69 and Ala24 are signature residues of CaCRT, resulting in a distinct ADP binding mode wherein the ADP moiety of acetoacetyl-CoA is bound at a different position compared with other crotonases. The substrate specificity of crotonase enzymes is determined by both the structural feature of the a3 helix region and the residues contributing the enoyl-CoA binding pocket. A tight formed a3 helix and two phenylalanine residues, Phe143 and Phe233, aid CaCRT to accommodate crotonyl-CoA as the substrate. Phe143 and Phe233 are key residues for the constitution of the crotonyl binding pocket to accommodate the four-carbon crotonyl-CoA as a substrate	Clostridium acetobutylicum ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787	?	-	?	89