3.7.1.4 2',3,4',6'-tetrahydroxy-4-methoxydihydrochalcone + H2O - Aspergillus niger phloroglucinol + ? - ? 190225 3.7.1.4 2',3,4',6'-tetrahydroxy-dihydrochalcone + H2O - Aspergillus niger phloroglucinol + ? - ? 190226 3.7.1.4 2',3,4,4',6'-pentahydroxy-dihydrochalcone 2'-beta-D-glucoside + H2O - Aspergillus niger phloroglucinol beta-D-glucoside + 3-(3,4-dihydroxyphenyl)propanoic acid - ? 190230 3.7.1.4 2',4'-dihydroxy-4-methoxydihydrochalcone + H2O - Aspergillus niger resorcinol + ? - ? 190228 3.7.1.4 2',4'-dihydroxypropiophenone + H2O - Aspergillus niger resorcinol - ? 190231 3.7.1.4 2',4,4',6'-tetrahydroxy-dihydrochalcone + H2O - Aspergillus niger phloroglucinol + ? - ? 190227 3.7.1.4 2',4,4'-trihydroxyhydrochalcone - Aspergillus niger ? - ? 190224 3.7.1.4 2',6'-dihydroxy-4,4'-dimethoxydihydrochalcone + H2O - Aspergillus niger 3-methoxyresorcinol + ? - ? 190229 3.7.1.4 3'-methylchloracetophenone + H2O - Aspergillus niger ? - ? 190222 3.7.1.4 baohuoside I + H2O - Rattus norvegicus ? - ? 432712 3.7.1.4 baohuoside I + H2O - Rattus norvegicus Sprague-Dawley ? - ? 432712 3.7.1.4 beta-methyl D-glucoside + H2O - Platyrrhini ? - ? 190220 3.7.1.4 cellobiose + H2O - Platyrrhini 2 D-glucose - ? 94496 3.7.1.4 cerebrosides + H2O - Platyrrhini ? - ? 190213 3.7.1.4 chloracetophenone + H2O - Aspergillus niger ? - ? 190223 3.7.1.4 dihydrochalcone phloretin + H2O - Eubacterium ramulus phloroglucinol + phloretic acid - ? 446235 3.7.1.4 epimedin A + H2O - Rattus norvegicus ? - ? 432789 3.7.1.4 epimedin A + H2O - Rattus norvegicus Sprague-Dawley ? - ? 432789 3.7.1.4 epimedin B + H2O - Rattus norvegicus ? - ? 432790 3.7.1.4 epimedin B + H2O - Rattus norvegicus Sprague-Dawley ? - ? 432790 3.7.1.4 epimedin C + H2O - Rattus norvegicus ? - ? 432791 3.7.1.4 icariin + H2O - Rattus norvegicus ? - ? 432820 3.7.1.4 icariin + H2O the metabolic pathways of icariin by rat intestinal flora and enzyme solution are basically the same, including 3-O-rhamnose, 7-O-glucose hydrolysis or dual 3, 7-hydrolysis. Compared to 3-O-rhamnose and dual hydrolysis of 3, 7-, the 7-O-glucose hydrolysis is easier. The yielded metabolites contain M1 (icariside I), M2 (icaritin), and baohuoside I Rattus norvegicus ? - ? 432820 3.7.1.4 lactose + H2O 66% reduced activity towards the substrate in rats fed a low starch diet as compared to rats fed a high starch diet Rattus norvegicus ? - ? 94580 3.7.1.4 lactose + H2O - Homo sapiens D-glucose + D-galactose - ? 115615 3.7.1.4 lactose + H2O - Homo sapiens D-galactose + D-glucose - ? 190211 3.7.1.4 lactose + H2O - Rattus norvegicus D-galactose + D-glucose - ? 190211 3.7.1.4 lactose + H2O - Platyrrhini D-galactose + D-glucose - ? 190211 3.7.1.4 lactose + H2O - Homo sapiens beta-D-galactose + beta-D-glucose - ? 373672 3.7.1.4 lactose + H2O - Rattus norvegicus beta-D-galactose + beta-D-glucose - ? 373672 3.7.1.4 lactose + H2O - Homo sapiens glucose + galactose - ? 389079 3.7.1.4 monoacetylphloroglucinol + H2O - Mycobacteroides abscessus ? - ? 446308 3.7.1.4 monoacetylphloroglucinol + H2O - Mycobacteroides abscessus 103 ? - ? 446308 3.7.1.4 additional information no activity with phloretin-2'-glucoside, i.e. phloridzin, neohesperidin dihydrochalcone, 1,3-diphenyl-1,3-propandione, and trans-1,3-diphenyl-2,3-epoxy-propan-1-one Eubacterium ramulus ? - ? 89 3.7.1.4 additional information the enzyme interacts with ER chaperones, immunglobulin binding protein, and calnexin at non-permissive temperatures in the ER Homo sapiens ? - ? 89 3.7.1.4 additional information the enzyme preparation also shows maltase-glucoamylase activity, EC 3.2.1.20, at 3.8 U/mg Rattus norvegicus ? - ? 89 3.7.1.4 additional information hydrolysis of prenylated flavonoids from Epimedium sp., Epimedii herba, used for a tranditional chinese medicine tonic, Yinyanghuo. Comparison of hydrolysis rates and substrate specificities of intestinal flora and intestinal enzymes, especially lactase phlorizin hydrolase, with the flavonoid substrates, overview. Flavonoid metabolic rates with rat intestinal enzyme are higher than those with intestinal flora. The intestinal hydrolysis of glycosides by intestinal enzymes is rapid. Even icariin is completely metabolized in 6 h and the epimedin A is totally metabolized in 12 h in incubation with intestinal enzyme Rattus norvegicus ? - ? 89 3.7.1.4 additional information no activity with 1,3-diphenyl 1,3-propanedione and diacetylphloroglucinol, a substrate of diacetyl phloroglucinol hydrolase, Phlg Eubacterium ramulus ? - ? 89 3.7.1.4 additional information no activity with glycosides phloretin-2'-glucoside (phloridzin) and neohesperidin dihydrochalcone, nor with compounds showing a structure similar to phloretin, such as 1,3-diphenyl-1,3-propandione and trans-1,3-diphenyl-2,3-epoxy-propan-1-one Eubacterium ramulus ? - ? 89 3.7.1.4 additional information the enzyme has two distinct catalytic active sites, one for the hydrolysis of lactose and flavonoid glucosides and another, phlorizin hydrolase, for the hydrolysis of phlorizin and phlorizin-glucosylceramides Rattus norvegicus ? - ? 89 3.7.1.4 additional information hydrolysis of prenylated flavonoids from Epimedium sp., Epimedii herba, used for a tranditional chinese medicine tonic, Yinyanghuo. Comparison of hydrolysis rates and substrate specificities of intestinal flora and intestinal enzymes, especially lactase phlorizin hydrolase, with the flavonoid substrates, overview. Flavonoid metabolic rates with rat intestinal enzyme are higher than those with intestinal flora. The intestinal hydrolysis of glycosides by intestinal enzymes is rapid. Even icariin is completely metabolized in 6 h and the epimedin A is totally metabolized in 12 h in incubation with intestinal enzyme Rattus norvegicus Sprague-Dawley ? - ? 89 3.7.1.4 additional information the enzyme has two distinct catalytic active sites, one for the hydrolysis of lactose and flavonoid glucosides and another, phlorizin hydrolase, for the hydrolysis of phlorizin and phlorizin-glucosylceramides Rattus norvegicus Sprague-Dawley ? - ? 89 3.7.1.4 additional information no activity with phloretin-2'-glucoside, i.e. phloridzin, neohesperidin dihydrochalcone, 1,3-diphenyl-1,3-propandione, and trans-1,3-diphenyl-2,3-epoxy-propan-1-one Eubacterium ramulus wK1 ? - ? 89 3.7.1.4 additional information no activity with glycosides phloretin-2'-glucoside (phloridzin) and neohesperidin dihydrochalcone, nor with compounds showing a structure similar to phloretin, such as 1,3-diphenyl-1,3-propandione and trans-1,3-diphenyl-2,3-epoxy-propan-1-one Eubacterium ramulus wK1 ? - ? 89 3.7.1.4 N-palmitoyldihydroglucosylceramide + H2O - Platyrrhini ? - ? 190214 3.7.1.4 o-nitrophenyl-beta-D-galactoside + H2O - Platyrrhini o-nitrophenol + beta-D-galactose - ? 94288 3.7.1.4 o-nitrophenyl-beta-D-glucoside + H2O - Platyrrhini o-nitrophenol + beta-D-glucose - ? 94285 3.7.1.4 p-nitrophenyl-beta-D-galactoside + H2O - Platyrrhini p-nitrophenol + beta-D-galactose - ? 94135 3.7.1.4 p-nitrophenyl-beta-D-glucoside + H2O - Platyrrhini p-nitrophenol + beta-D-glucose - ? 94136 3.7.1.4 phloretin + H2O - Homo sapiens phloretate + phloroglucinol - ? 190210 3.7.1.4 phloretin + H2O - Rattus norvegicus phloretate + phloroglucinol - ? 190210 3.7.1.4 phloretin + H2O - Aspergillus niger phloretate + phloroglucinol - ? 190210 3.7.1.4 phloretin + H2O - Pantoea agglomerans phloretate + phloroglucinol - ? 190210 3.7.1.4 phloretin + H2O - Platyrrhini phloretate + phloroglucinol - ? 190210 3.7.1.4 phloretin + H2O - Eubacterium ramulus phloretate + phloroglucinol - ? 190210 3.7.1.4 phloretin + H2O a C-C bond cleaving enzyme Eubacterium ramulus phloretate + phloroglucinol - ? 190210 3.7.1.4 phloretin + H2O phloretin hydrolase catalyzes the hydrolysis of the dihydrochalcone phloretin to phloroglucinol and phloretic acid, performing a formal retro-Friedel-Crafts acylation reaction on its substrate, it is a C-C bond cleaving enzyme Eubacterium ramulus phloretate + phloroglucinol - ? 190210 3.7.1.4 phloretin + H2O - Eubacterium ramulus wK1 phloretate + phloroglucinol - ? 190210 3.7.1.4 phloretin + H2O a C-C bond cleaving enzyme Eubacterium ramulus wK1 phloretate + phloroglucinol - ? 190210 3.7.1.4 phloretin + H2O - Pantoea agglomerans Y46 phloretate + phloroglucinol - ? 190210 3.7.1.4 phloretin + H2O - Mycobacteroides abscessus phloroglucinol + 3-(4-hydroxyphenyl)propionic acid - ? 371093 3.7.1.4 phloretin + H2O during flavonoid degradation Eubacterium ramulus phloroglucinol + 3-(4-hydroxyphenyl)propionic acid - ? 371093 3.7.1.4 phloretin + H2O - Mycobacteroides abscessus 103 phloroglucinol + 3-(4-hydroxyphenyl)propionic acid - ? 371093 3.7.1.4 phlorhizin + H2O - Homo sapiens 3-(4-hydroxyphenyl)propanoate + arbutin - ? 190212 3.7.1.4 phlorhizin + H2O - Rattus norvegicus 3-(4-hydroxyphenyl)propanoate + arbutin - ? 190212 3.7.1.4 phlorhizin + H2O - Aspergillus niger 3-(4-hydroxyphenyl)propanoate + arbutin - ? 190212 3.7.1.4 phlorhizin + H2O - Platyrrhini 3-(4-hydroxyphenyl)propanoate + arbutin - ? 190212 3.7.1.4 pyridoxine-5'-beta-D-glucoside + H2O - Rattus norvegicus pyridoxine + D-glucose - ? 371136 3.7.1.4 pyridoxine-5'-beta-D-glucoside + H2O enzyme might play an important role in providing the bioavailability of pyridoxine-5'-beta-D-glucoside Rattus norvegicus pyridoxine + D-glucose - ? 371136 3.7.1.4 pyridoxine-5'-beta-D-glucoside + lactose - Rattus norvegicus pyridoxine + beta-D-glucose + pyridoxine disaccharide product identification, product is formed by a pyridoxine molecule with the 5'-beta-gkucoside and another glucose or galactose moiety ? 374487 3.7.1.4 salicin + H2O - Platyrrhini ? - ? 13703 3.7.1.4 sucrose + H2O 87% reduced activity towards the substrate in rats fed a low starch diet as compared to rats fed a high starch diet Rattus norvegicus D-fructose + D-glucose - ? 94154 3.7.1.4 phloretin + H2O - Eubacterium ramulus phloroglucinol + 3-(4-hydroxyphenyl)propionic acid - ir 371093 3.7.1.4 phloretin + H2O - Eubacterium ramulus wK1 phloroglucinol + 3-(4-hydroxyphenyl)propionic acid - ir 371093