3.4.22.B75	(di-SUMO-2)-Ran GTPase-activating protein 1 conjugate + H2O	SENP7 displays isopeptidase activity against di-SUMO-2- and SUMO-2-modified Ran GTPase-activating protein 1 but has limited activity against SUMO-1-modified Ran GTPase-activating protein 1	Homo sapiens	?	-	?	415914	
3.4.22.B75	(K335, K372, K382)-triSUMOyl-cyclic GMP-AMP synthase + 3 H2O	SUMO is conjugated onto the lysine residues 335, 372 and 382. SENP7 reverses this inhibition via catalyzing the deSUMOylation	Homo sapiens	3 SUMO + cyclic GMP-AMP synthase	-	?	449115	
3.4.22.B75	(SUMO-2)-Ran GTPase-activating protein 1 conjugate + H2O	SENP7 displays isopeptidase activity against di-SUMO-2- and SUMO-2-modified Ran GTPase-activating protein 1 but has limited activity against SUMO-1-modified Ran GTPase-activating protein 1	Homo sapiens	?	-	?	415965	
3.4.22.B75	(SUMO-3)-Ran GTPase-activating protein 1 conjugate + H2O	SENP6 and SENP7 prefer SUMO2 or SUMO3 in deconjugation reactions with rates comparable with those catalyzed by SENP2. In contrast to SENP2, SENP6 and SENP7 are less able to deconjugate SUMO1-RanGAP1, and products are only detected at enzyme concentrations 100 x higher than that observed for reactions containing SENP2	Homo sapiens	SUMO-3 + Ran GTPase-activating protein 1	-	?	415971	
3.4.22.B75	acetyl-LRGG-7-amido-4-trifluoromethylcoumarin + H2O	SENP7 prefers the LRGG sequence, residues typical of Nedd8 or ubiquitin in the P3 and P4 positions	Homo sapiens	acetyl-LRGG + 7-amino-4-trifluoromethylcoumarin	-	?	416497	
3.4.22.B75	acetyl-QTGG-7-amido-4-trifluoromethylcoumarin + H2O	very low activity	Homo sapiens	acetyl-QTGG + 7-amino-4-trifluoromethylcoumarin	-	?	416498	
3.4.22.B75	axin1 + H2O	-	Homo sapiens	?	-	?	449625	
3.4.22.B75	di-SUMO-2 + H2O	-	Homo sapiens	2 SUMO-2	-	?	416897	
3.4.22.B75	di-SUMO-3 + H2O	-	Homo sapiens	2 SUMO-3	-	?	416898	
3.4.22.B75	additional information	SENP7 has a restricted substrate specificity, being unable to process SUMO precursors and displaying paralogue-specific isopeptidase activity	Homo sapiens	?	-	?	89	
3.4.22.B75	additional information	SENP7 reveals no detectable endopeptidase activity on the precursors of ubiquitin, Nedd8 and ISG15	Homo sapiens	?	-	?	89	
3.4.22.B75	additional information	a module of two PxVxL HP1 interaction motifs on enzyme SENP7 mediates association with HP1, molecular basis for the interaction between SENP7 and HP1, the capacity for SENP7 to interact with HP1 in cells and its capacity to de-SUMOylate SUMO1-HP1 are independent	Mus musculus	?	-	?	89	
3.4.22.B75	additional information	the enzyme SENP7 interacts with the chromatin repressive KRAB-associated protein 1 through heterochromatin protein 1 alpha, HP1alapha. Enzyme SENP7 contains a conserved HP1-box, PxVxL, required for interaction with HP1	Homo sapiens	?	-	?	89	
3.4.22.B75	additional information	isozyme SENP7 shows isoform specificity for SUMO2/3 over SUMO1	Homo sapiens	?	-	?	89	
3.4.22.B75	additional information	long enzyme variant SENP7L, but not short enzyme variant SENP7S, binds HP1alpha. This interaction does not require catalytic activity: both the active wild-type SENP7L and the catalytically inactive mutant (SENP7Lm) bind endogenous HP1alpha efficiently	Homo sapiens	?	-	?	89	
3.4.22.B75	additional information	SENP7 is a SUMO protease with a preference for deconjugating SUMO2 and SUMO3, particularly in a polymeric form. Precursor SUMO1, SUMO2 and SUMO3 and SUMO1 polymers are poorly processed by SENP7	Homo sapiens	?	-	?	89	
3.4.22.B75	poly-SUMO-2 + H2O	-	Homo sapiens	?	-	?	415722	
3.4.22.B75	poly-SUMO-2 + H2O	the C-terminal catalytic domain of SENP7 efficiently depolymerized poly-SUMO-2 chains but has undetectable activity against poly-SUMO-1 chains	Homo sapiens	?	-	?	415722	
3.4.22.B75	poly-SUMO-3 + H2O	-	Homo sapiens	?	-	?	417503	
3.4.22.B75	polySUMO2 KRAB-associated protein 1 + H2O	enzyme SENP7 promotes the removal of SUMO2 from KRAB-associated protein 1	Homo sapiens	KRAB-associated protein 1 + SUMO 2 + SUMO3	-	?	432305	
3.4.22.B75	SUMO-1 precursor + H2O	SENP7 exhibits lower rates for processing pre-SUMO-1, pre-SUMO-2, or pre-SUMO-3 in comparison with SENP2	Homo sapiens	SUMO-1 + His-Ser-Thr-Val	-	?	415762	
3.4.22.B75	SUMO-2 precursor + H2O	SENP7 exhibits lower rates for processing pre-SUMO1, pre-SUMO-2, or pre-SUMO-3 in comparison with SENP2	Homo sapiens	SUMO-2 + Val-Tyr	-	?	415763	
3.4.22.B75	SUMO-3 precursor + H2O	SENP7 exhibits lower rates for processing pre-SUMO1, pre-SUMO-2, or pre-SUMO-3 in comparison with SENP2	Homo sapiens	SUMO-3 + Val-Pro-Glu-Ser-Ser-Leu-Ala-Gly-His-Ser-Phe	-	?	415764	
3.4.22.B75	SUMO1-HP1 + H2O	deSUMOylation	Mus musculus	HP1 + SUMO1	-	?	431202	
3.4.22.B75	SUMO2/3ylated HP1alpha + H2O	HP1alpha localizes at the pericentric heterochromatin, importance of SUMOylation in directing HP1alpha’s subnuclear localization, SUMO deconjugation by enzyme SENP7	Homo sapiens	HP1alpha + SUMO3 + SUMO2	-	?	431204	
3.4.22.B75	SUMO2/3ylated HP1alpha + H2O	SUMO deconjugation by enzyme SENP7	Homo sapiens	HP1alpha + SUMO3 + SUMO2	-	?	431204	
3.4.22.B75	SUMOylated beta-catenin + H2O	-	Homo sapiens	?	-	?	450710	
3.4.22.B75	SUMOylated c-Myc + H2O	-	Homo sapiens	deSUMOylated c-Myc + SUMO	-	?	431207	
3.4.22.B75	SUMOylated HP1alpha + H2O	SUMO deconjugation by enzyme SENP7	Mus musculus	HP1alpha + SUMO	-	?	431211	
3.4.22.B75	SUMOylated KRAB-associated protein 1 + H2O	enzyme SENP7 promotes the removal of SUMO2/3 from KRAB-associated protein 1	Homo sapiens	KRAB-associated protein 1 + SUMO 2 + SUMO3	-	?	431212