3.4.23.21 Abz-LSFMAIQ-EDDnp + H2O - Rhizomucor miehei Abz-LSF + MAIQ-EDDnp - ? 449454 3.4.23.21 Ac-Ala-Ala-(4-nitro)Phe-Ala-Ala + H2O - Rhizopus sp. ? - ? 16314 3.4.23.21 Ac-Ala-Ala-Lys-(4-nitro)Phe-Ala-Ala + H2O - Rhizopus sp. Ac-Ala-Ala-Lys + (4-nitro)Phe-Ala-Ala - ? 453129 3.4.23.21 casein + H2O - Rhizopus arrhizus ? - ? 15445 3.4.23.21 casein + H2O - Rhizopus niveus ? - ? 15445 3.4.23.21 casein + H2O - Rhizomucor miehei ? - ? 15445 3.4.23.21 FVNQHLCGSHLVEALYLVCGERGFFYTPKA + H2O i.e. insulin B chain, cleavage site specificity Rhizopus arrhizus L-Phe + VNQH + LCGSH + L-Leu + L-Val + L-Glu + L-Ala + L-Leu + L-Tyr + LVCG + ERG + L-Phe + L-Phe + YTPKA - ? 384742 3.4.23.21 FVNQHLCGSHLVEALYLVCGERGFFYTPKA + H2O i.e. insulin B chain, cleavage site specificity Rhizopus microsporus var. chinensis L-Phe + VNQH + LCGSH + L-Leu + L-Val + L-Glu + L-Ala + L-Leu + L-Tyr + LVCG + ERG + L-Phe + L-Phe + YTPKA - ? 384742 3.4.23.21 KAIEF p-nitrophenylalanine-RL + H2O - Rhizopus sp. KAIEF + p-nitrophenylalanine-RL - ? 366942 3.4.23.21 KLIEF p-nitrophenylalanine-RL + H2O - Rhizopus sp. KLIEF + p-nitrophenylalanine-RL - ? 366941 3.4.23.21 KPAEF p-nitrophenylalanine-RL + H2O - Rhizopus sp. KPAEF + p-nitrophenylalanine-RL - ? 366944 3.4.23.21 KPALF p-nitrophenylalanine-RL + H2O - Rhizopus sp. KPALF + p-nitrophenylalanine-RL - ? 366953 3.4.23.21 KPDEF p-nitrophenylalanine-RL + H2O - Rhizopus sp. KPDEF + p-nitrophenylalanine-RL - ? 366947 3.4.23.21 KPIAF p-nitrophenylalanine-RL + H2O - Rhizopus sp. KPIAF + p-nitrophenylalanine-RL - ? 366949 3.4.23.21 KPIDF p-nitrophenylalanine-RL + H2O - Rhizopus sp. KPIDF + p-nitrophenylalanine-RL - ? 366952 3.4.23.21 KPIEF p-nitrophenylalanine-RL + H2O - Rhizopus sp. KPIEF + p-nitrophenylalanine-RL - ? 366940 3.4.23.21 KPILF p-nitrophenylalanine-RL + H2O - Rhizopus sp. KPILF + p-nitrophenylalanine-RL - ? 366951 3.4.23.21 KPISF p-nitrophenylalanine-RL + H2O - Rhizopus sp. KPISF + p-nitrophenylalanine-RL - ? 366950 3.4.23.21 KPLEF p-nitrophenylalanine-RL + H2O - Rhizopus sp. KPLEF + p-nitrophenylalanine-RL - ? 366945 3.4.23.21 KPREF p-nitrophenylalanine-RL + H2O - Rhizopus sp. KPREF + p-nitrophenylalanine-RL - ? 366948 3.4.23.21 KPRRPYILKRGSYYY + H2O synthetic neurotensin-like peptide, cleavage site specificity Rhizopus microsporus var. chinensis KPRRPYIL + KRGSYYY - ? 384955 3.4.23.21 KPSEF p-nitrophenylalanine-RL + H2O - Rhizopus sp. KPSEF + p-nitrophenylalanine-RL - ? 366946 3.4.23.21 KPVSY(4-NO2-F)RL + H2O - Rhizopus microsporus var. chinensis ? - ? 384956 3.4.23.21 KPVSY(NO2)FRL + H2O - Rhizopus sp. ? - ? 366937 3.4.23.21 KSIEF p-nitrophenylalanine-RL + H2O - Rhizopus sp. KSIEF + p-nitrophenylalanine-RL - ? 366943 3.4.23.21 L-Orn-L-Leu-D-Phe-L-Pro-L-Val-OH + H2O open chain of gramicidin S that is prepared by the treatment of gramicidin S with Bacillus subtilis alkaline protease Rhizopus microsporus var. chinensis L-Orn + L-Val + L-Leu-D-Phe-L-Pro-OH - ? 16313 3.4.23.21 Lys-Pro-Ala-Lys-Phe-(NO2)Phe-Arg-Leu + H2O - Rhizopus sp. Lys-Pro-Ala-Lys-Phe + (NO2)Phe-Arg-Leu - ? 366938 3.4.23.21 Lys-Pro-Ile-Glu-Phe-(NO2)Phe-Arg-Leu + H2O - Rhizopus sp. Lys-Pro-Ile-Glu-Phe + (NO2)Phe-Arg-Leu - ? 366939 3.4.23.21 milk powder + H2O - Rhizomucor miehei ? - ? 450291 3.4.23.21 additional information milk clotting activity Rhizopus arrhizus ? - ? 89 3.4.23.21 additional information milk clotting activity Rhizopus microsporus var. chinensis ? - ? 89 3.4.23.21 additional information peptide bonds susceptible to the action of the enzyme posess mainly bulky amino acids Rhizopus microsporus var. chinensis ? - ? 89 3.4.23.21 additional information trypsinogen activation (at pH 3.4) Rhizopus microsporus var. chinensis ? - ? 89 3.4.23.21 additional information trypsinogen activation Rhizopus arrhizus ? - ? 89 3.4.23.21 additional information trypsinogen activation Rhizopus microsporus var. chinensis ? - ? 89 3.4.23.21 additional information the enzyme shows milk clotting activity with skim milk as substrate Rhizopus arrhizus ? - ? 89 3.4.23.21 additional information very broad substrate specificity, overview Rhizopus arrhizus ? - ? 89 3.4.23.21 additional information very broad substrate specificity, overview Rhizopus microsporus var. chinensis ? - ? 89 3.4.23.21 additional information very broad substrate specificity, overview Rhizopus niveus ? - ? 89 3.4.23.21 additional information the coagulant activity of the peptidase is higher than the proteolytic activity and there is a preference for aromatic, basic, and nonpolar amino acids, particularly methionine, with specific cleavage of the peptide bond between phenylalanine and methionine Rhizomucor miehei ? - ? 89 3.4.23.21 Na-Polyglutamate + H2O - Rhizopus microsporus var. chinensis Glu + Glu-Glu + unknown compound - ? 16311 3.4.23.21 oxidized insulin B chain + H2O - Rhizopus sp. ? - ? 37092 3.4.23.21 Oxidized insulin B-chain + H2O - Rhizopus niveus Proteolytically cleaved oxidized insulin B-chain - ? 16309 3.4.23.21 Oxidized insulin B-chain + H2O splits at twelve sites, preferentially Leu-Val, Tyr-Leu and Phe-Phe Rhizopus microsporus var. chinensis Proteolytically cleaved oxidized insulin B-chain - ? 16309 3.4.23.21 Oxidized insulin B-chain + H2O primarily the Leu15-Tyr16 bond and the Tyr16-Leu17 bond is hydrolyzed, additional cleavage of the bonds Ala14-Leu15 and Phe24-Phe25 Rhizopus arrhizus Proteolytically cleaved oxidized insulin B-chain - ? 16309 3.4.23.21 Oxidized insulin B-chain + H2O bovine insulin Rhizopus microsporus var. chinensis Proteolytically cleaved oxidized insulin B-chain - ? 16309 3.4.23.21 Peptides + H2O obtained from oxidized B-chain of insulin Rhizopus niveus ? - ? 16310 3.4.23.21 Peptides + H2O in these examples the term -+- depicts the points of cleavage: Lys-+-Tyr-+-Glu-OH, benzyloxycarbonyl-Glu-+-Tyr-OH, benzyloxycarbonyl-Phe-+-Tyr-OH, benzyloxycarbonyl-Leu-+-Tyr-OH, Gly-Glu-+-Tyr-OH, benzyloxycarbonyl-Glu-+-Tyr-OH, Leu-Tyr-OH, Glu-Tyr-OH, benzyloxycarbonyl-Gly-+-Phe-OH, benzyloxycarbonyl-Glu-+-Phe-OH, Leu-Gly-+-Phe-OHGlu-Gly-+-Phe-OH, Leu-Phe-OH Rhizopus microsporus var. chinensis ? - ? 16310 3.4.23.21 Peptides + H2O specificity towards the tetradecapeptide: Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr-Ser Rhizopus sp. ? - ? 16310 3.4.23.21 Peptides + H2O role of the Asp77 in facilitating the cleavage of oligopeptide substrates with lysine in P1 Rhizopus sp. ? - ? 16310 3.4.23.21 Polylysine-HCl + H2O - Rhizopus microsporus var. chinensis Lys-Lys + Lys-Lys-Lys + higher oligolysine - ? 16312 3.4.23.21 Z-Ala-Ala-Lys-Ala-Ala-Ala + H2O - Rhizopus microsporus var. chinensis Z-Ala-Ala-Lys + Ala-Ala-Ala - ? 385946