3.5.1.12 (N-D,L-lipoyl-p-aminobenzoic acid) + H2O - Homo sapiens ? - ? 116060 3.5.1.12 (N-D,L-lipoyl-p-aminobenzoic acid) + H2O - Rattus rattus ? - ? 116060 3.5.1.12 1'-N-methoxycarbonyl-biocytin + H2O - Sus scrofa 1-N-methoxycarbonyl-biotin + L-lysine - ? 116046 3.5.1.12 2-(N-biotinyl)-amino-5-nitro-benzoic acid + H2O - Sus scrofa biotin + 2-amino-5-nitrobenzoic acid - ? 116043 3.5.1.12 2-(N-biotinyl)-amino-5-nitro-benzoic acid + H2O - Lacticaseibacillus casei biotin + 2-amino-5-nitrobenzoic acid - ? 116043 3.5.1.12 3-(N-biotinyl)-amino-6-nitrobenzoic acid + H2O - Sus scrofa biotin + 3-amino-6-nitrobenzoic acid - ? 116044 3.5.1.12 3-(N-biotinyl)-amino-6-nitrobenzoic acid + H2O - Lacticaseibacillus casei biotin + 3-amino-6-nitrobenzoic acid - ? 116044 3.5.1.12 biocytin + ? - Homo sapiens biotin + L-lysine - ? 404528 3.5.1.12 biocytin + H2O - Homo sapiens biotin + L-lysine - ? 370882 3.5.1.12 biocytin + H2O natural substrate Homo sapiens biotin + L-lysine - ? 370882 3.5.1.12 biocytin + H2O natural substrate, biotinidase is responsible for cleaving biocytin, thereby liberating and recycling biotin Homo sapiens biotin + L-lysine - ? 370882 3.5.1.12 biocytin + H2O biotinylation of histones by biotinidase depends on the hydrolytic cleavage of biocytin (biotinyl-epsilon-lysine), coupled to the transfer of biotinyl residue to free amino groups in histones. K4, K9 and K18 in histone H3 are good targets for biotinylation, K14 and K23 are relatively poor targets Homo sapiens biotin + L-lysine - ? 370882 3.5.1.12 biocytin + H2O i.e. biotin-epsilon-lysine. The enzyme is involved in modification of histones by covalent attachment of the vitamin biotin. A reaction mechanism is proposed by which cleavage of biocytin by biotinidase leads to the formation of a biotinyl-thioester intermediate (cysteine-bound biotin) at or near the active site of biotinidase. In the next step, the biotinyl moiety is transferred from the thioester to the epsilon-amino group of lysine in histones. Biotinidase may catalyze both biotinylation and debiotinylation of histones Homo sapiens biotin + L-lysine - ? 370882 3.5.1.12 biocytin + H2O i.e. biotin-epsilon-lysine Homo sapiens biotin + L-lysine - ? 370882 3.5.1.12 biocytin + H2O i.e. biotin-epsilon-lysine. Because polylysine is readily biotinylated by biotinidase in the presence of biocytin, whereas polyarginine is not biotinylated, the enzyme likely transfers biotin to the epsilon-amino group of lysyl residues Homo sapiens biotin + L-lysine - ? 370882 3.5.1.12 biocytin + H2O the enzyme belongs to the nitrilase superfamily Homo sapiens biotin + L-lysine - ? 370882 3.5.1.12 biotin amide + H2O - Cavia porcellus ? - ? 369814 3.5.1.12 biotin amide + H2O - Homo sapiens ? - ? 369814 3.5.1.12 biotin amide + H2O - Sus scrofa ? - ? 369814 3.5.1.12 biotin amide + H2O - Oryctolagus cuniculus ? - ? 369814 3.5.1.12 biotin amide + H2O - Enterococcus faecalis ? - ? 369814 3.5.1.12 biotin amide + H2O - Lacticaseibacillus casei ? - ? 369814 3.5.1.12 biotin amide + H2O - Rattus rattus ? - ? 369814 3.5.1.12 biotin amide + H2O - Gallus sp. ? - ? 369814 3.5.1.12 biotin amide + H2O very short biotinyl peptides, enzyme is not active when biotin is attached to large native proteins, for example intact holocarboxylases Homo sapiens ? - ? 369814 3.5.1.12 biotin amide + H2O cleaves D-biotinylamides and esters, recycling of the vitamin Homo sapiens ? - ? 369814 3.5.1.12 biotin amide + H2O cleaves D-biotinylamides and esters, recycling of the vitamin Sus scrofa ? - ? 369814 3.5.1.12 biotin amide + H2O soluble bound biotin Sus scrofa ? - ? 369814 3.5.1.12 biotin amide + H2O good substrate, Cys-245 is likely the active site cysteine, formation of a thioester intermediate between biotin and cysteine Homo sapiens biotin + NH3 - ? 372577 3.5.1.12 biotin methyl ester + H2O - Sus scrofa ? - ? 116047 3.5.1.12 biotin methyl ester + H2O - Enterococcus faecalis ? - ? 116047 3.5.1.12 biotin methyl ester + H2O - Lacticaseibacillus casei ? - ? 116047 3.5.1.12 biotin methyl ester + H2O - Gallus sp. ? - ? 116047 3.5.1.12 biotinyl-4-amino benzoic acid + H2O - Homo sapiens biotin + 4-aminobenzoic acid - ? 449683 3.5.1.12 biotinyl-4-aminobenzoic acid + H2O - Homo sapiens biotin + 4-aminobenzoic acid - ? 116050 3.5.1.12 biotinyl-4-aminobenzoic acid + H2O - Sus scrofa biotin + 4-aminobenzoic acid - ? 116050 3.5.1.12 biotinyl-4-aminobenzoic acid + H2O colorimetric synthetic substrate Homo sapiens biotin + 4-aminobenzoic acid - ? 116050 3.5.1.12 biotinyl-6-aminoquinoline + H2O - Mus musculus biotin + 6-aminoquinoline - ? 116051 3.5.1.12 biotinyl-6-aminoquinoline + H2O - Homo sapiens biotin + 6-aminoquinoline - ? 116051 3.5.1.12 biotinyl-6-aminoquinoline + H2O - Rattus norvegicus biotin + 6-aminoquinoline - ? 116051 3.5.1.12 biotinyl-6-aminoquinoline + H2O - Lacticaseibacillus casei biotin + 6-aminoquinoline - ? 116051 3.5.1.12 biotinyl-6-aminoquinoline + H2O BAQ, fluorogenic substrate as assay for biotinidase Homo sapiens biotin + 6-aminoquinoline - ? 116051 3.5.1.12 biotinyl-6-aminoquinoline + H2O - Lacticaseibacillus casei Shirota biotin + 6-aminoquinoline - ? 116051 3.5.1.12 biotinyl-di-iodotyramine + H2O - Homo sapiens biotin + di-iodotyramine - ? 116063 3.5.1.12 biotinyl-di-iodotyramine + H2O radioactive assay for early diagnosis of biotinidase defiency in serum Homo sapiens biotin + di-iodotyramine - ? 116063 3.5.1.12 biotinyl-L-aspartate + H2O - Sus scrofa biotin + L-aspartate - ? 116049 3.5.1.12 biotinyl-L-aspartate + H2O - Enterococcus faecalis biotin + L-aspartate - ? 116049 3.5.1.12 biotinyl-L-aspartate + H2O - Lacticaseibacillus casei biotin + L-aspartate - ? 116049 3.5.1.12 biotinyl-L-aspartate + H2O - Gallus sp. biotin + L-aspartate - ? 116049 3.5.1.12 biotinyl-monoiodotyramine + H2O radioactive assay for early diagnosis of biotinidase defiency in serum Homo sapiens biotin + monoiodotyramine - ? 116062 3.5.1.12 biotinyl-p-aminobenzoate + H2O - Homo sapiens ? - ? 396613 3.5.1.12 dynorphin A (1-6) + H2O - Homo sapiens ? - ? 116054 3.5.1.12 dynorphin A (1-7) + H2O - Homo sapiens ? - ? 116059 3.5.1.12 epsilon-N-(D,L-lipoyl)-L-lysine + H2O - Homo sapiens ? - ? 116061 3.5.1.12 epsilon-N-(D,L-lipoyl)-L-lysine + H2O - Rattus rattus ? - ? 116061 3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O - Cavia porcellus biotin + L-lysine - ir 116041 3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O - Homo sapiens biotin + L-lysine - ir 116041 3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O - Sus scrofa biotin + L-lysine - ir 116041 3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O - Oryctolagus cuniculus biotin + L-lysine - ir 116041 3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O - Enterococcus faecalis biotin + L-lysine - ir 116041 3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O - Lacticaseibacillus casei biotin + L-lysine - ir 116041 3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O - Rattus rattus biotin + L-lysine - ir 116041 3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O - Gallus sp. biotin + L-lysine - ir 116041 3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O cannot cleave biocytin that is bound to avidin Homo sapiens biotin + L-lysine - ir 116041 3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O biocytin, soluble bound biotin Sus scrofa biotin + L-lysine - ir 116041 3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O - Cavia porcellus ? - ? 369813 3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O - Homo sapiens ? - ? 369813 3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O - Sus scrofa ? - ? 369813 3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O - Enterococcus faecalis ? - ? 369813 3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O - Lacticaseibacillus casei ? - ? 369813 3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O - Rattus rattus ? - ? 369813 3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O - Gallus sp. ? - ? 369813 3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O biocytin, soluble bound biotin Sus scrofa ? - ? 369813 3.5.1.12 Leu-enkephalin + H2O - Homo sapiens ? - ? 116056 3.5.1.12 Leu-enkephalin amide + H2O - Homo sapiens ? - ? 116058 3.5.1.12 lipoyl-4-aminobenzoic acid + H2O - Rattus rattus ? - ? 453322 3.5.1.12 lipoyllysine + H2O - Rattus rattus ? - ? 453323 3.5.1.12 Met-enkephalin + H2O - Homo sapiens ? - ? 116055 3.5.1.12 Met-enkephalin + H2O suitable natural substrate for biotinidase Homo sapiens ? - ? 116055 3.5.1.12 Met-enkephalin amide + H2O - Homo sapiens ? - ? 116057 3.5.1.12 additional information no hydrolysis of acetamide or p-nitroacetanilide Homo sapiens ? - ? 89 3.5.1.12 additional information biotinidase is essential for recycling the vitamin biotin and for transferring biotin to proteins Homo sapiens ? - ? 89 3.5.1.12 additional information enzymatic activity including biotinidase is not solely responsible for the biotin release from biotinylated IgGs. It is speculated that heat-stable, low-molecular-weight chemical factors present in plasma also catalyze hydrolysis of the amide bond to the carboxyl group of biotin Homo sapiens ? - ? 89 3.5.1.12 additional information biotinylamidopropyl-N,N-(dipicolyl)amine and N,N-(dipicolyl)biotinamine are resistant to biotinidase activity Mus musculus ? - ? 89 3.5.1.12 additional information lysine residues K9 and K13 in N-terminus of human histones H2A and H2AX are targets for biotinylation. K125, K127 and K129 in the C-terminus of histone H2A are targets for biotinylation Homo sapiens ? - ? 89 3.5.1.12 additional information biotinidase, an amidohydrolase, catalyzes the cleavage of biotin from biocytin or biotinylated peptides formed from proteolytic degradation of the holocarboxylases and other biotinylated proteins Homo sapiens ? - ? 89 3.5.1.12 N,N-(dipicolyl)biotinamido-Boc-lysine + H2O - Mus musculus N,N-(dipicolyl)biotinamine + Boc-L-lysine - ? 385204 3.5.1.12 N-(+)biotinyl-4-aminobenzoic acid + H2O - Homo sapiens 4-aminobenzoic acid + biotin - ? 397834 3.5.1.12 N-(1'-N-methoxycarbonyl-biotinyl)p-aminobenzoic acid + H2O - Sus scrofa 1-N-methoxycarbonyl-biotin + 4-aminobenzoic acid - ? 116045 3.5.1.12 N-biotinyl-3-amino benzoate + H2O - Homo sapiens biotin + 3-aminobenzoate - ? 414939 3.5.1.12 N-biotinyl-3-aminobenzoic acid + H2O - Homo sapiens biotin + 3-aminobenzoic acid - ? 116053 3.5.1.12 N-biotinyl-3-aminobenzoic acid + H2O - Sus scrofa biotin + 3-aminobenzoic acid - ? 116053 3.5.1.12 N-biotinyl-beta-alanine + H2O - Sus scrofa biotin + beta-alanine - ? 116048 3.5.1.12 N-biotinyl-beta-alanine + H2O - Enterococcus faecalis biotin + beta-alanine - ? 116048 3.5.1.12 N-biotinyl-beta-alanine + H2O - Lacticaseibacillus casei biotin + beta-alanine - ? 116048 3.5.1.12 N-biotinyl-beta-alanine + H2O - Gallus sp. biotin + beta-alanine - ? 116048 3.5.1.12 N-biotinyl-p-aminobenzoate + ? - Rattus norvegicus p-aminobenzoate + biotin - ? 405984 3.5.1.12 N-DL-dethiobiotinyl-p-aminobenzoic acid + H2O - Homo sapiens dethiobiotin + 4-aminobenzoic acid - ? 116052 3.5.1.12 p-(N-biotinylamino)benzoic acid + H2O - Homo sapiens biotin + p-aminobenzoic acid - ir 116042 3.5.1.12 p-(N-biotinylamino)benzoic acid + H2O - Sus scrofa biotin + p-aminobenzoic acid - ir 116042 3.5.1.12 p-(N-biotinylamino)benzoic acid + H2O - Enterococcus faecalis biotin + p-aminobenzoic acid - ir 116042 3.5.1.12 p-(N-biotinylamino)benzoic acid + H2O - Lacticaseibacillus casei biotin + p-aminobenzoic acid - ir 116042 3.5.1.12 p-(N-biotinylamino)benzoic acid + H2O - Rattus rattus biotin + p-aminobenzoic acid - ? 116042 3.5.1.12 p-(N-biotinylamino)benzoic acid + H2O - Gallus sp. biotin + p-aminobenzoic acid - ir 116042 3.5.1.12 p-(N-biotinylamino)benzoic acid + H2O PAB, artificial substrate Homo sapiens biotin + p-aminobenzoic acid - ir 116042