3.5.5.7 (E)-2-methyl-2-butenenitrile + H2O regioselective hydrolysis, no detectable conversion of (Z)-2-methyl-2-butenenitrile Acidovorax facilis (E)-2-methyl-2-butenoic acid + NH3 - ? 383466 3.5.5.7 (E)-2-methyl-2-butenenitrile + H2O regioselective hydrolysis, no detectable conversion of (Z)-2-methyl-2-butenenitrile Acidovorax facilis 72W (E)-2-methyl-2-butenoic acid + NH3 - ? 383466 3.5.5.7 1,4-dicyano-2-butene + H2O 42.6% of the activity with crotononitrile Rhodococcus rhodochrous ? - ? 18130 3.5.5.7 1,4-dicyanobutane + H2O 114.3% activity compared to 3-cyanopyridine Cereibacter sphaeroides ? - ? 433076 3.5.5.7 1,8-dicyanooctane + H2O 114.3% activity compared to 3-cyanopyridine Cereibacter sphaeroides ? - ? 433081 3.5.5.7 1-cyclohexeneacetonitrile + H2O 18.2% of the activity with crotononitrile Rhodococcus rhodochrous 1-cyclohexene carboxylate + NH3 - ? 18170 3.5.5.7 1-cyclopenteneacetonitrile + H2O 24.9% of the activity with crotononitrile Rhodococcus rhodochrous 1-cyclopentene carboxylate + NH3 - ? 18169 3.5.5.7 2 fumarodinitrile + 3 H2O 11% compared to the activity with 3-hexenedinitrile. Fumarodinitrile is converted to the monocarboxylate and the monocarboxamide in a ratio of about 65:35 Pseudomonas fluorescens 3-cyanoacrylamide + 3-cyanoacrylic acid + NH3 - ? 454815 3.5.5.7 2,4-dicyano-1-butene + H2O 127% of the activity with crotononitrile Rhodococcus rhodochrous ? - ? 18131 3.5.5.7 2,6-dichlorobenzonitrile + H2O - Pseudomonas fluorescens 2,6-dichlorobenzamide + ? - ? 395399 3.5.5.7 2,6-dichlorobenzonitrile + H2O - Pseudomonas putida 2,6-dichlorobenzamide + ? - ? 395399 3.5.5.7 2,6-dichlorobenzonitrile + H2O - Rhizobium sp. 2,6-dichlorobenzamide + ? - ? 395399 3.5.5.7 2,6-dichlorobenzonitrile + H2O - Pseudomonas putida 11388 2,6-dichlorobenzamide + ? - ? 395399 3.5.5.7 2,6-dichlorobenzonitrile + H2O - Pseudomonas fluorescens 11387 2,6-dichlorobenzamide + ? - ? 395399 3.5.5.7 2,6-dichlorobenzonitrile + H2O - Rhizobium sp. 11401 2,6-dichlorobenzamide + ? - ? 395399 3.5.5.7 2-aminocrotononitrile + H2O 30.3% of the activity with crotononitrile Rhodococcus rhodochrous 2-aminocrotonic acid + NH3 - ? 18122 3.5.5.7 2-aminocrotononitrile + H2O 30.3% of the activity with crotononitrile Rhodococcus rhodochrous K22 2-aminocrotonic acid + NH3 - ? 18122 3.5.5.7 2-butenenitrile + H2O - Synechocystis sp. 2-butenoic acid + NH3 - ? 395489 3.5.5.7 2-butenenitrile + H2O worst substrate Synechocystis sp. 2-butenic acid + NH3 - ? 433184 3.5.5.7 2-chloroacrylonitrile + H2O 47.1% of the activity with crotononitrile Rhodococcus rhodochrous 2-chloroacrylic acid + NH3 - ? 18123 3.5.5.7 2-chloropropionitrile + 2 H2O 86% compared to the activity with 3-hexenedinitrile Pseudomonas fluorescens 2-chloropropionic acid + NH3 - ? 454917 3.5.5.7 2-cyanopyridine + 2 H2O - Rhodococcus rhodochrous 2-pyridine carboxylic acid + NH3 - ? 141125 3.5.5.7 2-cyanopyridine + 2 H2O low activity Meyerozyma guilliermondii pyridine 2-carboxylic acid + NH3 - ? 371654 3.5.5.7 2-cyanopyridine + 2 H2O second best substrate Synechocystis sp. pyridine 2-carboxylic acid + NH3 - ? 371654 3.5.5.7 2-cyanopyridine + 2 H2O 110% activity compared to benzonitrile Fusarium proliferatum pyridine 2-carboxylic acid + NH3 - ? 371654 3.5.5.7 2-cyanopyridine + 2 H2O activity is 3.35fold higher compared to activity with 3-cyanopyridine Cupriavidus necator pyridine 2-carboxylic acid + NH3 - ? 371654 3.5.5.7 2-furonitrile + H2O 52.0% of the activity with crotononitrile Rhodococcus rhodochrous furoic acid + NH3 - ? 18163 3.5.5.7 2-hydroxy-3-butenenitrile + 2 H2O 8% compared to the activity with 3-hexenedinitrile Pseudomonas fluorescens 2-hydroxybut-3-enoic acid + NH3 - ? 454926 3.5.5.7 2-hydroxybutyronitrile + H2O 3% compared to the activity with 3-hexenedinitrile Pseudomonas fluorescens 2-hydroxybutyrate + NH3 - ? 454929 3.5.5.7 2-methyl-2-butenenitrile + H2O 14.9% of the activity with crotononitrile Rhodococcus rhodochrous 2-methyl-2-butenoic acid + NH3 - ? 18126 3.5.5.7 2-methyl-3-butenenitrile + 2 H2O 3% compared to the activity with 3-hexenedinitrile Pseudomonas fluorescens 2-methyl-but-3-enoic acid + NH3 - ? 454932 3.5.5.7 2-methyl-3-butenenitrile + H2O 46.8% of the activity with crotononitrile Rhodococcus rhodochrous 2-methyl-3-butenoic acid + NH3 - ? 18127 3.5.5.7 2-methyleneglutarodinitrile + H2O 11% compared to the activity with 3-hexenedinitrile. 2-Methyleneglutarodinitrile is mainly converted to the corresponding monocarboxylic acid , but also some traces of the monoamide are formed Pseudomonas fluorescens ? + NH3 - ? 454936 3.5.5.7 2-methylglutaronitrile + 2 H2O 77% activity compared to glutaronitrile uncultured bacterium 4-cyanopentanoic acid + NH3 - ? 395580 3.5.5.7 2-methylglutaronitrile + 2 H2O activity is 10% compared to activity with 3-cyanopyridine Cupriavidus necator 4-cyanopentanoic acid + NH3 - ? 395580 3.5.5.7 2-methylglutaronitrile + 4 H2O - Streptomyces sp. 2-methylglutaric acid + 2 NH3 - ? 395579 3.5.5.7 2-methylglutaronitrile + 4 H2O activity is 2.3fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis 2-methylglutaric acid + 2 NH3 - ? 395579 3.5.5.7 2-methylglutaronitrile + 4 H2O activity is 2.3fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis Q4.6 2-methylglutaric acid + 2 NH3 - ? 395579 3.5.5.7 2-methylglutaronitrile + 4 H2O 23.3% activity compared to 3-cyanopyridine Cereibacter sphaeroides 2-methylglutarate + 2 NH3 - ? 433209 3.5.5.7 2-pentenenitrile + H2O 6.33% of the activity with crotononitrile Rhodococcus rhodochrous 2-pentenoic acid + NH3 - ? 18128 3.5.5.7 2-phenylbutyronitrile + 2 H2O activity is 11% compared to activity with 3-cyanopyridine Cupriavidus necator 2-phenylpropionic acid + NH3 - ? 454940 3.5.5.7 2-phenylpropionitrile + 2 H2O - Pseudomonas fluorescens 2-phenylpropionic acid + NH3 - ? 383784 3.5.5.7 2-thiopheneacetonitrile + H2O 73.5% of the activity with crotononitrile Rhodococcus rhodochrous thiophene-2-carboxylate + NH3 - ? 18174 3.5.5.7 2-thiophenecarbonitrile + H2O 61.1% of the activity with crotononitrile Rhodococcus rhodochrous 2-thiophenecarboxylate + NH3 - ? 18164 3.5.5.7 3,3'-oxydipropionitrile + H2O 29.0% of the activity with crotononitrile Rhodococcus rhodochrous ? + NH3 - ? 18152 3.5.5.7 3-aminobutyronitrile + H2O 201% activity compared to benzonitrile Fusarium proliferatum 3-aminobutyric acid + NH3 - ? 433242 3.5.5.7 3-aminopropionitrile + 2 H2O the enzyme catalyzes the hydrolysis of 3-aminopropionitrile at high substrate concentration up to 3 M. With the increase of substrate concentration, 3-aminopropanamide is formed, reaching 33% at the substrate concentration of 3 M Bradyrhizobium japonicum beta-alanine + NH3 - ? 454989 3.5.5.7 3-aminopropionitrile + 2 H2O the enzyme catalyzes the hydrolysis of 3-aminopropionitrile at high substrate concentration up to 3 M. With the increase of substrate concentration, 3-aminopropanamide is formed, reaching 33% at the substrate concentration of 3 M Bradyrhizobium japonicum USDA110 beta-alanine + NH3 - ? 454989 3.5.5.7 3-butenenitrile + H2O - Synechocystis sp. 3-butenoic acid + NH3 - ? 395705 3.5.5.7 3-chloropropionitrile + H2O 113% of the activity with crotononitrile Rhodococcus rhodochrous 3-chloropropanoate + NH3 - ? 18141 3.5.5.7 3-cyanopyridine + 2 H2O - Rhodococcus rhodochrous nicotinic acid + NH3 - ? 141122 3.5.5.7 3-cyanopyridine + 2 H2O - Rhodococcus rhodochrous J1 nicotinic acid + NH3 - ? 141122 3.5.5.7 3-cyanopyridine + 2 H2O - Meyerozyma guilliermondii pyridine 3-carboxylic acid + NH3 - ? 371827 3.5.5.7 3-cyanopyridine + 2 H2O - Cupriavidus necator pyridine 3-carboxylic acid + NH3 - ? 371827 3.5.5.7 3-cyanopyridine + 2 H2O - Synechocystis sp. pyridine 3-carboxylic acid + NH3 - ? 371827 3.5.5.7 3-cyanopyridine + 2 H2O - Thermotoga maritima pyridine 3-carboxylic acid + NH3 - ? 371827 3.5.5.7 3-cyanopyridine + 2 H2O - Pannonibacter carbonis pyridine 3-carboxylic acid + NH3 - ? 371827 3.5.5.7 3-cyanopyridine + 2 H2O 100% activity Cereibacter sphaeroides pyridine 3-carboxylic acid + NH3 - ? 371827 3.5.5.7 3-cyanopyridine + 2 H2O 151% activity compared to benzonitrile Fusarium proliferatum pyridine 3-carboxylic acid + NH3 - ? 371827 3.5.5.7 3-cyanopyridine + 2 H2O 100% activity Cereibacter sphaeroides LHS-305 pyridine 3-carboxylic acid + NH3 - ? 371827 3.5.5.7 3-cyanopyridine + 2 H2O - Pannonibacter carbonis Q4.6 pyridine 3-carboxylic acid + NH3 - ? 371827 3.5.5.7 3-cyanopyridine + H2O 7.72% of the activity with crotononitrile Rhodococcus rhodochrous 3-carboxypyridine + NH3 - ? 18160 3.5.5.7 3-cyanopyridine + H2O 11% activity compared to glutaronitrile uncultured bacterium 3-carboxypyridine + NH3 - ? 18160 3.5.5.7 3-ethoxyacrylonitrile + H2O 19.1% of the activity with crotononitrile Rhodococcus rhodochrous 3-ethoxyacrylic acid + NH3 - ? 18121 3.5.5.7 3-ethoxyacrylonitrile + H2O 19.1% of the activity with crotononitrile Rhodococcus rhodochrous K22 3-ethoxyacrylic acid + NH3 - ? 18121 3.5.5.7 3-ethoxybenzonitrile + H2O 21.1% of the activity with crotononitrile Rhodococcus rhodochrous 3-ethoxybenzoate + NH3 - ? 18159 3.5.5.7 3-hexenedinitrile + H2O - Pseudomonas fluorescens ? + NH3 - ? 455006 3.5.5.7 3-hydroxyglutaronitrile + 4 H2O activity is 38% compared to activity with 3-cyanopyridine Cupriavidus necator 3-hydroxyglutaric acid + 2 NH3 - ? 433250 3.5.5.7 3-hydroxypropionitrile + 2 H2O activity is 1.5fold higher compared to activity with 3-cyanopyridine Cupriavidus necator 3-hydroxypropionitrile + NH3 - ? 455009 3.5.5.7 3-methoxybenzonitrile 24.8% of the activity with crotononitrile Rhodococcus rhodochrous 3-methoxybenzoate + NH3 - ? 18158 3.5.5.7 3-nitrobenzonitrile + H2O 74.5% of the activity with crotononitrile Rhodococcus rhodochrous 3-nitrobenzoate + NH3 - ? 18156 3.5.5.7 3-nitrobenzonitrile + H2O 110% activity compared to benzonitrile Fusarium proliferatum 3-nitrobenzoic acid + NH3 - ? 141133 3.5.5.7 3-pentenenitrile + H2O 132% of the activity with crotononitrile Rhodococcus rhodochrous 3-pentenoic acid + NH3 - ? 18129 3.5.5.7 3-phenylpropionitrile + 2 H2O activity is 2.15fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis 3-phenylpropionic acid + NH3 - ? 455018 3.5.5.7 3-phenylpropionitrile + 2 H2O activity is 61% compared to activity with 3-cyanopyridine Cupriavidus necator 3-phenylpropionic acid + NH3 - ? 455018 3.5.5.7 3-phenylpropionitrile + H2O 151% activity compared to benzonitrile Fusarium proliferatum 3-phenylpropionic acid + NH3 - ? 18109 3.5.5.7 3-phenylpropionitrile + H2O 151% activity compared to benzonitrile Fusarium proliferatum AUF-2 3-phenylpropionic acid + NH3 - ? 18109 3.5.5.7 3-phenylpropionitrile + H2O 59% activity compared to glutaronitrile uncultured bacterium ? - ? 414062 3.5.5.7 3-phenylpropionitrile + H2O 147.3% activity compared to 3-cyanopyridine Cereibacter sphaeroides 3-phenylpropanoate + NH3 - ? 433263 3.5.5.7 3-thiopheneacetonitrile + H2O 66.3% of the activity with crotononitrile Rhodococcus rhodochrous thiophene-3-carboxylate + NH3 - ? 18175 3.5.5.7 3-tolunitrile + H2O 67.9% of the activity with crotononitrile Rhodococcus rhodochrous 3-methylbenzenecarboxylic acid + NH3 - ? 18155 3.5.5.7 3-xylylene dicyanide + H2O 14.62% of the activity with crotononitrile Rhodococcus rhodochrous ? - ? 18173 3.5.5.7 4-aminobenzonitrile + 2 H2O 57.6% compared to the activity with 3-cyanopyridine Thermotoga maritima 4-aminobenzoic acid + NH3 - ? 420027 3.5.5.7 4-aminobutyronitrile + H2O 251% activity compared to benzonitrile Fusarium proliferatum 4-aminobutyric acid + NH3 - ? 433272 3.5.5.7 4-chlorobutyronitrile + 2 H2O 66.7% compared to the activity with 3-cyanopyridine Thermotoga maritima 4-chlorobutanoic acid + NH3 - ? 455035 3.5.5.7 4-chlorobutyronitrile + 2 H2O activity is 2.44fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis 4-chlorobutanoic acid + NH3 - ? 455035 3.5.5.7 4-chlorobutyronitrile + 2 H2O activity is 1.49fold higher compared to activity with 3-cyanopyridine Cupriavidus necator 4-chlorobutyric acid + NH3 - ? 455036 3.5.5.7 4-chlorobutyronitrile + H2O 116% of the activity with crotononitrile Rhodococcus rhodochrous 4-chlorobutyrate + NH3 - ? 18142 3.5.5.7 4-chlorobutyronitrile + H2O 135% activity compared to 3-cyanopyridine Cereibacter sphaeroides 4-chlorobutyrate + NH3 - ? 18142 3.5.5.7 4-chlorobutyronitrile + H2O 135% activity compared to 3-cyanopyridine Cereibacter sphaeroides LHS-305 4-chlorobutyrate + NH3 - ? 18142 3.5.5.7 4-cyano-1-cyclohexene + H2O 7.03% of the activity with crotononitrile Rhodococcus rhodochrous 1-cyclohexene-4-carboxylate + NH3 - ? 18168 3.5.5.7 4-cyanopyridine + 2 H2O 50% of the activity with acrylonitrile Rhodococcus rhodochrous 4-pyridinecarboxylic acid + NH3 - ? 141126 3.5.5.7 4-cyanopyridine + 2 H2O 50% of the activity with acrylonitrile Rhodococcus rhodochrous tg1-A6 4-pyridinecarboxylic acid + NH3 - ? 141126 3.5.5.7 4-cyanopyridine + 2 H2O low activity Meyerozyma guilliermondii pyridine 4-carboxylic acid + NH3 - ? 371932 3.5.5.7 4-cyanopyridine + 2 H2O 225% activity compared to benzonitrile Fusarium proliferatum pyridine 4-carboxylic acid + NH3 - ? 371932 3.5.5.7 4-cyanopyridine + 2 H2O activity is 3fold higher compared to activity with 3-cyanopyridine Cupriavidus necator pyridine 4-carboxylic acid + NH3 - ? 371932 3.5.5.7 4-cyanopyridine + H2O 9.71% of the activity with crotononitrile Rhodococcus rhodochrous 4-carboxypyridine + NH3 - ? 18161 3.5.5.7 4-nitrobenzonitrile + H2O 151% activity compared to benzonitrile Fusarium proliferatum 4-nitrobenzoic acid + NH3 - ? 141132 3.5.5.7 acetamide + H2O - Meyerozyma guilliermondii ? - ? 140682 3.5.5.7 acetamide + H2O - Meyerozyma guilliermondii UFMG-Y65 ? - ? 140682 3.5.5.7 acetonitrile + 2 H2O 19.1% compared to the activity with 3-cyanopyridine Thermotoga maritima acetic acid + NH3 - ? 419314 3.5.5.7 acetonitrile + H2O - Rhodococcus rhodochrous acetate + NH3 - ? 18132 3.5.5.7 acetonitrile + H2O - Pseudomonas sp. acetate + NH3 - ? 18132 3.5.5.7 acetonitrile + H2O - Meyerozyma guilliermondii acetate + NH3 - ? 18132 3.5.5.7 acetonitrile + H2O 28.3% of the activity with crotononitrile Rhodococcus rhodochrous acetate + NH3 - ? 18132 3.5.5.7 acetonitrile + H2O 135% activity compared to benzonitrile Fusarium proliferatum acetate + NH3 - ? 18132 3.5.5.7 acetonitrile + H2O - Pseudomonas sp. S1 acetate + NH3 - ? 18132 3.5.5.7 acetonitrile + H2O 135% activity compared to benzonitrile Fusarium proliferatum AUF-2 acetate + NH3 - ? 18132 3.5.5.7 acetonitrile + H2O - Meyerozyma guilliermondii UFMG-Y65 acetate + NH3 - ? 18132 3.5.5.7 acetonitrile + H2O - Rhodococcus rhodochrous BX2 acetate + NH3 - ? 18132 3.5.5.7 acetonitrile + H2O - Streptomyces sp. acetic acid + NH3 - ? 141155 3.5.5.7 acetonitrile + H2O 5% activity compared to glutaronitrile uncultured bacterium ? - ? 368307 3.5.5.7 acrylamide + H2O - Meyerozyma guilliermondii ? - ? 95111 3.5.5.7 acrylamide + H2O - Meyerozyma guilliermondii UFMG-Y65 ? - ? 95111 3.5.5.7 acrylonitrile + 2 H2O - Achromobacter denitrificans acrylic acid + NH3 - ? 419327 3.5.5.7 acrylonitrile + 2 H2O 2.8% compared to the activity with 3-cyanopyridine Thermotoga maritima acrylic acid + NH3 - ? 419327 3.5.5.7 acrylonitrile + 2 H2O 5% compared to the activity with 3-hexenedinitrile Pseudomonas fluorescens acrylic acid + NH3 - ? 419327 3.5.5.7 acrylonitrile + 2 H2O acrylonitrile is the best substrates for nitC1-encoded nitrilase Achromobacter denitrificans acrylic acid + NH3 - ? 419327 3.5.5.7 acrylonitrile + 2 H2O activity is 2.2fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis acrylic acid + NH3 - ? 419327 3.5.5.7 acrylonitrile + 2 H2O acrylonitrile is the best substrates for nitC1-encoded nitrilase Achromobacter denitrificans C-32 acrylic acid + NH3 - ? 419327 3.5.5.7 acrylonitrile + 2 H2O - Achromobacter denitrificans C-32 acrylic acid + NH3 - ? 419327 3.5.5.7 acrylonitrile + 2 H2O activity is 2.2fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis Q4.6 acrylic acid + NH3 - ? 419327 3.5.5.7 acrylonitrile + H2O - Rhodococcus rhodochrous acrylic acid + NH3 - ? 18118 3.5.5.7 acrylonitrile + H2O - Streptomyces sp. acrylic acid + NH3 - ? 18118 3.5.5.7 acrylonitrile + H2O - Pseudomonas fluorescens acrylic acid + NH3 - ? 18118 3.5.5.7 acrylonitrile + H2O best substrate Rhodococcus rhodochrous acrylic acid + NH3 - ? 18118 3.5.5.7 acrylonitrile + H2O at 23% of the activity with adiponitrile Comamonas testosteroni acrylic acid + NH3 - ? 18118 3.5.5.7 acrylonitrile + H2O 348% of the activity with crotononitrile Rhodococcus rhodochrous acrylic acid + NH3 - ? 18118 3.5.5.7 acrylonitrile + H2O high activity Meyerozyma guilliermondii acrylic acid + NH3 - ? 18118 3.5.5.7 acrylonitrile + H2O 100% activity Pseudomonas sp. acrylic acid + NH3 - ? 18118 3.5.5.7 acrylonitrile + H2O 128% activity compared to benzonitrile Rhodococcus rhodochrous acrylic acid + NH3 - ? 18118 3.5.5.7 acrylonitrile + H2O 144% activity compared to racemic Ibu-CN Acinetobacter sp. acrylic acid + NH3 - ? 18118 3.5.5.7 acrylonitrile + H2O the enzyme shows higher activity with acrylonitrile as with benzonitrile Rhodococcus rhodochrous acrylic acid + NH3 - ? 18118 3.5.5.7 acrylonitrile + H2O 400% activity compared to benzonitrile Fusarium proliferatum acrylic acid + NH3 - ? 18118 3.5.5.7 acrylonitrile + H2O best substrate Rhodococcus rhodochrous tg1-A6 acrylic acid + NH3 - ? 18118 3.5.5.7 acrylonitrile + H2O - Pseudomonas fluorescens Pf-5 acrylic acid + NH3 - ? 18118 3.5.5.7 acrylonitrile + H2O 100% activity Pseudomonas sp. S1 acrylic acid + NH3 - ? 18118 3.5.5.7 acrylonitrile + H2O 144% activity compared to racemic Ibu-CN Acinetobacter sp. AK226 acrylic acid + NH3 - ? 18118 3.5.5.7 acrylonitrile + H2O 400% activity compared to benzonitrile Fusarium proliferatum AUF-2 acrylic acid + NH3 - ? 18118 3.5.5.7 acrylonitrile + H2O - Rhodococcus rhodochrous K22 acrylic acid + NH3 - ? 18118 3.5.5.7 acrylonitrile + H2O 348% of the activity with crotononitrile Rhodococcus rhodochrous K22 acrylic acid + NH3 - ? 18118 3.5.5.7 acrylonitrile + H2O the enzyme shows higher activity with acrylonitrile as with benzonitrile Rhodococcus rhodochrous K22 acrylic acid + NH3 - ? 18118 3.5.5.7 acrylonitrile + H2O - Streptomyces sp. MTCC 7546 acrylic acid + NH3 - ? 18118 3.5.5.7 acrylonitrile + H2O high activity Meyerozyma guilliermondii UFMG-Y65 acrylic acid + NH3 - ? 18118 3.5.5.7 acrylonitrile + H2O 128% activity compared to benzonitrile Rhodococcus rhodochrous J1 acrylic acid + NH3 - ? 18118 3.5.5.7 acrylonitrile + H2O - Rhodococcus rhodochrous BX2 acrylic acid + NH3 - ? 18118 3.5.5.7 acrylonitrile + H2O - Streptomyces sp. acrylate + NH3 - ? 372258 3.5.5.7 acrylonitrile + H2O - Rhodococcus rhodochrous acrylate + NH3 - ? 372258 3.5.5.7 acrylonitrile + H2O most effective substrate Pseudomonas sp. acrylate + NH3 - ? 372258 3.5.5.7 acrylonitrile + H2O 25.9% activity compared to 3-cyanopyridine Cereibacter sphaeroides acrylate + NH3 - ? 372258 3.5.5.7 adipamide + H2O - Meyerozyma guilliermondii ? - ? 372267 3.5.5.7 adiponitrile + 4 H2O 3% compared to the activity with 3-hexenedinitrile Pseudomonas fluorescens adipic acid + 2 NH3 - ? 455269 3.5.5.7 adiponitrile + 4 H2O 42% of the activity as compared to acrylonitrile Achromobacter denitrificans adipic acid + 2 NH3 - ? 455269 3.5.5.7 adiponitrile + 4 H2O activity is 1.14fold higher compared to activity with 3-cyanopyridine Cupriavidus necator adipic acid + 2 NH3 - ? 455269 3.5.5.7 adiponitrile + 4 H2O activity is 2.13fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis adipic acid + 2 NH3 - ? 455269 3.5.5.7 adiponitrile + 4 H2O activity is comparable to activity with acrylonitrile Achromobacter denitrificans adipic acid + 2 NH3 - ? 455269 3.5.5.7 adiponitrile + 4 H2O 42% of the activity as compared to acrylonitrile Achromobacter denitrificans C-32 adipic acid + 2 NH3 - ? 455269 3.5.5.7 adiponitrile + 4 H2O activity is comparable to activity with acrylonitrile Achromobacter denitrificans C-32 adipic acid + 2 NH3 - ? 455269 3.5.5.7 adiponitrile + H2O - Comamonas testosteroni adipic acid + NH3 - ? 18148 3.5.5.7 adiponitrile + H2O - Streptomyces sp. adipic acid + NH3 - ? 18148 3.5.5.7 adiponitrile + H2O - Rhodococcus rhodochrous adipic acid + NH3 - ? 18148 3.5.5.7 adiponitrile + H2O 110% of the activity with crotononitrile Rhodococcus rhodochrous adipic acid + NH3 - ? 18148 3.5.5.7 adiponitrile + H2O 100% activity Comamonas testosteroni adipic acid + NH3 - ? 18148 3.5.5.7 adiponitrile + H2O - Rhodococcus rhodochrous adipate + NH3 - ? 372268 3.5.5.7 adiponitrile + H2O - Pseudomonas sp. adipate + NH3 - ? 372268 3.5.5.7 adiponitrile + H2O - Meyerozyma guilliermondii adipate + NH3 - ? 372268 3.5.5.7 adiponitrile + H2O 91% activity compared to glutaronitrile uncultured bacterium adipate + NH3 - ? 372268 3.5.5.7 allylcyanide + 2 H2O 12% compared to the activity with 3-hexenedinitrile Pseudomonas fluorescens but-3-enoic acid + NH3 - ? 455284 3.5.5.7 aminoacetonitrile + H2O 30% of the activity with acrylonitrile Rhodococcus rhodochrous aminoacetic acid + NH3 - ? 404294 3.5.5.7 aminoacetonitrile + H2O 30% of the activity with acrylonitrile Rhodococcus rhodochrous tg1-A6 aminoacetic acid + NH3 - ? 404294 3.5.5.7 benzamide + H2O - Meyerozyma guilliermondii ? - ? 140684 3.5.5.7 benzonitrile + 2 H2O - Pseudomonas fluorescens benzoate + NH3 - ? 18154 3.5.5.7 benzonitrile + 2 H2O - Synechocystis sp. benzoate + NH3 - ? 18154 3.5.5.7 benzonitrile + 2 H2O at 4% of the activity with acrylonitrile Comamonas testosteroni benzoate + NH3 - ? 18154 3.5.5.7 benzonitrile + 2 H2O 27.1% of the activity with crotononitrile Rhodococcus rhodochrous benzoate + NH3 - ? 18154 3.5.5.7 benzonitrile + 2 H2O 100% activity Fusarium proliferatum benzoate + NH3 - ? 18154 3.5.5.7 benzonitrile + 2 H2O 55.4% activity compared to 3-cyanopyridine Cereibacter sphaeroides benzoate + NH3 - ? 18154 3.5.5.7 benzonitrile + 2 H2O 55.4% activity compared to 3-cyanopyridine Cereibacter sphaeroides LHS-305 benzoate + NH3 - ? 18154 3.5.5.7 benzonitrile + 2 H2O - Pseudomonas sp. benzoic acid + NH3 - ? 141120 3.5.5.7 benzonitrile + 2 H2O - Meyerozyma guilliermondii benzoic acid + NH3 - ? 141120 3.5.5.7 benzonitrile + 2 H2O - Rhodococcus rhodochrous benzoic acid + NH3 - ? 141120 3.5.5.7 benzonitrile + 2 H2O 100% activity Rhodococcus rhodochrous benzoic acid + NH3 - ? 141120 3.5.5.7 benzonitrile + 2 H2O 100% activity Synechocystis sp. benzoic acid + NH3 - ? 141120 3.5.5.7 benzonitrile + 2 H2O 3.8% activity compared to acrylonitrile Pseudomonas sp. benzoic acid + NH3 - ? 141120 3.5.5.7 benzonitrile + 2 H2O 4% activity compared to adiponitrile Comamonas testosteroni benzoic acid + NH3 - ? 141120 3.5.5.7 benzonitrile + 2 H2O 4.6% activity compared to fumaronitrile Acidovorax facilis benzoic acid + NH3 - ? 141120 3.5.5.7 benzonitrile + 2 H2O 94% activity compared to racemic Ibu-CN Acinetobacter sp. benzoic acid + NH3 - ? 141120 3.5.5.7 benzonitrile + 2 H2O 5% of the activity as compared to acrylonitrile Achromobacter denitrificans benzoic acid + NH3 - ? 141120 3.5.5.7 benzonitrile + 2 H2O 8% of the activity as compared to acrylonitrile Achromobacter denitrificans benzoic acid + NH3 - ? 141120 3.5.5.7 benzonitrile + 2 H2O activity is 2.24fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis benzoic acid + NH3 - ? 141120 3.5.5.7 benzonitrile + 2 H2O - Pseudomonas sp. S1 benzoic acid + NH3 - ? 141120 3.5.5.7 benzonitrile + 2 H2O 3.8% activity compared to acrylonitrile Pseudomonas sp. S1 benzoic acid + NH3 - ? 141120 3.5.5.7 benzonitrile + 2 H2O 94% activity compared to racemic Ibu-CN Acinetobacter sp. AK226 benzoic acid + NH3 - ? 141120 3.5.5.7 benzonitrile + 2 H2O - Rhodococcus rhodochrous K22 benzoic acid + NH3 - ? 141120 3.5.5.7 benzonitrile + 2 H2O 5% of the activity as compared to acrylonitrile Achromobacter denitrificans C-32 benzoic acid + NH3 - ? 141120 3.5.5.7 benzonitrile + 2 H2O 8% of the activity as compared to acrylonitrile Achromobacter denitrificans C-32 benzoic acid + NH3 - ? 141120 3.5.5.7 benzonitrile + 2 H2O activity is 2.24fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis Q4.6 benzoic acid + NH3 - ? 141120 3.5.5.7 benzonitrile + 2 H2O 4.6% activity compared to fumaronitrile Acidovorax facilis 72W benzoic acid + NH3 - ? 141120 3.5.5.7 benzonitrile + 2 H2O 100% activity Rhodococcus rhodochrous J1 benzoic acid + NH3 - ? 141120 3.5.5.7 beta-cyano-L-alanine + H2O - Arabidopsis thaliana asparagine + aspartic acid 60:40 mixture ? 372541 3.5.5.7 butyronitrile + 2 H2O 48.5% compared to the activity with 3-cyanopyridine Thermotoga maritima butyric acid + NH3 - ? 455419 3.5.5.7 butyronitrile + 2 H2O 75% of the activity as compared to acrylonitrile Achromobacter denitrificans butyric acid + NH3 - ? 455419 3.5.5.7 butyronitrile + 2 H2O 83% of the activity as compared to acrylonitrile Achromobacter denitrificans butyric acid + NH3 - ? 455419 3.5.5.7 butyronitrile + 2 H2O 75% of the activity as compared to acrylonitrile Achromobacter denitrificans C-32 butyric acid + NH3 - ? 455419 3.5.5.7 butyronitrile + 2 H2O 83% of the activity as compared to acrylonitrile Achromobacter denitrificans C-32 butyric acid + NH3 - ? 455419 3.5.5.7 butyronitrile + H2O - Pseudomonas sp. butyrate + NH3 - ? 18134 3.5.5.7 butyronitrile + H2O 18.0% of the activity with crotononitrile Rhodococcus rhodochrous butyrate + NH3 - ? 18134 3.5.5.7 butyronitrile + H2O 425% activity compared to benzonitrile Fusarium proliferatum butyrate + NH3 - ? 18134 3.5.5.7 butyronitrile + H2O - Pseudomonas sp. S1 butyrate + NH3 - ? 18134 3.5.5.7 butyronitrile + H2O - Meyerozyma guilliermondii butyric acid + NH3 - ? 372609 3.5.5.7 butyronitrile + H2O - Synechocystis sp. butyric acid + NH3 - ? 372609 3.5.5.7 butyronitrile + H2O 6% activity compared to glutaronitrile uncultured bacterium ? - ? 414403 3.5.5.7 capronitrile + H2O 38.7% of the activity with crotononitrile Rhodococcus rhodochrous hexanoate + NH3 - ? 18137 3.5.5.7 chloroacetonitrile + H2O 61.7% of the activity with crotononitrile Rhodococcus rhodochrous chloroacetate + NH3 - ? 18140 3.5.5.7 cinnamonitrile + 2 H2O activity is 8fold higher compared to activity with 3-cyanopyridine Cupriavidus necator cinnamic acid + NH3 - ? 426110 3.5.5.7 cinnamonitrile + H2O 31.2% of the activity with crotononitrile Rhodococcus rhodochrous cinnamate + NH3 - ? 18171 3.5.5.7 cinnamonitrile + H2O 28% activity compared to glutaronitrile uncultured bacterium cinnamate + NH3 - ? 18171 3.5.5.7 cis-crotononitrile + H2O - Rhodococcus rhodochrous crotonic acid + NH3 - ? 433573 3.5.5.7 cis-crotononitrile + H2O - Rhodococcus rhodochrous BX2 crotonic acid + NH3 - ? 433573 3.5.5.7 crotonitrile + H2O - Rhodococcus rhodochrous crotonic acid + NH3 - ? 18119 3.5.5.7 crotonitrile + H2O - Streptomyces sp. crotonic acid + NH3 - ? 18119 3.5.5.7 crotonitrile + H2O - Rhodococcus rhodochrous K22 crotonic acid + NH3 - ? 18119 3.5.5.7 crotonitrile + H2O - Streptomyces sp. MTCC 7546 crotonic acid + NH3 - ? 18119 3.5.5.7 crotonitrile + H2O 7% activity compared to glutaronitrile uncultured bacterium ? - ? 414457 3.5.5.7 cyano-5-valeramide + H2O at 28% of the activity with adiponitrile Comamonas testosteroni glutaric acid 5-amide + NH3 - ? 18176 3.5.5.7 cyano-5-valeric acid + H2O + H2O at 28% of the activity with adiponitrile Comamonas testosteroni ? - ? 18177 3.5.5.7 cyanoacetic acid ethyl ester + H2O 117% of the activity withcrotononitrile Rhodococcus rhodochrous acetic acid ethyl ester + NH3 - ? 18143 3.5.5.7 cyanopyrazine + H2O 17.3% of the activity with crotononitrile Rhodococcus rhodochrous pyrazine-2-carboxylate + NH3 - ? 18162 3.5.5.7 cyclopentanecarbonitrile + H2O 11.7% of the activity with crotononitrile Rhodococcus rhodochrous cyclopentanoic acid + NH3 - ? 18167 3.5.5.7 cyclopentanocarbonitrile + H2O - Meyerozyma guilliermondii cyclopentanocarbonate + NH3 - ? 372799 3.5.5.7 cyclopropanecarbonitrile + H2O 22.9% of the activity with crotononitrile Rhodococcus rhodochrous cyclopropanoic acid + NH3 - ? 18166 3.5.5.7 diaminomaleonitrile + H2O 18.6% of the activity with crotononitrile Rhodococcus rhodochrous diaminomaleic acid + NH3 - ? 18125 3.5.5.7 dimethylaminopropionitrile + 2 H2O 50.5% compared to the activity with 3-cyanopyridine Thermotoga maritima dimethylaminopropionic acid + NH3 - ? 455584 3.5.5.7 dimethylmalononitrile + H2O - Synechocystis sp. dimethylmalonic acid + NH3 - ? 397073 3.5.5.7 diphenylacetonitrile + H2O 72% activity compared to benzonitrile Fusarium proliferatum diphenylacetic acid + NH3 - ? 433644 3.5.5.7 dodecanenitrile + 2 H2O activity is 2.46fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis dodecanoic acid + NH3 - ? 455599 3.5.5.7 dodecanenitrile + H2O 13.5% activity compared to 3-cyanopyridine Cereibacter sphaeroides dodecanoate + NH3 - ? 433656 3.5.5.7 fumaronitrile + 4 H2O activity is 2.21fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis fumaric acid + 2 NH3 - ? 455677 3.5.5.7 fumaronitrile + 4 H2O activity is 36fold higher compared to activity with 3-cyanopyridine Cupriavidus necator fumaric acid + 2 NH3 - ? 455677 3.5.5.7 fumaronitrile + H2O - Streptomyces sp. fumaric acid + NH3 - ? 18124 3.5.5.7 fumaronitrile + H2O - Synechocystis sp. fumaric acid + NH3 - ? 18124 3.5.5.7 fumaronitrile + H2O - Pyrococcus abyssi fumaric acid + NH3 - ? 18124 3.5.5.7 fumaronitrile + H2O - Pseudomonas fluorescens fumaric acid + NH3 - ? 18124 3.5.5.7 fumaronitrile + H2O preferred substrate Pyrococcus abyssi fumaric acid + NH3 - ? 18124 3.5.5.7 fumaronitrile + H2O 27.4% of the activity with crotononitrile Rhodococcus rhodochrous fumaric acid + NH3 - ? 18124 3.5.5.7 fumaronitrile + H2O 100% activity Acidovorax facilis fumaric acid + NH3 - ? 18124 3.5.5.7 fumaronitrile + H2O 12000% activity compared to benzonitrile Synechocystis sp. fumaric acid + NH3 - ? 18124 3.5.5.7 fumaronitrile + H2O - Pseudomonas fluorescens Pf-5 fumaric acid + NH3 - ? 18124 3.5.5.7 fumaronitrile + H2O - Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 fumaric acid + NH3 - ? 18124 3.5.5.7 fumaronitrile + H2O preferred substrate Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 fumaric acid + NH3 - ? 18124 3.5.5.7 fumaronitrile + H2O - Streptomyces sp. MTCC 7546 fumaric acid + NH3 - ? 18124 3.5.5.7 fumaronitrile + H2O 100% activity Acidovorax facilis 72W fumaric acid + NH3 - ? 18124 3.5.5.7 fumaronitrile + H2O best substrate Synechocystis sp. fumarate + NH3 - ? 433749 3.5.5.7 fumaronitrile + H2O 209.8 activity compared to 3-cyanopyridine Cereibacter sphaeroides fumarate + NH3 - ? 433749 3.5.5.7 glutaramide + H2O - Meyerozyma guilliermondii ? - ? 373304 3.5.5.7 glutaronitrile + H2O - Rhodococcus rhodochrous glutarate + NH3 - ? 18147 3.5.5.7 glutaronitrile + H2O - Pseudomonas sp. glutarate + NH3 - ? 18147 3.5.5.7 glutaronitrile + H2O - Meyerozyma guilliermondii glutarate + NH3 - ? 18147 3.5.5.7 glutaronitrile + H2O - Pseudomonas fluorescens glutarate + NH3 - ? 18147 3.5.5.7 glutaronitrile + H2O 345% of the activity with crotononitrile Rhodococcus rhodochrous glutarate + NH3 - ? 18147 3.5.5.7 glutaronitrile + H2O 100% activity uncultured bacterium glutarate + NH3 - ? 18147 3.5.5.7 glutaronitrile + H2O - Pseudomonas sp. S1 glutarate + NH3 - ? 18147 3.5.5.7 glutaronitrile + H2O - Rhodococcus rhodochrous K22 glutarate + NH3 - ? 18147 3.5.5.7 glutaronitrile + H2O - Meyerozyma guilliermondii UFMG-Y65 glutarate + NH3 - ? 18147 3.5.5.7 glutaronitrile + H2O - Streptomyces sp. glutaric acid + NH3 - ? 397275 3.5.5.7 glutaronitrile + H2O - Rhodococcus rhodochrous glutaric acid + NH3 - ? 397275 3.5.5.7 glutaronitrile + H2O 319% activity compared to benzonitrile Fusarium proliferatum glutaric acid + NH3 - ? 397275 3.5.5.7 glycolonitrile + 2 H2O preferred substrate Alcaligenes sp. glycolic acid + NH3 - ? 420746 3.5.5.7 glycolonitrile + 2 H2O preferred substrate Rhodococcus erythropolis glycolic acid + NH3 - ? 420746 3.5.5.7 glycolonitrile + 2 H2O activity is 15.9fold higher compared to activity with 3-cyanopyridine Cupriavidus necator glycolic acid + NH3 - ? 420746 3.5.5.7 glycolonitrile + 2 H2O preferred substrate Rhodococcus erythropolis CGMCC 1.2362 glycolic acid + NH3 - ? 420746 3.5.5.7 glycolonitrile + 2 H2O preferred substrate Alcaligenes sp. ECU0401 glycolic acid + NH3 - ? 420746 3.5.5.7 glycolonitrile + H2O 40% of the activity with acrylonitrile Rhodococcus rhodochrous ammonium glycolate + ? - ? 397358 3.5.5.7 glycolonitrile + H2O 40% of the activity with acrylonitrile Rhodococcus rhodochrous tg1-A6 ammonium glycolate + ? - ? 397358 3.5.5.7 heptanenitrile + H2O - Synechocystis sp. heptanoic acid + NH3 - ? 397412 3.5.5.7 hexanedinitrile + 2 H2O 111% compared to the activity with 3-cyanopyridine Thermotoga maritima ? - ? 455767 3.5.5.7 hexanenitrile + H2O - Synechocystis sp. hexanoic acid + NH3 - ? 397422 3.5.5.7 hydrocinnamonitrile + H2O - Streptomyces sp. hydrocinnamic acid + NH3 - ? 397444 3.5.5.7 iminodiacetonitrile + 2 H2O 261% of the activity with crotononitrile Rhodococcus rhodochrous iminodiacetic acid + 2 NH3 - ? 18153 3.5.5.7 iminodiacetonitrile + 2 H2O 25% of the activity with acrylonitrile Rhodococcus rhodochrous iminodiacetic acid + 2 NH3 - ? 18153 3.5.5.7 iminodiacetonitrile + 2 H2O 25% of the activity with acrylonitrile Rhodococcus rhodochrous tg1-A6 iminodiacetic acid + 2 NH3 - ? 18153 3.5.5.7 iminodiacetonitrile + 2 H2O activity is 2.34fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis iminodiacetic acid + NH3 - ? 455780 3.5.5.7 iminodiacetonitrile + 4 H2O activity is 39% compared to activity with 3-cyanopyridine Cupriavidus necator iminodiacetic acid + 2 NH3 - ? 455781 3.5.5.7 indole-3-acetonitrile + 2 H2O activity is 48% compared to activity with 3-cyanopyridine Cupriavidus necator indol-3-acetic acid + NH3 - ? 455788 3.5.5.7 isobutyramide + H2O - Meyerozyma guilliermondii ? - ? 373481 3.5.5.7 isobutyronitrile + H2O - Streptomyces sp. isobutyrate + NH3 - ? 18135 3.5.5.7 isobutyronitrile + H2O 6.74% of the activity with crotononitrile Rhodococcus rhodochrous isobutyrate + NH3 - ? 18135 3.5.5.7 isobutyronitrile + H2O - Meyerozyma guilliermondii isobutyric acid + NH3 - ? 373483 3.5.5.7 isocapronitrile + H2O 41.5% of the activity with crotononitrile Rhodococcus rhodochrous isohexanoate + NH3 - ? 18138 3.5.5.7 isophthalonitrile + H2O 66.1% of the activity with crotononitrile Rhodococcus rhodochrous isophthalate + NH3 - ? 18157 3.5.5.7 isovaleronitrile + H2O - Streptomyces sp. isovaleric acid + NH3 - ? 384948 3.5.5.7 m-tolunitrile + H2O - Rhodococcus rhodochrous m-methylbenzoate + NH3 - ? 397713 3.5.5.7 malonitrile + H2O - Pyrococcus abyssi malonic acid + NH3 - ? 414826 3.5.5.7 malononitrile + 2 H2O 120.5% compared to the activity with 3-cyanopyridine Thermotoga maritima malonic acid + NH3 - ? 455950 3.5.5.7 malononitrile + H2O - Pyrococcus abyssi malonic acid + NH3 - ? 18145 3.5.5.7 malononitrile + H2O preferred substrate Pyrococcus abyssi malonic acid + NH3 - ? 18145 3.5.5.7 malononitrile + H2O 45.1% of the activity with crotononitrile Rhodococcus rhodochrous malonic acid + NH3 - ? 18145 3.5.5.7 malononitrile + H2O - Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 malonic acid + NH3 - ? 18145 3.5.5.7 malononitrile + H2O preferred substrate Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 malonic acid + NH3 - ? 18145 3.5.5.7 mandelonitrile + 2 H2O - Streptomyces sp. mandelic acid + NH3 - ? 18107 3.5.5.7 mandelonitrile + 2 H2O 69% activity compared to benzonitrile Fusarium proliferatum mandelic acid + NH3 - ? 18107 3.5.5.7 mandelonitrile + 2 H2O 69% activity compared to benzonitrile Fusarium proliferatum AUF-2 mandelic acid + NH3 - ? 18107 3.5.5.7 mandelonitrile + 2 H2O - Streptomyces sp. MTCC 7546 mandelic acid + NH3 - ? 18107 3.5.5.7 metacrylonitrile + H2O - Meyerozyma guilliermondii metacrylic acid + NH3 - ? 373812 3.5.5.7 methacrylonitrile + H2O 143% of the activity with crotononitrile Rhodococcus rhodochrous methacrylic acid + NH3 - ? 18120 3.5.5.7 methacrylonitrile + H2O 143% of the activity with crotononitrile Rhodococcus rhodochrous K22 methacrylic acid + NH3 - ? 18120 3.5.5.7 methoxyacetonitrile + H2O 37.4% of the activity with crotononitrile Rhodococcus rhodochrous methoxyacetate + NH3 - ? 18139 3.5.5.7 additional information lack of acide amide production Rhodococcus rhodochrous ? - ? 89 3.5.5.7 additional information the enzyme catalyzes regioselectively the conversion of aliphatic dinitriles to cyanocarboxylic acids Acidovorax facilis ? - ? 89 3.5.5.7 additional information compounds with a nitrile group bound to an aromatic ring or amino acid are not hydrolyzed by recombinant nitrilase Pyrococcus abyssi ? - ? 89 3.5.5.7 additional information isovaleronitrile, benzonitrile, and pyridine-3-carbonitrile are weak substrates Streptomyces sp. ? - ? 89 3.5.5.7 additional information enzyme displays a high specificity towards dinitrile substrates Pseudomonas fluorescens ? - ? 89 3.5.5.7 additional information Nit1 does not hydrolyze malononitrile, mandelonitrile, 4-cyanobenzoic acid, 3-ethoxybenzonitrile, 4-hydroxybenzonitrile, alpha-methylbenzyl cyanide, acrylonitrile, 4-tolunitrile, and benzonitrile uncultured bacterium ? - ? 89 3.5.5.7 additional information Nit1 is an aliphatic nitrilase favoring dinitriles over mononitriles uncultured bacterium ? - ? 89 3.5.5.7 additional information the cells are also active on benzoic acid to a lesser extent compared to aliphatic nitriles Alcaligenes sp. ? - ? 89 3.5.5.7 additional information the cells are also active on benzoic acid to a lesser extent compared to aliphatic nitriles Rhodococcus erythropolis ? - ? 89 3.5.5.7 additional information no activity with fumaronitrile and succinonitrile Fusarium proliferatum ? - ? 89 3.5.5.7 additional information no activity with iminodiacetonitrile, glycolonitrile, 3-hydroxyglutaronitrile, phenylacetonitrile, 1,2-phenylenediacetonitrile, mandelonitrile, and indole-3-acetonitrile Cereibacter sphaeroides ? - ? 89 3.5.5.7 additional information no activity with mandelonitrile, glycolonitrile Thermotoga maritima ? - - 89 3.5.5.7 additional information the enzyme shows a broad hydrolytic activity toward aliphatic and heterocyclic nitriles and shows high tolerance of 3-cyanopyridine. No activity with: benzonitrile, phenylacetonitrile, 4-chlorobenzyl cyanide, mandelonitrile, 2-chloromandelonitrile, alpha-methylphenylacetonitrile, 1,2-phenylenediacetonitrile, dodecanenitrile Cupriavidus necator ? - - 89 3.5.5.7 additional information the enzyme shows broad substrate specificity towards aliphatic, aromatic, and heterocyclic nitriles Pannonibacter carbonis ? - - 89 3.5.5.7 additional information the enzyme shows preference for unsaturated aliphatic substrates containing 5-6 carbon atoms. Increased reaction rates are also found for aliphatic nitriles carrying electron withdrawing substituents (e.g. chloro- or hydroxy-groups) close to the nitrile group. Aliphatic dinitriles are attacked only at one of the nitrile groups and with most of the tested dinitriles the monocarboxylates are detected as major products. Substrates converted with 1% less compared to the activity with 3-hexenedinitrile: 2-methylbutyronitrile, decanedinitrile, octanedinitrile, cis,trans-crotononitrile, popionitrile, isovaleronitrile, lactonitrile, butyronitrile Pseudomonas fluorescens ? - - 89 3.5.5.7 additional information enzyme displays a high specificity towards dinitrile substrates Pseudomonas fluorescens Pf-5 ? - ? 89 3.5.5.7 additional information no activity with iminodiacetonitrile, glycolonitrile, 3-hydroxyglutaronitrile, phenylacetonitrile, 1,2-phenylenediacetonitrile, mandelonitrile, and indole-3-acetonitrile Cereibacter sphaeroides LHS-305 ? - ? 89 3.5.5.7 additional information no activity with fumaronitrile and succinonitrile Fusarium proliferatum AUF-2 ? - ? 89 3.5.5.7 additional information the cells are also active on benzoic acid to a lesser extent compared to aliphatic nitriles Rhodococcus erythropolis CGMCC 1.2362 ? - ? 89 3.5.5.7 additional information compounds with a nitrile group bound to an aromatic ring or amino acid are not hydrolyzed by recombinant nitrilase Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 ? - ? 89 3.5.5.7 additional information isovaleronitrile, benzonitrile, and pyridine-3-carbonitrile are weak substrates Streptomyces sp. MTCC 7546 ? - ? 89 3.5.5.7 additional information the cells are also active on benzoic acid to a lesser extent compared to aliphatic nitriles Alcaligenes sp. ECU0401 ? - ? 89 3.5.5.7 additional information the enzyme shows broad substrate specificity towards aliphatic, aromatic, and heterocyclic nitriles Pannonibacter carbonis Q4.6 ? - - 89 3.5.5.7 additional information the enzyme catalyzes regioselectively the conversion of aliphatic dinitriles to cyanocarboxylic acids Acidovorax facilis 72W ? - ? 89 3.5.5.7 N-butyronitrile + 2 H2O - Alcaligenes sp. N-butyric acid + NH3 - ? 421046 3.5.5.7 N-butyronitrile + 2 H2O - Rhodococcus erythropolis N-butyric acid + NH3 - ? 421046 3.5.5.7 N-butyronitrile + 2 H2O - Rhodococcus erythropolis CGMCC 1.2362 N-butyric acid + NH3 - ? 421046 3.5.5.7 N-butyronitrile + 2 H2O - Alcaligenes sp. ECU0401 N-butyric acid + NH3 - ? 421046 3.5.5.7 N-methyl-beta-alaninenitrile + H2O 11.4% of the activity with crotononitrile Rhodococcus rhodochrous N-methyl-beta-Ala + NH3 - ? 18144 3.5.5.7 pentanenitrile + 2 H2O 56.3% compared to the activity with 3-cyanopyridine Thermotoga maritima pentanoic acid + NH3 - ? 456249 3.5.5.7 pentanenitrile + 2 H2O activity is 2.39fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis pentanoic acid + NH3 - ? 456249 3.5.5.7 pentanenitrile + H2O 62% activity compared to 3-cyanopyridine Cereibacter sphaeroides pentanoate + NH3 - ? 434120 3.5.5.7 phenylacetonitrile + 2 H2O 27.3% of the activity with crotononitrile Rhodococcus rhodochrous phenylacetate + NH3 - ? 18172 3.5.5.7 phenylacetonitrile + 2 H2O - Pseudomonas sp. phenylacetic acid + NH3 - ? 363353 3.5.5.7 phenylacetonitrile + 2 H2O 16.3% compared to the activity with 3-cyanopyridine Thermotoga maritima phenylacetic acid + NH3 - ? 363353 3.5.5.7 phenylacetonitrile + 2 H2O 5% of the activity as compared to acrylonitrile Achromobacter denitrificans phenylacetic acid + NH3 - ? 363353 3.5.5.7 phenylacetonitrile + 2 H2O best substrate for nitC2-encoded nitrilase. Activity is 16.7fold higher as compared to activity with acrylonitrile Achromobacter denitrificans phenylacetic acid + NH3 - ? 363353 3.5.5.7 phenylacetonitrile + 2 H2O - Pseudomonas sp. S1 phenylacetic acid + NH3 - ? 363353 3.5.5.7 phenylacetonitrile + 2 H2O 5% of the activity as compared to acrylonitrile Achromobacter denitrificans C-32 phenylacetic acid + NH3 - ? 363353 3.5.5.7 phenylacetonitrile + 2 H2O best substrate for nitC2-encoded nitrilase. Activity is 16.7fold higher as compared to activity with acrylonitrile Achromobacter denitrificans C-32 phenylacetic acid + NH3 - ? 363353 3.5.5.7 pimelonitrile + 4 H2O activity is 85% compared to activity with 3-cyanopyridine Cupriavidus necator pimelic acid + 2 NH3 - ? 456273 3.5.5.7 pimelonitrile + H2O 27.3% of the activity with crotononitrile Rhodococcus rhodochrous pimelic acid + NH3 - ? 18149 3.5.5.7 piperonylonitrile + H2O 6.8% of the activity with crotononitrile Rhodococcus rhodochrous (3,4-methylenedioxy)benzoate + NH3 - ? 18165 3.5.5.7 potassium cyanide + H2O - Pseudomonas sp. ? - ? 374399 3.5.5.7 propionitrile + 2 H2O 33.2% compared to the activity with 3-cyanopyridine Thermotoga maritima propionic acid + NH3 - ? 456295 3.5.5.7 propionitrile + H2O - Pseudomonas fluorescens propionate + NH3 - ? 18133 3.5.5.7 propionitrile + H2O at 6% of the activity with acrylonitrile Comamonas testosteroni propionate + NH3 - ? 18133 3.5.5.7 propionitrile + H2O 12.7% of the activity with crotononitrile Rhodococcus rhodochrous propionate + NH3 - ? 18133 3.5.5.7 propionitrile + H2O - Pseudomonas fluorescens Pf-5 propionate + NH3 - ? 18133 3.5.5.7 propionitrile + H2O - Meyerozyma guilliermondii propionic acid + NH3 - ? 141136 3.5.5.7 propionitrile + H2O - Streptomyces sp. propionic acid + NH3 - ? 141136 3.5.5.7 propionitrile + H2O 416% activity Fusarium proliferatum propionic acid + NH3 - ? 141136 3.5.5.7 propionitrile + H2O 5% activity compared to glutaronitrile uncultured bacterium ? - ? 368308 3.5.5.7 R-CN + H2O enzyme uses both aliphatic and aromatic nitriles, no formation of amide intermediate Pseudomonas sp. R-COOH + NH3 - ? 374526 3.5.5.7 racemic Ibu-CN + 2 H2O 100% activity Acinetobacter sp. ibuprofen + NH3 - ? 415179 3.5.5.7 racemic Ibu-CN + 2 H2O 100% activity Acinetobacter sp. AK226 ibuprofen + NH3 - ? 415179 3.5.5.7 sebaconitrile + 4 H2O activity is 1.66fold higher compared to activity with 3-cyanopyridine Cupriavidus necator sebaconic acid + 2 NH3 - ? 456657 3.5.5.7 sebaconitrile + 4 H2O activity is 1.92fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis sebaconic acid + 2 NH3 - ? 456657 3.5.5.7 sebaconitrile + H2O - Rhodococcus rhodochrous ? + NH3 - ? 18151 3.5.5.7 sebaconitrile + H2O - Pseudomonas fluorescens ? + NH3 - ? 18151 3.5.5.7 sebaconitrile + H2O 15.7% of the activity with crotononitrile Rhodococcus rhodochrous ? + NH3 - ? 18151 3.5.5.7 sebaconitrile + H2O - Streptomyces sp. sebaconic acid + NH3 - ? 398307 3.5.5.7 suberonitrile + H2O 21.4% of the activity with crotononitrile Rhodococcus rhodochrous decanedioic acid + NH3 - ? 18150 3.5.5.7 succinamide + H2O - Meyerozyma guilliermondii ? - ? 374659 3.5.5.7 succinodinitrile + H2O - Pseudomonas sp. ? - ? 374664 3.5.5.7 succinonitrile + 2 H2O 137.7% compared to the activity with 3-cyanopyridine Thermotoga maritima succinic acid + NH3 - ? 419606 3.5.5.7 succinonitrile + 4 H2O activity is 1.97fold higher compared to activity with 3-cyanopyridine Cupriavidus necator succinic acid + 2 NH3 - ? 456712 3.5.5.7 succinonitrile + 4 H2O activity is 2.64fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis succinic acid + 2 NH3 - ? 456712 3.5.5.7 succinonitrile + H2O - Rhodococcus rhodochrous succinate + NH3 - ? 18146 3.5.5.7 succinonitrile + H2O - Pseudomonas fluorescens succinate + NH3 - ? 18146 3.5.5.7 succinonitrile + H2O 271% of the activity with crotononitrile Rhodococcus rhodochrous succinate + NH3 - ? 18146 3.5.5.7 succinonitrile + H2O 76% activity compared to glutaronitrile uncultured bacterium succinate + NH3 - ? 18146 3.5.5.7 succinonitrile + H2O 66.3% activity compared to 3-cyanopyridine Cereibacter sphaeroides succinate + NH3 - ? 18146 3.5.5.7 succinonitrile + H2O - Pseudomonas fluorescens Pf-5 succinate + NH3 - ? 18146 3.5.5.7 succinonitrile + H2O 66.3% activity compared to 3-cyanopyridine Cereibacter sphaeroides LHS-305 succinate + NH3 - ? 18146 3.5.5.7 succinonitrile + H2O - Streptomyces sp. succinic acid + NH3 - ? 374665 3.5.5.7 succinonitrile + H2O best substrate Meyerozyma guilliermondii succinic acid + NH3 - ? 374665 3.5.5.7 thiophene-3-carbonitrile + 2 H2O activity is 2.42fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis thiophene-3-carboxylic acid + NH3 - ? 456832 3.5.5.7 thiophene-3-carbonitrile + H2O 51.2% activity compared to 3-cyanopyridine Cereibacter sphaeroides ? - ? 434435 3.5.5.7 trans-crotononitrile + H2O - Rhodococcus rhodochrous crotonic acid + NH3 - ? 434438 3.5.5.7 trans-crotononitrile + H2O - Rhodococcus rhodochrous BX2 crotonic acid + NH3 - ? 434438 3.5.5.7 valeronitrile + 2 H2O activity is 99% compared to activity with 3-cyanopyridine Cupriavidus necator valeric acid + NH3 - ? 419624 3.5.5.7 valeronitrile + H2O 40.8% of the activity with crotononitrile Rhodococcus rhodochrous pentanoate + NH3 - ? 18136 3.5.5.7 valeronitrile + H2O - Synechocystis sp. valeric acid + NH3 - ? 385908 3.5.5.7 valeronitrile + H2O 25% compared to the activity with 3-hexenedinitrile Pseudomonas fluorescens valeric acid + NH3 - ? 385908