3.4.24.11 (7-methoxycoumarin-4-yl)acetyl-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(2,4-dinitrophenyl) + H2O a bradykinin-based peptide substrate QFS Homo sapiens ? - ? 449098 3.4.24.11 (7-methoxycoumarin-4-yl)acetyl-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys-(2,4-dinitrophenyl) + H2O bradykinin-based quenched fluorescent substrate assay Homo sapiens (7-methoxycoumarin-4-yl)acetyl-Arg-Pro-Pro-Gly-Phe-Ser-Ala + Phe-Lys-(2,4-dinitrophenyl) - ? 433382 3.4.24.11 2 KFRRQRPRLSHKGPMPF + 2 H2O - Homo sapiens KFRRQRPR + LSHKGPMPF + KFRRQRPRL + SHKGPMPF - ir 449176 3.4.24.11 2 LVQPRGSRNGPGPWQGGRRKFRRQRPRLSHKGPMPF + 2 H2O - Homo sapiens LVQPRGSRNGPGPWQGGRRKFRRQRPRL + SHKGPMPF + LVQPRGSRNGPGPWQGGRRKFRRQRPR + LSHKGPMPF - ir 449177 3.4.24.11 2 pGlu-RPRLSHKGPMPF + 2 H2O - Homo sapiens pGlu-RPRL + pGlu-RPR + SHKGPMPF + LSHKGPMPF - ir 449178 3.4.24.11 2-aminobenzoyl-ARFK-2,4-dinitrophenyl ester + H2O - Homo sapiens 2-aminobenzoyl-AR + FK-2,4-dinitrophenyl ester - ir 395434 3.4.24.11 2-aminobenzoyl-DRFK-2,4-dinitrophenyl ester + H2O - Homo sapiens 2-aminobenzoyl-DR + FK-2,4-dinitrophenyl ester - ir 395435 3.4.24.11 2-aminobenzoyl-FRFK-2,4-dinitrophenyl ester + H2O - Homo sapiens 2-aminobenzoyl-FR + FK-2,4-dinitrophenyl ester - ir 395438 3.4.24.11 2-aminobenzoyl-HRFK-2,4-dinitrophenyl ester + H2O - Homo sapiens 2-aminobenzoyl-HR + FK-2,4-dinitrophenyl ester - ir 395440 3.4.24.11 2-aminobenzoyl-IRFK-2,4-dinitrophenyl ester + H2O - Homo sapiens 2-aminobenzoyl-IR + FK-2,4-dinitrophenyl ester - ir 395441 3.4.24.11 2-aminobenzoyl-KRFK-2,4-dinitrophenyl ester + H2O - Homo sapiens 2-aminobenzoyl-KR + FK-2,4-dinitrophenyl ester - ir 395443 3.4.24.11 2-aminobenzoyl-LRFK-2,4-dinitrophenyl ester + H2O - Homo sapiens 2-aminobenzoyl-LR + FK-2,4-dinitrophenyl ester - ir 395444 3.4.24.11 2-aminobenzoyl-NRFK-2,4-dinitrophenyl ester + H2O - Homo sapiens 2-aminobenzoyl-NR + FK-2,4-dinitrophenyl ester - ir 395445 3.4.24.11 2-aminobenzoyl-RAFK-2,4-dinitrophenyl ester + H2O - Homo sapiens 2-aminobenzoyl-RA + FK-2,4-dinitrophenyl ester - ir 395446 3.4.24.11 2-aminobenzoyl-RDFK-2,4-dinitrophenyl ester + H2O - Homo sapiens 2-aminobenzoyl-RD + FK-2,4-dinitrophenyl ester - ir 395447 3.4.24.11 2-aminobenzoyl-REFK-2,4-dinitrophenyl ester + H2O - Homo sapiens 2-aminobenzoyl-RE + FK-2,4-dinitrophenyl ester - ir 395448 3.4.24.11 2-aminobenzoyl-rGF-N-(2,4-dinitrophenyl)ethylenediamine + H2O - Homo sapiens 2-aminobenzoyl-D-Arg-Gly + Phe-N-(2,4-dinitrophenyl)ethylenediamine - ir 395449 3.4.24.11 2-aminobenzoyl-RGFK(Dnp)-OH + H2O - Homo sapiens 2-aminobenzoyl-RG + FK-2,4-dinitrophenyl ester - ir 395450 3.4.24.11 2-aminobenzoyl-RGFK-2,4-dinitrophenyl amide + H2O - Homo sapiens 2-aminobenzoyl-RG + FK-2,4-dinitrophenyl amide - ir 395451 3.4.24.11 2-aminobenzoyl-RGFK-2,4-dinitrophenyl ester + H2O - Homo sapiens 2-aminobenzoyl-RG + FK-2,4-dinitrophenyl ester - ir 395452 3.4.24.11 2-aminobenzoyl-rGL-N-(2,4-dinitrophenyl)ethylenediamine + H2O - Homo sapiens 2-aminobenzoyl-D-Arg-Gly + Leu-N-(2,4dinitrophenyl)ethylenediamine - ir 395453 3.4.24.11 2-aminobenzoyl-rGV-N-(2,4-dinitrophenyl)ethylenediamine + H2O - Homo sapiens 2-aminobenzoyl-D-Arg-Gly + Val-N-(2,4-dinitrophenyl)ethylenediamine - ir 395454 3.4.24.11 2-aminobenzoyl-RHFK-2,4-dinitrophenyl ester + H2O - Homo sapiens 2-aminobenzoyl-RH + FK-2,4-dinitrophenyl ester - ir 395455 3.4.24.11 2-aminobenzoyl-RKFK-2,4-dinitrophenyl ester + H2O - Homo sapiens 2-aminobenzoyl-RK + FK-2,4-dinitrophenyl ester - ir 395456 3.4.24.11 2-aminobenzoyl-RNFK-2,4-dinitrophenyl ester + H2O - Homo sapiens 2-aminobenzoyl-RN + FK-2,4-dinitrophenyl ester - ir 395457 3.4.24.11 2-aminobenzoyl-RPFK-2,4-dinitrophenyl ester + H2O - Homo sapiens 2-aminobenzoyl-RP + FK-2,4-dinitrophenyl ester - ir 395458 3.4.24.11 2-aminobenzoyl-RQFK-2,4-dinitrophenyl ester + H2O - Homo sapiens 2-aminobenzoyl-RQ + FK-2,4-dinitrophenyl ester - ir 395460 3.4.24.11 2-aminobenzoyl-RRFK-2,4-dinitrophenyl amide + H2O - Homo sapiens 2-aminobenzoyl-RR + FK-2,4-dinitrophenyl amide - ir 395461 3.4.24.11 2-aminobenzoyl-RRFK-2,4-dinitrophenyl ester + H2O - Homo sapiens 2-aminobenzoyl-RR + FK-2,4-dinitrophenyl ester - ir 395462 3.4.24.11 2-aminobenzoyl-rRL-N-(2,4-dinitrophenyl)ethylenediamine + H2O - Homo sapiens 2-aminobenzoyl-D-Arg-L-Arg + Leu-N-(2,4-dinitrophenyl)ethylenediamine - ir 395464 3.4.24.11 2-aminobenzoyl-RSFK-2,4-dinitrophenyl ester + H2O - Homo sapiens 2-aminobenzoyl-RS + FK-2,4-dinitrophenyl ester - ir 395465 3.4.24.11 2-aminobenzoyl-rSL-N-(2,4-dinitrophenyl)ethylenediamine + H2O - Homo sapiens 2-aminobenzoyl-D-Arg-L-Ser + Leu-N-(2,4-dinitrophenyl)ethylenediamine - ir 395466 3.4.24.11 2-aminobenzoyl-RTFK-2,4-dinitrophenyl ester + H2O - Homo sapiens 2-aminobenzoyl-RT + FK-2,4-dinitrophenyl ester - ir 395467 3.4.24.11 2-aminobenzoyl-SRFK-2,4-dinitrophenyl ester + H2O - Homo sapiens 2-aminobenzoyl-SR + FK-2,4-dinitrophenyl ester - ir 395469 3.4.24.11 2-aminobenzoyl-TRFK-2,4-dinitrophenyl ester + H2O - Homo sapiens 2-aminobenzoyl-TR + FK-2,4-dinitrophenyl ester - ir 395470 3.4.24.11 2-aminobenzoyl-VRFK-2,4-dinitrophenyl ester + H2O - Homo sapiens 2-aminobenzoyl-VR + FK-2,4-dinitrophenyl ester - ir 395471 3.4.24.11 2-aminobenzoyl-WRFK-2,4-dinitrophenyl ester + H2O - Homo sapiens 2-aminobenzoyl-WR + FK-2,4-dinitrophenyl ester - ir 395472 3.4.24.11 2-aminobenzoyl-YRFK-2,4-dinitrophenyl ester + H2O - Homo sapiens 2-aminobenzoyl-YR + FK-2,4-dinitrophenyl ester - ir 395473 3.4.24.11 Abz-QRPRLSH-(3-nitro)Tyr + H2O - Homo sapiens Abz-QRPRL + Ser-His-(3-nitro)Tyr - ir 449463 3.4.24.11 adrenocorticotropic hormone + H2O - Lactococcus lactis ? - ? 65389 3.4.24.11 adrenomedullin + H2O - Homo sapiens ? - ? 448425 3.4.24.11 Ala-Leu-enkephalin + H2O - Oryctolagus cuniculus ? - ? 453205 3.4.24.11 Aldolase + H2O - Mus musculus ? - ? 16618 3.4.24.11 alpha-endorphin + H2O - Homo sapiens ? - ? 433404 3.4.24.11 alpha-neoendorphin + H2O - Homo sapiens ? - ? 65380 3.4.24.11 alpha-neoendorphin + H2O - Rattus norvegicus ? - ? 65380 3.4.24.11 alpha-neoendorphin + H2O - Oryctolagus cuniculus ? - ? 65380 3.4.24.11 alpha-neoendorphin + H2O - Lactococcus lactis ? - ? 65380 3.4.24.11 amyloid beta peptide + H2O - Homo sapiens ? - ? 367390 3.4.24.11 amyloid beta peptide + H2O cleavage sites, overview Homo sapiens ? - ? 367390 3.4.24.11 amyloid beta peptide + H2O degradation Homo sapiens ? - ? 367390 3.4.24.11 amyloid beta peptide Abeta42 + H2O the peptide primarily undergoes degradation by NEP in vivo in the brain Rattus norvegicus ? - ? 365803 3.4.24.11 amyloid beta peptide1-40 + H2O degradation Homo sapiens ? - ? 432662 3.4.24.11 amyloid beta peptide1-40 + H2O multiple cleavage sites Homo sapiens ? - ? 432662 3.4.24.11 amyloid beta peptide1-40 + H2O multiple cleavage sites, product peaks corresponding to Abeta1-16, Abeta1-17, Abeta10-17, Abeta20-28, Abeta20-29, and Abeta20-30. The C-terminal products result from the cleavages at K28-G29, G29-A30, and A30-I31. These products are derived from the trans-membrane region of the amyloid precursor protein (APP) from which Abeta is formed and are rather hydrophobic Homo sapiens ? - ? 432662 3.4.24.11 amyloid beta peptide1-40 + H2O the wild-type enzyme cleaves Ab1-40 predominantly at Lys16-Leu17, Leu17-Val18 and Phe19-Phe20. The mutant G399V/G714K cleaves preferentially at Phe20-Ala21 Homo sapiens amyloid beta peptide Asp1-Lys16 + amyloid beta peptide Asp1-Leu17 + amyloid beta peptide Asp1-Phe19 main cleavage fragments after 60 min with the wild-type enzyme. After 360 min these fragments are degraded further, accompanied by the appearance of Val12-Leu17 and Tyr10-Leu17 fragments ? 433416 3.4.24.11 amyloid beta peptide1-42 + H2O - Homo sapiens ? - ? 432663 3.4.24.11 amyloid beta peptide1-42 + H2O degradation Homo sapiens ? - ? 432663 3.4.24.11 amyloid beta peptide1-42 + H2O multiple cleavage sites Homo sapiens ? - ? 432663 3.4.24.11 amyloid beta(1-40) mutant A21G + H2O Flemish variant of amyloid beta. Decreased degradation by neprilysin compared to either wild-type peptide or the other mutant peptides Homo sapiens ? - ? 404301 3.4.24.11 amyloid beta(1-40) mutant D23N + H2O Iowa variant of amyloid beta Homo sapiens ? - ? 404302 3.4.24.11 amyloid beta(1-40) mutant E22G + H2O Arctic variant of amyloid beta Homo sapiens ? - ? 404303 3.4.24.11 amyloid beta(1-40) mutant E22K + H2O Italian variant of amyloid beta Homo sapiens ? - ? 404304 3.4.24.11 amyloid beta(1-40) mutant E22Q + H2O Dutch variant of amyloid beta Homo sapiens ? - ? 404305 3.4.24.11 amyloid beta(1-40) peptide + H2O - Homo sapiens ? - ? 404306 3.4.24.11 amyloid-beta + H2O - Homo sapiens ? - ? 365809 3.4.24.11 amyloid-beta + H2O degradation Homo sapiens ? - ir 365809 3.4.24.11 amyloid-beta(1-7)AAC + H2O a synthetic peptide, design and synthesis of a quenched fluorogenic peptide substrate qf-Abeta(1-7)AAC (with the sequence VHHQKAAC), which has a fluorophore, Alexa-350, linked to the side-chain of its C-terminal cysteine and a quencher, Dabcyl, linked to its N-terminus Homo sapiens ? - ? 449560 3.4.24.11 amyloid-beta(12-16)AAC + H2O a synthetic peptide, design and synthesis of a quenched fluorogenic peptide substrate qf-Abeta(12-16)AAC (with the sequence VHHQKAAC), which has a fluorophore, Alexa-350, linked to the side-chain of its C-terminal cysteine and a quencher, Dabcyl, linked to its N-terminus. This peptide emits strong fluorescence upon cleavage. qf-Abeta(12-16)AAC is more sensitive to NEP than the previously reported peptide substrates, so that concentrations of NEP as low as 0.03 nM can be detected at peptide concentration of 0.002 mM Homo sapiens ? - ? 449561 3.4.24.11 amyloid-beta1-40 + H2O degradation Mus musculus ? - ir 448433 3.4.24.11 amyloid-beta1-40 + H2O a synthetic peptide Homo sapiens ? - ? 448433 3.4.24.11 amyloid-beta1-40 + H2O - Homo sapiens Abeta1-16 + Abeta 1-17 + Abeta1-19 - ? 448434 3.4.24.11 amyloid-beta1-42 + H2O degradation Mus musculus ? - ir 448435 3.4.24.11 amyloid-beta1-42 + H2O a synthetic peptide Homo sapiens ? - ? 448435 3.4.24.11 amyloid-beta4-40 + H2O a synthetic peptide Homo sapiens ? - ? 449562 3.4.24.11 amyloid-beta4-42 + H2O a synthetic peptide Homo sapiens ? - ? 449563 3.4.24.11 angiotensin + H2O - Homo sapiens ? - ? 16268 3.4.24.11 angiotensin I + H2O - Homo sapiens ? - ? 16449 3.4.24.11 angiotensin I + H2O - Rattus norvegicus ? - ? 16449 3.4.24.11 angiotensin I + H2O - Oryctolagus cuniculus ? - ? 16449 3.4.24.11 angiotensin I + H2O - Lactococcus lactis ? - ? 16449 3.4.24.11 angiotensin I + H2O 51.6% degradation Drosophila melanogaster ? - ? 16449 3.4.24.11 angiotensin II + H2O - Homo sapiens ? - ? 16269 3.4.24.11 angiotensin II + H2O - Rattus norvegicus ? - ? 16269 3.4.24.11 angiotensin II + H2O - Oryctolagus cuniculus ? - ? 16269 3.4.24.11 angiotensin III + H2O - Rattus norvegicus ? - ? 36290 3.4.24.11 angiotensin(1-9) + H2O - Homo sapiens ? - ? 384199 3.4.24.11 Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 + H2O - Homo sapiens ? - ? 65388 3.4.24.11 Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 + H2O - Rattus norvegicus ? - ? 65388 3.4.24.11 Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 + H2O - Sus scrofa ? - ? 65388 3.4.24.11 Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 + H2O - Oryctolagus cuniculus ? - ? 65388 3.4.24.11 Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 + H2O - Lactococcus lactis ? - ? 65388 3.4.24.11 Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 + H2O i.e. substance P, seven peptides are produced Sus scrofa ? - ? 65388 3.4.24.11 Arg-vasopressin + H2O - Homo sapiens ? - ? 433428 3.4.24.11 Asp-Tyr(SO3H)-Met-Gly-Trp-Met-Asp-PheNH2 + H2O - Homo sapiens ? - ? 65361 3.4.24.11 Asp-Tyr(SO3H)-Met-Gly-Trp-Met-Asp-PheNH2 + H2O - Rattus norvegicus ? - ? 65361 3.4.24.11 Asp-Tyr(SO3H)-Met-Gly-Trp-Met-Asp-PheNH2 + H2O - Sus scrofa ? - ? 65361 3.4.24.11 Asp-Tyr(SO3H)-Met-Gly-Trp-Met-Asp-PheNH2 + H2O - Oryctolagus cuniculus ? - ? 65361 3.4.24.11 Asp-Tyr(SO3H)-Met-Gly-Trp-Met-Asp-PheNH2 + H2O i.e. cholecystokinin-8, two distinct cleavage sites: Asp-Tyr(SO3H)-Met-Gly-/-Trp-Met-Asp-/-PheNH2, the splitting of the Asp7-Phe8NH2 bond proceeds 4-times more rapidly than the Gly4-Trp5 bond Sus scrofa ? - ? 65361 3.4.24.11 Atrial natriuretic factor + H2O - Homo sapiens ? - ? 17182 3.4.24.11 Atrial natriuretic factor + H2O - Rattus norvegicus ? - ? 17182 3.4.24.11 Atrial natriuretic factor + H2O - Oryctolagus cuniculus ? - ? 17182 3.4.24.11 Atrial natriuretic factor + H2O human alpha atrial natriuretic peptide/cardiodilatin shows a single major cleavage site within the disulfide-linked loop between Cys and Phe in position 7 and 8 Canis lupus familiaris ? - ? 17182 3.4.24.11 Atrial natriuretic factor + H2O seven sites of hydrolysis: Arg4-Ser5, Cys7-Phe8, Arg11-Met12, Arg14-Ile15, Gly16-Ala17, Gly-20-Leu21, Ser25-Phe26. The initial attack takes place at a bond within the disulfide-linked loop and produces a peptide having the same amino acid composition as intact atrial natriuretic factor Homo sapiens ? - ? 17182 3.4.24.11 Atrial natriuretic factor + H2O dominant enzyme in the hydrolysis of atrial natriuretic factor Rattus norvegicus ? - ? 17182 3.4.24.11 atrial natriuretic peptide + H2O - Rattus norvegicus ? - ? 367383 3.4.24.11 atrial natriuretic peptide + H2O - Homo sapiens ? - ? 367383 3.4.24.11 atrial natriuretic peptide + H2O no activity with brain natriuretic peptide Mus musculus ? - ? 367383 3.4.24.11 azocasein + H2O - Mus musculus ? - ? 15347 3.4.24.11 Azocoll + H2O - Mus musculus ? - ? 15352 3.4.24.11 benzoyl-Gly-Gly-Arg-Leu-2-naphthylamide + H2O - Bos taurus benzoyl-Gly-Gly-Arg + L-Leu-2-naphthylamide - ? 454040 3.4.24.11 benzyloxycarbonyl-Ala-Gly-Leu-Ala + H2O - Rattus norvegicus ? - ? 65419 3.4.24.11 benzyloxycarbonyl-Phe-Arg-4-methyl-7-coumarylamide + H2O - Mus musculus ? - ? 65420 3.4.24.11 beta-amyloid peptide + H2O - Homo sapiens ? - ? 367379 3.4.24.11 beta-endorphin + H2O - Lactococcus lactis ? - ? 367385 3.4.24.11 beta-lipotropin(61-69) + H2O + H2O - Homo sapiens ? - ? 65394 3.4.24.11 beta-lipotropin(61-69) + H2O + H2O - Rattus norvegicus ? - ? 65394 3.4.24.11 beta-lipotropin(61-69) + H2O + H2O - Oryctolagus cuniculus ? - ? 65394 3.4.24.11 beta-neoendorphin + H2O - Homo sapiens ? - ? 64950 3.4.24.11 beta-neoendorphin + H2O - Rattus norvegicus ? - ? 64950 3.4.24.11 beta-neoendorphin + H2O - Oryctolagus cuniculus ? - ? 64950 3.4.24.11 beta-neoendorphin + H2O - Lactococcus lactis ? - ? 64950 3.4.24.11 bradykinin + H2O - Homo sapiens ? - ? 16889 3.4.24.11 bradykinin + H2O - Rattus norvegicus ? - ? 16889 3.4.24.11 bradykinin + H2O - Sus scrofa ? - ? 16889 3.4.24.11 bradykinin + H2O - Oryctolagus cuniculus ? - ? 16889 3.4.24.11 bradykinin + H2O - Lactococcus lactis ? - ? 16889 3.4.24.11 bradykinin + H2O 27.8% degradation Drosophila melanogaster ? - ? 16889 3.4.24.11 brain natriuretic peptide + H2O - Homo sapiens ? - ? 448485 3.4.24.11 casein + H2O - Mus musculus ? - ? 15445 3.4.24.11 cholecystokinin-8 + H2O - Homo sapiens ? - ? 64952 3.4.24.11 cholecystokinin-8 + H2O - Sus scrofa ? - ? 64952 3.4.24.11 D-Ala2-Leu5-enkephalin + H2O i.e. Tyr-D-Ala-Gly-Phe-Leu Mus musculus Tyr-D-Ala-Gly + Phe-Leu - ? 65372 3.4.24.11 D-Ala2-Leu5-enkephalin + H2O i.e. Tyr-D-Ala-Gly-Phe-Leu Homo sapiens Tyr-D-Ala-Gly + Phe-Leu - ? 65372 3.4.24.11 D-Ala2-Leu5-enkephalin + H2O i.e. Tyr-D-Ala-Gly-Phe-Leu Rattus norvegicus Tyr-D-Ala-Gly + Phe-Leu - ? 65372 3.4.24.11 D-Ala2-Leu5-enkephalin + H2O i.e. Tyr-D-Ala-Gly-Phe-Leu Sus scrofa Tyr-D-Ala-Gly + Phe-Leu - ? 65372 3.4.24.11 D-Ala2-Leu5-enkephalin + H2O i.e. Tyr-D-Ala-Gly-Phe-Leu Canis lupus familiaris Tyr-D-Ala-Gly + Phe-Leu - ? 65372 3.4.24.11 D-Ala2-Leu5-enkephalin + H2O i.e. Tyr-D-Ala-Gly-Phe-Leu Ascaris suum Tyr-D-Ala-Gly + Phe-Leu - ? 65372 3.4.24.11 D-Ala2-Leu5-enkephalin + H2O - Oryctolagus cuniculus ? - ? 453206 3.4.24.11 D-Ala2-Leu5-enkephalinamide + H2O - Sus scrofa ? - ? 65373 3.4.24.11 dansyl-Gly-Trp-Gly + H2O - Rattus norvegicus dansyl-Gly + Trp-Gly - ? 65413 3.4.24.11 dansyl-Gly-Tyr-Gly + H2O - Rattus norvegicus dansyl-Gly + Tyr-Gly - ? 65414 3.4.24.11 dansyl-Gly-Tyr-Gly-NH2 + H2O - Rattus norvegicus dansyl-Gly + Tyr-Gly-NH2 - ? 65415 3.4.24.11 dynorphin + H2O - Homo sapiens ? - ? 64951 3.4.24.11 dynorphin + H2O - Rattus norvegicus ? - ? 64951 3.4.24.11 dynorphin + H2O - Oryctolagus cuniculus ? - ? 64951 3.4.24.11 dynorphin + H2O dynorphin A-6, A-8, A-9, A-10, A-13 and A-17 Lactococcus lactis ? - ? 64951 3.4.24.11 dynorphin A-10 + H2O - Lactococcus lactis ? - ? 453198 3.4.24.11 dynorphin A-13 + H2O - Lactococcus lactis ? - ? 453199 3.4.24.11 dynorphin A-17 + H2O - Lactococcus lactis ? - ? 367394 3.4.24.11 dynorphin A-6 + H2O - Lactococcus lactis ? - ? 453200 3.4.24.11 dynorphin A-8 + H2O - Lactococcus lactis ? - ? 453201 3.4.24.11 dynorphin A-9 + H2O - Lactococcus lactis ? - ? 453202 3.4.24.11 dynorphin(1-9) + H2O - Sus scrofa ? - ? 65379 3.4.24.11 Elastin + H2O - Homo sapiens ? - ? 16576 3.4.24.11 Elastin + H2O - Rattus norvegicus ? - ? 16576 3.4.24.11 Elastin + H2O - Mus musculus ? - ? 16576 3.4.24.11 endothelin-1 + H2O - Homo sapiens ? - ? 367555 3.4.24.11 enkephalin + H2O - Lactococcus lactis ? - ? 36285 3.4.24.11 enkephalin + H2O - Homo sapiens ? - ? 36285 3.4.24.11 enkephalin + H2O hydrolysis at Gly3-Phe4 Homo sapiens ? - ? 36285 3.4.24.11 enkephalin + H2O hydrolysis at Gly3-Phe4 Rattus norvegicus ? - ? 36285 3.4.24.11 enkephalin + H2O hydrolysis at Gly3-Phe4 Oryctolagus cuniculus ? - ? 36285 3.4.24.11 exendin-4 + H2O poor substrate Homo sapiens ? - ? 65407 3.4.24.11 fibrinogen + H2O inactivation, mechanism of NEP-mediated inactivation of fibrinogen through cleavage of the N-termini of the Aalpha- and Bbeta-chains of fibrinogen thereby significantly impairing initiation of fibrin formation by thrombin, overview Homo sapiens fibrin + ? - ? 36912 3.4.24.11 fibrinogen + H2O fibrinogen from human and rat blood plasma, inactivation Homo sapiens fibrin + ? - ? 36912 3.4.24.11 FMRF amide + H2O - Homo sapiens ? - ? 65400 3.4.24.11 FMRF amide + H2O - Rattus norvegicus ? - ? 65400 3.4.24.11 FMRF amide + H2O - Oryctolagus cuniculus ? - ? 65400 3.4.24.11 galanin + H2O - Mus musculus ? - ? 412071 3.4.24.11 gamma-endorphin + H2O - Homo sapiens ? - ? 65382 3.4.24.11 gamma-endorphin + H2O - Rattus norvegicus ? - ? 65382 3.4.24.11 gamma-endorphin + H2O - Oryctolagus cuniculus ? - ? 65382 3.4.24.11 gastri-releasing peptide + H2O - Homo sapiens ? - ? 433772 3.4.24.11 gastric inhibitor peptide + H2O poor substrate Homo sapiens ? - ? 65408 3.4.24.11 gastrin + H2O - Homo sapiens ? - ? 65359 3.4.24.11 gastrin + H2O - Rattus norvegicus ? - ? 65359 3.4.24.11 gastrin + H2O - Oryctolagus cuniculus ? - ? 65359 3.4.24.11 gastrin G-17 + H2O - Homo sapiens ? - ? 65358 3.4.24.11 gastrin G-17 + H2O cleavage at four sites, Trp4-Leu5, Ala11-Tyr12, Gly13-Trp14 and Asp16-Phe17 Homo sapiens ? - ? 65358 3.4.24.11 gastrin G-17 + H2O sulfated and unsulfated gastrin, cleavage is faster with the sulfated peptide Homo sapiens ? - ? 65358 3.4.24.11 gastrin releasing peptide-10 + H2O - Homo sapiens ? - ? 65360 3.4.24.11 gastrin releasing peptide-10 + H2O - Rattus norvegicus ? - ? 65360 3.4.24.11 gastrin releasing peptide-10 + H2O - Oryctolagus cuniculus ? - ? 65360 3.4.24.11 GLP-1 + H2O - Homo sapiens ? - ? 364905 3.4.24.11 GLP-1(7-36)amide + H2O insulinotropic peptide hormone Homo sapiens ? - ? 65409 3.4.24.11 Glucagon + H2O - Homo sapiens ? - ? 15578 3.4.24.11 Glucagon + H2O - Sus scrofa ? - ? 15578 3.4.24.11 Glucagon + H2O NEP24.11 is an important mediator of the degradation of both endogenous and exogenous glucagon in vivo Sus scrofa ? - ? 15578 3.4.24.11 glucagon-like peptide 1 + H2O - Mus musculus ? - ? 364897 3.4.24.11 glutaryl-Ala-Ala-Phe-2-naphthylamide + H2O - Bos taurus glutaryl-Ala-Ala + Phe-2-naphthylamide - ? 65430 3.4.24.11 glutaryl-Ala-Ala-Phe-4-methoxy-2-naphthylamide + H2O - Bos taurus glutaryl-Ala-Ala + Phe-4-methoxy-2-naphthylamide - ? 65429 3.4.24.11 glutaryl-Ala-Ala-Phe-4-methoxy-2-naphthylamide + H2O - Homo sapiens glutaryl-Ala-Ala + Phe-4-methoxy-2-naphthylamide - ? 65429 3.4.24.11 glutaryl-Ala-Ala-Phe-4-methoxy-2-naphthylamide + H2O - Rattus norvegicus glutaryl-Ala-Ala + Phe-4-methoxy-2-naphthylamide - ? 65429 3.4.24.11 glutaryl-Ala-Ala-Phe-4-methoxy-2-naphthylamide + H2O - Mus musculus glutaryl-Ala-Ala + Phe-4-methoxy-2-naphthylamide - ? 65429 3.4.24.11 glutaryl-Ala-Ala-Phe-4-methoxy-2-naphthylamine + H2O - Rattus norvegicus ? - ? 65433 3.4.24.11 glutaryl-Gly-Gly-Phe-2-naphthylamide + H2O - Sus scrofa glutaryl-Gly-Gly + Phe-2-naphthylamide - ? 65434 3.4.24.11 glutaryl-Gly-Gly-Phe-2-naphthylamide + H2O - Rattus norvegicus glutaryl-Gly-Gly-Phe + 2-naphthylamine - ? 397279 3.4.24.11 Gly-Trp-Gly + H2O - Rattus norvegicus ? - ? 65418 3.4.24.11 haemoglobin + H2O - Mus musculus ? - ? 65404 3.4.24.11 hippuryl-Arg-Arg-Ala-2-naphthylamide + H2O - Rattus norvegicus hippuryl-Arg-Arg + Ala-2-naphthylamide - ? 65368 3.4.24.11 hippuryl-Arg-Arg-Leu-2-naphthylamide + H2O - Rattus norvegicus hippuryl-Arg-Arg + Leu-2-naphthylamide - ? 65367 3.4.24.11 hippuryl-Arg-Arg-Leu-2-naphthylamide + H2O - Bos taurus hippuryl-Arg-Arg + Leu-2-naphthylamide - ? 65367 3.4.24.11 His-Lys-Thr-Asp-Ser-Phe-Val-Gly-Leu-Met-NH2 + H2O - Homo sapiens His-Lys-Thr-Asp-Ser + Phe-Val-Gly + Leu-Met-NH2 - ? 65391 3.4.24.11 His-Lys-Thr-Asp-Ser-Phe-Val-Gly-Leu-Met-NH2 + H2O - Rattus norvegicus His-Lys-Thr-Asp-Ser + Phe-Val-Gly + Leu-Met-NH2 - ? 65391 3.4.24.11 His-Lys-Thr-Asp-Ser-Phe-Val-Gly-Leu-Met-NH2 + H2O - Sus scrofa His-Lys-Thr-Asp-Ser + Phe-Val-Gly + Leu-Met-NH2 - ? 65391 3.4.24.11 His-Lys-Thr-Asp-Ser-Phe-Val-Gly-Leu-Met-NH2 + H2O - Oryctolagus cuniculus His-Lys-Thr-Asp-Ser + Phe-Val-Gly + Leu-Met-NH2 - ? 65391 3.4.24.11 His-Lys-Thr-Asp-Ser-Phe-Val-Gly-Leu-Met-NH2 + H2O i.e. neurokinin A, hydrolysis at two sites: Ser5-Phe6 and Gly8-Leu9 Sus scrofa His-Lys-Thr-Asp-Ser + Phe-Val-Gly + Leu-Met-NH2 - ? 65391 3.4.24.11 insulin + H2O - Homo sapiens ? - ? 36836 3.4.24.11 insulin B chain + H2O - Homo sapiens ? - ? 37184 3.4.24.11 insulin B chain + H2O - Rattus norvegicus ? - ? 37184 3.4.24.11 insulin B chain + H2O - Oryctolagus cuniculus ? - ? 37184 3.4.24.11 insulin B chain + H2O multiple cleavage sites Homo sapiens ? - ? 37184 3.4.24.11 insulin B chain + H2O multiple cleavage sites, product analysis and cleavage sites preferences of wild-type and mutant neprilysins, cleavage sites, overview Homo sapiens ? - ? 37184 3.4.24.11 interleukin 1beta + H2O - Homo sapiens ? - ? 65398 3.4.24.11 interleukin 1beta + H2O - Rattus norvegicus ? - ? 65398 3.4.24.11 interleukin 1beta + H2O - Oryctolagus cuniculus ? - ? 65398 3.4.24.11 Leu-2-naphthylamide + H2O - Mus musculus ? - ? 65432 3.4.24.11 Leu-enkephalin + H2O - Lactococcus lactis ? - ? 116056 3.4.24.11 Leu5-enkephalin + H2O - Homo sapiens ? - ? 65374 3.4.24.11 Leu5-enkephalin + H2O - Rattus norvegicus ? - ? 65374 3.4.24.11 Leu5-enkephalin + H2O - Sus scrofa ? - ? 65374 3.4.24.11 Leu5-enkephalin + H2O - Bos taurus ? - ? 65374 3.4.24.11 Leu5-enkephalin + H2O - Oryctolagus cuniculus ? - ? 65374 3.4.24.11 Leu5-enkephalin-Arg6 + H2O - Sus scrofa ? - ? 65376 3.4.24.11 Leu5-enkephalinamide + H2O - Sus scrofa ? - ? 65375 3.4.24.11 Leu5-enkephalinamide + H2O - Lactococcus lactis ? - ? 65375 3.4.24.11 leucine5-enkephalin + H2O Tyr-Gly-Gly-Phe-Leu is cleaved at the Gly-Phe bond by the wild-type enzyme Homo sapiens ? - ? 432830 3.4.24.11 Luliberin + H2O poor substrate Sus scrofa ? - ? 16614 3.4.24.11 Luteinizing hormone-releasing hormone + H2O - Homo sapiens ? - ? 17065 3.4.24.11 Luteinizing hormone-releasing hormone + H2O - Rattus norvegicus ? - ? 17065 3.4.24.11 Luteinizing hormone-releasing hormone + H2O - Sus scrofa ? - ? 17065 3.4.24.11 Luteinizing hormone-releasing hormone + H2O - Oryctolagus cuniculus ? - ? 17065 3.4.24.11 Mca-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys-(Dnp)-OH + H2O - Homo sapiens ? - ? 450267 3.4.24.11 Mca-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys-(Dnp)-OH + H2O - Mus musculus ? - ? 450267 3.4.24.11 Mca-RPPGFSAFK-(Dnp) + H2O - Homo sapiens ? - ? 450270 3.4.24.11 Met-enkephalin + H2O - Lactococcus lactis ? - ? 116055 3.4.24.11 Met-enkephalin amide + H2O - Lactococcus lactis ? - ? 116057 3.4.24.11 Met-enkephalin-Arg6-Gly7-Leu + H2O - Homo sapiens ? - ? 65371 3.4.24.11 Met-enkephalin-Arg6-Gly7-Leu + H2O - Rattus norvegicus ? - ? 65371 3.4.24.11 Met-enkephalin-Arg6-Gly7-Leu + H2O - Oryctolagus cuniculus ? - ? 65371 3.4.24.11 Met-Leu-Phe + H2O chemotactic peptide Homo sapiens ? - ? 65397 3.4.24.11 Met-Leu-Phe + H2O chemotactic peptide Rattus norvegicus ? - ? 65397 3.4.24.11 Met-Leu-Phe + H2O chemotactic peptide Oryctolagus cuniculus ? - ? 65397 3.4.24.11 Met5-enkephalin-Arg6 + H2O - Sus scrofa ? - ? 65370 3.4.24.11 Met5-enkephalin-Arg6-Phe7 + H2O - Homo sapiens ? - ? 65369 3.4.24.11 Met5-enkephalin-Arg6-Phe7 + H2O - Rattus norvegicus ? - ? 65369 3.4.24.11 Met5-enkephalin-Arg6-Phe7 + H2O - Sus scrofa ? - ? 65369 3.4.24.11 Met5-enkephalin-Arg6-Phe7 + H2O - Oryctolagus cuniculus ? - ? 65369 3.4.24.11 additional information - Homo sapiens ? - ? 89 3.4.24.11 additional information - Sus scrofa ? - ? 89 3.4.24.11 additional information could play an important role in the hydrolysis of neuropeptides Sus scrofa ? - ? 89 3.4.24.11 additional information physiological role in metabolism of insect peptides at the synapse Schistocerca gregaria ? - ? 89 3.4.24.11 additional information the enzyme may play an important role as regulator of plasma-derived peptides in the nephron Canis lupus familiaris ? - ? 89 3.4.24.11 additional information elevation of the enzyme activity in serum from underground coal miners exposed to chronic inhalation of coal mine dust particles. The enzyme reflects the chronic pulmonary inflammatory state induced by coalmine dust exposure, and so may be a marker of lung injury Homo sapiens ? - ? 89 3.4.24.11 additional information the enzyme is involved in the processing of other peptide hormones Homo sapiens ? - ? 89 3.4.24.11 additional information the enzyme is responsible for the difference in metabolism of sulfated and unsulfated gastrin in human circulation Homo sapiens ? - ? 89 3.4.24.11 additional information the enzyme is the major inactivator of enkephalin in brain Homo sapiens ? - ? 89 3.4.24.11 additional information the enzyme is the major inactivator of enkephalin in brain Rattus norvegicus ? - ? 89 3.4.24.11 additional information the enzyme is the major inactivator of enkephalin in brain Oryctolagus cuniculus ? - ? 89 3.4.24.11 additional information the enzyme may participate in the regulation of blood pressure and water and sodium excretion through inactivation of kinins Homo sapiens ? - ? 89 3.4.24.11 additional information the enzyme may participate in the regulation of blood pressure and water and sodium excretion through inactivation of kinins Rattus norvegicus ? - ? 89 3.4.24.11 additional information the enzyme may participate in the regulation of blood pressure and water and sodium excretion through inactivation of kinins Sus scrofa ? - ? 89 3.4.24.11 additional information the enzyme may participate in the regulation of blood pressure and water and sodium excretion through inactivation of kinins Oryctolagus cuniculus ? - ? 89 3.4.24.11 additional information catabolism of atrial and brain natriuretic peptide is independent of neutral endopeptidase. One or more other peptidase degrade atrial natriuretic peptide or brain natriuretic peptide in the heart, lungs and kidney Mus musculus ? - ? 89 3.4.24.11 additional information in sepsis, the local concentration and action of adrenomedullin in tissue may be differentially regulated by NEP Rattus norvegicus ? - ? 89 3.4.24.11 additional information induction of neutral endopeptidase (NEP) activity of SK-N-SH cells by natural compounds from green tea. Caffeine leads to an increase in specific cellular neutral endopeptidase activity more than theophylline, theobromine or theanine. The combination of epigallocatechin and epigallocatechingallate with caffeine, theobromine or theophylline induces cellular neutral endopeptidase activity. The enhancement of cellular neutral endopeptidase activity by green tea extract and its natural products might be correlated with an elevated level of intracellular cyclic adenosine monophosphate Homo sapiens ? - ? 89 3.4.24.11 additional information NEP-1 plays an important role in the regulation of nematode locomation. NEP-1 is a central component that controls the neuronal innervation of pharyngeal pumping in Caenorhabditis elegans Caenorhabditis elegans ? - ? 89 3.4.24.11 additional information neutral endopeptidase 24.11/CD10 suppresses the progression of ovarian carcinomas Homo sapiens ? - ? 89 3.4.24.11 additional information neutral endopeptidase inhibition has natriuretic and aquaretic actions in cirrhosis without any effect on blood pressure and kidney perfusion due to a significant overexpression of thgis enzyme in renal cortex Rattus norvegicus ? - ? 89 3.4.24.11 additional information nicastrin controls neprilysin at a transcriptional level by contributing to the production of the beta-amyloid precursor protein intracellular domain with the gamma-secretase complex Mus musculus ? - ? 89 3.4.24.11 additional information no activity with angiotensin (1-7) Homo sapiens ? - ? 89 3.4.24.11 additional information hydrolysis of polypeptides between hydrophobic residues Homo sapiens ? - ? 89 3.4.24.11 additional information a quenched fluorogenic peptide substrate containing the first seven residues of the Abeta peptide plus a C-terminal Cysteine residue is synthesized to detect neprilysin activity. A fluorophore is attached to the C-terminal Cysteine and its fluorescence is quenched by a quencher linked to the N-terminus of the peptide Homo sapiens ? - ? 89 3.4.24.11 additional information the enzyme degrades a wide range of peptide substrates, physiologically relevant peptides are such as enkephalins, tachykinins, and natriuretic peptides Homo sapiens ? - ? 89 3.4.24.11 additional information the enzyme directly interacts with phosphatidylserine and cardiolipin but not with cholesterol Homo sapiens ? - ? 89 3.4.24.11 additional information the enzyme has broader substrate specificity and is a peptidase capable of cleaving a variety of physiological peptides Homo sapiens ? - ? 89 3.4.24.11 additional information human neprilysin-2 has a more restricted substrate specificity compared to human neprilysin with less activity against several vasoactive peptides Homo sapiens ? - ? 89 3.4.24.11 additional information activity measurement optimization using the synthetic fluorogenic peptide substrates, evaluation and kinetics, overview Homo sapiens ? - - 89 3.4.24.11 additional information activity measurement optimization using the synthetic fluorogenic peptide substrates, evaluation and kinetics, overview Homo sapiens ? - ? 89 3.4.24.11 additional information analysis of intact amyloid-beta peptides and their NEP cleavage products by mass spectrometry. 73% of amyloid-beta4-40, 31% of amyloid-beta4-42, 45% of amyloid-beta1-40, and 18% of amyloid-beta1-42 degradation resulting from NEP catalyzed hydrolysis. Amyloid-beta4-9/amyloid-beta1-9, amyloid-beta10-17, and amyloid-beta20-30 are the major enzymatic products Homo sapiens ? - - 89 3.4.24.11 additional information analysis of intact amyloid-beta peptides and their NEP cleavage products by mass spectrometry. 73% of amyloid-beta4-40, 31% of amyloid-beta4-42, 45% of amyloid-beta1-40, and 18% of amyloid-beta1-42 degradation resulting from NEP catalyzed hydrolysis. Amyloid-beta4-9/amyloid-beta1-9, amyloid-beta10-17, and amyloid-beta20-30 are the major enzymatic products Homo sapiens ? - ? 89 3.4.24.11 additional information in vitro NEP proteolysis generates fragments that lack the ability to bind to the apelin receptor, NEP fully inactivates apelin Homo sapiens ? - - 89 3.4.24.11 additional information in vitro NEP proteolysis generates fragments that lack the ability to bind to the apelin receptor, NEP fully inactivates apelin Homo sapiens ? - ? 89 3.4.24.11 additional information murine amyloid-beta has a far lower propensity to aggregate than human amyloid-beta. Amyloid-beta degradation products formed during NEP incubation are identified by mass spectrometry, analysis of predominant NEP cleavage sites detected by mass spectrometry for human and murine amyloid-beta1-42 and amyloid-beta.4-15. In addition to the amyloid-beta4-9 fragment, di- and tetra-peptides amyloid-beta18-19 and amyloid-beta39-42 are also observed Homo sapiens ? - - 89 3.4.24.11 additional information murine amyloid-beta has a far lower propensity to aggregate than human amyloid-beta. Amyloid-beta degradation products formed during NEP incubation are identified by mass spectrometry, analysis of predominant NEP cleavage sites detected by mass spectrometry for human and murine amyloid-beta1-42 and amyloid-beta.4-15. In addition to the amyloid-beta4-9 fragment, di- and tetra-peptides amyloid-beta18-19 and amyloid-beta39-42 are also observed Homo sapiens ? - ? 89 3.4.24.11 additional information NEP degrades murine amyloid-beta faster than human amyloid-beta, observed with full-length amyloid-beta containing 40 or 42 amino acids (Abeta1-40 and Abeta1-42) and a truncated form (Abeta4-15), which contains one of the main NEP cleavage sites for amyloid-beta (between positions 9 and 10), harbours all three amino acid differences between murine and human amyloid-beta sequences, and is less prone to aggregation and thus might be a simpler model to investigate amyloid-beta biochemistry. Murine amyloid-beta has a far lower propensity to aggregate than human amyloid-beta. Amyloid-beta degradation products formed during NEP incubation are identified by mass spectrometry, analysis of predominant NEP cleavage sites detected by mass spectrometry for human and murine amyloid-beta1-42 and amyloid-beta.4-15. In addition to the amyloid-beta4-9 fragment, di- and tetra-peptides amyloid-beta18-19 and amyloid-beta39-42 are also observed Mus musculus ? - - 89 3.4.24.11 additional information NEP degrades murine amyloid-beta faster than human amyloid-beta, observed with full-length amyloid-beta containing 40 or 42 amino acids (Abeta1-40 and Abeta1-42) and a truncated form (Abeta4-15), which contains one of the main NEP cleavage sites for amyloid-beta (between positions 9 and 10), harbours all three amino acid differences between murine and human amyloid-beta sequences, and is less prone to aggregation and thus might be a simpler model to investigate amyloid-beta biochemistry. Murine amyloid-beta has a far lower propensity to aggregate than human amyloid-beta. Amyloid-beta degradation products formed during NEP incubation are identified by mass spectrometry, analysis of predominant NEP cleavage sites detected by mass spectrometry for human and murine amyloid-beta1-42 and amyloid-beta.4-15. In addition to the amyloid-beta4-9 fragment, di- and tetra-peptides amyloid-beta18-19 and amyloid-beta39-42 are also observed Mus musculus ? - ? 89 3.4.24.11 N-(4-carboxy-1-oxobutyl)-L-alanyl-L-alanyl-N-(4-methoxy-2-naphthalenyl)-L-phenylalaninamide + H2O - Homo sapiens N-(4-carboxy-1-oxobutyl)-L-alanyl-L-alanyl-L-phenylalanine + 4-methoxy-2-naphthylamine - ? 433966 3.4.24.11 N-acetyl-Gly-Trp-Gly + H2O - Rattus norvegicus N-acetyl-Gly + Trp-Gly - ? 65417 3.4.24.11 N-benzyloxycarbonyl-Gly-Gly-Leu 2-naphthylamide + H2O - Bos taurus N-benzyloxycarbonyl-Gly-Gly + L-leucine 2-naphthylamide - ? 65431 3.4.24.11 N-benzyoxycarbonyl-Gly-Gly-Leu 2-naphthylamide + H2O - Bos taurus ? - ? 453203 3.4.24.11 N-benzyoxycarbonyl-Gly-Gly-Leu-2-naphthylamide + H2O - Bos taurus N-benzyoxycarbonyl-Gly-Gly + L-Leu-2-naphthylamide - ? 454041 3.4.24.11 N-dansyl-Ala-Gly-D-(4-nitro-Phe)-Gly + H2O - Rattus norvegicus ? - ? 418888 3.4.24.11 N-dansyl-D-Ala-Gly-p-nitrophenyl-Ala-Gly + H2O - Homo sapiens ? - ? 65416 3.4.24.11 N-dansyl-D-Ala-Gly-p-nitrophenyl-Ala-Gly + H2O - Bos taurus ? - ? 65416 3.4.24.11 N-formyl-L-Met-Leu-Phe + H2O the enzyme may play an important role in modulating chemotactic response by cleavage of the chemotactic substance N-formyl-Met-Leu-Phe Homo sapiens ? - ? 369677 3.4.24.11 N-Formyl-Met-Leu-Phe + H2O - Homo sapiens N-Formyl-Met + Leu-Phe - ? 15158 3.4.24.11 Na,K-ATPase alpha subunit + H2O - Oryctolagus cuniculus ? - ? 418015 3.4.24.11 Nalpha-benzoyl-Gly-Arg-Arg-Ala-2-naphthylamide + H2O - Bos taurus Nalpha-benzoyl-Gly-Arg-Arg + Ala-2-naphthylamide - ? 65423 3.4.24.11 Nalpha-benzoyl-Gly-Arg-Arg-Leu-2-naphthylamide + H2O - Bos taurus Nalpha-benzoyl-Gly-Arg-Arg + Leu-2-naphthylamide - ? 65421 3.4.24.11 Nalpha-benzoyl-Gly-Arg-Arg-Phe-2-naphthylamide + H2O - Bos taurus Nalpha-benzoyl-Gly-Arg-Arg + Phe-2-naphthylamide - ? 65422 3.4.24.11 Nalpha-benzoyl-Gly-Arg-Leu-2-naphthylamide + H2O - Bos taurus Nalpha-benzoyl-Gly-Arg + Leu-2-naphthylamide - ? 65424 3.4.24.11 Nalpha-benzoyl-Gly-Gly-Arg-Leu-2-naphthylamide + H2O - Bos taurus Nalpha-benzoyl-Gly-Gly-Arg + Leu-2-naphthylamide - ? 65427 3.4.24.11 Nalpha-benzoyl-Gly-Lys-Arg-Arg-Leu-2-naphthylamide + H2O - Bos taurus Nalpha-benzoyl-Gly-Lys-Arg-Arg + Leu-2-naphthylamide - ? 65425 3.4.24.11 Nalpha-benzoyl-Gly-Lys-Lys-Arg-Arg-Leu-2-naphthylamide + H2O - Bos taurus Nalpha-benzoyl-Gly-Lys-Lys-Arg-Arg + Leu-2-naphthylamide - ? 65426 3.4.24.11 Neurokinin A + H2O - Homo sapiens ? - ? 17209 3.4.24.11 neurokinin B + H2O - Homo sapiens ? - ? 65392 3.4.24.11 neurokinin B + H2O - Rattus norvegicus ? - ? 65392 3.4.24.11 neurokinin B + H2O - Oryctolagus cuniculus ? - ? 65392 3.4.24.11 neuropeptide Y + H2O - Homo sapiens truncated neuropeptide Y + C-terminal fragments of neuropeptide Y neuropeptide Y 21-36 and 31-36 are the most abundant fragments generated in vivo ? 402791 3.4.24.11 neurotensin + H2O - Homo sapiens ? - ? 15385 3.4.24.11 neurotensin + H2O - Rattus norvegicus ? - ? 15385 3.4.24.11 neurotensin + H2O - Sus scrofa ? - ? 15385 3.4.24.11 neurotensin + H2O - Oryctolagus cuniculus ? - ? 15385 3.4.24.11 neurotensin + H2O cleaves at Pro10-Tyr11 and Tyr11-Ile12 Homo sapiens ? - ? 15385 3.4.24.11 nociceptin + H2O - Homo sapiens ? - ? 434068 3.4.24.11 oxytocin + H2O - Homo sapiens ? - ? 65396 3.4.24.11 oxytocin + H2O - Rattus norvegicus ? - ? 65396 3.4.24.11 oxytocin + H2O - Oryctolagus cuniculus ? - ? 65396 3.4.24.11 pBNP-26 + H2O cleaved at several sites Canis lupus familiaris ? - ? 65357 3.4.24.11 physalaemin + H2O - Homo sapiens ? - ? 37232 3.4.24.11 physalaemin + H2O - Rattus norvegicus ? - ? 37232 3.4.24.11 physalaemin + H2O - Sus scrofa ? - ? 37232 3.4.24.11 physalaemin + H2O - Oryctolagus cuniculus ? - ? 37232 3.4.24.11 pulmonary vasodilative vasoactive intestinal peptide + H2O rapid inactivation Homo sapiens ? - ? 432919 3.4.24.11 pyroglutamyl-Leu-Asn-Phe-Thr-Pro-Asn-Trp-Gly-Thr-NH2 + H2O - Sus scrofa pyroglutamyl-Leu-Asn + Phe-Thr-Pro-ASn-Trp-Gly-Thr-NH2 - ? 65412 3.4.24.11 pyroglutamyl-Leu-Asn-Phe-Thr-Pro-Asn-Trp-Gly-Thr-NH2 + H2O - Ascaris suum pyroglutamyl-Leu-Asn + Phe-Thr-Pro-ASn-Trp-Gly-Thr-NH2 - ? 65412 3.4.24.11 pyroglutamyl-Leu-Asn-Phe-Thr-Pro-Asn-Trp-Gly-Thr-NH2 + H2O - Schistocerca gregaria pyroglutamyl-Leu-Asn + Phe-Thr-Pro-ASn-Trp-Gly-Thr-NH2 - ? 65412 3.4.24.11 Secretin + H2O - Homo sapiens ? - ? 17174 3.4.24.11 somatostatin 14 + H2O - Homo sapiens ? - ? 36886 3.4.24.11 somatostatin 28 + H2O - Homo sapiens ? - ? 434401 3.4.24.11 striatal natriuretic factor + H2O - Rattus norvegicus ? - ? 65355 3.4.24.11 Substance P + H2O - Homo sapiens ? - ? 17205 3.4.24.11 Substance P + H2O - Rattus norvegicus ? - ? 17205 3.4.24.11 Substance P + H2O - Sus scrofa ? - ? 17205 3.4.24.11 Substance P + H2O - Lactococcus lactis ? - ? 17205 3.4.24.11 Substance P + H2O cleaves at Gln6-Phe7, Phe7-Phe8, and Gly9-Leu10 Homo sapiens ? - ? 17205 3.4.24.11 Substance P + H2O 85.8% degradation Drosophila melanogaster ? - ? 17205 3.4.24.11 succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide + H2O - Mus musculus succinyl-Ala-Ala + Phe-4-methylcoumarin 7-amide - ? 65437 3.4.24.11 succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide + H2O - Homo sapiens succinyl-Ala-Ala + Phe-4-methylcoumarin 7-amide - ? 65437 3.4.24.11 succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide + H2O - Rattus norvegicus succinyl-Ala-Ala + Phe-4-methylcoumarin 7-amide - ? 65437 3.4.24.11 succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O - Mus musculus ? - ? 95054 3.4.24.11 succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O - Homo sapiens ? - ? 95054 3.4.24.11 succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O - Rattus norvegicus succinyl-Ala-Ala-Phe + 7-amino-4-methylcoumarin - ? 366428 3.4.24.11 succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O - Rattus norvegicus Wistar-Hannover succinyl-Ala-Ala-Phe + 7-amino-4-methylcoumarin - ? 366428 3.4.24.11 succinyl-Ala-Ala-Phe-p-nitroanilide + H2O - Homo sapiens ? - ? 36852 3.4.24.11 succinyl-Arg-Arg-Leu-2-naphthylamide + H2O - Bos taurus succinyl-Arg-Arg + Leu-2-naphthylamide - ? 65428 3.4.24.11 succinyl-Arg-Pro-Phe-His-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O - Lactococcus lactis ? - ? 65436 3.4.24.11 sulfated cholecystokinin octapeptide + H2O - Homo sapiens ? - ? 453204 3.4.24.11 tachykinin + H2O 27.8% degradation Drosophila melanogaster ? - ? 412946 3.4.24.11 Tyr-D-Ala-Gly-Phe-Leu + H2O - Sus scrofa Tyr-D-Ala-Gly + Phe-Leu - ? 65435 3.4.24.11 Tyr-D-Ala-Gly-Phe-Met + H2O - Rattus norvegicus Tyr-D-Ala-Gly + Phe-Met - ? 65365 3.4.24.11 Tyr-D-Ala-Gly-Phe-Met + H2O - Sus scrofa Tyr-D-Ala-Gly + Phe-Met - ? 65365 3.4.24.11 Tyr-D-Ala-Gly-Phe-Met-NH2 + H2O - Mus musculus Tyr-D-Ala-Gly + Phe-Met-NH2 - ? 65364 3.4.24.11 Tyr-D-Ala-Gly-Phe-Met-NH2 + H2O - Rattus norvegicus Tyr-D-Ala-Gly + Phe-Met-NH2 - ? 65364 3.4.24.11 Tyr-D-Ala-Gly-Phe-Met-NH2 + H2O - Sus scrofa Tyr-D-Ala-Gly + Phe-Met-NH2 - ? 65364 3.4.24.11 Tyr-Gly-Gly-Phe-Met + H2O i.e. methionine enkephalin Homo sapiens Tyr-Gly-Gly + Phe-Met - ? 65366 3.4.24.11 Tyr-Gly-Gly-Phe-Met + H2O i.e. methionine enkephalin Rattus norvegicus Tyr-Gly-Gly + Phe-Met - ? 65366 3.4.24.11 Tyr-Gly-Gly-Phe-Met + H2O i.e. methionine enkephalin Sus scrofa Tyr-Gly-Gly + Phe-Met - ? 65366 3.4.24.11 Tyr-Gly-Gly-Phe-Met + H2O i.e. methionine enkephalin Bos taurus Tyr-Gly-Gly + Phe-Met - ? 65366 3.4.24.11 Vasoactive intestinal peptide + H2O - Lactococcus lactis ? - ? 15398 3.4.24.11 Z-Ala-Ala-Leu-4-nitroanilide + H2O - Homo sapiens Z-Ala-Ala-Leu + 4-nitroaniline - ? 367581