3.4.21.B43	Abz-GMQWLKTQGN-(3-NO2-Tyr) + H2O	-	Homo sapiens	?	-	?	449448	
3.4.21.B43	Abz-ISLMKYRPPGF-(3-NO2)-Tyr + H2O	very low activity	Homo sapiens	?	-	?	431480	
3.4.21.B43	Abz-SPFRSSR-(3-NO2)-Tyr + H2O	low activity	Homo sapiens	?	-	?	431545	
3.4.21.B43	alpha2-antiplasmin + H2O	-	Homo sapiens	?	-	?	391266	
3.4.21.B43	Antithrombin III + H2O	-	Homo sapiens	?	-	?	16579	
3.4.21.B43	Boc-VPR-7-amido-4-methylcoumarin + H2O	-	Homo sapiens	Boc-VPR + 7-amino-4-methylcoumarin	-	?	431707	
3.4.21.B43	C1 inhibitor + H2O	-	Homo sapiens	?	-	?	365844	
3.4.21.B43	CCN1 protein + H2O	i.e. (cyr61,ctgf, nov), the main cleavage site is localized in the hinge region between the first and second half of the recombinant protein at Lys88-Gly89, Lys208-Arg209, Arg218-Ile219, Lys291-Lys292, and Lys345-Asn346	Homo sapiens	?	-	?	417913	
3.4.21.B43	CCN2 protein + H2O	-	Homo sapiens	?	-	?	417914	
3.4.21.B43	CCN3 protein + H2O	-	Homo sapiens	?	-	?	417915	
3.4.21.B43	CCN4 protein + H2O	-	Homo sapiens	?	-	?	417916	
3.4.21.B43	CCN5 protein + H2O	cleavage site is at Arg215-Val216	Homo sapiens	?	-	?	417917	
3.4.21.B43	CCN6 protein + H2O	-	Homo sapiens	?	-	?	417918	
3.4.21.B43	D-Val-Leu-Lys-thiobenzyl ester + H2O	100% activity	Homo sapiens	D-Val-Leu-Lys + thiobenzyl alcohol	-	?	391785	
3.4.21.B43	Fibronectin + H2O	-	Homo sapiens	?	-	?	15394	
3.4.21.B43	Fibronectin + H2O	the enzyme cleaves the human extracellular matrix proteins fibronectin and tenascin, both of which are involved in the regulation of endothelial cell adhesion and migration. KLK12-mediated fibronectin proteolysis antagonizes fibronectin polymerization and fibronectin fibril formation by endothelial cells, leading to an increase in cell migration	Homo sapiens	?	-	?	15394	
3.4.21.B43	high molecular weight kininogen + H2O	proteolysis pattern, overview. Release of bradykinin, a fragment containing the C-terminal end of kinins. The kininogenase activity of the enzyme is poor due to the resistance of hydrolysis of the K-R bond on the N-terminal side of the bradykinin sequence in the kininogen	Homo sapiens	?	-	?	431939	
3.4.21.B43	influenza hemagglutinin precursor + H2O	the enzyme shows a preference for the H1 and H2 subtypes, the amino acids neighboring the arginine cleavage site affect cleavage efficiency	Homo sapiens	mature influenza hemagglutinin + ?	-	?	431033	
3.4.21.B43	influenza hemagglutinin precursor + H2O	the enzyme shows a preference for the H1 and H2 subtypes, essentially no cleavage of the H3 subtype, the amino acids neighboring the arginine cleavage site affect cleavage efficiency	Homo sapiens	mature influenza hemagglutinin + ?	-	?	431033	
3.4.21.B43	additional information	hormonal reglation of enzyme expression, overview	Homo sapiens	?	-	?	89	
3.4.21.B43	additional information	no cleavage is observed for tert-butyloxycarbonyl-QGR-7-amido-4-methylcoumarin, benzyloxycarbonyl-GGR-7-amido-4-methylcoumarin, L-Pro-Phe-Arg-7-amido-4-methylcoumarin, succinyl-AFK-7-amido-4-methylcoumarin, succinyl-AAPF-7-amido-4-methylcoumarin, succinyl-ALPF-7-amido-4-methylcoumarin, succinyl-LLVY-7-amido-4-methylcoumarin, L-Tyr-7-amido-4-methylcoumarin, and S2586	Homo sapiens	?	-	?	89	
3.4.21.B43	additional information	pro-KLK12 can undergo autoactivation	Homo sapiens	?	-	?	89	
3.4.21.B43	additional information	active KLK12 possesses trypsin-like activity, targeting peptide bonds featuring either arginine or lysine at their P1 position	Homo sapiens	?	-	?	89	
3.4.21.B43	additional information	Abz-GPFTDVRAAVY-(3-NO2-Tyr) is not efficiently hydrolyzed	Homo sapiens	?	-	?	89	
3.4.21.B43	platelet-derived growth factor B precursor + H2O	membrane bound zymogen	Homo sapiens	mature platelet-derived growth factor B + ?	soluble mature paracrine factor	?	431109	
3.4.21.B43	platelet-derived growth factor B precursor + H2O	membrane bound zymogen	Homo sapiens	mature platelet-derived growth factor B + ?	soluble mature protein	?	431109	
3.4.21.B43	polypeptide + H2O	-	Homo sapiens	peptides	-	?	361837	
3.4.21.B43	polypeptide + H2O	involved in posttranslational processing of polypeptide precursors	Homo sapiens	peptides	-	?	361837	
3.4.21.B43	protein C inhibitor + H2O	-	Homo sapiens	?	-	?	362136	
3.4.21.B43	tenascin + H2O	-	Homo sapiens	?	-	?	448986	
3.4.21.B43	tenascin + H2O	the enzyme cleaves the human extracellular matrix proteins fibronectin and tenascin, both of which are involved in the regulation of endothelial cell adhesion and migration	Homo sapiens	?	-	?	448986	
3.4.21.B43	tert-butyloxycarbonyl-DPR-7-amido-4-methylcoumarin + H2O	55% activity compared to D-Val-Leu-Lys-thiobenzyl ester	Homo sapiens	tert-butyloxycarbonyl-DPR + 7-amino-4-methylcoumarin	-	?	393649	
3.4.21.B43	tert-butyloxycarbonyl-FSR-7-amido-4-methylcoumarin + H2O	13% activity compared to D-Val-Leu-Lys-thiobenzyl ester	Homo sapiens	tert-butyloxycarbonyl-FSR + 7-amino-4-methylcoumarin	-	?	393650	
3.4.21.B43	tert-butyloxycarbonyl-QAR-7-amido-4-methylcoumarin + H2O	98% activity compared to D-Val-Leu-Lys-thiobenzyl ester	Homo sapiens	tert-butyloxycarbonyl-QAR + 7-amino-4-methylcoumarin	-	?	393653	
3.4.21.B43	tert-butyloxycarbonyl-VPR-7-amido-4-methylcoumarin + H2O	85% activity compared to D-Val-Leu-Lys-thiobenzyl ester	Homo sapiens	tert-butyloxycarbonyl-VPR + 7-amino-4-methylcoumarin	-	?	393654