3.4.23.35	alpha Factor + H2O	cleavage site: Leu6-Lys7	Saccharomyces cerevisiae	Trp-His-Trp-Leu-Gln-Leu + Lys-Pro-Gly-Gln-Pro-Met-Tyr	-	?	16501	
3.4.23.35	alpha Factor + H2O	strict substrate specificity	Saccharomyces cerevisiae	Trp-His-Trp-Leu-Gln-Leu + Lys-Pro-Gly-Gln-Pro-Met-Tyr	-	?	16501	
3.4.23.35	alpha Factor + H2O	i.e. Trp-His-Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln-Pro-Met-Tyr, a Saccharomyces cerevisiae peptide mating pheromone secreted by mating-type alpha cells	Saccharomyces cerevisiae	Trp-His-Trp-Leu-Gln-Leu + Lys-Pro-Gly-Gln-Pro-Met-Tyr	-	?	16501	
3.4.23.35	alpha Factor + H2O	i.e. Trp-His-Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln-Pro-Met-Tyr, involved in physiological changes preceding Saccharomyces cerevisiae cell fusion	Saccharomyces cerevisiae	?	-	?	369550	
3.4.23.35	alpha Factor + H2O	acts as antagonist of alpha factor, the peptide mating pheromone produced by mating type alpha-cells	Saccharomyces cerevisiae	?	-	?	369550	
3.4.23.35	His-Trp-Leu-Gln-Leu-Arg-Pro-Gly-Gln-Pro-Met-Tyr + H2O	cleavage site: Leu-Arg	Saccharomyces cerevisiae	His-Trp-Leu-Gln-Leu + Arg-Pro-Gly-Gln-Pro-Met-Tyr	-	?	16504	
3.4.23.35	His-Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln-Pro-Met-Tyr + H2O	cleavage site: Leu-Lys	Saccharomyces cerevisiae	His-Trp-Leu-Gln-Leu + Lys-Pro-Gly-Gln-Pro-Met-Tyr	-	?	16502	
3.4.23.35	additional information	substrate specificity	Saccharomyces cerevisiae	?	-	?	89	
3.4.23.35	additional information	requires at least 4 amino acid residues on N-terminal and more than 4 on C-terminal side	Saccharomyces cerevisiae	?	-	?	89	
3.4.23.35	additional information	no hydrolysis of alpha factor derived peptides Leu-Gln-Leu-Lys-Pro-Gly-Gln-Pro-Met-Tyr, Gln-Leu-Lys-Pro-Gly-Gln-Pro-Met-Tyr, His-Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln, Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln, Trp-Leu-Gln-Leu-Lys-Pro-Gly	Saccharomyces cerevisiae	?	-	?	89	
3.4.23.35	additional information	Trp-Leu-Gln-Leu-Lys-Pro	Saccharomyces cerevisiae	?	-	?	89	
3.4.23.35	additional information	Leu-Gln-Leu-Lys, Leu-Gln-Leu-Lys-Pro, Trp-Leu-Gln-Leu, His-Trp-Leu-Gln-Leu, Trp-Leu-Gln-Leu-Lys, Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln-Pro, Trp-His-Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln-Pro-lactone, Saccharomyces kluyveri alpha factor, tosyl-L-Lys chloromethylketone, tosyl-L-Arg methyl ester, 1,2-epoxy-3-(4-nitrophenoxy)propane, diazoacetyl-DL-norleucine ester, pepstatin	Saccharomyces cerevisiae	?	-	?	89	
3.4.23.35	additional information	the reduced and alkylated B-chain of platelet-derived growth factor, synthetic peptides Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu, Ala-Pro-Ala-Lys-Phe-(4-nitro)Phe-Arg-Leu, Arg-Phe-Leu-Glu-(4-nitro)Phe-Lys-Pro-Gly-Gln-Pro, Lys-Pro-Glu-Ile-(4-nitro)Phe-Lys-Ser-Glu-Lys-Ile	Saccharomyces cerevisiae	?	-	?	89	
3.4.23.35	Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln-Pro-Met-Tyr + H2O	cleavage site: Leu-Lys	Saccharomyces cerevisiae	Trp-Leu-Gln-Leu + Lys-Pro-Gly-Gln-Pro-Met-Tyr	-	?	16503	
3.4.23.35	WHWLQLKQGQPMY + H2O	absolutely specific for the single neutral substrate peptide pheromone alpha-factor	Saccharomyces cerevisiae	WHWLQL + KQGQPMY	-	?	383409	
3.4.23.35	WHWLQLKQGQPMY + H2O	absolutely specific for the single neutral substrate peptide pheromone alpha-factor, commercial substrate preparation	Saccharomyces cerevisiae	WHWLQL + KQGQPMY	-	?	383409	
3.4.23.35	WHWLQLKQGQPMY + H2O	absolutely specific for the single neutral substrate peptide pheromone alpha-factor	Saccharomyces cerevisiae X2180-1A	WHWLQL + KQGQPMY	-	?	383409	
3.4.23.35	WHWLQLKQGQPMY + H2O	absolutely specific for the single neutral substrate peptide pheromone alpha-factor, commercial substrate preparation	Saccharomyces cerevisiae X2180-1A	WHWLQL + KQGQPMY	-	?	383409