3.5.1.23 (2R,3Z)-2-([(2E)-1-hydroxy-12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodec-2-en-1-yl]amino)heptadec-3-ene-1,3-diol + H2O 9.9% hydrolysis Homo sapiens ? - ir 432993 3.5.1.23 (4E,2S,3R)-2-N-(10-pyrenedecanoyl)-1,3,17-trihydroxy-17-(3,5-dinitrobenzoyl)-4-heptadecene + H2O - Pseudomonas aeruginosa ? fluorescent product formed upon hydrolysis of the N-acyl bond ? 380798 3.5.1.23 11-eicosenoyl-ceramide + H2O - Homo sapiens sphingosine + 11-eicosenoic acid - ? 413846 3.5.1.23 11-eicosenoyl-dihydroceramide + H2O - Homo sapiens dihydrosphingosine + 11-eicosenoic acid - ? 413847 3.5.1.23 11-eicosenoyl-phytoceramide + H2O - Homo sapiens phytosphingosine + 11-eicosenoic acid - ? 413848 3.5.1.23 11-[[9-(diethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]-N-[(2S,3R,4E)-1,3-dihydroxy-7-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]hept-4-en-2-yl]undecanamide + H2O - Homo sapiens ? - ir 433119 3.5.1.23 11-[[9-(diethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]-N-[(2S,3R,4E)-1,3-dihydroxy-7-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]hept-4-en-2-yl]undecanamide + H2O - Mus musculus ? - ir 433119 3.5.1.23 12:0-ceramide + H2O - Danio rerio sphingosine + laurate - ? 449167 3.5.1.23 4-nitrobenzo-2-oxa-1,3-diazole-C12-ceramide + H2O - Mus musculus 4-nitrobenzo-2-oxa-1,3-diazole-dodecanoic acid + ceramide - ? 383913 3.5.1.23 4-nitrobenzo-2-oxa-1,3-diazole-C12-ceramide + H2O - Danio rerio 4-nitrobenzo-2-oxa-1,3-diazole-dodecanoic acid + ceramide - ? 383913 3.5.1.23 4-nitrobenzo-2-oxa-1,3-diazole-C12-ceramide + H2O - Homo sapiens 4-nitrobenzo-2-oxa-1,3-diazole-dodecanoic acid + ceramide - ? 383913 3.5.1.23 4-nitrobenzo-2-oxa-1,3-diazole-ceramide + H2O C12 substrate chain length Pseudomonas aeruginosa ? - ? 390889 3.5.1.23 4-nitrobenzo-2-oxa-1,3-diazole-ceramide + H2O C12 substrate chain length Pseudomonas aeruginosa AN17 ? - ? 390889 3.5.1.23 4-nitrobenzo-2-oxa-1,3-diazole-N-dodecanoylsphingosine + H2O - Mus musculus ? - ? 116237 3.5.1.23 4-nitrobenzo-2-oxa-1,3-diazole-N-hexanoylsphingosine + H2O - Mus musculus ? - ? 116238 3.5.1.23 4-nitrobenzo-2-oxa-1,3-diazoyl-N-dodecanoylsphingosine + H2O - Rattus norvegicus 4-nitrobenzo-2-oxa-1,3-diazoyl-dodecanoic acid + sphingosine - r 372045 3.5.1.23 4-nitrobenzo-2-oxa-1,3-diazoyl-N-dodecanoylsphingosine + H2O much faster hydrolysis than of N-lauroylsphingosine Rattus norvegicus 4-nitrobenzo-2-oxa-1,3-diazoyl-dodecanoic acid + sphingosine - ? 372045 3.5.1.23 7-nitrobenz-2-oxa-1,3-diazole-N-lauroylsphingosine + H2O - Pseudomonas aeruginosa ? - r 116239 3.5.1.23 C12-4-nitrobenzo-2-oxa-1,3-diazole-ceramide + H2O - Mus musculus ? - ? 116232 3.5.1.23 C12-4-nitrobenzo-2-oxa-1,3-diazole-ceramide + H2O substrate activity assay Pseudomonas aeruginosa ? - ? 116232 3.5.1.23 C12-4-nitrobenzo-2-oxa-1,3-diazole-ceramide + H2O substrate activity assay Rattus norvegicus ? - ? 116232 3.5.1.23 C12-4-nitrobenzo-2-oxa-1,3-diazole-ceramide + H2O substrate activity assay Drosophila melanogaster ? - ? 116232 3.5.1.23 C12-NBD-Cer + H2O the enzyme catalyzes also the reverse reaction of ceramide synthesis Camelus dromedarius NBD-dodecanoic acid + D-erythro-sphingosine - r 433528 3.5.1.23 C12-NBD-Cer + H2O - Arabidopsis thaliana NBD-dodecanoic acid + sphingosine - ? 433529 3.5.1.23 C12-NBD-Cer + H2O - Arabidopsis thaliana Landsberg erecta NBD-dodecanoic acid + sphingosine - ? 433529 3.5.1.23 C12:0-ceramide + H2O - Homo sapiens laurate + sphingosine - ? 413302 3.5.1.23 C12:0-ceramide + H2O preferred substrate Nilaparvata lugens laurate + sphingosine - ? 413302 3.5.1.23 C14:0-ceramide + H2O - Homo sapiens myristate + sphingosine - ? 413303 3.5.1.23 C16-14C-ceramide + H2O - Mus musculus ? - ? 453335 3.5.1.23 C16:0-ceramide + H2O - Homo sapiens palmitate + sphingosine - ? 413304 3.5.1.23 C16:0-ceramide + H2O low activity Nilaparvata lugens palmitate + sphingosine - ? 413304 3.5.1.23 C18-ceramide + H2O preferred substrate, fluorescent C18 ceramide in detergent micelles Homo sapiens stearate + sphingosine - ? 449723 3.5.1.23 C18:0-ceramide + H2O - Homo sapiens stearate + sphingosine - ? 413305 3.5.1.23 C18:1-ceramide + H2O - Homo sapiens oleate + sphingosine - ? 413306 3.5.1.23 C20:0-ceramide + H2O - Homo sapiens eicosanoic acid + sphingosine - ? 413307 3.5.1.23 C20:0-ceramide + H2O low activity Nilaparvata lugens arachidic acid + sphingosine - ? 449724 3.5.1.23 C20:1-ceramide + H2O - Homo sapiens C20:1 fatty acid + sphingosine - ? 413308 3.5.1.23 C24-ceramide + H2O - Aspergillus oryzae RIB40 C24 fatty acid + sphingosine - r 414404 3.5.1.23 C24-ceramide + H2O - Homo sapiens lignocerate + sphingosine - ? 449726 3.5.1.23 C24:0-ceramide + H2O - Homo sapiens C24:0 fatty acid + sphingosine - ? 413309 3.5.1.23 C24:0-ceramide + H2O low activity Nilaparvata lugens lignoceric acid + sphingosine - ? 449727 3.5.1.23 C24:1-ceramide + H2O - Homo sapiens C24:1 fatty acid + sphingosine - ? 414405 3.5.1.23 C2:0-ceramide + H2O low activity Nilaparvata lugens acetate + sphingosine - ? 449728 3.5.1.23 C5-ceramide + H2O - Mus musculus valerate + sphingosine - ? 449731 3.5.1.23 C6-ceramide + H2O - Homo sapiens hexanoate + sphingosine - ? 449732 3.5.1.23 C6:0-ceramide + H2O - Homo sapiens hexanoate + sphingosine - ? 413310 3.5.1.23 C6:0-ceramide + H2O - Nilaparvata lugens hexanoate + sphingosine - ? 413310 3.5.1.23 ceramide + H2O - Drosophila melanogaster sphingosine + fatty acid - ? 116225 3.5.1.23 ceramide + H2O - Mus musculus sphingosine + fatty acid - ? 116225 3.5.1.23 ceramide + H2O - Mus musculus sphingosine + fatty acid - r 116225 3.5.1.23 ceramide + H2O - Homo sapiens sphingosine + fatty acid - ? 116225 3.5.1.23 ceramide + H2O - Homo sapiens sphingosine + fatty acid - r 116225 3.5.1.23 ceramide + H2O - Rattus norvegicus sphingosine + fatty acid - ? 116225 3.5.1.23 ceramide + H2O - Rattus norvegicus sphingosine + fatty acid - r 116225 3.5.1.23 ceramide + H2O - Mycobacterium tuberculosis sphingosine + fatty acid - r 116225 3.5.1.23 ceramide + H2O - Oryza sativa sphingosine + fatty acid - ? 116225 3.5.1.23 ceramide + H2O C16-ceramide Rattus norvegicus sphingosine + fatty acid - ? 116225 3.5.1.23 ceramide + H2O for ceramide clearance, ceramidases hydrolyze the N-acyl fatty acid, yielding in the generation of sphingosine Homo sapiens sphingosine + fatty acid - ? 116225 3.5.1.23 ceramide + H2O ceramidase activity on D-e-C6, D-e-C12, D-e-C16, D-e-C18, and D-e-C24:1-ceramide Drosophila melanogaster sphingosine + fatty acid - ? 116225 3.5.1.23 ceramide + H2O the recombinant enzyme shows a broad subsrate specificity with ceramides, it shows good activity with ceramides containing C6-C24 fatty acids, and preference of ceramides with monounsaturated fatty acids than saturated fatty acids, overview Mycobacterium tuberculosis sphingosine + fatty acid - r 116225 3.5.1.23 ceramide + H2O - Drosophila melanogaster BL-18012 sphingosine + fatty acid - ? 116225 3.5.1.23 ceramide + H2O ceramidase activity on D-e-C6, D-e-C12, D-e-C16, D-e-C18, and D-e-C24:1-ceramide Drosophila melanogaster BL-18012 sphingosine + fatty acid - ? 116225 3.5.1.23 ceramide + H2O substrate with about 30% activity of GM2 Pseudomonas sp. ? - ? 400643 3.5.1.23 ceramide + H2O ceramide in anionic liposomes. The negatively charged liposomes consisted of 25% mole bis(monoacylglycero)phosphate, 45% 1,2-dimyristoyl-sn-glycero-3-phosphocholine, 20% cholesterol, and 10% 12:0 ceramide Homo sapiens ? - ? 400643 3.5.1.23 ceramide + H2O - Rattus norvegicus sphingosine + a fatty acid - ? 413316 3.5.1.23 ceramide + H2O - Homo sapiens sphingosine + a fatty acid - ? 413316 3.5.1.23 ceramide + H2O - Danio rerio sphingosine + a fatty acid - ? 413316 3.5.1.23 ceramide + H2O - Rattus norvegicus sphingosine + a fatty acid sphongosine is an apoptosis mediator ? 413316 3.5.1.23 ceramide + H2O - Homo sapiens carboxylate + sphingosine - ? 432736 3.5.1.23 ceramide + H2O - Homo sapiens fatty acid + sphingosine - ? 448501 3.5.1.23 D-erythro-4-nitrobenzo-2-oxa-1,3-diazole-C12-ceramide + H2O - Rattus norvegicus D-erythro-4-nitrobenzo-2-oxa-1,3-diazole-dodecanoic acid + ceramide - ? 384518 3.5.1.23 D-erythro-4-nitrobenzo-2-oxa-1,3-diazole-C12-ceramide + H2O - Homo sapiens D-erythro-4-nitrobenzo-2-oxa-1,3-diazole-dodecanoic acid + ceramide - ? 384518 3.5.1.23 D-erythro-C12-4-nitrobenzo-2-oxa-1,3-diazole-ceramide + H2O - Homo sapiens D-erythro-sphingosine + ? - ? 404788 3.5.1.23 D-erythro-C12-4-nitrobenzo-2-oxa-1,3-diazole-ceramide + H2O substrate activity assay Oryza sativa D-erythro-sphingosine + ? - ? 404788 3.5.1.23 D-erythro-C12-4-nitrobenzo-2-oxa-1,3-diazole-phytoceramide + H2O substrate activity assay Oryza sativa D-erythro-sphingosine + ? - ? 404789 3.5.1.23 D-erythro-C12-NBD-ceramide + H2O maCER1 has a highly restricted substrate specificity, maCER1 has a major ceramidase activity and only a very minor reverse activity Mus musculus D-erythro-sphingosine + 4-nitrobenzo-2-oxa-1,3-diazoyl-dodecanoic acid - r 372835 3.5.1.23 D-erythro-C12-NBD-ceramide + H2O substrate in activity assay Mus musculus D-erythro-sphingosine + 4-nitrobenzo-2-oxa-1,3-diazoyl-dodecanoic acid - ? 372835 3.5.1.23 D-erythro-C24:1-ceramide + H2O - Homo sapiens C24:1 fatty acid + D-erythro-sphingosine - ? 413342 3.5.1.23 D-erythro-dihydrosphingosine + fatty acid very low ceramide synthesis activity Rattus norvegicus ? - ? 372836 3.5.1.23 D-erythro-dodecanoyl-7-nitrobenz-2-oxa-1,3-diazol-4-yl-ceramide + H2O - Mus musculus 12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodecanoic acid + sphingosine - r 420522 3.5.1.23 D-erythro-dodecanoyl-7-nitrobenz-2-oxa-1,3-diazol-4-yl-ceramide + H2O - Rattus norvegicus 12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodecanoic acid + sphingosine - r 420522 3.5.1.23 D-erythro-myristoyl-sphingosine + H2O - Homo sapiens myristate + sphingosine - ? 383115 3.5.1.23 D-erythro-octadecanoyl-ceramide + H2O mtCDase specifically hydrolyzes the D-erythro-isomer of ceramide, requirements for ceramide-enzyme interaction, ligand interaction with the enzyme occurs in a high affinity low specificity manner, in contrast to catalysis which is highly specific for D-erythro-ceramide but occurs with a lower affinity Rattus norvegicus D-erythro-sphingosine + palmitic acid - ? 372837 3.5.1.23 D-erythro-oleoyl-sphingosine + H2O - Homo sapiens oleate + sphingosine - ? 383116 3.5.1.23 D-erythro-palmitoyl-sphingosine + H2O - Homo sapiens palmitate + sphingosine - ? 383117 3.5.1.23 D-erythro-sphingosine + (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexa-4,7,10,13,16,19-enoic acid 50% of activity Pseudomonas aeruginosa N-((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexa-4,7,10,13,16,19-enoyl)-D-erythro-sphingosine + H2O - r 381730 3.5.1.23 D-erythro-sphingosine + arachidonic acid 61% of activity Pseudomonas aeruginosa N-arachidonyl-D-erythro-sphingosine + H2O - r 381727 3.5.1.23 D-erythro-sphingosine + behenic acid 24% of activity Pseudomonas aeruginosa N-behenoyl-D-erythro-sphingosine + H2O - r 381728 3.5.1.23 D-erythro-sphingosine + cerotic acid 4% of activity Pseudomonas aeruginosa N-cerotoyl-D-erythro-sphingosine + H2O - r 381729 3.5.1.23 D-erythro-sphingosine + eicosapentaenoic acid 52% of activity Pseudomonas aeruginosa N-eicosapentaenyl-D-erythro-sphingosine + H2O - r 381731 3.5.1.23 D-erythro-sphingosine + fatty acid highest ceramide synthesis activity with D-erythro-sphingosine Rattus norvegicus ? - ? 372838 3.5.1.23 D-erythro-sphingosine + fatty acid - Pseudomonas aeruginosa N-acyl-D-erythro-sphingosine + H2O - r 381732 3.5.1.23 D-erythro-sphingosine + lauric acid 72% of activity Pseudomonas aeruginosa N-lauroyl-D-erythro-sphingosine + H2O - r 381733 3.5.1.23 D-erythro-sphingosine + lignoceric acid 6% of activity Pseudomonas aeruginosa N-lignoceroyl-D-erythro-sphingosine + H2O - r 381734 3.5.1.23 D-erythro-sphingosine + linoleic acid 66% of activity Pseudomonas aeruginosa N-linoleoyl-D-erythro-sphingosine + H2O - r 381735 3.5.1.23 D-erythro-sphingosine + myristic acid best substrate Pseudomonas aeruginosa N-myristoyl-D-erythro-sphingosine + H2O - r 381736 3.5.1.23 D-erythro-sphingosine + oleic acid 39% of activity Pseudomonas aeruginosa N-oleoyl-D-erythro-sphingosine + H2O - r 381737 3.5.1.23 D-erythro-sphingosine + palmitic acid 70% of activity, palmitic acid can not be replaced with palmityl-CoA Pseudomonas aeruginosa N-palmitoyl-D-erythro-sphingosine + H2O - r 381738 3.5.1.23 D-erythro-sphingosine + stearic acid 49% of activity Pseudomonas aeruginosa N-stearoyl-D-erythro-sphingosine + H2O - r 381739 3.5.1.23 D-erythro-tetracosanoyl-sphingosine + H2O i.e. D-e-C24:0-ceramide Homo sapiens tetracosanoate + sphingosine - ? 383114 3.5.1.23 D-threo-sphingosine + fatty acid low ceramide synthesis activity Rattus norvegicus ? - ? 372978 3.5.1.23 dihydro-C16-ceramide + H2O - Rattus norvegicus ? - ? 116231 3.5.1.23 dihydro-N-myristoyl-D-erythro-sphingosine + H2O 55.9% hydrolysis compared to N-palmitoyl-D-erythro-sphingosine Rattus norvegicus dihydro-D-erythro-sphingosine + myristate - ? 420599 3.5.1.23 dihydro-N-palmitoyl-D-erythro-sphingosine + H2O 4.5% hydrolysis compared to N-palmitoyl-D-erythro-sphingosine Rattus norvegicus dihydro-D-erythro-sphingosine + palmitate - ? 420600 3.5.1.23 dihydroceramide + H2O - Homo sapiens dihydrosphingosine + fatty acid - ? 413350 3.5.1.23 ganglioside + H2O GM1, GM2, GM3 Nocardia sp. lyso-ganglioside + fatty acid corresponding to ganglioside type ? 116224 3.5.1.23 ganglioside GM2 + H2O sphingolipid, best substrate Pseudomonas sp. ? - ? 115518 3.5.1.23 hexadecanoyl-ceramide + H2O i.e. C16-ceramide Aspergillus oryzae RIB40 hexadecanoate + sphingosine - r 414694 3.5.1.23 L-erythro-sphingosine + fatty acid low ceramide synthesis activity Rattus norvegicus ? - ? 373577 3.5.1.23 L-threo-sphingosine + fatty acid low ceramide synthesis activity Rattus norvegicus ? - ? 373640 3.5.1.23 linoleoylsphingosine + H2O - Cavia porcellus linoleic acid + sphingosine - ? 116245 3.5.1.23 additional information broad substrate specificity Pseudomonas sp. ? - ? 89 3.5.1.23 additional information CDase may be a virulence factor Pseudomonas aeruginosa ? - ? 89 3.5.1.23 additional information critical role of AC in the regulation of sphingolipid metabolism Homo sapiens ? - ? 89 3.5.1.23 additional information maCER1 may play a role in regulating the levels of bioactive lipids ceramide and sphingosine-1-phosphate, as well as complex sphingolipids, maCER1 may have a specific role in skin function Mus musculus ? - ? 89 3.5.1.23 additional information neutral ceramidase is involved in the regulation of ceramide-mediated signaling Rattus norvegicus ? - ? 89 3.5.1.23 additional information no substrate in the ceramide synthesis reaction: palmitoyl-CoA Rattus norvegicus ? - ? 89 3.5.1.23 additional information not: D-erythro-C12-NBD-dihydroceramide, D-ribo-C12-NBD-phytoceramide, L-threo-C12-NBD-ceramide Mus musculus ? - ? 89 3.5.1.23 additional information not: glycosphingolipids, e.g. GalCer, sulfatide, Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1’)ceramide, sphingomyelin Rattus norvegicus ? - ? 89 3.5.1.23 additional information the C-terminal residues Ile-758 and Phe-756 are essential for enzyme function, the C-terminal tail is indispensable for the correct folding, localization and enzyme activity Rattus norvegicus ? - ? 89 3.5.1.23 additional information the C-terminal tail is essential for enzyme activity and correct folding Pseudomonas aeruginosa ? - ? 89 3.5.1.23 additional information acid ceramidase, but not acid sphingomyelinase, is required for tumor necrosis factor-alpha-induced prostaglandin PGE2 production, overview Mus musculus ? - ? 89 3.5.1.23 additional information acidic and alkaline isozyme activities increase with keratinocyte differentiation, while the activities of phytoalkaline and neutral isozymes decrease, overview Homo sapiens ? - ? 89 3.5.1.23 additional information CER2 catalyzes the hydrolysis of ceramides derived from both the de novo and sphingomyelin breakdown pathways, Golgi alkaline ceramidase regulates cell proliferation and survival by controlling levels of sphingosine and sphingosine 1-phosphate Homo sapiens ? - ? 89 3.5.1.23 additional information cooperative prosurvival activity by MAP kinases ERK and Akt in human alveolar macrophages is dependent on high levels of acid ceramidase activity, overview, blocking of acid ceramidase but not sphingosine kinase activity in alveolar macrophages leads to decreased ERK and Akt activity and induction of cell death, sphingosine and L-threo-dihydrosphingosine reverse the antisurvival effects of acid ceramidase inhibition in alveolar macrophages Homo sapiens ? - ? 89 3.5.1.23 additional information the enzyme is important in digestion of sphingolipids Rattus norvegicus ? - ? 89 3.5.1.23 additional information the enzyme is involved in ceramide metabolism at the plasma membrane and in extracellular milieu, hydrolysis of ceramide on the cell surface, overview, involvement of the enzyme in stimulus-induced Cer metabolism by doxorubicin Mus musculus ? - ? 89 3.5.1.23 additional information the enzyme is involved in degradation of epidermal ceramides, ceramidase activity is not age-dependent Mus musculus ? - ? 89 3.5.1.23 additional information diacylglycerol assay, i.e. ceramide:DAG assay Homo sapiens ? - ? 89 3.5.1.23 additional information ceramidase enhances phospholipase C-induced hemolysis by Pseudomonas aeruginosa Pseudomonas aeruginosa ? - ? 89 3.5.1.23 additional information acid ceramidase plays a critical role in the regulation of ceramide and sphingosine metabolism Homo sapiens ? - ? 89 3.5.1.23 additional information ASAH1 hydrolyzes ceramides and regulates neuronal development, and its deficiency often results in mental retardation. Results of linkage disequilibrium and polymorphism analyses suggest that a particular group of haplotypes in ASAH1 represents a signature of recent positive Darwinian selection Homo sapiens ? - ? 89 3.5.1.23 additional information ceramidase enhances cytotoxicity induced by hemolytic phospholipase C Pseudomonas aeruginosa ? - ? 89 3.5.1.23 additional information neutral ceramidase is a key enzyme for hydrolysis of sphingomyelin in the gut Rattus norvegicus ? - ? 89 3.5.1.23 additional information neutral ceramidase plays a major role in ceramide metabolism in the human gut Homo sapiens ? - ? 89 3.5.1.23 additional information acid ceramidase deacylates ceramide and yields sphingosine Homo sapiens ? - ? 89 3.5.1.23 additional information ceramidase catalyzes the hydrolysis of ceramide into sphingosine and a free fatty acid Homo sapiens ? - ? 89 3.5.1.23 additional information ceramidase catalyzes the hydrolysis of ceramide to form sphingosine Cricetulus griseus ? - ? 89 3.5.1.23 additional information ceramidase catalyzes the hydrolysis of ceramide to form sphingosine Homo sapiens ? - ? 89 3.5.1.23 additional information ceramidase catalyzes the hydrolysis of ceramide to form sphingosine and free fatty acid Mus musculus ? - ? 89 3.5.1.23 additional information ceramidase catalyzes the hydrolysis of ceramide to generate sphingosine and fatty acid, also found to catalyze the reverse reaction Pseudomonas aeruginosa ? - ? 89 3.5.1.23 additional information ceramidase catalyzes the hydrolysis of ceramide to generate sphingosine and fatty acid, also found to catalyze the reverse reaction Rattus norvegicus ? - ? 89 3.5.1.23 additional information ceramidase deacylates ceramide to form sphingosine Rattus norvegicus ? - ? 89 3.5.1.23 additional information cleaves the N-acyl linkage of ceramides into a sphingosine base and a free fatty acid Mus musculus ? - ? 89 3.5.1.23 additional information ACER3 can catalyze the hydrolysis of unsaturated long-chain dihydroceramides as efficiently as unsaturated long-chain ceramides. ACER1 and ACER2, the homologues of ACER3, also catalyzed the hydrolysis of certain dihydroceramide species to generate dihydrosphingosine Homo sapiens ? - ? 89 3.5.1.23 additional information ceramidases are a group of enzymes that catalyze the hydrolysis of ceramides to generate sphingosine, which is phosphorylated to form sphingosine 1-phosphate Drosophila melanogaster ? - ? 89 3.5.1.23 additional information ACER2 shows a broader substrate specificity catalyzing the hydrolysis of various ceramides. The luminal ACER2 N-terminal tail, comprising 36 amino acid residues, is important for ACER2 activity, but the terminal 13 amino acid residues are not required for its activity Homo sapiens ? - ? 89 3.5.1.23 additional information ACER3 hydrolyzes C18:1-, C20:1-, C20:4-ceramides, C18:1-, C20:1-, C20:4-dihydroceramides, and C18:1-, C20:1-, C20:4-phytoceramides in vitro Homo sapiens ? - ? 89 3.5.1.23 additional information only the natural D-erythro-ceramide isomer is used as substrate of the four stereoisomers of ceramide. Dihydroceramide or phytoceramide forms, shortening the length of the alkyl backbone, methylation of the primary or secondary hydroxyl groups results in reduction or loss of the neutral ceramidase activity Homo sapiens ? - ? 89 3.5.1.23 additional information short acyl-chain ceramides (i.e. C16 acyl chain) are poor substrates for acid ceramidase Homo sapiens ? - ? 89 3.5.1.23 additional information long chain dihydroceramide dihydro-hexanoyl-D-erythro-sphingosine is resistant to the enzyme Rattus norvegicus ? - ? 89 3.5.1.23 additional information the enzyme prefers medium-chain or long chain ceramides over short-chain ceramides as substrates Tribolium castaneum ? - ? 89 3.5.1.23 additional information acid ceramidase catalyzes the hydrolysis of ceramide to constituent sphingoid base, sphingosine, and fatty acid Homo sapiens ? - ? 89 3.5.1.23 additional information the N-acyl moiety of ceramide is cleaved by ceramidases to form the lysolipid sphingosine that undergoes further phosphorylation to sphingosine-1-phosphate Homo sapiens ? - ? 89 3.5.1.23 additional information the N-acyl moiety of ceramide is cleaved by ceramidases to form the lysolipid sphingosine that undergoes further phosphorylation to sphingosine-1-phosphate Mus musculus ? - ? 89 3.5.1.23 additional information the Pseudomonas neutral CDase can hydrolyze ceramide in intact erythrocytes, leading to hemolysis. The reaction mechanism for ceramide hydrolysis in vivo appears to be the same as that for ceramide hydrolysis in vitro because the mutation of residues surrounding the catabolic zinc ion abolish the hemolytic activity along with ceramide hydrolysis by CDase in intact erythrocytes Pseudomonas aeruginosa ? - ? 89 3.5.1.23 additional information design and synthesis of a set of fluorescently labeled ceramides as substrates for acid and neutral ceramidases, overview. Although both the dyes, (acyl-(4-nitro-2,1,3-benzoxadiazol-7-yl)aminoethyl)-ceramide and acyl-Nile red-ceramide do not have any noticeable preference for the substitution at acyl or sphingosine part in ceramide towards hydrolysis by acid ceramidase, the ceramides with acyl-substituted NBD and Sph-substituted NR dyes have been found to be a better substrate for neutral ceramidase Homo sapiens ? - ? 89 3.5.1.23 additional information the enzyme can hydrolyze human skin-specific omega-hydroxyacyl ceramides Pseudomonas aeruginosa ? - ? 89 3.5.1.23 additional information the enzyme directly interacts with and binds steroidogenic factor 1, interaction site analysis, overview Homo sapiens ? - ? 89 3.5.1.23 additional information the enzyme has broad substrate specificity with preference for ceramides with a medium acyl-chain or a mono unsaturated long acyl-chain. The enzyme prefers ceramides with unsaturated acyl-chains over ceramides with saturated acyl-chains of the same length, and it does not use phytoceramides as substrates, suggesting that LsnCer is a ceramide-specific ceramidase Laodelphax striatellus ? - ? 89 3.5.1.23 additional information the enzyme is able to effectively degrade ceramide in the presence of Staphylococcus aureus-derived lipids or neutral detergents Pseudomonas aeruginosa ? - ? 89 3.5.1.23 additional information ADIPOR2 may have a preference for C18 ceramide substrate, but can also hydrolyse shorter (C6 ceramide) and longer (C24 ceramide) substrates, but to a lesser extent. Low overall ceramidase catalytic activity of ADIPOR2. Substrate binding structure, overview. The substrate amide carbonyl contacts the R278TM5 and Y328TM6 side chains, which are typical carbonyl-polarizing and oxyanion-stabilizing residues in zinc-dependent hydrolases Homo sapiens ? - ? 89 3.5.1.23 additional information recombinant NlnCDase shows a broad capacity to hydrolyze different ceramide varieties and has a preference for the short and medium chain ceramide, the substrate preference in descending order is C12-ceramide, C6-ceramide, and C16-ceramide. The enzyme shows low activity for the very-short chain C2-ceramide and the long chain substrates C20-ceramide and C24-ceramide Nilaparvata lugens ? - ? 89 3.5.1.23 additional information the enzyme has autoproteolytic activity Danio rerio ? - ? 89 3.5.1.23 additional information the enzyme performs autocatalysis. Substrate modeling suggests distinct catalytic mechanisms for substrate hydrolysis versus autocleavage Homo sapiens ? - ? 89 3.5.1.23 additional information ceramidase enhances phospholipase C-induced hemolysis by Pseudomonas aeruginosa Pseudomonas aeruginosa AN17 ? - ? 89 3.5.1.23 additional information ceramidase enhances cytotoxicity induced by hemolytic phospholipase C Pseudomonas aeruginosa AN17 ? - ? 89 3.5.1.23 additional information ceramidases are a group of enzymes that catalyze the hydrolysis of ceramides to generate sphingosine, which is phosphorylated to form sphingosine 1-phosphate Drosophila melanogaster BL-18012 ? - ? 89 3.5.1.23 myristic acid + sphingosine highest ceramide synthesis activity with myristic acid Rattus norvegicus N-myristoylsphingosine + H2O - ? 373858 3.5.1.23 N-((2S,3R)-1,3-dihydroxy-5-((2-oxo-2H-chromen-7-yl)oxy)pentan-2-yl)palmitamide + H2O - Homo sapiens palmitate + 2-amino-2,4-dideoxy-5-O-(2-oxo-2H-chromen-7-yl)-D-erythro-pentitol - ? 420990 3.5.1.23 N-acetylsphingosine + H2O - Homo sapiens acetic acid + sphingosine - ? 116240 3.5.1.23 N-acyl-sphingosine + H2O - Mus musculus a carboxylate + sphingosine - ? 383263 3.5.1.23 N-acyl-sphingosine + H2O the Asah2-encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the cellular levels of bioactive sphingolipid metabolites, ceramide, sphingosine, and sphingosine 1-phosphate, in the intestinal tract Mus musculus a carboxylate + sphingosine - ? 383263 3.5.1.23 N-acylsphingosine + H2O - Pseudomonas aeruginosa a carboxylate + sphingosine - ? 373913 3.5.1.23 N-acylsphingosine + H2O - Pseudomonas aeruginosa a carboxylate + sphingosine - r 373913 3.5.1.23 N-acylsphingosine + H2O AC catalyzes the hydrolysis of ceramide to sphingosine and fatty acid Homo sapiens a carboxylate + sphingosine - r 373913 3.5.1.23 N-acylsphingosine + H2O CDase catalyzes the hydrolysis of the N-acyl linkage of ceramide to produce sphingosine Rattus norvegicus a carboxylate + sphingosine - ? 373913 3.5.1.23 N-acylsphingosine + H2O CDase cleaves the N-acyl linkage of ceramide into sphingosine and free fatty acid, brain CDase is able to catalyze the reverse reaction, i.e. ceramide synthesis Rattus norvegicus a carboxylate + sphingosine - r 373913 3.5.1.23 N-acylsphingosine + H2O CDase cleaves the N-acyl linkage of ceramide to produce sphingosine and free fatty acid Rattus norvegicus a carboxylate + sphingosine - ? 373913 3.5.1.23 N-acylsphingosine + H2O CDase cleaves the N-acyl linkage of ceramide to produce sphingosine and free fatty acid Pseudomonas aeruginosa a carboxylate + sphingosine - ? 373913 3.5.1.23 N-acylsphingosine + H2O CDase hydrolyzes the amide bond in ceramides to yield free fatty acid and sphingosine Pseudomonas aeruginosa a carboxylate + sphingosine - ? 373913 3.5.1.23 N-acylsphingosine + H2O involved in the biosynthesis of ceramide, crucial enzyme for the regulation of the intracellular balance of the contents of ceramide, sphingosine and possibly sphingosine 1-phosphate Pseudomonas aeruginosa a carboxylate + sphingosine - ? 373913 3.5.1.23 N-acylsphingosine + H2O involved in the biosynthesis of ceramide, crucial enzyme for the regulation of the intracellular balance of the contents of ceramide, sphingosine and possibly sphingosine 1-phosphate Pseudomonas aeruginosa a carboxylate + sphingosine - r 373913 3.5.1.23 N-acylsphingosine + H2O the carboxylate is a fatty acid Homo sapiens carboxylate + sphingosine - ? 432866 3.5.1.23 N-acylsphingosine + H2O the carboxylate is a fatty acid Mus musculus carboxylate + sphingosine - ? 432866 3.5.1.23 N-acylsphingosine + H2O the carboxylate is a fatty acid Rattus norvegicus carboxylate + sphingosine - ? 432866 3.5.1.23 N-acylsphingosine + H2O the carboxylate is a fatty acid Saccharomyces cerevisiae carboxylate + sphingosine - r 432866 3.5.1.23 N-acylsphingosine + H2O the carboxylate is a fatty acid, Ypc1p can catalyse the reverse reaction, i.e. the condensation of non-esterified fatty acids with phytosphingosine or dihydrosphingosine. The cysteine residues at positions 27 and 219 are required for reverse ceramidase activity Saccharomyces cerevisiae carboxylate + sphingosine - r 432866 3.5.1.23 N-acylsphingosine + H2O the carboxylate is a fatty acid. The cysteine residues at positions 27 and 219 are required for reverse ceramidase activity Saccharomyces cerevisiae carboxylate + sphingosine - r 432866 3.5.1.23 N-arachidonoyl-ceramide + H2O - Homo sapiens sphingosine + arachidonate - ? 414929 3.5.1.23 N-arachidonoyl-dihydroceramide + H2O - Homo sapiens dihydrosphingosine + arachidonate - ? 414930 3.5.1.23 N-arachidonoyl-phytoceramide + H2O - Homo sapiens phytosphingosine + arachidonate - ? 414931 3.5.1.23 N-arachidoyl-D-erythro-sphingosine + H2O low activity Laodelphax striatellus arachidonate + D-erythro-sphingosine - ? 433985 3.5.1.23 N-caproylsphingosine + H2O about 25% activity compared to N-oleoylsphingosine Tribolium castaneum caproate + sphingosine - ? 421047 3.5.1.23 N-dodecanoyl-7-nitrobenz-2-oxa-1,3,4-diazole-D-erythro-dihydrosphingosine + H2O - Mycobacterium tuberculosis 12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodecanoate + D-erythro-dihydrosphingosine - r 414947 3.5.1.23 N-dodecanoyl-7-nitrobenz-2-oxa-1,3,4-diazole-D-erythro-phytosphingosine + H2O - Mycobacterium tuberculosis 12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodecanate + D-erythro-phytosphingosine - r 414948 3.5.1.23 N-dodecanoyl-7-nitrobenz-2-oxa-1,3,4-diazole-D-erythro-sphingosine + H2O i.e. C12-NBD-Cer Mycobacterium tuberculosis 12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodecanate + D-erythro-sphingosine - r 414949 3.5.1.23 N-hexanoyl-D-erythro-sphingosine + H2O 100.3% hydrolysis compared to N-palmitoyl-D-erythro-sphingosine Rattus norvegicus sphingosine + hexanoate - ? 421086 3.5.1.23 N-hexanoyl-D-erythro-sphingosine + H2O - Camelus dromedarius hexanoate + D-erythro-sphingosine - ? 434010 3.5.1.23 N-hexanoyl-D-erythro-sphingosine + H2O - Laodelphax striatellus hexanoate + D-erythro-sphingosine - ? 434010 3.5.1.23 N-hexanoyl-sphingosine + H2O - Homo sapiens hexanoate + sphingosine - ? 434011 3.5.1.23 N-hexanoylsphingosine + H2O - Homo sapiens hexanoic acid + sphingosine - ? 116241 3.5.1.23 N-lauroyl-D-erythro-sphingosine + H2O most efficient substrate, 293.2% hydrolysis compared to N-palmitoyl-D-erythro-sphingosine Rattus norvegicus sphingosine + dodecanoate - r 421089 3.5.1.23 N-lauroyl-D-erythro-sphingosine + H2O best substrate Laodelphax striatellus laurate + D-erythro-sphingosine - ? 434014 3.5.1.23 N-lauroyl-D-erythro-sphingosine + H2O the enzyme catalyzes also the reverse reaction of ceramide synthesis Camelus dromedarius laurate + D-erythro-sphingosine - r 434014 3.5.1.23 N-lauroylceramide + H2O - Rattus norvegicus laureate + sphingosine - ? 434015 3.5.1.23 N-lauroylsphingosine + H2O - Homo sapiens laurate + sphingosine - ? 373931 3.5.1.23 N-lauroylsphingosine + H2O more efficient hydrolysis than of N-palmitoylsphingosine and N-stearoylsphingosine, less efficient hydrolysis than of 4-nitrobenzo-2-oxa-1,3-diazoyl-N-dodecanoylsphingosine Rattus norvegicus laurate + sphingosine - ? 373931 3.5.1.23 N-lauroylsphingosine + H2O about 95% activity compared to N-oleoylsphingosine Tribolium castaneum laurate + sphingosine - ? 373931 3.5.1.23 N-lauroylsphingosine + H2O - Homo sapiens lauric acid + sphingosine - ? 373932 3.5.1.23 N-lauroylsphingosine + H2O optimal substrate, AC also efficiently catalyzes ceramide synthesis Homo sapiens lauric acid + sphingosine - r 373932 3.5.1.23 N-laurylsphingosine + H2O - Mus musculus laureate + sphingosine - ? 385313 3.5.1.23 N-lignoceroyl-D-erythro-sphingosine + H2O 25.1% hydrolysis compared to N-palmitoyl-D-erythro-sphingosine Rattus norvegicus sphingosine + tetracosanoate - ? 421090 3.5.1.23 N-lignoceroyl-D-erythro-sphingosine + H2O low activity Camelus dromedarius lignocerate + D-erythro-sphingosine - ? 434016 3.5.1.23 N-lignoceroyl-D-erythro-sphingosine + H2O low activity Laodelphax striatellus lignocerate + D-erythro-sphingosine - ? 434016 3.5.1.23 N-myristoyl-D-erythro-sphingosine + H2O 212.2% hydrolysis compared to N-palmitoyl-D-erythro-sphingosine Rattus norvegicus sphingosine + myristate - ? 421096 3.5.1.23 N-myristoyl-D-erythro-sphingosine + H2O best substrate Camelus dromedarius myristate + D-erythro-sphingosine - ? 434017 3.5.1.23 N-myristoyl-D-sphingosine + H2O - Pseudomonas sp. ? - ? 401471 3.5.1.23 N-nervonoyl-D-erythro-sphingosine + H2O - Laodelphax striatellus nervonoate + D-erythro-sphingosine - ? 434018 3.5.1.23 N-nervonoylsphingosine + H2O about 80% activity compared to N-oleoylsphingosine Tribolium castaneum nervonate + sphingosine - ? 421098 3.5.1.23 N-octanoylsphinganine + H2O - Homo sapiens octanoic acid + sphingosine - ? 116244 3.5.1.23 N-octanoylsphingosine + H2O - Homo sapiens octanoic acid + sphingosine - ? 116242 3.5.1.23 N-octanoylsphingosine + H2O - Homo sapiens octanoic acid + sphingosine - r 116242 3.5.1.23 N-oleoyl-ceramide + H2O - Homo sapiens sphingosine + oleate - ? 414985 3.5.1.23 N-oleoyl-D-erythro-ceramide + H2O common substrate in erythrocytes Mus musculus oleate + D-erythro-sphingosine - ? 413482 3.5.1.23 N-oleoyl-D-erythro-ceramide + H2O common substrate in erythrocytes Homo sapiens oleate + D-erythro-sphingosine - ? 413482 3.5.1.23 N-oleoyl-D-erythro-ceramide + H2O i.e. D-e-C18:1-ceramide, common substrate in erythrocytes Mus musculus oleate + D-erythro-sphingosine - ? 413482 3.5.1.23 N-oleoyl-D-erythro-ceramide + H2O i.e. D-e-C18:1-ceramide, common substrate in erythrocytes Homo sapiens oleate + D-erythro-sphingosine - ? 413482 3.5.1.23 N-oleoyl-D-erythro-sphingosine + H2O - Laodelphax striatellus oleate + D-erythro-sphingosine - ? 434023 3.5.1.23 N-oleoyl-D-sphingosine + H2O - Homo sapiens oleate + sphingosine - ? 450369 3.5.1.23 N-oleoyl-dihydroceramide + H2O - Homo sapiens dihydrosphingosine + oleate - ? 414986 3.5.1.23 N-oleoyl-phytoceramide + H2O - Homo sapiens phytosphingosine + oleate - ? 414987 3.5.1.23 N-oleoylsphingosine + H2O - Cavia porcellus oleic acid + sphingosine - ? 116243 3.5.1.23 N-oleoylsphingosine + H2O - Homo sapiens oleic acid + sphingosine - ? 116243 3.5.1.23 N-oleoylsphingosine + H2O - Homo sapiens oleic acid + sphingosine - r 116243 3.5.1.23 N-oleoylsphingosine + H2O 100% activity Tribolium castaneum oleate + sphingosine - ? 421106 3.5.1.23 N-oleyldihydro-sphingosine + H2O - Rattus norvegicus ? - r 116227 3.5.1.23 N-palmitoyl sphingosine + H2O - Pseudomonas aeruginosa palmitic acid + sphingosine - ? 373942 3.5.1.23 N-palmitoyl-D-erythro-sphingosine + H2O - Mus musculus D-erythro-sphingosine + palmitic acid - ? 406031 3.5.1.23 N-palmitoyl-D-erythro-sphingosine + H2O 100% hydrolysis Rattus norvegicus sphingosine + palmitate - r 421107 3.5.1.23 N-palmitoyl-D-erythro-sphingosine + H2O - Camelus dromedarius palmitate + D-erythro-sphingosine - ? 434024 3.5.1.23 N-palmitoyl-D-erythro-sphingosine + H2O - Laodelphax striatellus palmitate + D-erythro-sphingosine - ? 434024 3.5.1.23 N-palmitoyldihydrosphingosine + H2O - Rattus norvegicus ? - r 116229 3.5.1.23 N-palmitoylphytosphingosine + H2O - Mus musculus ? - ? 116235 3.5.1.23 N-palmitoylphytosphingosine + H2O - Pseudomonas aeruginosa ? - r 116235 3.5.1.23 N-palmitoylsphinganine + H2O - Mus musculus palmitic acid + sphinganine - ? 116233 3.5.1.23 N-palmitoylsphinganine + H2O - Homo sapiens palmitic acid + sphinganine - ? 116233 3.5.1.23 N-palmitoylsphinganine + H2O - Pseudomonas aeruginosa palmitic acid + sphinganine - r 116233 3.5.1.23 N-palmitoylsphinganine + H2O low hydrolysis rate Rattus norvegicus palmitate + sphinganine - ? 373943 3.5.1.23 N-palmitoylsphingosine + H2O - Cavia porcellus palmitic acid + sphingosine - ? 116228 3.5.1.23 N-palmitoylsphingosine + H2O - Mus musculus palmitic acid + sphingosine - ? 116228 3.5.1.23 N-palmitoylsphingosine + H2O - Homo sapiens palmitic acid + sphingosine - r 116228 3.5.1.23 N-palmitoylsphingosine + H2O - Rattus norvegicus palmitic acid + sphingosine - r 116228 3.5.1.23 N-palmitoylsphingosine + H2O - Pseudomonas aeruginosa palmitic acid + sphingosine - r 116228 3.5.1.23 N-palmitoylsphingosine + H2O less efficient hydrolysis than of N-lauroylsphingosine, more efficient hydrolysis than of N-stearoylsphingosine Rattus norvegicus palmitate + sphingosine - ? 373944 3.5.1.23 N-palmitoylsphingosine + H2O about 65% activity compared to N-oleoylsphingosine Tribolium castaneum palmitate + sphingosine - ? 373944 3.5.1.23 N-stearoyl-D-erythro-sphingosine + H2O 98.5% hydrolysis compared to N-palmitoyl-D-erythro-sphingosine Rattus norvegicus sphingosine + stearate - ? 421109 3.5.1.23 N-stearoyl-D-erythro-sphingosine + H2O - Camelus dromedarius stearate + D-erythro-sphingosine - ? 434025 3.5.1.23 N-stearoyl-D-erythro-sphingosine + H2O low activity Laodelphax striatellus stearate + D-erythro-sphingosine - ? 434025 3.5.1.23 N-stearoylphytosphingosine + H2O - Mus musculus ? - ? 116236 3.5.1.23 N-stearoylphytosphingosine + H2O - Pseudomonas aeruginosa ? - r 116236 3.5.1.23 N-stearoylsphinganine + H2O - Mus musculus stearic acid + sphinganine - ? 116234 3.5.1.23 N-stearoylsphinganine + H2O - Homo sapiens stearic acid + sphinganine - ? 116234 3.5.1.23 N-stearoylsphinganine + H2O - Pseudomonas aeruginosa stearic acid + sphinganine - r 116234 3.5.1.23 N-stearoylsphinganine + H2O low hydrolysis rate Rattus norvegicus stearate + sphinganine - ? 373950 3.5.1.23 N-stearoylsphingosine + H2O - Mus musculus stearic acid + sphingosine - ? 116230 3.5.1.23 N-stearoylsphingosine + H2O - Rattus norvegicus stearic acid + sphingosine - r 116230 3.5.1.23 N-stearoylsphingosine + H2O - Pseudomonas aeruginosa stearic acid + sphingosine - r 116230 3.5.1.23 N-stearoylsphingosine + H2O less efficient hydrolysis than of N-lauroylsphingosine and N-palmitoylsphingosine Rattus norvegicus stearate + sphingosine - ? 373951 3.5.1.23 N-[(2S,3R,4E)-1,3-dihydroxy-14-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]tetradec-4-en-2-yl]hexadecanamide + H2O 5.6% hydrolysis Homo sapiens ? - ir 434029 3.5.1.23 N-[(2S,3R,4E)-1,3-dihydroxy-14-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]tetradec-4-en-2-yl]hexadecanamide + H2O 6.2% hydrolysis Homo sapiens ? - ir 434029 3.5.1.23 N-[(2S,3R,4E)-1,3-dihydroxynonadec-4-en-2-yl]-12-[[9-(ethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]dodecanamide + H2O 6.1% hydrolysis Homo sapiens ? - ir 434030 3.5.1.23 N-[(2S,3R,4E)-1,3-dihydroxynonadec-4-en-2-yl]-12-[[9-(ethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]dodecanamide + H2O 63.3% hydrolysis Homo sapiens ? - ir 434030 3.5.1.23 N-[(2S,3R,4E)-13-[[9-(ethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]-1,3-dihydroxytridec-4-en-2-yl]hexadecanamide + H2O 3.9% hydrolysis Homo sapiens ? - ir 434031 3.5.1.23 N-[(2S,3R,4E)-13-[[9-(ethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]-1,3-dihydroxytridec-4-en-2-yl]hexadecanamide + H2O 6.3% hydrolysis Homo sapiens ? - ir 434031 3.5.1.23 N-[(2S,3R,4E)-7-[[9-(diethylamino)-5-oxo-5H-benzo[a]phenoxazin-3-yl]oxy]-1,3-dihydroxyhept-4-en-2-yl]-11-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]undecanamide + H2O fluorescently labelled ceramide analogue, 4.0% hydrolęsis Homo sapiens ? - ir 434032 3.5.1.23 N-[(2S,3R,4E)-7-[[9-(diethylamino)-5-oxo-5H-benzo[a]phenoxazin-3-yl]oxy]-1,3-dihydroxyhept-4-en-2-yl]-11-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]undecanamide + H2O fluorescently labelled ceramide analogue, 5.8% hydrolęsis Mus musculus ? - ir 434032 3.5.1.23 N-[12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanoyl]D-erythro-sphingosine + H2O substrate C12-NBD ceramide Homo sapiens N-[12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanoate] + D-erythro-sphingosine - ? 450380 3.5.1.23 N-[12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]lauroyl]-phytosphingosine + H2O i.e. D-ribo-C12-NBD-phytoceramide Homo sapiens N-12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]laurate + phytosphingosine - ? 414999 3.5.1.23 N-[12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]lauroyl]-sphingosine + H2O i.e. C12-NBD-ceramide Aspergillus oryzae RIB40 N-12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]laurate + sphingosine - r 415000 3.5.1.23 NBD-C12-ceramide + H2O - Homo sapiens ? - ? 450404 3.5.1.23 octadecanoyl-ceramide + H2O i.e. C18-ceramide Aspergillus oryzae RIB40 octadecanoate + sphingosine - r 415066 3.5.1.23 octanoyl-D-erythro-sphingosine + H2O substrate activity assay Mus musculus ? - ? 406238 3.5.1.23 octanoyl-sphingosine + H2O - Rattus norvegicus octanoate + sphingosine - ? 385471 3.5.1.23 palmitoyl sphingosine + H2O substrate activity assay Oryza sativa palmitate + sphingosine - ? 406323 3.5.1.23 palmitoyl sphingosine + H2O substrate activity assay Drosophila melanogaster palmitate + sphingosine - ? 406323 3.5.1.23 palmitoyl-D-[erythro-9,10]sphingosine + H2O - Mus musculus palmitate + D-erythro-sphingosine - ? 385502 3.5.1.23 palmitoyl-sphingosine + H2O - Rattus norvegicus palmitate + sphingosine - ? 385503 3.5.1.23 palmitoyl-sphingosine + H2O - Rattus norvegicus palmitate + sphingosine - r 385503 3.5.1.23 palmitoyl-sphingosine + H2O - Homo sapiens palmitate + sphingosine - r 385503 3.5.1.23 phytoceramide + H2O - Homo sapiens phytosphingosine + fatty acid - ? 413533 3.5.1.23 sphingosine + fatty acid - Rattus norvegicus ceramide - r 116226 3.5.1.23 sphingosine + myristic acid + H2O at pH 8.0 Pseudomonas sp. C14-ceramide - ? 401905