3.4.22.B14 4-[[4-(dimethylamino)phenyl]azo]benzoic acid-MYNKMGGGDKTVSF-(5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid) amide + H2O - Transmissible gastroenteritis virus ? - ? 411387 3.4.22.B14 4-[[4-(dimethylamino)phenyl]azo]benzoic acid-MYNKMGGGDKTVSF-(5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid) amide + H2O - Transmissible gastroenteritis virus Purdue ? - ? 411387 3.4.22.B14 coronavirus nonstructural protein 1 + H2O - Murine hepatitis virus ? - ? 411897 3.4.22.B14 coronavirus nonstructural protein 2 + H2O - Murine hepatitis virus ? - ? 411898 3.4.22.B14 coronavirus nonstructural protein 3 + H2O - Murine hepatitis virus ? - ? 411899 3.4.22.B14 ISG15-7-amido-4-methylcoumarin + H2O - Severe acute respiratory syndrome-related coronavirus ISG15 + 7-amino-4-methylcoumarin - ? 450087 3.4.22.B14 ISG15-7-amido-4-methylcoumarin + H2O - Middle East respiratory syndrome-related coronavirus ISG15 + 7-amino-4-methylcoumarin - ? 450087 3.4.22.B14 KAGG-7-amido-4-methylcoumarin + H2O - infectious bronchitis virus KAGG + 7-amino-4-methylcoumarin - ? 450099 3.4.22.B14 KAGG-7-amido-4-methylcoumarin + H2O - infectious bronchitis virus Beaudette KAGG + 7-amino-4-methylcoumarin - ? 450099 3.4.22.B14 KKAG-7-amido-4-methylcoumarin + H2O - infectious bronchitis virus KKAG + 7-amino-4-methylcoumarin - ? 450107 3.4.22.B14 KKAG-7-amido-4-methylcoumarin + H2O - infectious bronchitis virus Beaudette KKAG + 7-amino-4-methylcoumarin - ? 450107 3.4.22.B14 L-Arg-L-Leu-L-Arg-Gly-Gly-7-amido-4-methylcoumarin + H2O - Severe acute respiratory syndrome-related coronavirus L-Arg-L-Leu-L-Arg-Gly-Gly + 7-amino-4-methylcoumarin - ? 412228 3.4.22.B14 LRGG-7-amido-4-methylcoumarin + H2O - infectious bronchitis virus LRGG + 7-amino-4-methylcoumarin - ? 450237 3.4.22.B14 LRGG-7-amido-4-methylcoumarin + H2O - infectious bronchitis virus Beaudette LRGG + 7-amino-4-methylcoumarin - ? 450237 3.4.22.B14 Lys48-Ub4 + H2O - infectious bronchitis virus ? - ? 450250 3.4.22.B14 Lys48-Ub4 + H2O - infectious bronchitis virus Beaudette ? - ? 450250 3.4.22.B14 Lys63-Ub4 + H2O - infectious bronchitis virus ? - ? 450251 3.4.22.B14 additional information viral substrates translated from pSPDELTAMscN1S1, p28, p43 and p70, efficient cleavage requires a substrate greater than 69000 Da Murine coronavirus ? - ? 89 3.4.22.B14 additional information the virus can regulate in vivo proteinase activity through self inactivation of PLP-1. The lack of p50 production in cells infected with the mouse hepatitis virus strain JHM may regulate PLP-1 activity differently from MHV-59 Murine coronavirus ? - ? 89 3.4.22.B14 additional information interactions of PLP1 with CS1 and CS2 are critical for protein processing and suggest that the interactions play specific roles in regulation of the functions of nsp1, 2, and 3 in viral RNA synthesis Murine hepatitis virus ? - ? 89 3.4.22.B14 additional information PL1pro cleaves a peptide mimicking the cognate nsp2-nsp3 cleavage site, has deubiquitinating activity in an in vitro cleavage assay and displays a slight preference for Lys48-linked polyubiquitin chains over Lys63-linked ones Transmissible gastroenteritis virus ? - ? 89 3.4.22.B14 additional information enzyme is able to cleave ubiquitin mutant L73P in K6-, K11-, K48- and K63-linked di-ubiquitin chains. Cleavage rates with MERS PLpro are nearly identical against ubiquitin chains of various lengths Middle East respiratory syndrome-related coronavirus ? - ? 89 3.4.22.B14 additional information MERS PLpro has an absolute requirement for Gly in the P2 position, but can accommodate a wide variety of hydrophobic amino acids in both the P3 and P4 positions. The most preferred amino acid in P3 is Leu, followed by Arg, Lys and hydrophobic residues. The most preferred amino acid in P4 is also Leu, followed by mostly hydrophobic residues Middle East respiratory syndrome-related coronavirus ? - ? 89 3.4.22.B14 additional information PLpro can degrade Lys48- and Lys63-linked polyubiquitin chains to monoubiquitin but not linear polyubiquitin infectious bronchitis virus ? - ? 89 3.4.22.B14 additional information SARS PLpro recognizes a K48-linked tri-Ub as a minimal unit for ubiquitin chain processing Severe acute respiratory syndrome-related coronavirus ? - ? 89 3.4.22.B14 additional information the virus can regulate in vivo proteinase activity through self inactivation of PLP-1. The lack of p50 production in cells infected with the mouse hepatitis virus strain JHM may regulate PLP-1 activity differently from MHV-59 Murine coronavirus JHM ? - ? 89 3.4.22.B14 additional information viral substrates translated from pSPDELTAMscN1S1, p28, p43 and p70, efficient cleavage requires a substrate greater than 69000 Da Murine coronavirus JHM ? - ? 89 3.4.22.B14 additional information PL1pro cleaves a peptide mimicking the cognate nsp2-nsp3 cleavage site, has deubiquitinating activity in an in vitro cleavage assay and displays a slight preference for Lys48-linked polyubiquitin chains over Lys63-linked ones Transmissible gastroenteritis virus Purdue ? - ? 89 3.4.22.B14 additional information PLpro can degrade Lys48- and Lys63-linked polyubiquitin chains to monoubiquitin but not linear polyubiquitin infectious bronchitis virus Beaudette ? - ? 89 3.4.22.B14 ORF 1a polyprotein + H2O - Transmissible gastroenteritis virus ? - ? 361958 3.4.22.B14 ORF 1a polyprotein + H2O - Murine coronavirus ? - ? 361958 3.4.22.B14 ORF 1a polyprotein + H2O cleavage in cis and in trans Murine coronavirus ? - ? 361958 3.4.22.B14 ORF 1a polyprotein + H2O cleavage sites are: Gly247-/-Val248 and Gly821-/-Val822. PLP-1 is responsible for the cleavage of the amino-terminal protein p28 Murine coronavirus ? - ? 361958 3.4.22.B14 ORF 1a polyprotein + H2O cleavage in cis and in trans. PLP-1 cleavage activity appears to be optimal when both enzyme and substrate are present as part of polyproteins, as they are in the virus replication cycle Murine coronavirus ? - ? 361958 3.4.22.B14 ORF 1a polyprotein + H2O the enzyme is involved in processing of ORF 1a polyprotein into mature replicase-related polypeptides Murine coronavirus ? - ? 361958 3.4.22.B14 ORF 1a polyprotein + H2O - Murine coronavirus JHM ? - ? 361958 3.4.22.B14 ORF 1a polyprotein + H2O cleavage in cis and in trans Murine coronavirus JHM ? - ? 361958 3.4.22.B14 ORF 1a polyprotein + H2O cleavage in cis and in trans. PLP-1 cleavage activity appears to be optimal when both enzyme and substrate are present as part of polyproteins, as they are in the virus replication cycle Murine coronavirus JHM ? - ? 361958 3.4.22.B14 ORF 1a polyprotein + H2O cleavage sites are: Gly247-/-Val248 and Gly821-/-Val822. PLP-1 is responsible for the cleavage of the amino-terminal protein p28 Murine coronavirus JHM ? - ? 361958 3.4.22.B14 ORF 1a polyprotein + H2O the enzyme is involved in processing of ORF 1a polyprotein into mature replicase-related polypeptides Murine coronavirus JHM ? - ? 361958 3.4.22.B14 ORF 1a polyprotein + H2O - Transmissible gastroenteritis virus Purdue ? - ? 361958 3.4.22.B14 ORF 1a polyprotein + H2O SARS coronavirus replicase polyproteins pp1a and pp1ab are predicted to be processed into 16 nonstructural proteins, NSP2-3 intermediate identified Severe acute respiratory syndrome-related coronavirus nsp1 + nsp2 + nsp3 identified products ? 385476 3.4.22.B14 ORF 1a polyprotein + H2O SARS coronavirus replicase polyproteins pp1a and pp1ab are predicted to be processed into 16 nonstructural proteins, NSP2-3 intermediate identified Severe acute respiratory syndrome-related coronavirus Urbani nsp1 + nsp2 + nsp3 identified products ? 385476 3.4.22.B14 p28 - Murine hepatitis virus ? - ? 383277 3.4.22.B14 p65 - Murine hepatitis virus ? - ? 383278 3.4.22.B14 proISG + H2O i.e. proenzyme of interferon-stimulated gene product 15 Severe acute respiratory syndrome-related coronavirus ? - ? 450512 3.4.22.B14 tetra-ubiquitin + H2O - Severe acute respiratory syndrome-related coronavirus 2 di-ubiquitin - ? 450749 3.4.22.B14 ubiquitin-7-amido-4-methylcoumarin + H2O - Transmissible gastroenteritis virus ubiquitin + 7-amino-4-methylcoumarin - ? 360721 3.4.22.B14 ubiquitin-7-amido-4-methylcoumarin + H2O - Severe acute respiratory syndrome-related coronavirus ubiquitin + 7-amino-4-methylcoumarin - ? 360721 3.4.22.B14 ubiquitin-7-amido-4-methylcoumarin + H2O - Middle East respiratory syndrome-related coronavirus ubiquitin + 7-amino-4-methylcoumarin - ? 360721 3.4.22.B14 ubiquitin-7-amido-4-methylcoumarin + H2O - Transmissible gastroenteritis virus Purdue ubiquitin + 7-amino-4-methylcoumarin - ? 360721 3.4.22.B14 ubiquitinated RIG-I + H2O substrate is a key fegulator in the IFN signaling pathway Transmissible gastroenteritis virus ubiquitin + RIG-I - ? 450792 3.4.22.B14 ubiquitinated STING + H2O substrate is a key fegulator in the IFN signaling pathway Transmissible gastroenteritis virus ubiquitin + STINGI - ? 450793