3.4.22.51 (7-[[(2S)-2-(4-[(1S)-1,2-dimethyl-1-[(morpholin-4-ylcarbonyl)amino]propyl]-1H-1,2,3-triazol-1-yl)hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O - Trypanosoma cruzi (2S)-2-(4-[(1S)-1,2-dimethyl-1-[(morpholin-4-ylcarbonyl)amino]propyl]-1H-1,2,3-triazol-1-yl)hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid - ir 403059 3.4.22.51 (7-[[(2S)-2-(4-[(1S)-1,2-dimethyl-1-[(naphthalen-2-ylcarbonyl)amino]propyl]-1H-1,2,3-triazol-1-yl)hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O - Trypanosoma cruzi (2S)-2-(4-[(1S)-1,2-dimethyl-1-[(naphthalen-2-ylcarbonyl)amino]propyl]-1H-1,2,3-triazol-1-yl)hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid - ir 403060 3.4.22.51 (7-[[(2S)-2-(4-[(1S)-1,2-dimethyl-1-[(phenylcarbonyl)amino]propyl]-1H-1,2,3-triazol-1-yl)hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O - Trypanosoma cruzi (2S)-2-(4-[(1S)-1,2-dimethyl-1-[(phenylcarbonyl)amino]propyl]-1H-1,2,3-triazol-1-yl)hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid - ir 403061 3.4.22.51 (7-[[(2S)-2-(4-[(1S)-1,2-dimethyl-1-[(quinolin-6-ylcarbonyl)amino]propyl]-1H-1,2,3-triazol-1-yl)hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O - Trypanosoma cruzi (2S)-2-(4-[(1S)-1,2-dimethyl-1-[(quinolin-6-ylcarbonyl)amino]propyl]-1H-1,2,3-triazol-1-yl)hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid - ir 403062 3.4.22.51 (7-[[(2S)-2-(4-[(1S)-1,2-dimethyl-1-[(quinolin-6-ylmethyl)amino]propyl]-1H-1,2,3-triazol-1-yl)hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O - Trypanosoma cruzi (2S)-2-(4-[(1S)-1,2-dimethyl-1-[(quinolin-6-ylmethyl)amino]propyl]-1H-1,2,3-triazol-1-yl)hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid - ir 403063 3.4.22.51 (7-[[(2S)-2-(4-[(1S)-1-[(1,3-benzothiazol-6-ylcarbonyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O - Trypanosoma cruzi (2S)-2-(4-[(1S)-1-[(1,3-benzothiazol-6-ylcarbonyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid - ir 403064 3.4.22.51 (7-[[(2S)-2-(4-[(1S)-1-[(1,3-benzothiazol-6-ylmethyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O - Trypanosoma cruzi (2S)-2-(4-[(1S)-1-[(1,3-benzothiazol-6-ylmethyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid - ir 403065 3.4.22.51 (7-[[(2S)-2-(4-[(1S)-1-[(1-benzofuran-6-ylcarbonyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O - Trypanosoma cruzi (2S)-2-(4-[(1S)-1-[(1-benzofuran-6-ylcarbonyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid - ir 403066 3.4.22.51 (7-[[(2S)-2-(4-[(1S)-1-[(1-benzothiophen-6-ylcarbonyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O - Trypanosoma cruzi (2S)-2-(4-[(1S)-1-[(1-benzothiophen-6-ylcarbonyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid - ir 403067 3.4.22.51 (7-[[(2S)-2-(4-[(1S)-1-[(1H-benzotriazol-5-ylcarbonyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O - Trypanosoma cruzi (2S)-2-(4-[(1S)-1-[(1H-benzotriazol-5-ylcarbonyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid - ir 403068 3.4.22.51 (7-[[(2S)-2-(4-[(1S)-1-[(1H-indol-5-ylcarbonyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O - Trypanosoma cruzi (2S)-2-(4-[(1S)-1-[(1H-indol-5-ylcarbonyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid - ir 403069 3.4.22.51 (7-[[(2S)-2-(4-[(1S)-1-[(1H-indol-6-ylcarbonyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O - Trypanosoma cruzi (2S)-2-(4-[(1S)-1-[(1H-indol-6-ylcarbonyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid - ir 403070 3.4.22.51 (7-[[(2S)-2-(4-[(1S)-1-[(4-methoxybenzyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O - Trypanosoma cruzi (2S)-2-(4-[(1S)-1-[(4-methoxybenzyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid - ir 403071 3.4.22.51 (7-[[(2S)-2-[4-[(1S)-1,2-dimethyl-1-[[(1-methyl-1H-indol-6-yl)carbonyl]amino]propyl]-1H-1,2,3-triazol-1-yl]hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O - Trypanosoma cruzi (2S)-2-[4-[(1S)-1,2-dimethyl-1-[[(1-methyl-1H-indol-6-yl)carbonyl]amino]propyl]-1H-1,2,3-triazol-1-yl]hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid - ir 403072 3.4.22.51 (7-[[(2S)-2-[4-[(1S)-1,2-dimethyl-1-[[(2-methylphenyl)carbonyl]amino]propyl]-1H-1,2,3-triazol-1-yl]hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O - Trypanosoma cruzi (2S)-2-[4-[(1S)-1,2-dimethyl-1-[[(2-methylphenyl)carbonyl]amino]propyl]-1H-1,2,3-triazol-1-yl]hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid - ir 403073 3.4.22.51 (7-[[(2S)-2-[4-[(1S)-1,2-dimethyl-1-[[(3-methylphenyl)carbonyl]amino]propyl]-1H-1,2,3-triazol-1-yl]hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O - Trypanosoma cruzi (2S)-2-[4-[(1S)-1,2-dimethyl-1-[[(3-methylphenyl)carbonyl]amino]propyl]-1H-1,2,3-triazol-1-yl]hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid - ir 403074 3.4.22.51 (7-[[(2S)-2-[4-[(1S)-1,2-dimethyl-1-[[(4-methylphenyl)carbonyl]amino]propyl]-1H-1,2,3-triazol-1-yl]hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O - Trypanosoma cruzi (2S)-2-[4-[(1S)-1,2-dimethyl-1-[[(4-methylphenyl)carbonyl]amino]propyl]-1H-1,2,3-triazol-1-yl]hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid - ir 403075 3.4.22.51 (7-[[(2S)-2-[4-[(1S)-1-(benzylamino)-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl]hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O - Trypanosoma cruzi (2S)-2-[4-[(1S)-1-(benzylamino)-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl]hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid - ir 403076 3.4.22.51 (7-[[(2S)-2-[4-[(1S)-1-[[(2-methoxyphenyl)carbonyl]amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl]hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O - Trypanosoma cruzi (2S)-2-[4-[(1S)-1-[[(2-methoxyphenyl)carbonyl]amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl]hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid - ir 403077 3.4.22.51 (7-[[(2S)-2-[4-[(1S)-1-[[(3-methoxyphenyl)carbonyl]amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl]hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O - Trypanosoma cruzi (2S)-2-[4-[(1S)-1-[[(3-methoxyphenyl)carbonyl]amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl]hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid - ir 403078 3.4.22.51 (7-[[(2S)-2-[4-[(1S)-1-[[(4-methoxyphenyl)carbonyl]amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl]hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O - Trypanosoma cruzi (2S)-2-[4-[(1S)-1-[[(4-methoxyphenyl)carbonyl]amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl]hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid - ir 403079 3.4.22.51 2-aminobenzoic acid-ALRFSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O - Trypanosoma cruzi ? - ? 355605 3.4.22.51 2-aminobenzoic acid-KLGFSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O - Trypanosoma cruzi ? - ? 355606 3.4.22.51 2-aminobenzoic acid-KLRFSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O and analogues Trypanosoma cruzi ? - ? 355607 3.4.22.51 2-aminobenzoic acid-peptidyl-N-(2,4-dinitrophenyl)ethylenediamine + H2O and derivatives, enzyme shows preference for benzyl-Cys or Arg at the P1 position and a hydrophobic non-aromatic residue at position P2 Trypanosoma cruzi ? - ? 355608 3.4.22.51 2-aminobenzoyl-ARF-(2,4-dinitrophenyl)-epsilon-NH2-lysine-OH + H2O - Trypanosoma cruzi ? - ? 383649 3.4.22.51 2-aminobenzoyl-FRA-(2,4-dinitrophenyl)-epsilon-NH2-lysine-NH2 + H2O - Trypanosoma cruzi ? - ? 383656 3.4.22.51 2-aminobenzoyl-FRA-(2,4-dinitrophenyl)-epsilon-NH2-lysine-OH + H2O - Trypanosoma cruzi ? - ? 383657 3.4.22.51 2-aminobenzoyl-FRF-(2,4-dinitrophenyl)-epsilon-NH2-lysine-OH + H2O - Trypanosoma cruzi ? - ? 383658 3.4.22.51 2-aminobenzoyl-LGMISLMKRPQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O - Trypanosoma cruzi ? - ? 355609 3.4.22.51 2-aminobenzoyl-LGMISLMKRPQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O Abz-LGMISLMKRPQ-EDDnp Trypanosoma cruzi ? - ? 355609 3.4.22.51 2-aminobenzoyl-Phe-Arg-(2,4-dinitrophenyl)-epsilon-NH2-lysine-Pro-OH + H2O - Trypanosoma cruzi ? - ? 383737 3.4.22.51 2-aminobenzoyl-Phe-Arg-(2,4-dinitrophenyl)-epsilon-NH2-lysine-Trp-OH + H2O - Trypanosoma cruzi ? - ? 383738 3.4.22.51 2-aminobenzoyl-RRF-(2,4-dinitrophenyl)-epsilon-NH2-lysine-OH + H2O - Trypanosoma cruzi ? - ? 383741 3.4.22.51 azocasein + H2O - Trypanosoma cruzi ? - ? 15347 3.4.22.51 benzoyl-FR-4-methylcoumarin 7-amide + H2O - Trypanosoma cruzi ? - ? 355611 3.4.22.51 benzoyl-FR-4-methylcoumarin 7-amide + H2O fluorigenic substrates based on this peptide, substitution of Phe results in less susceptible substrates Trypanosoma cruzi ? - ? 355611 3.4.22.51 benzoyl-HR-4-methylcoumarin 7-amide + H2O less susceptible substrate Trypanosoma cruzi ? - ? 355613 3.4.22.51 benzoyl-PFR-4-nitroanilide + H2O - Trypanosoma cruzi ? - ? 355614 3.4.22.51 benzoyl-Phe-Arg-4-methylcoumarin-7-amide + H2O fluorogenic substrates based on the peptide benzoyl-Phe-Arg-4-methylcoumarin-7-amide Trypanosoma cruzi ? - ? 355615 3.4.22.51 benzoyl-Phe-Val-Arg-p-nitroanilide + H2O as active as with benzyloxycarbonyl-Arg-Arg-methoxy-beta-naphthylamide Trypanosoma cruzi ? - ? 384266 3.4.22.51 benzoyl-Pro-Phe-Arg-p-nitroanilide + H2O 41% of the activity with benzyloxycarbonyl-Arg-Arg-methoxy-beta-naphthylamide Trypanosoma cruzi ? - ? 384267 3.4.22.51 benzoyl-RR-4-methylcoumarin 7-amide + H2O less susceptible substrate Trypanosoma cruzi ? - ? 355612 3.4.22.51 benzoyl-Val-Gly-Arg-p-nitroanilide + H2O 52% of the activity with benzyloxycarbonyl-Arg-Arg-methoxy-beta-naphthylamide Trypanosoma cruzi ? - ? 384268 3.4.22.51 benzoyl-YR-4-methylcoumarin 7-amide + H2O - Trypanosoma cruzi ? - ? 355616 3.4.22.51 benzyloxycarbonyl-Ala-Arg-Arg-methoxy-beta-naphthylamide + H2O 36% of the activity with benzyloxycarbonyl-Arg-Arg-methoxy-beta-naphthylamide Trypanosoma cruzi ? - ? 384277 3.4.22.51 benzyloxycarbonyl-Arg-Arg-methoxy-beta-naphthylamide + H2O - Trypanosoma cruzi ? - ? 384279 3.4.22.51 benzyloxycarbonyl-Gly-Pro-Arg-p-nitroanilide + H2O 66% of the activity with benzyloxycarbonyl-Arg-Arg-methoxy-beta-naphthylamide Trypanosoma cruzi ? - ? 384290 3.4.22.51 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O - Trypanosoma cruzi ? - ? 36475 3.4.22.51 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O - Trypanosoma congolense ? - ? 36475 3.4.22.51 benzyloxycarbonyl-Phe-Arg-methoxy-beta-naphthylamide + H2O 30% of the activity with benzyloxycarbonyl-Arg-Arg-methoxy-beta-naphthylamide Trypanosoma cruzi ? - ? 384304 3.4.22.51 Bovine serum albumin + H2O - Trypanosoma cruzi ? - ? 16295 3.4.22.51 casein + H2O - Trypanosoma cruzi ? - ? 15445 3.4.22.51 epsilon-NH2(capryl)-L-(SBzl)C-7-amido-4-methylcoumarin + H2O - Trypanosoma cruzi ? - ? 391916 3.4.22.51 Gelatin + H2O - Trypanosoma rangeli ? - ? 16570 3.4.22.51 Gelatin + H2O pH 5.5 or 10 Trypanosoma cruzi ? - ? 16570 3.4.22.51 Hemoglobin + H2O - Trypanosoma cruzi ? - ? 15450 3.4.22.51 human IgG + H2O specific cleavage sites on human IgG subclasses by cruzipain Trypanosoma cruzi ? - ? 189962 3.4.22.51 human NF-kappaB P65 + H2O - Trypanosoma cruzi ? - ? 417969 3.4.22.51 Immunoglobulin G1 + H2O - Trypanosoma cruzi ? - ? 16999 3.4.22.51 Immunoglobulin G1 + H2O cleaves specifically at the hinge region Trypanosoma cruzi ? - ? 16999 3.4.22.51 Immunoglobulin G3 + H2O - Trypanosoma cruzi ? - ? 17001 3.4.22.51 Immunoglobulin G3 + H2O cleaves specifically at the hinge region Trypanosoma cruzi ? - ? 17001 3.4.22.51 insulin A + H2O - Trypanosoma cruzi ? - ? 355623 3.4.22.51 insulin B + H2O - Trypanosoma cruzi ? - ? 355624 3.4.22.51 KEEASSAVVGGPG + H2O - Trypanosoma cruzi ? - ? 355625 3.4.22.51 KEEASSAVVRGPG + H2O - Trypanosoma cruzi ? - ? 355626 3.4.22.51 additional information substrates are chromogenic peptides with a carboxyl Arg or Lys, enzyme requires at least one more amino acid, preferably Arg, Phe, Val or Leu between the terminal Arg or Lys and the amino-blocking group Trypanosoma cruzi ? - ? 89 3.4.22.51 additional information synthetic chromogenic sustrates Trypanosoma cruzi ? - ? 89 3.4.22.51 additional information no substrate: benzyloxycarbonyl-Arg-Arg-aminomethyl coumarin Trypanosoma congolense ? - ? 89 3.4.22.51 additional information antigenic character in natural human infections Trypanosoma cruzi ? - ? 89 3.4.22.51 additional information stimulates potent humoral and cellular immune response during infection in both humans and mice Trypanosoma cruzi ? - ? 89 3.4.22.51 additional information enzyme plays a role in the production of antibodies against cardiac M2 muscarinic acetylcholine receptor Trypanosoma cruzi ? - ? 89 3.4.22.51 additional information cruzipain may act as a survival factor for cardiomyocytes because it rescues them from apoptosis and stimulates arginase-2 Trypanosoma cruzi ? - ? 89 3.4.22.51 additional information substrate specificity of cruzipain 2 isoform is analysed using internally quenched flurogenic substrates. It is found that cruzipain 2 compared to cruzipain differs substantially regarding the specificity in the S2, S1' and S2' pockets, suggesting that cruzipain 2 acts on distinct natural substrates Trypanosoma cruzi ? - ? 89 3.4.22.51 additional information enzyme may be involved in facilitating parasite entry into the brain Trypanosoma brucei ? - ? 89 3.4.22.51 additional information design of selective prodrugs of primaquine: Lys-Arg-primaquine, Phe-Ala-primaquine, and Phe-Arg-primaquine, cleaved by the enzyme in vivo and selectively inhibiting it, overview Trypanosoma cruzi ? - ? 89 3.4.22.51 additional information design of selective prodrugs of primaquine: Lys-Arg-primaquine, Phe-Ala-primaquine, and Phe-Arg-primaquine, cleaved by the enzyme in vivo and selectively inhibiting it, overview. The molecular mechanism acts via nucleophilic attack of the catalytic Cys25 residue on the respective prodrug amide carbonyl carbon, releasing primaquine, docking study showing specific interactions between the prodrug and the S1 and S2 pockets of cruzipain with the dipeptides acting as distance holders Trypanosoma cruzi ? - ? 89 3.4.22.51 additional information involvement of O-linked GlcNAc moiety present in the major cysteine protease both in cruzipain recognition and in the cross-reactivity between cruzipain and myosin molecules Trypanosoma cruzi ? - ? 89 3.4.22.51 additional information enzyme may be involved in facilitating parasite entry into the brain Trypanosoma brucei 90-13 ? - ? 89 3.4.22.51 N-carbobenzoxy-Val-Val-Arg-4-nitroanilide + H2O - Trypanosoma cruzi ? - ? 355628 3.4.22.51 N-carbobenzoxy-Val-Val-Arg-4-nitroanilide + H2O excellent substrate Trypanosoma cruzi ? - ? 355628 3.4.22.51 N-tert-butoxycarbonyl-O-benzyl-Ser-Gly-Arg-p-nitroanilide + H2O 39% of the activity with benzyloxycarbonyl-Arg-Arg-methoxy-beta-naphthylamide Trypanosoma cruzi ? - ? 385342 3.4.22.51 N-tert-butoxycarbonyl-Val-Leu-Gly-Arg-p-nitroanilide + H2O 24% of the activity with benzyloxycarbonyl-Arg-Arg-methoxy-beta-naphthylamide Trypanosoma cruzi ? - ? 385343 3.4.22.51 o-aminobenzoyl-ALRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Trypanosoma cruzi ? - ? 392968 3.4.22.51 o-aminobenzoyl-HLRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Trypanosoma cruzi ? - ? 392984 3.4.22.51 o-aminobenzoyl-KARFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Trypanosoma cruzi ? - ? 392986 3.4.22.51 o-aminobenzoyl-KFRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Trypanosoma cruzi ? - ? 392987 3.4.22.51 o-aminobenzoyl-KPRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Trypanosoma cruzi ? - ? 393000 3.4.22.51 o-aminobenzoyl-KRRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Trypanosoma cruzi ? - ? 393001 3.4.22.51 o-aminobenzoyl-LLRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Trypanosoma cruzi ? - ? 393002 3.4.22.51 peptidyl-4-nitroanilide + H2O derivatives of Trypanosoma cruzi ? - ? 355629 3.4.22.51 peptidyl-beta-naphthylamide + H2O derivatives of Trypanosoma cruzi ? - ? 355630 3.4.22.51 peptidyl-methylcoumarin amide + H2O and derivatives, enzyme shows preference for benzyl-Cys or Arg at the P1 position and a hydrophobic non-aromatic residue at position P2 Trypanosoma cruzi ? - ? 355631 3.4.22.51 Phe-Arg-AMC - Trypanosoma cruzi ? - ? 406370 3.4.22.51 Z-Phe-Arg-7-amido-4-methylcoumarin + H2O - Trypanosoma brucei rhodesiense ? - ? 385971 3.4.22.51 Z-Phe-Arg-7-amido-4-methylcoumarin + H2O 5 microM, pH 6.5, 28°C Trypanosoma cruzi ? - ? 385971 3.4.22.51 Ac-Phe-Arg-7-amido-4-methylcoumarin + H2O 10 microM, 4 nanoM cruzain, 45 min, room temperature, pH 5.5 Trypanosoma cruzi Ac-Phe-Arg + 7-amino-4-methylcoumarin - ? 404129 3.4.22.51 benzoyl-His-Arg-7-amido-4-methylcoumarin + H2O - Trypanosoma cruzi benzoyl-His-Arg + 7-amino-4-methylcoumarin - ? 453103 3.4.22.51 benzoyl-Phe-Arg-7-amido-4-methylcoumarin + H2O - Trypanosoma cruzi benzoyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 453094 3.4.22.51 benzoyl-Tyr-Arg-7-amido-4-methylcoumarin + H2O - Trypanosoma cruzi benzoyl-Tyr-Arg + 7-amino-4-methylcoumarin - ? 453104 3.4.22.51 benzyloxycarbonyl-Arg-Ala-7-amido-4-methylcoumarin + H2O - Trypanosoma cruzi benzyloxycarbonyl-Arg-Ala + 7-amino-4-methylcoumarin - ? 449647 3.4.22.51 benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O - Trypanosoma cruzi benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin - ? 362355 3.4.22.51 benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O relatively low rates of substrate hydrolysis Trypanosoma rangeli benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin - ? 384296 3.4.22.51 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O - Trypanosoma congolense benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 361886 3.4.22.51 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O - Trypanosoma cruzi benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 361886 3.4.22.51 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O - Trypanosoma cruzi INC-5 benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 361886 3.4.22.51 Bz-Phe-Arg-4-methylcoumaryl-7-amide + H2O - Trypanosoma cruzi Bz-Phe-Arg + 7-amino-4-methylcoumarin - ? 418521 3.4.22.51 Bz-Pro-Phe-Arg-4-nitroanilide + H2O - Trypanosoma cruzi Bz-Pro-Phe-Arg + 4-nitroaniline - ? 384395 3.4.22.51 Lys-Arg-primaquine + H2O - Trypanosoma cruzi Lys-Arg + primaquine - ? 417995 3.4.22.51 N-alpha-benzyloxy-carbonyl-L-phenylalanyl-L-alanine-(7-amido-4-methylcoumarin) + H2O - Trypanosoma cruzi N-alpha-benzyloxy-carbonyl-L-phenylalanyl-L-alanine + 7-amino-4-methylcoumarin - ? 385253 3.4.22.51 N-alpha-benzyloxycarbonyl-L-arginyl-L-alanine-(7-amido-4-methylcoumarin) + H2O - Trypanosoma cruzi N-alpha-benzyloxycarbonyl-L-arginyl-L-alanine + 7-amino-4-methylcoumarin - ? 385255 3.4.22.51 N-alpha-benzyloxycarbonyl-L-tyrosyl-L-alanine-(7-amido-4-methylcoumarin) + H2O - Trypanosoma cruzi N-alpha-benzyloxycarbonyl-L-tyrosyl-L-alanine + 7-amino-4-methylcoumarin - ? 385257 3.4.22.51 N-carbobenzoxy-Arg-Arg-2-(4-methoxy)-naphthylamide + H2O - Trypanosoma cruzi N-carbobenzoxy-Arg-Arg + 2-amino-4-methoxynaphthalene - ? 355627 3.4.22.51 N-carbobenzoxy-VVR-4-nitroanilide + H2O - Trypanosoma cruzi N-carbobenzoxy-VVR + 4-nitroaniline - ? 453101 3.4.22.51 o-aminobenzoyl-KLFFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Trypanosoma cruzi o-aminobenzoyl-KLF + FSKQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392988 3.4.22.51 o-aminobenzoyl-KLGFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Trypanosoma cruzi o-aminobenzoyl-KLG + FSKQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392989 3.4.22.51 o-aminobenzoyl-KLKFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Trypanosoma cruzi o-aminobenzoyl-KLK + FSKQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392990 3.4.22.51 o-aminobenzoyl-KLRFAKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Trypanosoma cruzi o-aminobenzoyl-KLR + FAKQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392991 3.4.22.51 o-aminobenzoyl-KLRFFKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Trypanosoma cruzi o-aminobenzoyl-KLR + FFKQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392992 3.4.22.51 o-aminobenzoyl-KLRFPKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Trypanosoma cruzi o-aminobenzoyl-KLR + FPKQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392993 3.4.22.51 o-aminobenzoyl-KLRFSFQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O no hydrolysis is detected Trypanosoma cruzi o-aminobenzoyl-KLR + FSFQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392994 3.4.22.51 o-aminobenzoyl-KLRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Trypanosoma cruzi o-aminobenzoyl-KLR + FSKQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392995 3.4.22.51 o-aminobenzoyl-KLRFSPQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Trypanosoma cruzi o-aminobenzoyl-KLR + FSPQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392996 3.4.22.51 o-aminobenzoyl-KLRFSRQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Trypanosoma cruzi o-aminobenzoyl-KLR + FSRQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392997 3.4.22.51 o-aminobenzoyl-KLRLSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Trypanosoma cruzi o-aminobenzoyl-KLR + LSKQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392998 3.4.22.51 o-aminobenzoyl-KLRRSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Trypanosoma cruzi o-aminobenzoyl-KLR + RSKQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392999 3.4.22.51 Phe-Ala-primaquine + H2O - Trypanosoma cruzi Phe-Ala + primaquine - ? 418022 3.4.22.51 Phe-Arg-primaquine + H2O - Trypanosoma cruzi Phe-Arg + primaquine - ? 418023 3.4.22.51 tosyl-Gly-Pro-Arg-p-nitroanilide + H2O 25% of the activity with benzyloxycarbonyl-Arg-Arg-methoxy-beta-naphthylamide Trypanosoma cruzi tosyl-Gly-Pro-Arg + p-nitroaniline - ? 362090 3.4.22.51 Z-Phe-Arg-7-amido-4-methylcoumarin + H2O - Trypanosoma cruzi Z-Phe-Arg + 7-amino-4-methylcoumarin - ? 385972