4.1.2.48 (R)-N-Cbz-alaninal + glycine conversion: 40%, glycine concentration: 70 mM, reaction temperature: 4°C, yield: 30%, L-erythro/L-threo: 16:84 Escherichia coli (2S,3R,4R)-2-amino-4-(benzyloxycarbonylamino)-3-hydroxypentanoic acid - ? 399558 4.1.2.48 (R)-N-Cbz-alaninal + glycine conversion: 60%, glycine concentration: 70 mM, reaction temperature: 4°C, yield: 30%, L-erythro/L-threo: 100:0 Streptococcus thermophilus (2S,3R,4R)-2-amino-4-(benzyloxycarbonylamino)-3-hydroxypentanoic acid - ? 399558 4.1.2.48 (R)-N-Cbz-alaninal + glycine conversion: 40%, glycine concentration: 70 mM, reaction temperature: 4°C, yield: 30%, L-erythro/L-threo: 16:84 Escherichia coli (2S,3S,4R)-2-amino-4-(benzyloxycarbonylamino)-3-hydroxypentanoic acid - ? 399559 4.1.2.48 benzyloxyacetaldehyde + glycine conversion: 45%, glycine concentration: 140 mM, reaction temperature: 25°C, yield: 30%, L-erythro/L-threo: 40:60 Escherichia coli (2S,3R)-2-amino-4-(benzyloxy)-3-hydroxybutanoic acid - ? 400539 4.1.2.48 benzyloxyacetaldehyde + glycine conversion: 68%, glycine concentration: 70 mM, reaction temperature: 4°C, yield: 40%, L-erythro/L-threo: 97:3 Streptococcus thermophilus (2S,3R)-2-amino-4-(benzyloxy)-3-hydroxybutanoic acid - ? 400539 4.1.2.48 benzyloxyacetaldehyde + glycine conversion: 45%, glycine concentration: 140 mM, reaction temperature: 25°C, yield: 30%, L-erythro/L-threo: 40:60 Escherichia coli (2S,3S)-2-amino-4-(benzyloxy)-3-hydroxybutanoic acid - ? 400540 4.1.2.48 DL-erythro-phenylserine - Escherichia coli glycine + benzaldehyde - ? 381818 4.1.2.48 DL-threo-(3-methylsulfonylphenyl)serine 121% of the activity with L-threonine Escherichia coli glycine + 3-methylsulfonylbenzaldehyde - ? 428594 4.1.2.48 DL-threo-(3-nitrophenyl)serine 143% of the activity with L-threonine Escherichia coli glycine + 3-nitrobenzaldehyde - ? 428595 4.1.2.48 DL-threo-phenylserine - Escherichia coli glycine + benzaldehyde - ? 381825 4.1.2.48 DL-threo-phenylserine 180% of the activity with L-threonine Escherichia coli glycine + benzaldehyde - ? 381825 4.1.2.48 glycine + 3,4-dihydroxybenzaldehyde - Streptomyces avermitilis L-threo-3,4-dihydroxyphenylserine - r 392113 4.1.2.48 glycine + 3,4-dihydroxybenzaldehyde L-threo-3,4-dihydroxyphenylserine synthesis activity is dramatically decreased when the condensation reaction is repeated Streptomyces coelicolor L-threo-3,4-dihydroxyphenylserine - ? 392113 4.1.2.48 glycine + 3,4-dihydroxybenzaldehyde L-threo-3,4-dihydroxyphenylserine synthesis activity is dramatically decreased when the condensation reaction is repeated Streptomyces coelicolor A3(2) L-threo-3,4-dihydroxyphenylserine - ? 392113 4.1.2.48 glycine + 3,4-dihydroxybenzaldehyde - Streptomyces coelicolor L-threo-3,4-dihydroxyphenylserine + L-erythro-3,4-dihydroxyphenylserine - r 409542 4.1.2.48 glycine + 3,4-dihydroxybenzaldehyde - Streptomyces coelicolor A3(2) L-threo-3,4-dihydroxyphenylserine + L-erythro-3,4-dihydroxyphenylserine - r 409542 4.1.2.48 glycine + acetaldehyde - Escherichia coli L-threonine - r 392115 4.1.2.48 glycine + glycolaldehyde low-specificity L-threonine aldolase is involved in a serendipitous pathway that converts 3-phosphohydroxypyruvate, an intermediate in the serine biosynthesis pathway, to L-4-phosphohydroxythreonine, an intermediate in the pyridoxal-5'-phosphate synthesis pathway in a strain of Escherichia coli that lacks 4-phosphoerythronate dehydrogenase Escherichia coli L-4-hydroxythreonine - r 415649 4.1.2.48 L-4-hydroxythreonine cleavage of L-4-hydroxythreonine is as efficient as cleavage of L-allo-threonine Escherichia coli glycine + glycolaldehyde - r 417130 4.1.2.48 L-allo-threonine - Escherichia coli glycine + acetaldehyde - ? 368182 4.1.2.48 L-allo-threonine - Escherichia coli glycine + acetaldehyde - r 368182 4.1.2.48 L-allo-threonine - Vanrija humicola glycine + acetaldehyde - r 368182 4.1.2.48 L-allo-threonine - Saccharomyces cerevisiae glycine + acetaldehyde - ? 368182 4.1.2.48 L-allo-threonine - Pseudomonas sp. glycine + acetaldehyde - r 368182 4.1.2.48 L-allo-threonine - Caulobacter vibrioides glycine + acetaldehyde - ? 368182 4.1.2.48 L-allo-threonine 291% of the activity with L-threonine Escherichia coli glycine + acetaldehyde - ? 368182 4.1.2.48 L-allo-threonine - Caulobacter vibrioides CB15 glycine + acetaldehyde - ? 368182 4.1.2.48 L-allo-threonine - Escherichia coli WV_060327 glycine + acetaldehyde - ? 368182 4.1.2.48 L-allo-threonine - Pseudomonas sp. NCIMB 10558 glycine + acetaldehyde - r 368182 4.1.2.48 L-erythro-phenylserine - Pseudomonas sp. glycine + benzaldehyde - ? 417140 4.1.2.48 L-erythro-phenylserine - Pseudomonas sp. NCIMB 10558 glycine + benzaldehyde - ? 417140 4.1.2.48 L-phenylserine - Streptomyces coelicolor ? - ? 392404 4.1.2.48 L-phenylserine - Streptomyces coelicolor A3(2) ? - ? 392404 4.1.2.48 L-serine - Vanrija humicola glycine + formaldehyde - ? 417181 4.1.2.48 L-Thr - Streptomyces coelicolor glycine + acetaldehyde - ? 1822 4.1.2.48 L-Thr - Streptomyces coelicolor A3(2) glycine + acetaldehyde - ? 1822 4.1.2.48 L-threo-3,4-dihydroxyphenylserine - Streptomyces coelicolor glycine + 3,4-dihydroxybenzaldehyde - ? 392436 4.1.2.48 L-threo-3,4-dihydroxyphenylserine - Streptomyces coelicolor A3(2) glycine + 3,4-dihydroxybenzaldehyde - ? 392436 4.1.2.48 L-threo-beta-3,4-dihydroxyphenylserine - Pseudomonas sp. glycine + 3,4-dihydroxybenzaldehyde - ? 417188 4.1.2.48 L-threo-beta-3,4-dihydroxyphenylserine - Pseudomonas sp. NCIMB 10558 glycine + 3,4-dihydroxybenzaldehyde - ? 417188 4.1.2.48 L-threo-beta-3,4-methylenedioxyphenylserine - Pseudomonas sp. ? - ? 417189 4.1.2.48 L-threo-beta-3,4-methylenedioxyphenylserine - Pseudomonas sp. NCIMB 10558 ? - ? 417189 4.1.2.48 L-threo-phenylserine - Pseudomonas sp. glycine + benzaldehyde - ? 417190 4.1.2.48 L-threonine - Escherichia coli glycine + acetaldehyde - ? 289621 4.1.2.48 L-threonine - Escherichia coli glycine + acetaldehyde - r 289621 4.1.2.48 L-threonine - Vanrija humicola glycine + acetaldehyde - r 289621 4.1.2.48 L-threonine - Candida albicans glycine + acetaldehyde - r 289621 4.1.2.48 L-threonine - Saccharomyces cerevisiae glycine + acetaldehyde - r 289621 4.1.2.48 L-threonine - Pseudomonas sp. glycine + acetaldehyde - r 289621 4.1.2.48 L-threonine - Caulobacter vibrioides glycine + acetaldehyde - ? 289621 4.1.2.48 L-threonine - Caulobacter vibrioides CB15 glycine + acetaldehyde - ? 289621 4.1.2.48 L-threonine - Candida albicans SGY269 glycine + acetaldehyde - r 289621 4.1.2.48 L-threonine - Escherichia coli WV_060327 glycine + acetaldehyde - ? 289621 4.1.2.48 L-threonine - Pseudomonas sp. NCIMB 10558 glycine + acetaldehyde - r 289621 4.1.2.48 additional information glycine metabolism Candida albicans ? - ? 89 4.1.2.48 additional information by manipulating reaction parameters, SHMT yields exclusively L-erythro diastereomers in 34-60% conversion. SHMT is among the most stereoselective L-threonine aldolases described. This is due to its activity-temperature dependence: at 4°C SHMT has high synthetic activity but negligible retro-aldol activity on l-threonine. Thus, the kinetic l-erythro isomer is largely favored and the reactions are virtually irreversible, highly stereoselective, and in turn, give excellent conversion Streptococcus thermophilus ? - ? 89 4.1.2.48 additional information glycine metabolism Candida albicans SGY269 ? - ? 89 4.1.2.48 N-(S)-benzyloxycarbonyl-alaninal + glycine conversion: 48%, glycine concentration: 280 mM, reaction temperature: 4°C, yield: 30%, L-erythro/L-threo: 100:0 Streptococcus thermophilus (2S,3R,4S)-2-amino-4-(benzyloxycarbonylamino)-3-hydroxypentanoic acid - ? 401429 4.1.2.48 N-(S)-benzyloxycarbonyl-alaninal + glycine conversion: 54%, glycine concentration: 140 mM, reaction temperature: 25°C, yield: 27%, L-erythro/L-threo: 18:82 Escherichia coli (2S,3R,4S)-2-amino-4-(benzyloxycarbonylamino)-3-hydroxypentanoic acid - ? 401429 4.1.2.48 N-(S)-benzyloxycarbonyl-alaninal + glycine conversion: 54%, glycine concentration: 140 mM, reaction temperature: 25°C, yield: 27%, L-erythro/L-threo: 18:82 Escherichia coli (2S,3S,4S)-2-amino-4-(benzyloxycarbonylamino)-3-hydroxypentanoic acid - ? 401430 4.1.2.48 N-benzyloxycarbonyl-3-aminopropanal + glycine conversion: 34%, glycine concentration: 70 mM, reaction temperature: 25°C, yield: 10%, L-erythro/L-threo: 50:50 Streptococcus thermophilus (2S,3R)-2-amino-5-(benzyloxycarbonylamino)-3-hydroxypentanoic acid - ? 401447 4.1.2.48 N-benzyloxycarbonyl-3-aminopropanal + glycine conversion: 49%, glycine concentration: 70 mM, reaction temperature: 25°C, yield: 11%, L-erythro/L-threo: 50:50 Escherichia coli (2S,3R)-2-amino-5-(benzyloxycarbonylamino)-3-hydroxypentanoic acid - ? 401447 4.1.2.48 N-benzyloxycarbonyl-3-aminopropanal + glycine conversion: 20%, glycine concentration: 70 mM, reaction temperature: 4°C, yield: 3%, L-erythro/L-threo: 100:0 Streptococcus thermophilus (2S,3S)-2-amino-5-(benzyloxycarbonylamino)-3-hydroxypentanoic acid - ? 401448 4.1.2.48 N-benzyloxycarbonyl-3-aminopropanal + glycine conversion: 49%, glycine concentration: 70 mM, reaction temperature: 25°C, yield: 11%, L-erythro/L-threo: 50:50 Escherichia coli (2S,3S)-2-amino-5-(benzyloxycarbonylamino)-3-hydroxypentanoic acid - ? 401448 4.1.2.48 N-benzyloxycarbonyl-glycinal + glycine conversion: 35%, glycine concentration: 70 mM, reaction temperature: 4°C, yield: 13%, L-erythro/L-threo: 86:14 Streptococcus thermophilus (2S,3R)-2-amino-4-(benzyloxycarbonylamino)-3-hydroxybutanoic acid - ? 401449 4.1.2.48 N-benzyloxycarbonyl-glycinal + glycine conversion: 60%, glycine concentration: 140 mM, reaction temperature: 25°C, yield: 18%, L-erythro/L-threo: 30:70 Escherichia coli (2S,3R)-2-amino-4-(benzyloxycarbonylamino)-3-hydroxybutanoic acid - ? 401449 4.1.2.48 N-benzyloxycarbonyl-glycinal + glycine conversion: 35%, glycine concentration: 70 mM, reaction temperature: 4°C, yield: 13%, L-erythro/L-threo: 86:14 Streptococcus thermophilus (2S,3S)-2-amino-4-(benzyloxycarbonylamino)-3-hydroxybutanoic acid - ? 401450 4.1.2.48 N-benzyloxycarbonyl-glycinal + glycine conversion: 60%, glycine concentration: 140 mM, reaction temperature: 25°C, yield: 18%, L-erythro/L-threo: 30:70 Escherichia coli (2S,3S)-2-amino-4-(benzyloxycarbonylamino)-3-hydroxybutanoic acid - ? 401450