3.5.1.87 an N-carbamoyl-L-2-amino acid + H2O - Brevibacillus reuszeri an L-2-amino acid + NH3 + CO2 - ? 432666 3.5.1.87 additional information strictly specific for the L-form of N-carbamoyl-amino acids as substrates and exhibits high activity in the hydrolysis of N-carbamoyl-L-cysteine as substrate Pseudomonas sp. ON4a ? - ? 89 3.5.1.87 additional information the enzyme in this study is strigently L-specific Geobacillus kaustophilus ? - ? 89 3.5.1.87 additional information catalytic promiscuity of L-N-carbamoylase from Geobacillus stearothermophilus CECT43, substrate specificity with different N-formyl- and N-carbamoyl-DL-amino acids, overview. No N-formyl-DL-tert-leucine Geobacillus stearothermophilus ? - ? 89 3.5.1.87 additional information the enzyme is a strict enantiospecific L-N-carbamoylase. Development of a bienzymatic system comprising an N-succinylamino acid racemase from Geobacillus kaustophilus CECT4264 and the enantiospecific L-N-carbamoylase from Geobacillus stearothermophilus CECT43. The biocatalyst system is able to produce optically pure natural and non-natural L-amino acids starting from racemic mixtures of N-acetyl-, N-formyl- and N-carbamoyl-amino acids by dynamic kinetic resolution. The fastest conversion rate is found with N-formyl-aminoacids, followed by N-carbamoyl- and N-acetyl-amino acids, and the an N-succinylamino acid racemase proves to be the limiting stepof the system due to its lower specific activity, overview Geobacillus stearothermophilus ? - ? 89 3.5.1.87 additional information the enzyme shows stereospecificity and a broad substrate specificity. No or poor activitz with N-carbamoyl-alpha-alanine, N-carbamoyl-glycine, N-carbamoyl-beta-alanine, and N-carbamoyl-alpha-aminoisobutyrate Brevibacillus reuszeri ? - ? 89 3.5.1.87 additional information the enzyme shows stereospecificity and a broad substrate specificity. No or poor activitz with N-carbamoyl-alpha-alanine, N-carbamoyl-glycine, N-carbamoyl-beta-alanine, and N-carbamoyl-alpha-aminoisobutyrate Brevibacillus reuszeri HSN1 ? - ? 89 3.5.1.87 additional information catalytic promiscuity of L-N-carbamoylase from Geobacillus stearothermophilus CECT43, substrate specificity with different N-formyl- and N-carbamoyl-DL-amino acids, overview. No N-formyl-DL-tert-leucine Geobacillus stearothermophilus CECT43 ? - ? 89 3.5.1.87 additional information the enzyme is a strict enantiospecific L-N-carbamoylase. Development of a bienzymatic system comprising an N-succinylamino acid racemase from Geobacillus kaustophilus CECT4264 and the enantiospecific L-N-carbamoylase from Geobacillus stearothermophilus CECT43. The biocatalyst system is able to produce optically pure natural and non-natural L-amino acids starting from racemic mixtures of N-acetyl-, N-formyl- and N-carbamoyl-amino acids by dynamic kinetic resolution. The fastest conversion rate is found with N-formyl-aminoacids, followed by N-carbamoyl- and N-acetyl-amino acids, and the an N-succinylamino acid racemase proves to be the limiting stepof the system due to its lower specific activity, overview Geobacillus stearothermophilus CECT43 ? - ? 89 3.5.1.87 N-acetyl-L-alanine + H2O - Geobacillus stearothermophilus L-alanine + acetate - ? 189720 3.5.1.87 N-acetyl-L-methionine + H2O - Sinorhizobium meliloti L-methionine + acetate - ? 389206 3.5.1.87 N-acetyl-L-methionine + H2O - Geobacillus stearothermophilus L-methionine + acetate - ? 389206 3.5.1.87 N-acetyl-L-phenylalanine + H2O - Geobacillus stearothermophilus L-phenylalanine + acetate - ? 189729 3.5.1.87 N-acetyl-L-tyrosine + H2O - Geobacillus stearothermophilus L-tyrosine + acetate - ? 414921 3.5.1.87 N-acetyl-L-valine + H2O - Geobacillus stearothermophilus L-valine + acetate - ? 189722 3.5.1.87 N-carbamoyl-D,L-serine + H2O - Brevibacillus reuszeri L-serine + CO2 + NH3 - ? 433989 3.5.1.87 N-carbamoyl-DL-2-aminoburyric acid + H2O - Geobacillus stearothermophilus L-2-aminobutyric acid + CO2 + NH3 - ? 433990 3.5.1.87 N-carbamoyl-DL-2-aminoburyric acid + H2O - Geobacillus stearothermophilus CECT43 L-2-aminobutyric acid + CO2 + NH3 - ? 433990 3.5.1.87 N-carbamoyl-DL-2-aminohexanoic acid + H2O 24% of activity with N-carbamoyl-DL-alanine Achromobacter xylosoxidans 2-aminohexanoic acid + CO2 + NH3 - ? 190010 3.5.1.87 N-carbamoyl-DL-alanine + H2O - Geobacillus stearothermophilus L-alanine + CO2 + NH3 - ? 189997 3.5.1.87 N-carbamoyl-DL-alanine + H2O - Pseudomonas sp. L-alanine + CO2 + NH3 - ? 189997 3.5.1.87 N-carbamoyl-DL-alanine + H2O - Achromobacter xylosoxidans L-alanine + CO2 + NH3 - ? 189997 3.5.1.87 N-carbamoyl-DL-alanine + H2O - Pseudomonas sp. NS671 L-alanine + CO2 + NH3 - ? 189997 3.5.1.87 N-carbamoyl-DL-aminobutyric acid + H2O - Geobacillus stearothermophilus L-aminobutyric acid + CO2 + NH3 - ? 433991 3.5.1.87 N-carbamoyl-DL-aspartic acid + H2O relative activity 7.81% Pseudomonas sp. DL-aspartic acid + CO2 + NH3 - ? 389251 3.5.1.87 N-carbamoyl-DL-cysteine + H2O relative activity 119% Pseudomonas sp. DL-cysteine + CO2 + NH3 - ? 389252 3.5.1.87 N-carbamoyl-DL-homophenylalanine + H2O - Geobacillus stearothermophilus L-homophenylalanine + CO2 + NH3 - ? 433992 3.5.1.87 N-carbamoyl-DL-homophenylalanine + H2O - Geobacillus stearothermophilus CECT43 L-homophenylalanine + CO2 + NH3 - ? 433992 3.5.1.87 N-carbamoyl-DL-methionine + H2O - Pseudomonas sp. L-methionine + CO2 + NH3 - ? 190011 3.5.1.87 N-carbamoyl-DL-methionine + H2O - Achromobacter xylosoxidans L-methionine + CO2 + NH3 - ? 190011 3.5.1.87 N-carbamoyl-DL-methionine + H2O - Geobacillus stearothermophilus L-methionine + CO2 + NH3 - ? 190011 3.5.1.87 N-carbamoyl-DL-methionine + H2O - Geobacillus stearothermophilus CECT43 L-methionine + CO2 + NH3 - ? 190011 3.5.1.87 N-carbamoyl-DL-norleucine + H2O - Geobacillus stearothermophilus L-norleucine + CO2 + NH3 - ? 433993 3.5.1.87 N-carbamoyl-DL-norvaline + H2O - Geobacillus stearothermophilus L-norvaline + CO2 + NH3 - ? 433994 3.5.1.87 N-carbamoyl-DL-phenylalanine + H2O - Pseudomonas sp. L-phenylalanine + CO2 + NH3 - ? 190005 3.5.1.87 N-carbamoyl-DL-phenylalanine + H2O 25% of activity with N-carbamoyl-DL-alanine Geobacillus stearothermophilus L-phenylalanine + CO2 + NH3 - ? 190005 3.5.1.87 N-carbamoyl-DL-phenylalanine + H2O 4.5% of activity with N-carbamoyl-L-alanine Achromobacter xylosoxidans L-phenylalanine + CO2 + NH3 - ? 190005 3.5.1.87 N-carbamoyl-DL-phenylalanine + H2O enzyme seems to be selective for aromatic amino acids Paenarthrobacter aurescens L-phenylalanine + CO2 + NH3 - ? 190005 3.5.1.87 N-carbamoyl-DL-phenylglycine + H2O low activity Geobacillus stearothermophilus L-phenylglycine + CO2 + NH3 - ? 433995 3.5.1.87 N-carbamoyl-DL-serine + H2O 19% of activity with N-carbamoyl-L-alanine Achromobacter xylosoxidans L-serine + CO2 + NH3 - ? 190001 3.5.1.87 N-carbamoyl-DL-serine + H2O 86% of activity with N-carbamoyl-DL-alanine Geobacillus stearothermophilus L-serine + CO2 + NH3 - ? 190001 3.5.1.87 N-carbamoyl-DL-serine + H2O relative activity 37.8% Pseudomonas sp. DL-serine + CO2 + NH3 - ? 389253 3.5.1.87 N-carbamoyl-DL-threonine + H2O 8.8% of activity with N-carbamoyl-L-alanine Achromobacter xylosoxidans L-threonine + CO2 + NH3 - ? 190002 3.5.1.87 N-carbamoyl-DL-threonine + H2O 94% of activity with N-carbamoyl-DL-alanine Geobacillus stearothermophilus L-threonine + CO2 + NH3 - ? 190002 3.5.1.87 N-carbamoyl-DL-valine + H2O - Geobacillus stearothermophilus L-valine + CO2 + NH3 - ? 189998 3.5.1.87 N-carbamoyl-DL-valine + H2O - Pseudomonas sp. L-valine + CO2 + NH3 - ? 189998 3.5.1.87 N-carbamoyl-DL-valine + H2O 28% of activity with N-carbamoyl-L-alanine Achromobacter xylosoxidans L-valine + CO2 + NH3 - ? 189998 3.5.1.87 N-carbamoyl-DL-valine + H2O - Pseudomonas sp. NS671 L-valine + CO2 + NH3 - ? 189998 3.5.1.87 N-carbamoyl-glycine + H2O - Pseudomonas sp. ON4a glycine + CO2 + NH3 - ? 189996 3.5.1.87 N-carbamoyl-glycine + H2O 75% of activity with N-carbamoyl-L-alanine Achromobacter xylosoxidans glycine + CO2 + NH3 - ? 189996 3.5.1.87 N-carbamoyl-glycine + H2O 71% of activity with N-carbamoyl-DL-alanine Geobacillus stearothermophilus glycine + CO2 + NH3 - ? 189996 3.5.1.87 N-carbamoyl-glycine + H2O relative activity 72.4% Pseudomonas sp. glycine + CO2 + NH3 - ? 189996 3.5.1.87 N-carbamoyl-L-alanine + H2O - Sinorhizobium meliloti L-alanine + CO2 + NH3 - ? 389254 3.5.1.87 N-carbamoyl-L-alanine + H2O - Geobacillus stearothermophilus L-alanine + CO2 + NH3 - ? 389254 3.5.1.87 N-carbamoyl-L-alanine + H2O - Achromobacter xylosoxidans L-alanine + CO2 + NH3 - ? 389254 3.5.1.87 N-carbamoyl-L-alanine + H2O - Pseudomonas sp. ON4a L-alanine + CO2 + NH3 - ? 389254 3.5.1.87 N-carbamoyl-L-alanine + H2O relative activity 41.2% Pseudomonas sp. L-alanine + CO2 + NH3 - ? 389254 3.5.1.87 N-carbamoyl-L-asparagine + H2O 64% of activity with N-carbamoyl-L-alanine Achromobacter xylosoxidans L-asparagine + CO2 + NH3 - ? 190004 3.5.1.87 N-carbamoyl-L-asparagine + H2O 52% of activity with N-carbamoyl-DL-alanine Geobacillus stearothermophilus L-asparagine + CO2 + NH3 - ? 190004 3.5.1.87 N-carbamoyl-L-cysteine + H2O - Sinorhizobium meliloti L-cysteine + CO2 + NH3 - ? 386589 3.5.1.87 N-carbamoyl-L-cysteine + H2O - Pseudomonas sp. L-cysteine + CO2 + NH3 - ? 386589 3.5.1.87 N-carbamoyl-L-cysteine + H2O - Pseudomonas sp. ON4a L-cysteine + CO2 + NH3 - ? 386589 3.5.1.87 N-carbamoyl-L-cysteine + H2O relative activity 100% Pseudomonas sp. L-cysteine + CO2 + NH3 - ? 386589 3.5.1.87 N-carbamoyl-L-cysteine + H2O - Pseudomonas sp. QR-101 L-cysteine + CO2 + NH3 - ? 386589 3.5.1.87 N-carbamoyl-L-glutamic acid + H2O - Sinorhizobium meliloti L-glutamic acid + CO2 + NH3 - ? 190003 3.5.1.87 N-carbamoyl-L-glutamic acid + H2O 56% of activity with N-carbamoyl-DL-alanine Geobacillus stearothermophilus L-glutamic acid + CO2 + NH3 - ? 190003 3.5.1.87 N-carbamoyl-L-homophenylalanine + H2O - Geobacillus kaustophilus L-homophenylalanine + NH3 + CO2 - ? 454426 3.5.1.87 N-carbamoyl-L-isoleucine + H2O - Pseudomonas sp. L-isoleucine + CO2 + NH3 - ? 190000 3.5.1.87 N-carbamoyl-L-isoleucine + H2O 5.3% of activity with N-carbamoyl-L-alanine Achromobacter xylosoxidans L-isoleucine + CO2 + NH3 - ? 190000 3.5.1.87 N-carbamoyl-L-isoleucine + H2O 55% of activity with N-carbamoyl-DL-alanine Geobacillus stearothermophilus L-isoleucine + CO2 + NH3 - ? 190000 3.5.1.87 N-carbamoyl-L-isoleucine + H2O - Pseudomonas sp. NS671 L-isoleucine + CO2 + NH3 - ? 190000 3.5.1.87 N-carbamoyl-L-leucine + H2O - Pseudomonas sp. L-leucine + CO2 + NH3 - ? 189999 3.5.1.87 N-carbamoyl-L-leucine + H2O - Achromobacter xylosoxidans L-leucine + CO2 + NH3 - ? 189999 3.5.1.87 N-carbamoyl-L-leucine + H2O 9.1% of activity with N-carbamoyl-L-alanine Geobacillus stearothermophilus L-leucine + CO2 + NH3 - ? 189999 3.5.1.87 N-carbamoyl-L-leucine + H2O - Pseudomonas sp. NS671 L-leucine + CO2 + NH3 - ? 189999 3.5.1.87 N-carbamoyl-L-methionine + H2O - Geobacillus stearothermophilus L-methionine + CO2 + NH3 - ? 189995 3.5.1.87 N-carbamoyl-L-methionine + H2O - Pseudomonas sp. L-methionine + CO2 + NH3 - ? 189995 3.5.1.87 N-carbamoyl-L-methionine + H2O - Pseudomonas sp. ON4a L-methionine + CO2 + NH3 - ? 189995 3.5.1.87 N-carbamoyl-L-methionine + H2O - Brevibacillus reuszeri L-methionine + CO2 + NH3 - ? 189995 3.5.1.87 N-carbamoyl-L-methionine + H2O preferred substrate Brevibacillus reuszeri L-methionine + CO2 + NH3 - ? 189995 3.5.1.87 N-carbamoyl-L-methionine + H2O 12% of activity with N-carbamoyl-L-alanine Achromobacter xylosoxidans L-methionine + CO2 + NH3 - ? 189995 3.5.1.87 N-carbamoyl-L-methionine + H2O L-stereospecific enzyme Geobacillus stearothermophilus L-methionine + CO2 + NH3 - ? 189995 3.5.1.87 N-carbamoyl-L-methionine + H2O L-stereospecific enzyme Pseudomonas sp. L-methionine + CO2 + NH3 - ? 189995 3.5.1.87 N-carbamoyl-L-methionine + H2O L-stereospecific enzyme Achromobacter xylosoxidans L-methionine + CO2 + NH3 - ? 189995 3.5.1.87 N-carbamoyl-L-methionine + H2O L-stereospecific enzyme Paenarthrobacter aurescens L-methionine + CO2 + NH3 - ? 189995 3.5.1.87 N-carbamoyl-L-methionine + H2O activity assay Sinorhizobium meliloti L-methionine + CO2 + NH3 - ? 189995 3.5.1.87 N-carbamoyl-L-methionine + H2O kinetic assay Sinorhizobium meliloti L-methionine + CO2 + NH3 - ? 189995 3.5.1.87 N-carbamoyl-L-methionine + H2O relative activity 44.4% Pseudomonas sp. L-methionine + CO2 + NH3 - ? 189995 3.5.1.87 N-carbamoyl-L-methionine + H2O preferred substrate Brevibacillus reuszeri HSN1 L-methionine + CO2 + NH3 - ? 189995 3.5.1.87 N-carbamoyl-L-methionine + H2O - Brevibacillus reuszeri HSN1 L-methionine + CO2 + NH3 - ? 189995 3.5.1.87 N-carbamoyl-L-methionine + H2O L-stereospecific enzyme Pseudomonas sp. NS671 L-methionine + CO2 + NH3 - ? 189995 3.5.1.87 N-carbamoyl-L-methionine + H2O - Pseudomonas sp. NS671 L-methionine + CO2 + NH3 - ? 189995 3.5.1.87 N-carbamoyl-L-phenylalanine + H2O - Sinorhizobium meliloti L-phenylalanine + CO2 + NH3 - ? 389256 3.5.1.87 N-carbamoyl-L-phenylalanine + H2O - Geobacillus stearothermophilus L-phenylalanine + CO2 + NH3 - ? 389256 3.5.1.87 N-carbamoyl-L-phenylalanine + H2O - Achromobacter xylosoxidans L-phenylalanine + CO2 + NH3 - ? 389256 3.5.1.87 N-carbamoyl-L-phenylalanine + H2O - Brevibacillus reuszeri L-phenylalanine + CO2 + NH3 - ? 389256 3.5.1.87 N-carbamoyl-L-phenylalanine + H2O the recombinant L-carbamoylase from Escherichia coli harboring the pET-Case is L-stereospecific Siminovitchia fordii L-phenylalanine + CO2 + NH3 - ? 389256 3.5.1.87 N-carbamoyl-L-phenylalanine + H2O - Brevibacillus reuszeri HSN1 L-phenylalanine + CO2 + NH3 - ? 389256 3.5.1.87 N-carbamoyl-L-phenylglycine + H2O - Brevibacillus reuszeri L-phenylglycine + CO2 + NH3 - ? 433996 3.5.1.87 N-carbamoyl-L-thienylalanine + H2O - Paenarthrobacter aurescens L-thienylalanine + CO2 + NH3 - ? 190014 3.5.1.87 N-carbamoyl-L-tryptophan + H2O - Sinorhizobium meliloti L-tryptophan + CO2 + NH3 - ? 190012 3.5.1.87 N-carbamoyl-L-tryptophan + H2O - Paenarthrobacter aurescens L-tryptophan + CO2 + NH3 - ? 190012 3.5.1.87 N-carbamoyl-L-tryptophan + H2O - Brevibacillus reuszeri L-tryptophan + CO2 + NH3 - ? 190012 3.5.1.87 N-carbamoyl-L-tryptophan + H2O - Brevibacillus reuszeri HSN1 L-tryptophan + CO2 + NH3 - ? 190012 3.5.1.87 N-carbamoyl-L-tyrosine + H2O - Sinorhizobium meliloti L-tyrosine + CO2 + NH3 - ? 190006 3.5.1.87 N-carbamoyl-L-tyrosine + H2O - Geobacillus stearothermophilus L-tyrosine + CO2 + NH3 - ? 190006 3.5.1.87 N-carbamoyl-L-tyrosine + H2O - Pseudomonas sp. L-tyrosine + CO2 + NH3 - ? 190006 3.5.1.87 N-carbamoyl-L-tyrosine + H2O - Paenarthrobacter aurescens L-tyrosine + CO2 + NH3 - ? 190006 3.5.1.87 N-carbamoyl-L-tyrosine + H2O 3% of activity with N-carbamoyl-DL-alanine Geobacillus stearothermophilus L-tyrosine + CO2 + NH3 - ? 190006 3.5.1.87 N-carbamoyl-L-valine + H2O - Sinorhizobium meliloti L-valine + CO2 + NH3 - ? 389257 3.5.1.87 N-carbamoyl-L-valine + H2O - Geobacillus stearothermophilus L-valine + CO2 + NH3 - ? 389257 3.5.1.87 N-carbamoyl-L-valine + H2O - Achromobacter xylosoxidans L-valine + CO2 + NH3 - ? 389257 3.5.1.87 N-carbamoyl-L-valine + H2O - Brevibacillus reuszeri L-valine + CO2 + NH3 - ? 389257 3.5.1.87 N-carbamoyl-L-valine + H2O - Brevibacillus reuszeri HSN1 L-valine + CO2 + NH3 - ? 389257 3.5.1.87 N-formyl-DL-2-aminobutyric acid + H2O - Geobacillus stearothermophilus L-2-aminobutyric acid + CO2 - ? 434002 3.5.1.87 N-formyl-DL-alanine + H2O - Geobacillus stearothermophilus L-alanine + CO2 - ? 190007 3.5.1.87 N-formyl-DL-alanine + H2O 13% of activity with N-carbamoyl-DL-alanine Achromobacter xylosoxidans L-alanine + CO2 - ? 190007 3.5.1.87 N-formyl-DL-alanine + H2O - Geobacillus stearothermophilus CECT43 L-alanine + CO2 - ? 190007 3.5.1.87 N-formyl-DL-aminobutyric acid + H2O - Geobacillus stearothermophilus L-aminobutyric acid + CO2 - ? 434003 3.5.1.87 N-formyl-DL-ethionine + H2O - Geobacillus stearothermophilus L-ethionine + CO2 - ? 434004 3.5.1.87 N-formyl-DL-homophenylalanine + H2O - Geobacillus stearothermophilus L-homophenylalanine + CO2 - ? 434005 3.5.1.87 N-formyl-DL-leucine + H2O 5.2% of activity with N-carbamoyl-DL-alanine Achromobacter xylosoxidans L-leucine + CO2 - ? 190008 3.5.1.87 N-formyl-DL-methionine + H2O - Geobacillus stearothermophilus L-methionine + CO2 - ? 190009 3.5.1.87 N-formyl-DL-methionine + H2O 5.4% of activity with N-carbamoyl-DL-alanine Achromobacter xylosoxidans L-methionine + CO2 - ? 190009 3.5.1.87 N-formyl-DL-methionine + H2O - Geobacillus stearothermophilus CECT43 L-methionine + CO2 - ? 190009 3.5.1.87 N-formyl-DL-norleucine + H2O - Geobacillus stearothermophilus L-norleucine + CO2 - ? 434006 3.5.1.87 N-formyl-DL-norvaline + H2O - Geobacillus stearothermophilus L-norvaline + CO2 - ? 434007 3.5.1.87 N-formyl-DL-phenylalanine + H2O - Geobacillus stearothermophilus L-phenylalanine + CO2 - ? 434008 3.5.1.87 N-formyl-DL-phenylglycine + H2O - Geobacillus stearothermophilus L-phenylglycine + CO2 - ? 434009 3.5.1.87 N-formyl-DL-tryptophan + H2O - Paenarthrobacter aurescens L-tryptophan + CO2 - ? 190013 3.5.1.87 N-formyl-DL-tryptophan + H2O - Geobacillus stearothermophilus L-tryptophan + CO2 - ? 190013 3.5.1.87 N-formyl-DL-tryptophan + H2O - Geobacillus stearothermophilus CECT43 L-tryptophan + CO2 - ? 190013 3.5.1.87 N-formyl-L-alanine + H2O - Geobacillus stearothermophilus L-alanine + CO2 - ? 414950 3.5.1.87 N-formyl-L-methionine + H2O - Sinorhizobium meliloti L-methionine + HCOOH - ? 389267 3.5.1.87 N-formyl-L-methionine + H2O - Geobacillus stearothermophilus L-methionine + HCOOH - ? 389267 3.5.1.87 N-formyl-L-phenylalanine + H2O - Geobacillus stearothermophilus L-phenylalanine + CO2 - ? 414954 3.5.1.87 N-formyl-L-tyrosine + H2O - Geobacillus stearothermophilus L-tyrosine + CO2 - ? 414955 3.5.1.87 N-formyl-L-valine + H2O - Geobacillus stearothermophilus L-valine + CO2 - ? 414956