3.4.22.7 azocasein + H2O - Asclepias curassavica ? - ? 15347 3.4.22.7 benzoyl-L-Arg-p-nitroanilide + H2O - Asclepias syriaca benzoyl-L-Arg + 4-nitroaniline - ? 65319 3.4.22.7 casein + H2O - Asclepias syriaca ? - ? 15445 3.4.22.7 casein + H2O - Asclepias glaucescens ? - ? 15445 3.4.22.7 casein + H2O - Asclepias curassavica ? - ? 15445 3.4.22.7 casein + H2O about 50% degradation after 6 h Gomphocarpus fruticosus ? - ? 15445 3.4.22.7 casein + H2O - Asclepias glaucescens H.B.K ? - ? 15445 3.4.22.7 L-pyroglutamyl-L-phenylalanyl-L-leucine 4-nitroanilide + H2O very low activity Asclepias curassavica L-pyroglutamyl-L-phenylalanyl-L-leucine + 4-nitroaniline - ? 412331 3.4.22.7 L-pyroglutamyl-L-phenylalanyl-L-leucine-4-nitroanilide + H2O - Asclepias curassavica ? - ? 385057 3.4.22.7 L-pyroglutamyl-Phe-Leu-p-nitroanilide + H2O - Asclepias curassavica L-pyroglutamyl-Phe-Leu + p-nitroaniline - ? 412332 3.4.22.7 additional information asclepain cI is the main active enzyme, but shows lower specific activity than asclepain cII, which is the minor proteolytic component in the latex, overview Asclepias curassavica ? - ? 89 3.4.22.7 additional information asclepain cII is the minor proteolytic component in the latex, but shows higher specific activity than asclepain cI, the main active enzyme, overview Asclepias curassavica ? - ? 89 3.4.22.7 additional information the peptide PFLNA is a poor substrate for asclepain cII Asclepias curassavica ? - ? 89 3.4.22.7 additional information no activity towards N-alpha-benzyloxycarbonyl-L-Val-4-nitrophenyl ester, N-alpha-benzyloxycarbonyl-L-Leu-4-nitrophenyl ester, N-alpha-benzyloxycarbonyl-L-Ile-4-nitrophenyl ester, and N-alpha-benzyloxycarbonyl-L-Lys-4-nitrophenyl ester Asclepias curassavica ? - ? 89 3.4.22.7 additional information ascpelain f shows low milk clotting activity Gomphocarpus fruticosus ? - ? 89 3.4.22.7 additional information no activity towards Nalpha-carbobenzoxy-L-Leu-4-nitrophenyl ester, Nalpha-carbobenzoxy-L-Asn-4-nitrophenyl ester, Nalpha-carbobenzoxy-L-Trp-4-nitrophenyl ester, Nalpha-carbobenzoxy-L-Val-4-nitrophenyl ester, Nalpha-carbobenzoxy-L-Asp-4-nitrophenyl ester, Nalpha-carbobenzoxy-L-lys-4-nitrophenyl ester, Nalpha-carbobenzoxy-L-Phe-4-nitrophenyl ester, Nalpha-carbobenzoxy-L-Pro-4-nitrophenyl ester, and Nalpha-carbobenzoxy-L-Ile-4-nitrophenyl ester Gomphocarpus fruticosus ? - ? 89 3.4.22.7 additional information the cut specificity is governed by the presence of hydrophobic residues (F, L, V) in the P2 position Gomphocarpus fruticosus subsp. fruticosus ? - ? 89 3.4.22.7 N-acetyl-L-tryptophanamide + H2O - Asclepias glaucescens N-acetyl-L-Trp + NH3 - ? 65321 3.4.22.7 N-acetyl-L-tryptophanamide + H2O - Asclepias glaucescens H.B.K N-acetyl-L-Trp + NH3 - ? 65321 3.4.22.7 N-alpha-benzyloxycarbonyl-Gly-4-nitrophenyl ester + H2O - Asclepias curassavica N-alpha-benzyloxycarbonyl-Gly + 4-nitrophenol - ? 412498 3.4.22.7 N-alpha-benzyloxycarbonyl-L-Ala-4-nitrophenyl ester + H2O - Asclepias curassavica N-alpha-benzyloxycarbonyl-L-Ala + 4-nitrophenol - ? 412499 3.4.22.7 N-alpha-benzyloxycarbonyl-L-Asn-4-nitrophenyl ester + H2O - Asclepias curassavica N-alpha-benzyloxycarbonyl-L-Asn + 4-nitrophenol - ? 412500 3.4.22.7 N-alpha-benzyloxycarbonyl-L-Asp-4-nitrophenyl ester + H2O asclepain cII has highest endoesterolytic activity using N-alpha-benzyloxycarbonyl-amino acid p-nitrophenyl esters with L-Asp as amino acid Asclepias curassavica N-alpha-benzyloxycarbonyl-L-Asp + 4-nitrophenol - ? 412501 3.4.22.7 N-alpha-benzyloxycarbonyl-L-Gln-4-nitrophenyl ester + H2O - Asclepias curassavica N-alpha-benzyloxycarbonyl-L-Gln + 4-nitrophenol - ? 412502 3.4.22.7 N-alpha-benzyloxycarbonyl-L-Tyr-4-nitrophenyl ester + H2O - Asclepias curassavica N-alpha-benzyloxycarbonyl-L-Tyr + 4-nitrophenol - ? 412503 3.4.22.7 N-Benzoyl-L-Arg ethyl ester + H2O - Asclepias glaucescens N-Benzoyl-L-Arg + ethanol - ? 15729 3.4.22.7 N-Benzoyl-L-Arg ethyl ester + H2O - Asclepias glaucescens H.B.K N-Benzoyl-L-Arg + ethanol - ? 15729 3.4.22.7 N-carbobenzoxy-Gly p-nitrophenyl ester + H2O - Asclepias syriaca N-carbobenzoxy-Gly + p-nitrophenol - ? 65316 3.4.22.7 N-carbobenzoxy-L-Ala 4-nitrophenyl ester + H2O 47% of the activity with N-carbobenzoxy-L-Gln 4-nitrophenyl ester Asclepias curassavica N-carbobenzoxy-L-Ala + 4-nitrophenol - ? 385283 3.4.22.7 N-carbobenzoxy-L-Gln 4-nitrophenyl ester + H2O - Asclepias curassavica N-carbobenzoxy-L-Gln + 4-nitrophenol - ? 385286 3.4.22.7 N-carbobenzoxy-L-Ile 4-nitrophenyl ester + H2O 59% of the activity with N-carbobenzoxy-L-Gln 4-nitrophenyl ester Asclepias curassavica N-carbobenzoxy-L-Ile + 4-nitrophenol - ? 385287 3.4.22.7 N-carbobenzoxy-L-Lys 4-nitrophenyl ester + H2O 78% of the activity with N-carbobenzoxy-L-Gln 4-nitrophenyl ester Asclepias curassavica N-carbobenzoxy-L-Lys + 4-nitrophenol - ? 15732 3.4.22.7 N-carbobenzoxy-L-Tyr 4-nitrophenyl ester + H2O 51% of the activity with N-carbobenzoxy-L-Gln 4-nitrophenyl ester Asclepias curassavica N-carbobenzoxy-L-Tyr + 4-nitrophenol - ? 385288 3.4.22.7 N-carbobenzoxy-Leu p-nitrophenyl ester + H2O - Asclepias syriaca N-carbobenzoxy-Leu + p-nitrophenol - ? 65317 3.4.22.7 N-carbobenzoxy-Lys p-nitrophenyl ester + H2O - Asclepias syriaca N-carbobenzoxy-Lys + 4-nitrophenol - ? 65315 3.4.22.7 N-carbobenzoxy-Tyr p-nitrophenyl ester + H2O - Asclepias syriaca N-carbobenzoxy-Tyr + p-nitrophenol - ? 65318 3.4.22.7 N-methanesulfonylglycine 3-bromophenyl ester + H2O - Asclepias sp. N-methanesulfonylglycine + 3-bromophenol - ? 65304 3.4.22.7 N-methanesulfonylglycine 3-chlorophenyl ester + H2O - Asclepias sp. N-methanesulfonylglycine + 3-chlorophenol - ? 65306 3.4.22.7 N-methanesulfonylglycine 3-fluorophenyl ester + H2O - Asclepias sp. N-methanesulfonylglycine + 3-fluorophenol - ? 65308 3.4.22.7 N-methanesulfonylglycine 3-methylphenyl ester + H2O - Asclepias sp. N-methanesulfonylglycine + 3-methylphenol - ? 65302 3.4.22.7 N-methanesulfonylglycine 3-nitrophenyl ester + H2O - Asclepias sp. N-methanesulfonylglycine 3-nitrophenol - ? 65310 3.4.22.7 N-methanesulfonylglycine 3-tert-butylphenyl ester + H2O - Asclepias sp. N-methanesulfonylglycine + 3-tert-butylphenol - ? 65312 3.4.22.7 N-methanesulfonylglycine 4-bromophenyl ester + H2O - Asclepias sp. N-methanesulfonylglycine + 4-bromophenol - ? 65305 3.4.22.7 N-methanesulfonylglycine 4-chlorophenyl ester + H2O - Asclepias sp. N-methanesulfonylglycine + 4-chlorophenol - ? 65307 3.4.22.7 N-methanesulfonylglycine 4-fluorophenyl ester + H2O - Asclepias sp. N-methanesulfonylglycine + 4-fluorophenol - ? 65309 3.4.22.7 N-methanesulfonylglycine 4-methylphenyl ester + H2O - Asclepias sp. N-methanesulfonylglycine + 4-methylphenol - ? 65303 3.4.22.7 N-methanesulfonylglycine 4-nitrophenyl ester + H2O - Asclepias sp. N-methanesulfonylglycine + 4-nitrophenol - ? 65311 3.4.22.7 Nalpha-carbobenzoxy-Gly-4-nitrophenyl ester + H2O about 25% activity compared to Nalpha-carbobenzoxy-L-Ala-4-nitrophenyl ester Gomphocarpus fruticosus Nalpha-carbobenzoxy-Gly + 4-nitrophenol - ? 418932 3.4.22.7 Nalpha-carbobenzoxy-L-Ala-4-nitrophenyl ester + H2O 100% activity Gomphocarpus fruticosus Nalpha-carbobenzoxy-L-Ala + 4-nitrophenol - ? 418933 3.4.22.7 Nalpha-carbobenzoxy-L-Gln-4-nitrophenyl ester + H2O about 85% activity compared to Nalpha-carbobenzoxy-L-Ala-4-nitrophenyl ester Gomphocarpus fruticosus Nalpha-carbobenzoxy-L-Gln + 4-nitrophenol - ? 418935 3.4.22.7 Nalpha-carbobenzoxy-L-Tyr-4-nitrophenyl ester + H2O about 20% activity compared to Nalpha-carbobenzoxy-L-Ala-4-nitrophenyl ester Gomphocarpus fruticosus Nalpha-carbobenzoxy-L-Tyr + 4-nitrophenol - ? 418936 3.4.22.7 Nalpha-CBZ-L-Gln-4-nitrophenyl ester + H2O when assayed on N-alpha-CBZ-amino acid-4-nitrophenyl esters, the enzyme exhibits higher preference for the glutamine derivative Asclepias curassavica Nalpha-CBZ-L-Gln + 4-nitrophenol - ? 406097 3.4.22.7 oxidized insulin B chain + H2O detection of cleavage points Asclepias sp. ? - ? 37092 3.4.22.7 oxidized insulin B chain + H2O 12 cut-off points Gomphocarpus fruticosus subsp. fruticosus ? - ? 37092 3.4.22.7 pGlu-Phe-Leu 4-nitroanilide + H2O - Gomphocarpus fruticosus subsp. fruticosus pGlu-Phe-Leu + 4-nitroaniline - ? 450452 3.4.22.7 pGlu-Phe-Leu-p-nitroanilide + H2O - Gomphocarpus fruticosus subsp. fruticosus pGlu-Phe-Leu + p-nitroaniline - ? 412675 3.4.22.7 Phe-Val-Asn-Ala-His-Leu-CysA-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-CysA-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala + H2O - Asclepias syriaca Phe-Val-Asn + Ser-His-Leu-Val-Glu-Ala-Leu + Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-CysA-Gly-Glu + Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala + Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala + Gly-Phe-Phe-Tyr-Thr 14% Phe-Val-Asn + 22% Ser-His-Leu-Val-Glu-Ala-Leu + 15% Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-CysA-Gly-Glu + 10% Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala + 10% Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala + 13% Gly-Phe-Phe-Tyr-Thr ? 65313 3.4.22.7 soybean protein + H2O the enzyme is able to selectively hydrolyze soybean proteins at pH 10, employing an enzyme/substrate ratio of 0.2% (w/w). The enzymatic hydrolysis allows a strong increase in the solubility, water and oil holding capacity Gomphocarpus fruticosus subsp. fruticosus ? - ? 450634 3.4.22.7 Z-Arg-p-nitroanilide + H2O - Asclepias curassavica Z-Arg + p-nitroaniline - ? 413097