3.4.21.91 Abz-AAGRK-SLTLnY-amide + H2O - Dengue virus type 2 Abz-AAGRK + SLTLnY-amide - ? 399803 3.4.21.91 Abz-AAGRKSLTL-3-nitrotyrosine-amide + H2O - Dengue virus type 2 Abz-AAGRK + SLTL-3-nitrotyrosine-amide - ? 396133 3.4.21.91 Abz-AGRK-SLTnY-amide + H2O - Dengue virus type 2 Abz-AGRK + SLTnY-amide - ? 399804 3.4.21.91 Abz-AGRK-SnY-amide + H2O - Dengue virus type 2 Abz-AGRK + Ser-(3-nitro)Tyr-amide - ? 399805 3.4.21.91 Abz-AGRKGTGN-3-nitrotyrosine-amide + H2O the poorest substrate Dengue virus type 2 Abz-AGRKGTGN + 3-nitrotyrosine-amide - ? 396134 3.4.21.91 Abz-AGRKGTGNnY-amide + H2O - Dengue virus type 2 Abz-AGRK + GTGNnY-amide - ? 399806 3.4.21.91 Abz-AGRKS-3-nitrotyrosine-amide + H2O - Dengue virus type 2 Abz-AGRKS + 3-nitrotyrosine-amide - ? 396135 3.4.21.91 Abz-AGRKSLT-3-nitrotyrosine-amide + H2O - Dengue virus type 2 Abz-AGRK + SLT-3-nitrotyrosine-amide - ? 396136 3.4.21.91 Abz-ARRK-SLTLnY-amide + H2O - Dengue virus type 2 Abz-ARRK + SLTLnY-amide - ? 399808 3.4.21.91 Abz-ARRKSLTL-3-nitrotyrosine-amide + H2O - Dengue virus type 2 Abz-ARRK + SLTL-3-nitrotyrosine-amide - ? 396137 3.4.21.91 Abz-FAAGRK-SLTLnY-amide + H2O - Dengue virus type 2 Abz-FAAGRK + SLTLnY-amide - ? 399809 3.4.21.91 Abz-FAAGRKSLTL-3-nitrotyrosine-amide + H2O - Dengue virus type 2 Abz-FAAGRK + SLTL-3-nitrotyrosine-amide - ? 396139 3.4.21.91 Abz-GRK-SLTLnY-amide + H2O - Dengue virus type 2 Abz-GRK + SLTLnY-amide - ? 399810 3.4.21.91 Abz-GRK-SnY-amide + H2O - Dengue virus type 2 Abz-GRK + Ser-(3-nitro)Tyr-amide - ? 399811 3.4.21.91 Abz-GRKS-3-nitrotyrosine-amide + H2O - Dengue virus type 2 Abz-GRK + S-3-nitrotyrosine-amide - ? 396146 3.4.21.91 Abz-GRKSLTL-3-nitrotyrosine-amide + H2O - Dengue virus type 2 Abz-GRK + SLTL-3-nitrotyrosine-amide - ? 396147 3.4.21.91 Abz-KKQR-AGVLnY-amide + H2O high degree of cleavage efficiency, based on the NS2B/NS3 polyprotein cleavage site Dengue virus type 2 Abz-KKQR + AGVLnY-amide - ? 399812 3.4.21.91 Abz-KKQR-SAGMnY-amide + H2O - Dengue virus type 2 Abz-KKQR + SAGMnY-amide - ? 399813 3.4.21.91 Abz-KKQRAGVL-3-nitrotyrosine-amide + H2O is based on the NS2B/NS3 polyprotein cleavage site, shows high degree of cleavage efficiency Dengue virus type 2 Abz-KKQR + AGVL-3-nitrotyrosine-amide - ? 396148 3.4.21.91 Abz-KKQRSAGM-3-nitrotyrosine-amide + H2O substrates containing the capsid protein prime side sequence SAGM are efficiently hydrolysed Dengue virus type 2 Abz-KKQR + SAGM-3-nitrotyrosine-amide - ? 396149 3.4.21.91 Abz-NRRRR-SAGMnYamide + H2O - Dengue virus type 2 ? - ? 399816 3.4.21.91 Abz-NRRRRSAGM-3-nitrotyrosine-amide + H2O - Dengue virus type 2 Abz-NRRRR + SAGM-3-nitrotyrosine-amide - ? 396150 3.4.21.91 Abz-RK-SLTLnY-amide + H2O - Dengue virus type 2 Abz-RK + SLTLnY-amide - ? 399817 3.4.21.91 Abz-RK-SnY-amide + H2O - Dengue virus type 2 Abz-RK + SnY-amide - ? 399818 3.4.21.91 Abz-RKS-3-nitrotyrosine-amide + H2O - Dengue virus type 2 Abz-RK + S-3-nitrotyrosine-amide - ? 396151 3.4.21.91 Abz-RKSLTL-3-nitrotyrosine-amide + H2O - Dengue virus type 2 Abz-RK + SLTL-3-nitrotyrosine-amide - ? 396152 3.4.21.91 Abz-RRRR-3-nitrotyrosine-amide + H2O marginal activity Dengue virus type 2 Abz-RRRR + 3-nitrotyrosine-amide - ? 396153 3.4.21.91 Abz-RRRR-nY-amide + H2O - Dengue virus type 2 Abz-RRRR + 3-nitro-L-tyrosine amide - ? 399819 3.4.21.91 Abz-RRRR-SAGMnY-amide + H2O - Dengue virus type 2 Abz-RRRR + SAGMnY-amide - ? 399820 3.4.21.91 Abz-RRRR-SAGnY-amide + H2O efficient substrate of the NS3 protease, based on the Dengue virus capsid protein processing site Dengue virus type 2 Abz-RRRR + SAGnY-amide - ? 399821 3.4.21.91 Abz-RRRR-SLTLnY-amide + H2O - Dengue virus type 2 Abz-RRRR + SLTLnY-amide - ? 399822 3.4.21.91 Abz-RRRR-SnY-amide + H2O - Dengue virus type 2 Abz-RRRR + Ser-(3-nitro)Tyr-amide - ? 399823 3.4.21.91 Abz-RRRRS-3-nitrotyrosine-amide + H2O - Dengue virus type 2 Abz-RRRR + S-3-nitrotyrosine-amide - ? 396154 3.4.21.91 Abz-RRRRSAG-3-nitrotyrosine-amide + H2O most efficiently hydrolysed substrate, which contains the tetrabasic non-prime side sequence RRRR of the dengue virus capsid protein Dengue virus type 2 Abz-RRRR + SAG-3-nitrotyrosine-amide - ? 396155 3.4.21.91 Abz-RRRRSAGM-3-nitrotyrosine-amide + H2O - Dengue virus type 2 Abz-RRRR + SAGM-3-nitrotyrosine-amide - ? 396156 3.4.21.91 Abz-RRRRSLTL-3-nitrotyrosineamide + H2O - Dengue virus type 2 Abz-RRRR + SLTL-3-nitrotyrosineamide - ? 396157 3.4.21.91 Abz-SKKR-AGVLnY-amide + H2O - Dengue virus type 2 Abz-SKKR + AGVLnY-amide - ? 399824 3.4.21.91 Abz-SKKR-SAGMnY-amide + H2O - Dengue virus type 2 Abz-SKKR + SAGMnY-amide - ? 399825 3.4.21.91 Abz-SKKRAGVL-3-nitrotyrosine-amide + H2O - Dengue virus type 2 Abz-SKKR + AGVL-3-nitrotyrosine-amide - ? 396158 3.4.21.91 Abz-SKKRSAGM-3-nitrotyrosine-amide + H2O - Dengue virus type 2 Abz-SKKR + SAGM-3-nitrotyrosine-amide - ? 396159 3.4.21.91 Ac-Arg-Lys-Lys-Arg-7-amido-4-carbamoylmethylcoumarin + H2O - Zika virus ? - ? 449480 3.4.21.91 Ac-D-Arg-Lys-Orn-Arg(Me)-7-amido-4-carbamoylmethylcoumarin + H2O - Zika virus ? - ? 449482 3.4.21.91 Ac-D-Arg-Lys-Orn-Arg-7-amido-4-carbamoylmethylcoumarin + H2O substrate profiling of the P1 position using Ac-Ala-Arg-Leu-X-7-amido-4-carbamoylmethylcoumarin (X is natural or unnatural amino acid) Zika virus ? - ? 449483 3.4.21.91 Ac-D-Lys-Lys-Orn-Arg-7-amido-4-carbamoylmethylcoumarin + H2O - Zika virus ? - ? 449484 3.4.21.91 Ac-Nle-Lys-Lys-Arg-7-amido-4-carbamoylmethylcoumarin + H2O - Zika virus ? - ? 449491 3.4.21.91 Ac-Thr-Arg-Arg-7-amido-4-methylcoumarin + H2O - Dengue virus type 2 Ac-Thr-Arg-Arg + 7-amino-4-methylcoumarin - ? 399850 3.4.21.91 acetyl-Nle-Lys-Arg-Arg-4-methylcoumarin 7-amide + H2O - Dengue virus acetyl-Nle-Lys-Arg-Arg + 7-amino-4-methylcoumarin - ? 431566 3.4.21.91 ADP ATOO-647N + H2O affinity of complete NS3 molecule fused to 18 residues of the NS2B cofactor is 10fold higher than that of NS3 helicase Dengue virus type 4 ? - ? 391193 3.4.21.91 AQRRGRIG + H2O three individual batches of the peptide results in AQRR2GRIG 3%, 2%, and 0% cleavages West Nile virus AQRR + GRIG - ? 391301 3.4.21.91 AQRRGRIG + H2O three individual batches of the peptide results in 45%, 37%, and 36% cleavages Dengue virus ? - ? 391302 3.4.21.91 ATG16L1 + H2O human autophagy-related protein 16-1, high activity Zika virus ? - ? 448455 3.4.21.91 ATG16L1 + H2O human autophagy-related protein 16-1 Zika virus ? - ? 448455 3.4.21.91 ATP ATOO-647N + H2O affinity of complete NS3 molecule fused to 18 residues of the NS2B cofactor is 10fold higher than that of NS3 helicase Dengue virus type 4 ? - ? 391385 3.4.21.91 benzoyl-Nle-Lys-Arg-Arg-4-methylcoumarin 7-amide + H2O - Dengue virus benzoyl-Nle-Lys-Arg-Arg + 7-amino-4-methylcoumarin - ? 381540 3.4.21.91 benzoyl-Nle-Lys-Arg-Arg-4-methylcoumarin 7-amide + H2O - West Nile virus benzoyl-Nle-Lys-Arg-Arg + 7-amino-4-methylcoumarin - ? 381540 3.4.21.91 benzoyl-Nle-Lys-Arg-Arg-7-amido-3-carbamoylmethyl-4-methylcoumarin + H2O - Dengue virus benzoyl-Nle-Lys-Arg-Arg + 7-amino-3-carbamoylmethyl-4-methylcoumarin - ? 381541 3.4.21.91 benzoyl-Nle-Lys-Arg-Arg-7-amido-4-methylcoumarin + H2O - Dengue virus benzoyl-Nle-Lys-Arg-Arg + 7-amino-4-methylcoumarin - ? 449636 3.4.21.91 benzoyl-Nle-Lys-Arg-Arg-7-amido-4-methylcoumarin + H2O - Zika virus benzoyl-Nle-Lys-Arg-Arg + 7-amino-4-methylcoumarin - ? 449636 3.4.21.91 benzoyl-Nle-Lys-Arg-Arg-thiobenzylester + H2O - Dengue virus benzoyl-Nle-Lys-Arg-Arg + thiobenzoic acid - ? 381542 3.4.21.91 benzoyl-Nle-Lys-Thr-Arg-7-amido-3-carbamoylmethyl-4-methylcoumarin + H2O - Dengue virus benzoyl-Nle-Lys-Thr-Arg + 7-amino-3-carbamoylmethyl-4-methylcoumarin - ? 381543 3.4.21.91 benzoyl-Nle-Thr-Arg-Arg-7-amido-3-carbamoylmethyl-4-methylcoumarin + H2O - Dengue virus benzoyl-Nle-Thr-Arg-Arg + 7-amino-3-carbamoylmethyl-4-methylcoumarin - ? 381544 3.4.21.91 benzoyl-norleucyl-lysyl-lysyl-argininyl-7-amido-4-methylcoumarin + H2O - Zika virus benzoyl-norleucyl-lysyl-lysyl-arginine + 7-amino-4-methylcoumarin - ? 449641 3.4.21.91 benzoyl-norleucyl-lysyl-lysyl-argininyl-7-amido-4-methylcoumarin + H2O - Zika virus Brazilian isolate BeH823339 benzoyl-norleucyl-lysyl-lysyl-arginine + 7-amino-4-methylcoumarin - ? 449641 3.4.21.91 benzoyl-Thr-Lys-Arg-Arg-7-amido-3-carbamoylmethyl-4-methylcoumarin + H2O - Dengue virus benzoyl-Thr-Lys-Arg-Arg + 7-amino-3-carbamoylmethyl-4-methylcoumarin - ? 381548 3.4.21.91 benzoyl-Thr-Thr-Arg-Arg-7-amido-3-carbamoylmethyl-4-methylcoumarin + H2O - Dengue virus benzoyl-Thr-Thr-Arg-Arg + 7-amino-3-carbamoylmethyl-4-methylcoumarin - ? 381549 3.4.21.91 benzyloxycarbonyl-Gly-Arg-Arg-4-methylcoumarin 7-amide + H2O - Dengue virus benzyloxycarbonyl-Gly-Arg-Arg + 7-amino-4-methylcoumarin - ? 431677 3.4.21.91 benzyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O substrate of isoform DENV-2 Dengue virus benzyloxycarbonyl-Gly-Arg-Arg + 7-amino-4-methylcoumarin - ? 431664 3.4.21.91 Boc-Gly-Arg-Arg-4-methylcoumaryl-7-amide + H2O - Dengue virus Boc-Gly-Arg-Arg + 7-amino-4-methylcoumarin - ? 418517 3.4.21.91 Boc-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O - Yellow fever virus ? - ? 391525 3.4.21.91 Boc-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O - Dengue virus type 2 Boc-Gly-Arg-Arg + 7-amino-4-methylcoumarin - ? 400571 3.4.21.91 Boc-Gly-L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O - Dengue virus ? - ? 384349 3.4.21.91 butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin + H2O - West Nile virus butyloxycarbonyl-Arg-Val-Arg-Arg + 7-amino-4-methylcoumarin - ? 391536 3.4.21.91 butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin + H2O - West Nile virus NY99 butyloxycarbonyl-Arg-Val-Arg-Arg + 7-amino-4-methylcoumarin - ? 391536 3.4.21.91 Bz-Arg-Arg-4-methylcoumaryl-7-amide + H2O - Dengue virus Bz-Arg-Arg + 7-amino-4-methylcoumarin - ? 391541 3.4.21.91 Bz-Nle-Lys-Arg-Arg-7-amido-4-methylcoumarin + H2O 10fold higher affinity for the tetrapeptide than for the tripeptide Boc-Gly-Arg-Arg-7-amido-4-methylcoumarin, and processes it with 34fold greater efficiency Yellow fever virus ? - ? 391540 3.4.21.91 CFP-LQYTKRGGVLWD-YFP + H2O the artificial substrate contains the natural sequence of the NS2B/NS3 junction and is cleaved efficiently West Nile virus CFP-LQYTKR + GGVLWD-YFP - ? 391613 3.4.21.91 CFP-LQYTKRGGVLWD-YFP + H2O the artificial substrate contains the natural sequence of the NS2B/NS3 junction and is cleaved efficiently West Nile virus NY99 CFP-LQYTKR + GGVLWD-YFP - ? 391613 3.4.21.91 CFP-SAAQRRGRIGRN-YFP + H2O cleavage is barely detectable West Nile virus CFP-SAAQRR + GRIGRN-YFP - ? 391614 3.4.21.91 CFP-SAAQRRGRIGRN-YFP + H2O cleavage is barely detectable West Nile virus NY99 CFP-SAAQRR + GRIGRN-YFP - ? 391614 3.4.21.91 Dengue virus DEN4 polyprotein + H2O involved in processing of downstream nonstructural proteins of the Dengue virus DEN4 polyprotein precursor Dengue virus ? - ? 369542 3.4.21.91 Dengue virus polyprotein + H2O - Dengue virus Dengue virus proteins NS3 and NS5 - ? 15811 3.4.21.91 Dengue virus polyprotein + H2O cleaves NS3/NS4A and NS4B/NS5 junctions, not NS4A/4B junction, cleavage site consists of 2 basic amino acids followed by Gly, Ala or Ser, e.g. Arg-Arg-/-Gly Dengue virus Dengue virus proteins NS3 and NS5 - ? 15811 3.4.21.91 DSSTKRGGSW + H2O each substrate plasmid contains the ER membrane-anchoring domain, a specific cleavage sequence that includes the P6-P1 P19-P49 residues and a fluorescent reporter group (DsRed) West Nile virus DSSTKR + GGSW - ? 417937 3.4.21.91 eIF4G1 protein + H2O human eukaryotic translation initiation factor 4 gamma 1, high activity Zika virus ? - ? 448564 3.4.21.91 eIF4G1 protein + H2O human eukaryotic translation initiation factor 4 gamma 1 Zika virus ? - ? 448564 3.4.21.91 FAEGRRGAAEVLQ + H2O - Yellow fever virus FAEGRR + GAAEVLQ - ? 418611 3.4.21.91 flavivirus polyprotein + H2O - Yellow fever virus ? - ? 366200 3.4.21.91 Flavivirus sp. polyproteins + H2O cleavage sites consist of Lys-Arg, Arg-Lys or Arg-Arg (Dengue virus: Gln-Arg) usually followed by Gln, Ala or Ser, cleavage sites: NS2A/2B, NS2B/3, NS3/4A (not SLE virus), NS4B/5 (not SLE virus) Flavivirus sp. ? - ? 15815 3.4.21.91 Flavivirus sp. polyproteins + H2O involved in processing of downstream nonstructural proteins of the virus polyprotein Flavivirus sp. ? - ? 15815 3.4.21.91 GLKRFGAK + H2O - Dengue virus GLKR + FGAK - ? 392081 3.4.21.91 GLKRGSAK + H2O cleaved with 82% efficiency Dengue virus GLKR + GSAK - ? 392082 3.4.21.91 GLKRGSAK + H2O cleaved with 9% efficiency West Nile virus GLKR + GSAK - ? 392082 3.4.21.91 GLKRTGAK + H2O cleaved with 2% efficiency West Nile virus GLKR + TGAK - ? 392083 3.4.21.91 GLKRTGAK + H2O cleaved with 77% efficiency Dengue virus GLKR + TGAK - ? 392083 3.4.21.91 Gly-Arg-Arg-4-methylcoumarin 7-amide + H2O - Dengue virus type 2 Gly-Arg-Arg + 7-amino-4-methylcoumarin - ? 381972 3.4.21.91 human neural transcription factor Sox2 + H2O the substrate protein exhibits likely cleavage sites for NS2B-NS3pro: K95RLR-A99 and R110PRRK-T115 Zika virus ? - ? 450059 3.4.21.91 KKQRAGVL + H2O cleaved with 11% efficiency Dengue virus ? - ? 392317 3.4.21.91 KLAQRRVFHGVAQ + H2O - Yellow fever virus KLAQRR + VFHGVAQ - ? 418728 3.4.21.91 KPGLKRGGAK + H2O each substrate plasmid contains the ER membrane-anchoring domain, a specific cleavage sequence that includes the P6-P1 P19-P49 residues and a fluorescent reporter group (DsRed) West Nile virus KPGLKR + GGAK - ? 417984 3.4.21.91 L-pGlu-L-Arg-L-Thr-L-Lys-L-Arg-7-amido-4-methylcoumarin + H2O - West Nile virus L-pGlu-L-Arg-L-Thr-L-Lys-L-Arg + 7-amino-4-methylcoumarin - ? 412308 3.4.21.91 L-pGlu-L-Arg-L-Thr-L-Lys-L-Arg-7-amido-4-methylcoumarin + H2O - West Nile virus NY99 L-pGlu-L-Arg-L-Thr-L-Lys-L-Arg + 7-amino-4-methylcoumarin - ? 412308 3.4.21.91 LQYTKRGGVLWD + H2O the artificial substrate contains the natural sequence of the NS2B/NS3 junction and is cleaved efficiently West Nile virus LQYTKR + GGVLWD - ? 392498 3.4.21.91 LQYTKRGGVLWD + H2O the artificial substrate contains the natural sequence of the NS2B/NS3 junction and is cleaved efficiently West Nile virus NY99 LQYTKR + GGVLWD - ? 392498 3.4.21.91 LYKRYGGF + H2O cleaved with 48% efficiency Dengue virus ? - ? 392499 3.4.21.91 MKTGRRGSANGKQ + H2O - Yellow fever virus MKTGRR + GSANGKQ - ? 418842 3.4.21.91 MLKRGMPR + H2O cleaved with 49% efficiency Dengue virus ? - ? 392664 3.4.21.91 additional information viral serine protease consisting of viral protein NS2B and NS3 Yellow fever virus ? - ? 89 3.4.21.91 additional information amino-terminal third of NS3 acts as serine protease on nonstructural viral proteins Dengue virus ? - ? 89 3.4.21.91 additional information amino-terminal third of NS3 acts as serine protease on nonstructural viral proteins Yellow fever virus ? - ? 89 3.4.21.91 additional information amino-terminal third of NS3 acts as serine protease on nonstructural viral proteins Flavivirus sp. ? - ? 89 3.4.21.91 additional information cleavage preference for substrates containing basic amino acids Yellow fever virus ? - ? 89 3.4.21.91 additional information interaction of human brain proteins with the Japanese encephalitis virus NS2B-NS3 protease could be involved in the Japanese encephalitis virus induced pathogenesis Japanese encephalitis virus ? - ? 89 3.4.21.91 additional information NS3 alone triggers the apoptotic pathways involving caspases-8 and -3. Activation of caspase-8 is essential to initiate apoptotic signaling in NS3-expressing cells West Nile virus ? - ? 89 3.4.21.91 additional information strong preference for basic amino acid residues (Arg/Lys) at the P1 position, preference for the P2-4 sites are in the order of Arg > Thr > Gln/Asn/Lys for P2, Lys > Arg > Asn for P3, and Nle > Leu > Lys > Xaa for P4. The prime site substrate specificity is for small and polar amino acids in P1' and P3' Dengue virus ? - ? 89 3.4.21.91 additional information during purification, incomplete autolytic conversion of the 36 kDa NS2B-NS3pro construct into the individual 30 kDa NS3pro and the NS2B sequence (5-6 kDa). Undergoes self-cleavage during expression and purification at the K48G-G site of the glycine linker West Nile virus ? - ? 89 3.4.21.91 additional information NS2/3 auto-cleavage and NS3 protease activity Hepacivirus C ? - ? 89 3.4.21.91 additional information o-aminobenzoyl-EPEKQRTPQDNQQ-N-(2,4-dinitrophenyl)-ethylenediamine, o-aminobenzoyl-AGRRPAQ-N-(2,4-dinitrophenyl)-ethylenediamine and o-aminobenzoyl-AKRRRPQ-N-(2,4-dinitrophenyl)-ethylenediamine are not hydrolyzed Dengue virus ? - ? 89 3.4.21.91 additional information peptides with a similar sequence, including GIKRGETD, LQKRGIVE, LSKRQHPG, LVTAGHGQ, and MLKKGMTT, are completely resistant to proteolysis Dengue virus ? - ? 89 3.4.21.91 additional information peptides with a similar sequence, including GIKRGETD, LQKRGIVE, LSKRQHPG, LVTAGHGQ, and MLKKGMTT, are completely resistant to proteolysis. Peptides GLKRFGAK, KKQRAGVL, LYKRYGGF, MLKRGMPR, and NQKRYGGF are also not cleaved West Nile virus ? - ? 89 3.4.21.91 additional information strict substrate specificity, the (K/R)(K/R)R-GG amino acid motif is optimal. Efficiently cleaves peptides derived from the West Nile virus and Dengue virus capsid protein C, from the NS2A/NS2B junction region and NS4B/NS5 junction region and from NS2B/NS3 West Nile virus ? - ? 89 3.4.21.91 additional information the recombinant NS2B-NS3 protease is subject to autolytic cleavage Yellow fever virus ? - ? 89 3.4.21.91 additional information the recombinant NS2B-NS3 protease is subject to autolytic cleavage Japanese encephalitis virus ? - ? 89 3.4.21.91 additional information the recombinant NS2B-NS3 protease is subject to autolytic cleavage Dengue virus type 2 ? - ? 89 3.4.21.91 additional information the recombinant NS2B-NS3 protease is subject to autolytic cleavage Dengue virus type 4 ? - ? 89 3.4.21.91 additional information the recombinant NS2B-NS3 protease is subject to autolytic cleavage. It catalyzes two autolytic cleavages. The NS2B/NS3 junction is cleaved before protein purification. A second site at Arg4592Gly460 within the C-terminal helicase region of NS3 is cleaved more slowly. Intramolecular cleavage of both the N-terminal site and the internal helicase site of recombinant NS2B-NS3. The artificial substrates CFP-LQYTHMGGVLWD-YFP and CFP-GGGGHMGGVLWD-YFP are not cleaved West Nile virus ? - ? 89 3.4.21.91 additional information preferred substrate size for the dengue virus NS3 protease encompasses the residues from P4 to P3'. The presence of basic residues at the P3 and P4 positions significantly promotes reactivity. Serine is the preferred P1' residue Dengue virus type 2 ? - ? 89 3.4.21.91 additional information preferred substrate size for the Dengue virus NS3 protease encompasses the residues from P4 to P3'. Serine is the preferred P1' residue Dengue virus type 2 ? - ? 89 3.4.21.91 additional information docking studies of the bioactive compounds at the active site of NS2B-NS3 complex also indicate 4-hydroxypanduratin A as potential lead compound for drug development Yellow fever virus ? - ? 89 3.4.21.91 additional information the substrate that corresponds to the NS4B/NS5 junction site is cleaved in trans, whereas the remaining substrates that correspond to the NS2A/NS2B,NS2B/NS3, and NS3/NS4A proprotein junction sites are poorly processed in trans under experimental conditions West Nile virus ? - ? 89 3.4.21.91 additional information enzyme is highly specific toward lysines at the P2 and P3 positions West Nile virus ? - ? 89 3.4.21.91 additional information enzyme is highly specific toward lysines at the P2 and P3 positions Murray Valley encephalitis virus ? - ? 89 3.4.21.91 additional information analysis of substrate specificity at substrate positions P1, P2, P3, and P4, and cleavage site parameters, detailed overview. Enzyme cleavage sequence is Ac-Ala-Arg-Leu-X-7-amido-4-carbamoylmethylcoumarin (X is a natural or nonnatural amino acid). The P4-P1 optimal sequence is D-Arg-Lys-Orn-Arg. Most preferred amino acid residues at P1 are L-Arg(Me) and L-Arg. At P2, the NS2B-NS3 protease displays also a very high level of specificity. The most preferred amino acids are the non-proteinogenic L-Orn (100%) and L-Arg(Z)2 (40%). Natural amino acids interact significantly weaker with the S2 pocket, and only L-Lys and L-Arg are recognized (38% and 17%, respectively). Similarly to the S2 and S1 pockets, the S3 site exhibits very narrow specificity. L-Lys is the most preferentially recognized residue at P3 among all tested amino acids. Other amino acids such as L-Orn, L-Arg, L-Arg(Z)2, L-hArg, L-Agp or L-Phe(guan) are recognized with significantly lower affinity. However, all these amino acids have basic side chains. The S4 site exhibits very broad substrate specificity. Almost all amino acids, including unnatural ones, are recognized at the same level at P4 position Zika virus ? - ? 89 3.4.21.91 additional information identification of 31 human proteins that are cleaved by the NS2B-NS3 protease. The cofactor NS2B is the principal determinant in ZVP substrate selection Zika virus ? - ? 89 3.4.21.91 additional information molecular dynamics simulations of the NS2B/NS3 protease complexes with six peptide substrates (capsid, intNS3, 2A/2B, 4B/5, 3/4A and 2B/3 containing the proteolytic site between P1 and P1' subsites) of DENV type 2 to compare the specificity of the protein-substrate binding recognition Dengue virus type 2 ? - ? 89 3.4.21.91 additional information no activity with human maltose binding protein Zika virus ? - ? 89 3.4.21.91 additional information NS2B-NS3 protease is a trypsin-like protease with a serine protease catalytic triad (His51, Asp75, Ser135) that has a polybasic substrate recognition profile Dengue virus type 2 ? - ? 89 3.4.21.91 additional information screening for potential intracellular substrates of NS3, substrate specificity analysis of recombinant enzyme NS2BLNNS3pr using synthesized FRET-type substrate libraries, peptide library preparation. Profiling of the P1-P4 substrate specificity of the NS2B-NS3 protease using a combinatorial chemistry approach and the split-and-mix method. Arg is the only preferred P1 residue of the enzyme, preference for Val at P4, and Lys at P3 and P2, no activity for Trp, Tyr, Asp, and Pro at P3 position, structure-function analysis, overview Zika virus ? - ? 89 3.4.21.91 additional information the enzyme performs self-cleavage processes viral polyproteins. Protease activity analysis of gZiPro, eZiPro and bZiPro Zika virus ? - ? 89 3.4.21.91 additional information strict substrate specificity, the (K/R)(K/R)R-GG amino acid motif is optimal. Efficiently cleaves peptides derived from the West Nile virus and Dengue virus capsid protein C, from the NS2A/NS2B junction region and NS4B/NS5 junction region and from NS2B/NS3 West Nile virus NY99 ? - ? 89 3.4.21.91 additional information the recombinant NS2B-NS3 protease is subject to autolytic cleavage. It catalyzes two autolytic cleavages. The NS2B/NS3 junction is cleaved before protein purification. A second site at Arg4592Gly460 within the C-terminal helicase region of NS3 is cleaved more slowly. Intramolecular cleavage of both the N-terminal site and the internal helicase site of recombinant NS2B-NS3. The artificial substrates CFP-LQYTHMGGVLWD-YFP and CFP-GGGGHMGGVLWD-YFP are not cleaved West Nile virus NY99 ? - ? 89 3.4.21.91 additional information molecular dynamics simulations of the NS2B/NS3 protease complexes with six peptide substrates (capsid, intNS3, 2A/2B, 4B/5, 3/4A and 2B/3 containing the proteolytic site between P1 and P1' subsites) of DENV type 2 to compare the specificity of the protein-substrate binding recognition Dengue virus type 2 Thailand/NGS-C/1944 ? - ? 89 3.4.21.91 myelin basic protein + H2O - West Nile virus ? - ? 140925 3.4.21.91 myelin basic protein + H2O - West Nile virus NY99 ? - ? 140925 3.4.21.91 N-(2-aminobenzoyl)-L-valyl-L-lysyl-L-lysyl-N-(3-carbamoyl-4-nitrophenyl)-L-argininamide + H2O substrate binding structure in the enzyme's active site (PDB ID 5LC0), modeling. The peptidic substrate with the sequence ABZ-Val-Lys-Lys-Arg-ANB-NH2 is selected as the most efficient peptidic substrate with the nonprimed binding sites of ZIKV NS3 protease Zika virus N-(2-aminobenzoyl)-L-valyl-L-lysyl-L-lysyl-L-arginine + 5-amino-2-nitrobenzamide - ir 450317 3.4.21.91 N-acetyl-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O - Dengue virus type 2 N-acetyl-Gly-Arg-Arg + 7-amino-4-methylcoumarin - ? 450347 3.4.21.91 N-acetyl-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O - Dengue virus type 2 Thailand/16681/1984 N-acetyl-Gly-Arg-Arg + 7-amino-4-methylcoumarin - ? 450347 3.4.21.91 N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O - Yellow fever virus N-alpha-benzoyl-L-arginine + p-nitroaniline - ? 392755 3.4.21.91 N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O - dengue virus type I N-alpha-benzoyl-L-arginine + p-nitroaniline - ? 392755 3.4.21.91 N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O - Langat virus N-alpha-benzoyl-L-arginine + p-nitroaniline - ? 392755 3.4.21.91 N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O - Zika virus N-alpha-benzoyl-L-arginine + p-nitroaniline - ? 392755 3.4.21.91 N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O - Saint Louis encephalitis virus N-alpha-benzoyl-L-arginine + p-nitroaniline - ? 392755 3.4.21.91 N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O - Bussuquara virus N-alpha-benzoyl-L-arginine + p-nitroaniline - ? 392755 3.4.21.91 N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O - Dengue virus type 3 N-alpha-benzoyl-L-arginine + p-nitroaniline - ? 392755 3.4.21.91 N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O no substrate-dependent precipitation for either Alkhurma virus mutants or chimeric proteins Alkhumra hemorrhagic fever virus N-alpha-benzoyl-L-arginine + p-nitroaniline - ? 392755 3.4.21.91 N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O - Yellow fever virus Asibi N-alpha-benzoyl-L-arginine + p-nitroaniline - ? 392755 3.4.21.91 N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O - Dengue virus type 3 Sri Lanka/1266/2000 N-alpha-benzoyl-L-arginine + p-nitroaniline - ? 392755 3.4.21.91 N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O - dengue virus type I D1/H/IMTSSA/98/606 N-alpha-benzoyl-L-arginine + p-nitroaniline - ? 392755 3.4.21.91 N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O - Langat virus TP21 N-alpha-benzoyl-L-arginine + p-nitroaniline - ? 392755 3.4.21.91 N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O - Zika virus Ar-D-41644 N-alpha-benzoyl-L-arginine + p-nitroaniline - ? 392755 3.4.21.91 N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O - Saint Louis encephalitis virus MSI-7 N-alpha-benzoyl-L-arginine + p-nitroaniline - ? 392755 3.4.21.91 N-benzoyl-3-(4-aminocyclohexyl)-L-Ala-L-Arg-4-methylcoumaryl-7-amide + H2O - Dengue virus N-benzoyl-3-(4-aminocyclohexyl)-L-Ala-L-Arg + 7-amino-4-methylcoumarin - ? 392760 3.4.21.91 N-benzoyl-3-(4-piperidinyl)-L-Ala-L-Arg-4-methylcoumaryl-7-amide + H2O - Dengue virus N-benzoyl-3-(4-piperidinyl)-L-Ala-L-Arg + 7-amino-4-methylcoumarin - ? 392761 3.4.21.91 N-benzoyl-3-(trans-aminomethylcyclohexyl)-L-Ala-L-Arg-4-methylcoumaryl-7-amide + H2O - Dengue virus N-benzoyl-3-(trans-aminomethylcyclohexyl)-L-Ala-L-Arg + 7-amino-4-methylcoumarin - ? 392762 3.4.21.91 N-benzoyl-3-pyridyl-L-Ala-L-Arg-4-methylcoumaryl-7-amide + H2O - Dengue virus N-benzoyl-3-pyridyl-L-Ala-L-Arg + 7-amino-4-methylcoumarin - ? 392763 3.4.21.91 N-benzoyl-4-(aminomethyl)-L-Phe-L-Arg-4-methylcoumaryl-7-amide + H2O - Dengue virus N-benzoyl-4-(aminomethyl)-L-Phe-L-Arg + 7-amino-4-methylcoumarin - ? 392765 3.4.21.91 N-benzoyl-4-(aminomethyl-N-isopropyl)-L-Phe-L-Arg-4-methylcoumaryl-7-amide + H2O - Dengue virus N-benzoyl-4-(aminomethyl-N-isopropyl)-L-Phe-L-Arg + 7-amino-4-methylcoumarin - ? 392764 3.4.21.91 N-benzoyl-4-(guanidino)-L-Phe-L-Arg-4-methylcoumaryl-7-amide + H2O - Dengue virus N-benzoyl-4-(guanidino)-L-Phe-L-Arg + 7-amino-4-methylcoumarin - ? 392766 3.4.21.91 N-benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide + H2O - Dengue virus N-benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin - ? 392772 3.4.21.91 NQKRYGGF + H2O cleaved with 7% efficiency Dengue virus ? - ? 392897 3.4.21.91 NRKR + GGPA + H2O two individual batches of the peptide results in 78 and 77% cleavages West Nile virus NRKRGGPA - ? 392899 3.4.21.91 NS2B-3181-polyproteins + H2O effect of mutations within Gly-Ala-Arg-Arg-+-Ser sequence on cis-cleavage (mutations at P1', P1, P2, P3 and P4), the term-+- depicts the point of cleavage Yellow fever virus ? - ? 15814 3.4.21.91 o-aminobenzoyl-AGKKSAQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Dengue virus o-aminobenzoyl-AGKK + SAQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392947 3.4.21.91 o-aminobenzoyl-AGKKSAQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Yellow fever virus o-aminobenzoyl-AGKK + SAQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392947 3.4.21.91 o-aminobenzoyl-AGKRSAQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Dengue virus o-aminobenzoyl-AGKR + SAQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392949 3.4.21.91 o-aminobenzoyl-AGKRSAQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Yellow fever virus o-aminobenzoyl-AGKR + SAQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392949 3.4.21.91 o-aminobenzoyl-AGRKSAQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Dengue virus o-aminobenzoyl-AGRK + SAQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392954 3.4.21.91 o-aminobenzoyl-AGRKSAQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Yellow fever virus o-aminobenzoyl-AGRK + SAQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392954 3.4.21.91 o-aminobenzoyl-AGRRSAQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Dengue virus o-aminobenzoyl-AGRR + SAQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392955 3.4.21.91 o-aminobenzoyl-AGRRSAQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Yellow fever virus o-aminobenzoyl-AGRR + SAQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392955 3.4.21.91 o-aminobenzoyl-AKKKSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Dengue virus o-aminobenzoyl-AKKK + SQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392963 3.4.21.91 o-aminobenzoyl-AKKKSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Yellow fever virus o-aminobenzoyl-AKKK + SQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392963 3.4.21.91 o-aminobenzoyl-AKKRSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Dengue virus o-aminobenzoyl-AKKR + SQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392964 3.4.21.91 o-aminobenzoyl-AKKRSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Yellow fever virus o-aminobenzoyl-AKKR + SQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392964 3.4.21.91 o-aminobenzoyl-AKRKSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Dengue virus o-aminobenzoyl-AKRK + SQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392965 3.4.21.91 o-aminobenzoyl-AKRKSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Yellow fever virus o-aminobenzoyl-AKRK + SQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392965 3.4.21.91 o-aminobenzoyl-AKRRSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Yellow fever virus o-aminobenzoyl-AKRR + SQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392966 3.4.21.91 o-aminobenzoyl-AKRRSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O is most efficiently cleaved. Substrates containing two or three basic amino acids (Arg or Lys) in tandem are best Dengue virus o-aminobenzoyl-AKRR + SQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392966 3.4.21.91 o-aminobenzoyl-ARKKSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Dengue virus o-aminobenzoyl-ARKK + SQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392972 3.4.21.91 o-aminobenzoyl-ARKKSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Yellow fever virus o-aminobenzoyl-ARKK + SQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392972 3.4.21.91 o-aminobenzoyl-ARKRSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Dengue virus o-aminobenzoyl-ARKR + SQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392973 3.4.21.91 o-aminobenzoyl-ARKRSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Yellow fever virus o-aminobenzoyl-ARKR + SQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392973 3.4.21.91 o-aminobenzoyl-ARRKSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Dengue virus o-aminobenzoyl-ARRK + SQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392974 3.4.21.91 o-aminobenzoyl-ARRKSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Yellow fever virus o-aminobenzoyl-ARRK + SQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392974 3.4.21.91 o-aminobenzoyl-ARRRSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Dengue virus o-aminobenzoyl-ARRR + SQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392975 3.4.21.91 o-aminobenzoyl-ARRRSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Yellow fever virus o-aminobenzoyl-ARRR + SQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392975 3.4.21.91 o-aminobenzoyl-EVKKQRAGVLWDQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Dengue virus o-aminobenzoyl-EVKKQR + AGVLWDQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392981 3.4.21.91 o-aminobenzoyl-FAAGRKSLTLNLQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Dengue virus o-aminobenzoyl-FAAGRK + SLTLNLQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392982 3.4.21.91 o-aminobenzoyl-FAAQRRGRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Dengue virus ? - ? 392983 3.4.21.91 o-aminobenzoyl-HRREKRSVALQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O is poorly hydrolyzed Dengue virus o-aminobenzoyl-HRREKR + SVALQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 392985 3.4.21.91 o-aminobenzoyl-KKRSSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Yellow fever virus o-aminobenzoyl-KKR + SSKQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 418976 3.4.21.91 o-aminobenzoyl-KRRSSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Yellow fever virus o-aminobenzoyl-KRR + SSKQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 418977 3.4.21.91 o-aminobenzoyl-LNRRRRTAGMIIQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Dengue virus o-aminobenzoyl-LNRRRR + TAGMIIQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 393003 3.4.21.91 o-aminobenzoyl-RTSKKRSWPLNEQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Dengue virus o-aminobenzoyl-RTSKKR + SWPLNEQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 393004 3.4.21.91 o-aminobenzoyl-SAAGRRGRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Dengue virus ? - ? 393005 3.4.21.91 o-aminobenzoyl-SAAQRKGRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Dengue virus ? - ? 393006 3.4.21.91 o-aminobenzoyl-SAAQRRGLIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Dengue virus ? - ? 393007 3.4.21.91 o-aminobenzoyl-SAAQRRGRIGNNQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Dengue virus ? - ? 393008 3.4.21.91 o-aminobenzoyl-SAAQRRGRIGRLQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Dengue virus ? - ? 393009 3.4.21.91 o-aminobenzoyl-SAAQRRGRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Dengue virus ? - ? 393010 3.4.21.91 o-aminobenzoyl-SAAQRRGRILRNQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Dengue virus ? - ? 393011 3.4.21.91 o-aminobenzoyl-SAAQRRGRTGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Dengue virus ? - ? 393012 3.4.21.91 o-aminobenzoyl-SAAQRRSRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Dengue virus ? - ? 393013 3.4.21.91 o-aminobenzoyl-TTSTRRGTGNIGQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O - Dengue virus o-aminobenzoyl-TTSTRR + GTGNIGQ-N-(2,4-dinitrophenyl)-ethylenediamine - ? 393015 3.4.21.91 PhAc-Lys-Arg-Arg-7-amido-4-methylcoumarin + H2O substrate of isoform DENV-3 Dengue virus PhAc-Lys-Arg-Arg + 7-amino-4-methylcoumarin - ? 432288 3.4.21.91 phenylacetyl-Lys-Lys-Arg-4-nitroanilide + H2O - Dengue virus phenylacetyl-Lys-Lys-Arg + 4-nitroaniline - ? 450461 3.4.21.91 phenylacetyl-Lys-Lys-Arg-4-nitroanilide + H2O chromogenic substrate West Nile virus phenylacetyl-Lys-Lys-Arg + 4-nitroaniline - ? 450461 3.4.21.91 protein C of tick-borne encephalitis virus + H2O the protein contains two amino acid sequence motifs within the carboxy-terminal region that match the canonical NS2B/3 recognition site. The downstream site is necessary and sufficient for efficient cleavage and virion assembly. In contrast, the upstream site is dispensable and placed in a structural context that renders it largely inaccessible to the viral protease. The NS2B/3 protease predominantly uses the downstream cleavage motif R95-R96-S97 for cleavage Flavivirus sp. ? - ? 406507 3.4.21.91 Pyr-Arg-Thr-Lys-Arg-4-methylcoumarin 7-amide + H2O - Murray Valley encephalitis virus Pyr-Arg-Thr-Lys-Arg + 7-amino-4-methylcoumarin - ? 419020 3.4.21.91 Pyr-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin + H2O - West Nile virus Pyr-Arg-Thr-Lys-Arg + 7-amino-4-methylcoumarin - ? 432328 3.4.21.91 Pyr-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin + H2O - Zika virus Pyr-Arg-Thr-Lys-Arg + 7-amino-4-methylcoumarin - ? 432328 3.4.21.91 Pyr-RTKR-7-amido-4-methylcoumarin + H2O - West Nile virus ? - ? 385642 3.4.21.91 pyroglutamic acid-Arg-Thr-Lys-Arg-4-methylcoumarin 7-amide + H2O - West Nile virus pyroglutamic acid-Arg-Thr-Lys-Arg + 7-amino-4-methylcoumarin - ? 432329 3.4.21.91 pyroglutamic acid-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin + H2O - West Nile virus ? - ? 393392 3.4.21.91 pyroglutamic acid-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin + H2O - Dengue virus type 2 ? - ? 393392 3.4.21.91 pyroglutamic acid-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin + H2O efficiently cleaved West Nile virus ? - ? 393392 3.4.21.91 pyroglutamic acid-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin + H2O - Dengue virus type 2 16681 ? - ? 393392 3.4.21.91 pyroglutamic acid-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin + H2O - West Nile virus NY99 ? - ? 393392 3.4.21.91 pyroglutamic acid-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin + H2O - West Nile virus pyroglutamic acid-Arg-Thr-Lys-Arg + 7-amino-4-methylcoumarin - ? 393393 3.4.21.91 pyroglutamic acid-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin + H2O - West Nile virus NY99 pyroglutamic acid-Arg-Thr-Lys-Arg + 7-amino-4-methylcoumarin - ? 393393 3.4.21.91 pyroglutamic acid-L-Arg-L-Thr-L-Lys-L-Arg-7-amido-4-methylcoumarin + H2O - West Nile virus pyroglutamic acid-L-Arg-L-Thr-L-Lys-L-Arg + 7-amino-4-methylcoumarin - ? 412791 3.4.21.91 QRRRGGTA + H2O two individual batches of the peptide results in 58% cleavage West Nile virus QRRR + GGTA - ? 393401 3.4.21.91 RIFGRRSIPVNEQ + H2O - Yellow fever virus RIFGRR + SIPVNEQ - ? 419037 3.4.21.91 SAAQRRGRIGRNQ + H2O - Yellow fever virus SAAQRRGRIGRNQ - ? 419065 3.4.21.91 SSRKRRSHDVLTQ + H2O - Yellow fever virus SSRKRR + SHDVLTQ - ? 419082 3.4.21.91 t-butoxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin + H2O - West Nile virus ? - ? 365865 3.4.21.91 t-butoxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin + H2O - West Nile virus NY99 ? - ? 365865 3.4.21.91 t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O - Yellow fever virus t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin - ? 393632 3.4.21.91 t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O - dengue virus type I t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin - ? 393632 3.4.21.91 t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O - Langat virus t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin - ? 393632 3.4.21.91 t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O - Zika virus t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin - ? 393632 3.4.21.91 t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O - Saint Louis encephalitis virus t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin - ? 393632 3.4.21.91 t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O - Bussuquara virus t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin - ? 393632 3.4.21.91 t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O - Dengue virus type 3 t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin - ? 393632 3.4.21.91 t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O - Yellow fever virus Asibi t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin - ? 393632 3.4.21.91 t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O - Dengue virus type 3 Sri Lanka/1266/2000 t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin - ? 393632 3.4.21.91 t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O - dengue virus type I D1/H/IMTSSA/98/606 t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin - ? 393632 3.4.21.91 t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O - Langat virus TP21 t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin - ? 393632 3.4.21.91 t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O - Zika virus Ar-D-41644 t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin - ? 393632 3.4.21.91 t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O - Saint Louis encephalitis virus MSI-7 t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin - ? 393632 3.4.21.91 t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O - Yellow fever virus t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin - ? 393633 3.4.21.91 t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O - dengue virus type I t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin - ? 393633 3.4.21.91 t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O - Langat virus t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin - ? 393633 3.4.21.91 t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O - Zika virus t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin - ? 393633 3.4.21.91 t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O - Saint Louis encephalitis virus t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin - ? 393633 3.4.21.91 t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O - Bussuquara virus t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin - ? 393633 3.4.21.91 t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O - Dengue virus type 3 t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin - ? 393633 3.4.21.91 t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O - Yellow fever virus Asibi t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin - ? 393633 3.4.21.91 t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O - Dengue virus type 3 Sri Lanka/1266/2000 t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin - ? 393633 3.4.21.91 t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O - dengue virus type I D1/H/IMTSSA/98/606 t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin - ? 393633 3.4.21.91 t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O - Langat virus TP21 t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin - ? 393633 3.4.21.91 t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O - Zika virus Ar-D-41644 t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin - ? 393633 3.4.21.91 t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O - Saint Louis encephalitis virus MSI-7 t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin - ? 393633 3.4.21.91 tert-butoxycarbonyl-Gly-Arg-Arg-4-methylcoumarin 7-amide + H2O - Dengue virus tert-butoxycarbonyl-Gly-Arg-Arg + 7-amino-4-methylcoumarin - ? 382875 3.4.21.91 VRGARRSGDVLWQ + H2O - Yellow fever virus VRGARR + SGDVLWQ - ? 419194 3.4.21.91 Yellow fever virus protein + H2O - Yellow fever virus ? - ? 15812 3.4.21.91 Yellow fever virus protein + H2O cleavage sites: 2A/2B, 2B/3, 3/4A and 4B/5, 4 dibasic sites in nonstructural region of viral polyprotein Yellow fever virus ? - ? 15812 3.4.21.91 YF17D virus protein + H2O cleavage site: Gln-Gln-Arg-+-Ser, i.e. 4A/2K site, this cleavage is prerequisite for processing at the downstream signalase site, determinants of cleavage site specificity for 2B/3 cleavage (site: Gly-Ala-Arg-Arg-+-Ser), the term-+- depicts the point of cleavage Yellow fever virus ? - ? 15813