3.5.5.4	3-cyano-L-alanine + 2 H2O	-	Pseudomonas oleovorans	L-aspartate + NH3	-	?	454999	
3.5.5.4	3-cyano-L-alanine + 2 H2O	-	Pseudomonas oleovorans CECT 5344	L-aspartate + NH3	-	?	454999	
3.5.5.4	3-cyano-L-alanine + H2O	-	Zea mays	L-aspartate + NH3	-	?	363207	
3.5.5.4	3-cyano-L-alanine + H2O	-	Arabidopsis thaliana	L-aspartate + NH3	-	?	363207	
3.5.5.4	3-cyano-L-alanine + H2O	-	Pseudomonas fluorescens	L-aspartate + NH3	-	?	363207	
3.5.5.4	3-cyano-L-alanine + H2O	-	Brassica rapa	L-aspartate + NH3	-	?	363207	
3.5.5.4	3-cyano-L-alanine + H2O	high activity substrate for isozyme complexes NIT4A/B2 and NIT4A/B1	Sorghum bicolor	L-aspartate + NH3	-	ir	363207	
3.5.5.4	3-cyano-L-alanine + H2O	-	Pseudomonas fluorescens SBW25	L-aspartate + NH3	-	?	363207	
3.5.5.4	beta-cyano-L-alanine + H2O	-	Escherichia coli	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	-	Priestia megaterium	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	-	Chromobacterium violaceum	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	L-asparagine is formed as an intermediate	Triticum aestivum	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	L-asparagine is formed as an intermediate	Hordeum vulgare	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	L-asparagine is formed as an intermediate	Zea mays	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	L-asparagine is formed as an intermediate	Pinus taeda	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	L-asparagine is formed as an intermediate	Nicotiana tabacum	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	L-asparagine is formed as an intermediate	Glycine max	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	L-asparagine is formed as an intermediate	Arabidopsis thaliana	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	L-asparagine is formed as an intermediate	Ricinus communis	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	L-asparagine is formed as an intermediate	Solanum lycopersicum	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	L-asparagine is formed as an intermediate	Lactuca sativa	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	L-asparagine is formed as an intermediate	Allium cepa	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	L-asparagine is formed as an intermediate	Oryza sativa	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	L-asparagine is formed as an intermediate	Mesembryanthemum crystallinum	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	L-asparagine is formed as an intermediate	Linum usitatissimum	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	L-asparagine is formed as an intermediate	Manihot esculenta	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	L-asparagine is formed as an intermediate	Physcomitrium patens	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	L-asparagine is formed as an intermediate	Brassica rapa	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	L-asparagine is formed as an intermediate	Lupinus angustifolius	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	L-asparagine is formed as an intermediate	Carica papaya	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	L-asparagine is formed as an intermediate	Tropaeolum majus	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	L-asparagine is formed as an intermediate	Vitis vinifera	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	L-asparagine is formed as an intermediate	Saccharum officinarum	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	L-asparagine is formed as an intermediate	Populus trichocarpa	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	L-asparagine is formed as an intermediate	Medicago truncatula	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	L-asparagine is formed as an intermediate	Curcuma longa	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	L-asparagine is formed as an intermediate	Selaginella moellendorffii	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	L-asparagine is formed as an intermediate	Capsella rubella	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	L-asparagine is formed as an intermediate	Tarenaya spinosa	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	the reaction is catalyzed only by isoform NIT4A, L-asparagine is formed as an intermediate	Lotus japonicus	L-aspartate + NH3	-	?	414370	
3.5.5.4	beta-cyano-L-alanine + H2O	the reaction is catalyzed only by isoform NIT4A, L-asparagine is formed as an intermediate	Sorghum bicolor	L-aspartate + NH3	-	?	414370	
3.5.5.4	additional information	no substrates are various nitrile or amide compounds, overview	Pseudomonas sp.	?	-	?	89	
3.5.5.4	additional information	no substrate: indole-3-acetonitrile	Lupinus angustifolius	?	-	?	89	
3.5.5.4	additional information	does not use indole-3-acetonitrile as a substrate	Zea mays	?	-	?	89	
3.5.5.4	additional information	indole-3-acetonitrile is no substrate for NIT-T4	Brassica rapa	?	-	?	89	
3.5.5.4	additional information	recombinant NIT4 isozymes NIT4A, NIT4B1, and NIT4B2 do not hydrolyze 3-cyano-L-alanine, benzonitrile, 4-hydroxybenzonitrile, butyronitrile, and 4-pentenenitrile	Sorghum bicolor	?	-	?	89	
3.5.5.4	additional information	isoform NIT4B does not show beta-cyano-L-alanine-hydrolyzing activity	Lotus japonicus	?	-	?	89	
3.5.5.4	additional information	isoform NIT4B1 does not show any beta-cyano-L-alanine-hydrolyzing activity	Sorghum bicolor	?	-	?	89	
3.5.5.4	additional information	no substrates are various nitrile or amide compounds, overview	Pseudomonas sp. 13	?	-	?	89	
3.5.5.4	phenyl-3-propionitrile + H2O	-	Lupinus angustifolius	phenyl-3-propionic acid + NH3	-	?	385537	
3.5.5.4	3-cyano-L-alanine + H2O	-	Lupinus angustifolius	L-aspartate + NH3	enzyme has additional hydratase activity for 3-cyano-L-alanine, ratio of hydratase activity to nitrilase activity is 3.3:1. L-asparagine and L-aspartate are formed at the same time, asparagine is not an intermediate	?	363207	
3.5.5.4	3-cyano-L-alanine + H2O	-	Lupinus angustifolius	L-aspartate + NH3	enzyme has additional hydratase activity for 3-cyano-L-alanine, ratio of hydratase activity to nitrilase activity is 4:1. L-asparagine and L-aspartate are formed at the same time, asparagine is not an intermediate	?	363207	
3.5.5.4	3-cyano-L-alanine + H2O	-	Pseudomonas sp.	L-aspartate + NH3	L-asparagine is formed as an intermediate	?	363207	
3.5.5.4	3-cyano-L-alanine + H2O	-	Pseudomonas sp. 13	L-aspartate + NH3	L-asparagine is formed as an intermediate	?	363207